Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,033,683 A→C L497R (CTG→CGG)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6830AC99.2% 395.6 / NA 122L497R (CTG→CGG) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (65/56);  minor base G (1/0);  total (66/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 122 total aligned reads displayed.

AGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGC  >  NC_000913/3033537‑3033828
                                                                                                                                                  |                                                                                                                                                 
 gTCCGTGGATGGTGATGCGGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATGTACCCAGCTTAAATAAAAAAACCCCGACATTTCCCGGGGTTGTGAGCATAACGTAATGCTTATTTTCCCGGACGCATCGCCGGCAACCGaa                                                                                                                                                >  2:935147/1‑149 (MQ=255)
  tCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCGTCGCCGGGAACCGAAt                                                                                                                                               >  1:417464/1‑149 (MQ=255)
  tCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAAt                                                                                                                                               >  1:92330/1‑149 (MQ=255)
   ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATa                                                                                                                                              >  1:1015932/1‑149 (MQ=255)
    cGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGAGTATCTGCATTTACCCAGCTTAAAGAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATaa                                                                                                                                             <  2:420060/149‑1 (MQ=255)
     gTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAAc                                                                                                                                            <  1:309473/149‑1 (MQ=255)
           ggTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGCACCGAACAACGTcgcg                                                                                                                                      >  1:583286/1‑149 (MQ=255)
           ggTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTcgcg                                                                                                                                      >  2:1414993/1‑149 (MQ=255)
           ggTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTcgcg                                                                                                                                      >  2:537945/1‑149 (MQ=255)
            gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGGGGATCTTCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCCGGAACCGAATAACGCCGCgg                                                                                                                                     >  1:757761/1‑149 (MQ=255)
             tGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGa                                                                                                                                    <  1:320795/149‑1 (MQ=255)
             tGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGGTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACAGCGCGGa                                                                                                                                    >  2:881555/1‑149 (MQ=255)
                tGCAGGGGACCAAGCGCGAAGCCAGAAAACGATTATCTGCATTTGCCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATgg                                                                                                                                 <  2:1245427/149‑1 (MQ=255)
                 gCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGt                                                                                                                                >  1:1554456/1‑149 (MQ=255)
                 gCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAATTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGACCGCATCGCCGGGTACCGAATAACGTCGAGGATggg                                                                                                                                >  1:1373942/1‑148 (MQ=255)
                   aGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTGGCCGGGGTTGTGAGCATAACGTAATGATTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCCCGGATGGTAt                                                                                                                              >  2:588292/1‑149 (MQ=255)
                    ggCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGAAACCAAATAACGTCGCGGCTgggaag                                                                                                                             >  1:1048220/1‑145 (MQ=255)
                     gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATgg                                                                                                                            <  2:1424877/149‑1 (MQ=255)
                     gCGACAAATCGCCAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATgg                                                                                                                            <  1:17483/149‑1 (MQ=255)
                       gACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCt                                                                                                                          >  2:1473789/1‑149 (MQ=255)
                        aCAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTg                                                                                                                         >  1:1366870/1‑149 (MQ=255)
                         cAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGt                                                                                                                        <  1:644661/149‑1 (MQ=255)
                         cAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGt                                                                                                                        >  1:893807/1‑149 (MQ=255)
                          aaaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCCAATAACGTAGCGGATGGTATGGCTGtt                                                                                                                       >  1:67665/1‑149 (MQ=255)
                            aacgcgAAGCCAGAAAACCATTATCTGCCTTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTgg                                                                                                                     <  2:1382904/147‑1 (MQ=255)
                             tCGCGAAGCCAGAAAACGATTATCTTCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTg                                                                                                                         >  1:939667/1‑144 (MQ=255)
                             tCGCGAAGCCAGAAAACGATTATCTTCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTg                                                                                                                         <  2:939667/144‑1 (MQ=255)
                             tCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                                                                                    >  2:648071/1‑149 (MQ=255)
                              cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTg                                                                                                                   <  1:303111/149‑1 (MQ=255)
                                cgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa                                                                                                                 >  2:868184/1‑149 (MQ=255)
                                     ccAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa                                                                                                            <  2:94882/149‑1 (MQ=255)
                                     ccAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGGTGGTGAACATCa                                                                                                            >  1:72955/1‑149 (MQ=255)
                                      cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt                                                                                                           >  1:1202560/1‑149 (MQ=255)
                                       aGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGACCCGAATACCGTCGCGGATGGTATGGCTGTTGGTGAACAGCCtt                                                                                                          >  1:580346/1‑149 (MQ=255)
                                       aGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                                                                          >  1:801051/1‑149 (MQ=255)
                                       aGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTAGGGCTGGTGGTGAACAGCAtt                                                                                                          >  1:110170/1‑149 (MQ=255)
                                           aaCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                                                                                      <  1:882371/149‑1 (MQ=255)
                                              gATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAGTGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGACTGTTAGTGAACAGCATTACCATAc                                                                                                   >  1:1486614/1‑149 (MQ=255)
                                              gATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGACCCGAATAAGGTGGCGGATGGTATGGCTGTTGGTGAACAGCATGACCATAc                                                                                                   >  1:1450658/1‑149 (MQ=255)
                                                ttATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTAATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGGTACCAGCGTTACCATCCgg                                                                                                 >  1:267498/1‑149 (MQ=255)
                                                   tCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGGGAACAGCATTACCATACGGTCg                                                                                              >  1:631019/1‑149 (MQ=255)
                                                   tCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACAGCATTACCATACGGTCg                                                                                              >  1:1392531/1‑149 (MQ=255)
                                                    cTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                                                                             >  1:1087579/1‑149 (MQ=255)
                                                      gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTGGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGtacttg                                                                                           <  2:964227/149‑6 (MQ=255)
                                                          ttACCCAGCTTAAATAAAAAAACCCCGACATTTGCCTGGGTTTTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATGCGGTCGATACCaa                                                                                       <  1:1097644/149‑1 (MQ=255)
                                                             cccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATTCCAATTc                                                                                    >  2:1385581/1‑149 (MQ=255)
                                                             cccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTc                                                                                    >  1:1405033/1‑149 (MQ=255)
                                                              ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                                                   >  1:1269382/1‑149 (MQ=255)
                                                              ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                                                                                   >  1:1395016/1‑149 (MQ=255)
                                                              ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCCATACCAATTcc                                                                                   >  1:1269388/1‑149 (MQ=255)
                                                                aGCTTAACTAAAAAAACCCCCACCTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                                                                                 <  1:251922/149‑1 (MQ=255)
                                                                aGCTTAAATACAAAACCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                                                                                 <  1:1507555/149‑1 (MQ=255)
                                                                aGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                                                                                 >  2:323847/1‑149 (MQ=255)
                                                                 gCTTAAACAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAg                                                                                <  1:570453/149‑1 (MQ=255)
                                                                      aataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                           <  2:786245/149‑1 (MQ=255)
                                                                      aataaaAAAACCCCGACATCTGCCGGGGTTGTGCGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                                                                           <  1:554586/149‑1 (MQ=255)
                                                                             aaaCCCCGACGTTTGCCGGGGTTGGGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                                                    <  1:1042927/149‑1 (MQ=255)
                                                                              aaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGAGGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAAAGCATGACCATAGGGGCGATACCACTTCCTCGCACTGCTGTcggc                                                                   >  2:833027/1‑149 (MQ=255)
                                                                              aaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCACTTCCCAGACCTGCTGTcggc                                                                   >  2:360532/1‑149 (MQ=255)
                                                                               aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                                  <  2:1414937/149‑1 (MQ=255)
                                                                                  ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACCGCATTACCATACGGTCGATACCAATTCCCAGCCCCGCTGTCGGCGGTa                                                               >  2:666182/1‑149 (MQ=255)
                                                                                   cGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa                                                              <  2:632310/149‑1 (MQ=255)
                                                                                   cGACATTTGCCGGGGTTGTGAGCAGAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa                                                              <  2:632315/149‑1 (MQ=255)
                                                                                        tttGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                                                         >  1:787203/1‑149 (MQ=255)
                                                                                           gCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt                                                      <  1:1029163/149‑1 (MQ=255)
                                                                                              ggggTTTGGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGAGAACCGAATAACGTCGCGTATGGTATGTCTTTTCTTGAACAGTATTACCTTACGGTCGATACCAATTCCCATACCTGCTGTCGGCGGTAGGCCATGTTCCa                                                   <  2:294890/149‑1 (MQ=255)
                                                                                                ggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGt                                                 <  2:1202560/149‑1 (MQ=255)
                                                                                                    gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAGGTTCCAGTGCgg                                             >  1:301329/1‑149 (MQ=255)
                                                                                                    gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCGGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCgg                                             >  1:301546/1‑149 (MQ=255)
                                                                                                     tgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt                                            >  2:1370232/1‑149 (MQ=255)
                                                                                                     cgAGCTTATCGGAATGCTTATTTTACCGGACTGATCGCCGGGAACCGGATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt                                            <  2:135444/148‑1 (MQ=255)
                                                                                                       aGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa                                          <  2:1366870/149‑1 (MQ=255)
                                                                                                       aGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAAAGTTTCCGATCGTCTGTCTTGTTGTGAACCGCATTACCATACGGTCGATACCCATTCCAAGACCTGCTGTCGGCGGTAAGCCCTTTTCCCGTTCggggg                                          >  2:1338264/1‑146 (MQ=255)
                                                                                                        gCATAATGTGATGCTTATTTCACCGGACGCATCGGCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTCGGTGAACAGCATTACCATACGGTCGATACCAATTCCCCGTCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc                                         <  2:1269382/149‑1 (MQ=255)
                                                                                                        gCATAAGGTAATGCTGCTTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc                                         <  2:1269388/149‑1 (MQ=255)
                                                                                                        gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc                                         >  1:557863/1‑149 (MQ=255)
                                                                                                          aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGGGCCGt                                       >  1:1485590/1‑149 (MQ=255)
                                                                                                          aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGAAGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt                                       >  1:1411586/1‑149 (MQ=255)
                                                                                                                tAATGCTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt                                 >  2:1110645/1‑149 (MQ=255)
                                                                                                                   ttgctATTTTACCGGAGGCATCGCCCGGACCCGGAGACCGCCGCGGTTGGTCTGGCTGTTGGTGACCAGCATTACCAGACGGTCGAATCCAATTACCAGAGCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                              <  2:263210/145‑1 (MQ=255)
                                                                                                                     cTTATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCCGACCTGCTGTCGGCGGTAAGCCATGTGCCAGTGCGGTGACGTCACCTCCATCg                            >  2:496116/1‑149 (MQ=255)
                                                                                                                      ttATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAGTCTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAGGTTCCATTGCGGTGACGTAATCTTCATCGt                           >  1:1222121/1‑149 (MQ=255)
                                                                                                                      ttATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGCTACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATCTTCCAGTGCGGTGACGTACTCTTCATCGt                           >  2:1198477/1‑149 (MQ=255)
                                                                                                                      ttATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAGTCTTCATCGt                           >  1:414233/1‑149 (MQ=255)
                                                                                                                      ttATTTTACCGGACGAATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGCCGATACCAAATCCCAGACCTGCTGTCGCCGCTAAGCCATGTTCCCGTGCGGTGAGGTAATCTTCATCgc                           >  2:741819/1‑148 (MQ=255)
                                                                                                                       tATTTTACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTa                          <  2:951045/149‑1 (MQ=255)
                                                                                                                         ttttACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa                        >  1:1428672/1‑149 (MQ=255)
                                                                                                                           ttACCGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGCCTGGCGGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc                      <  1:291581/149‑1 (MQ=255)
                                                                                                                             aCCGGACGCATCGCCGTGAACGGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCGGTGCGGTGACGTAATCTTCATCGTAGAAcgt                    >  2:1146571/1‑147 (MQ=255)
                                                                                                                              ccGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                   <  2:971632/149‑1 (MQ=255)
                                                                                                                               cGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGc                                               >  1:106069/1‑120 (MQ=255)
                                                                                                                               cGGACGCATCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGc                                               <  2:106069/120‑1 (MQ=255)
                                                                                                                                      ttCGCCGGGAACCGAAAATCGGCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc           <  2:217890/148‑1 (MQ=255)
                                                                                                                                      ctCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc           <  1:628019/148‑1 (MQ=255)
                                                                                                                                      aTCGCGGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc           <  2:933233/149‑1 (MQ=255)
                                                                                                                                      aTCGCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc           <  1:360532/149‑1 (MQ=255)
                                                                                                                                       tCGCCGGGAACCGAATAACGTCGCGGATGGTATTTCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGGCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTACTCTTCATCGTAGAACATCGCTtcgtcg          >  2:689301/1‑149 (MQ=255)
                                                                                                                                         gCCGGGAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc        <  1:1287597/149‑1 (MQ=255)
                                                                                                                                             ggAACCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt    <  2:787203/149‑1 (MQ=255)
                                                                                                                                               aaCCGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGc  <  1:909431/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGC  >  NC_000913/3033537‑3033828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: