Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,181,724 A→G 5.4% M1T (ATG→ACG) † ymfA ← DUF3592 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,181,7240AG 5.4% 219.2 / 2.5 74M1T (ATG→ACG) †ymfADUF3592 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (43/27);  new base G (2/2);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

TGCCAGACGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAA  >  NC_000913/1181577‑1181868
                                                                                                                                                   |                                                                                                                                                
tGCCAGACGGTATCAACAATGACGCCTTCTGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCAt                                                                                                                                                 <  1:1036032/149‑1 (MQ=255)
tGCCAGACGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCAt                                                                                                                                                 <  2:432363/149‑1 (MQ=255)
tGCCAGACGGTATCAACAATGACGCATTCGCTATGAATCGCGTCTTTGCTATAGGGGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTGCCAGACCGATACCGAGGAAAAGACGNAAANANGCTGTACTCTCCTGACTCAt                                                                                                                                                 >  1:595607/1‑149 (MQ=255)
  ccAGACGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTa                                                                                                                                               >  1:57250/1‑149 (MQ=255)
    aGACGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGc                                                                                                                                             <  1:171527/149‑1 (MQ=255)
     gACGGTATCAACAATGACGCCCTCGGTAGGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACATAAATGAGAATCAGTTCCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGcc                                                                                                                                            <  2:1088610/149‑1 (MQ=255)
     gACGGTATAAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGGACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGcc                                                                                                                                            <  2:215177/149‑1 (MQ=255)
      aCGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCca                                                                                                                                           <  1:654114/149‑1 (MQ=255)
        ggTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCcaca                                                                                                                                         >  1:923725/1‑149 (MQ=255)
          tATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACCCATTAGCCACATc                                                                                                                                       >  1:1056052/1‑149 (MQ=255)
           aTCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCCGTTCCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTGGCAACATcc                                                                                                                                      >  1:270421/1‑149 (MQ=255)
            tCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCAACATCCt                                                                                                                                     >  1:588277/1‑149 (MQ=255)
                caaTGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGct                                                                                                                                 <  1:871955/149‑1 (MQ=255)
                          ttCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTaaaaaa                                                                                                                       >  1:1075390/1‑149 (MQ=255)
                                    aTCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGaaa                                                                                                             >  1:1035001/1‑149 (MQ=255)
                                        cgTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATacc                                                                                                         <  2:57250/149‑1 (MQ=255)
                                         gTCTTTGCTATATGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATacca                                                                                                        <  1:907422/149‑1 (MQ=255)
                                            tttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATCCCAccg                                                                                                     >  1:890910/1‑149 (MQ=255)
                                             ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCGTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgc                                                                                                    >  1:521984/1‑149 (MQ=255)
                                             ttGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCGTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgc                                                                                                    >  1:321501/1‑149 (MQ=255)
                                               gCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgccc                                                                                                  <  1:1067791/149‑1 (MQ=255)
                                                 tataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcccgc                                                                                                >  1:76718/1‑149 (MQ=255)
                                                 tataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGGACTATACCGATAACGTGCAATATATAGAAAAAAACTTTACAGTTCTGGCACATTAGATACACACTTGCTAATTAAAAAACGGGAGGTAAAACCAccgccagc                                                                                                >  2:456828/1‑149 (MQ=255)
                                                 tataGGTGAACTGGTTATAAAAGACAAAAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCAccgcccgc                                                                                                >  2:230503/1‑149 (MQ=255)
                                                  ataGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCt                                                                                               >  1:931346/1‑149 (MQ=255)
                                                   taGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCGTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCtt                                                                                              <  2:321501/149‑1 (MQ=255)
                                                   gagggtaaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCGTTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCtt                                                                                              <  2:521984/143‑1 (MQ=255)
                                                         aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGaa                                                                                        >  2:1020670/1‑149 (MQ=255)
                                                         aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGaa                                                                                        <  2:603946/149‑1 (MQ=255)
                                                         aaCTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGaa                                                                                        >  2:434580/1‑149 (MQ=255)
                                                         aaCTGGCTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACCCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGaa                                                                                        <  2:432107/149‑1 (MQ=255)
                                                             ggTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTaa                                                                                    <  1:935192/149‑1 (MQ=255)
                                                              gTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACACATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCCCCGCCCGCTTGCTGAATTAAc                                                                                   >  2:121711/1‑149 (MQ=255)
                                                              gTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACAGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAACAAACGGGCGGTAATACCACCGCCCGCTGGCTGAATTAAc                                                                                   >  2:560165/1‑149 (MQ=255)
                                                                         caaaaacaGAAAGGGGGACCAGTACCAGACCGAGACCGAGGAATATACGAAAGAAAACTTTACTGGCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTc                                                                        <  1:402594/149‑1 (MQ=255)
                                                                          aacaacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCa                                                                       >  1:831437/1‑149 (MQ=255)
                                                                            caacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGATAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGc                                                                     >  2:596087/1‑149 (MQ=255)
                                                                             aacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCCGCt                                                                    >  1:1059283/1‑149 (MQ=255)
                                                                             aacaGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGGCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCt                                                                    >  2:103761/1‑149 (MQ=255)
                                                                                ataaaTGAGGATCAGTACCAGATCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCt                                                                 <  2:372606/147‑1 (MQ=255)
                                                                                aGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAACACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCt                                                                 <  2:1079603/149‑1 (MQ=255)
                                                                                aGAAATGAGGAGCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCt                                                                 <  1:1041728/149‑1 (MQ=255)
                                                                                 gAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTg                                                                <  1:895127/149‑1 (MQ=255)
                                                                                 gAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTg                                                                >  2:623994/1‑149 (MQ=255)
                                                                                  aaaTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGa                                                               <  1:748033/149‑1 (MQ=255)
                                                                                    aTGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACCTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGata                                                             <  2:923725/149‑1 (MQ=255)
                                                                                         gATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATAct                                                        >  2:1089651/1‑149 (MQ=255)
                                                                                          aTCAGTACCAGCCCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGTGGTAAGACTACCGCCCGCTTGATGCATTAACGTCCTGCTTTCAGCTTCTGATAATActg                                                       >  1:72003/1‑148 (MQ=255)
                                                                                          aTCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATActc                                                       <  1:1027034/149‑1 (MQ=255)
                                                                                              gTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTCCTGATAATACTCTTCa                                                   >  2:499963/1‑149 (MQ=255)
                                                                                               tACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATAATCTTCAt                                                  >  1:714297/1‑149 (MQ=255)
                                                                                                 ccAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATaa                                                <  1:420788/149‑1 (MQ=255)
                                                                                                   aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                              <  1:456828/149‑1 (MQ=255)
                                                                                                   aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                              >  1:86575/1‑149 (MQ=255)
                                                                                                   aGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAt                                              >  2:1079787/1‑149 (MQ=255)
                                                                                                       cGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAAtgctg                                          >  1:413438/1‑149 (MQ=255)
                                                                                                       cGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCCTCATCAAtgctc                                          >  1:7697/1‑148 (MQ=255)
                                                                                                       cGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTGGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTCCTGATAATACTCTTCATAAAtgctc                                          >  2:66186/1‑148 (MQ=255)
                                                                                                            ccGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggc                                     >  2:898350/1‑149 (MQ=255)
                                                                                                             cGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATACTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggct                                    >  2:757804/1‑149 (MQ=255)
                                                                                                              gAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTgctggctg                                   >  2:779230/1‑149 (MQ=255)
                                                                                                               aGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTgc                                  <  1:1092862/149‑1 (MQ=255)
                                                                                                                   atatACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGccc                              >  2:424421/1‑148 (MQ=255)
                                                                                                                      taCGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGATAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACg                           >  1:769172/1‑149 (MQ=255)
                                                                                                                         gaaagaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCa                        >  2:177591/1‑149 (MQ=255)
                                                                                                                          aaagaaaACTTTACTGTTCTGACTCATTAGCCTCATCCTTGCTAACTAAAAAACGGGCGGTAATACCATCGCCCGCTTACTGAAGTAACGTCCTGGTTTCAGCTTCTGATAATCTTCTTCATAAATGCTTCTGGCTGCGCCAGAGTCAt                       >  2:228438/1‑149 (MQ=255)
                                                                                                                           aagaaaACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGGAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCAtt                      >  1:764470/1‑149 (MQ=255)
                                                                                                                                  cTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCAt               >  2:921668/1‑149 (MQ=255)
                                                                                                                                   ttttCTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATCATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCAtt              >  2:202609/1‑149 (MQ=255)
                                                                                                                                     tACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCGTGCGTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTCCGCCAACGTCATTCTGCCGttgg            >  2:163690/1‑147 (MQ=255)
                                                                                                                                        tGTTCTGATGCATTAGCCACATGCTTTCGAACTAAANAACGGGTGGTAATACCACCGCCCGCGTGCTGAATTAACATCCTGCGTTCAGCTTCTTATACTNCTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCa         <  2:595607/149‑1 (MQ=255)
                                                                                                                                             tGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGATTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTATGCCATTCGCCAttttt    >  1:333112/1‑149 (MQ=255)
                                                                                                                                               gcTCATTAGCCACCTCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTaa  <  2:270421/148‑1 (MQ=255)
                                                                                                                                               aCTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTaa  <  2:588277/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TGCCAGACGGTATCAACAATGACGCCTTCGGTATGAATCGCGTCTTTGCTATAGGTGAACTGGTTATAAAAGACAACAACAGAAATGAGGATCAGTACCAGACCGATACCGAGGAATATACGAAAGAAAACTTTACTGTCCTGACTCATTAGCCACATCCTTGCTAACTAAAAAACGGGCGGTAATACCACCGCCCGCTTGCTGAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAA  >  NC_000913/1181577‑1181868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: