| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,240,929 | T→C | 7.2% | T381T (ACA→ACG) | cvrA ← | putative cation/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,240,929 | 0 | T | C | 7.2% | 138.6 / 3.2 | 58 | T381T (ACA→ACG) | cvrA | putative cation/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (2/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01 | |||||||||||
CCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCT > NC_000913/1240781‑1241077 | ccGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGAt < 1:464257/149‑1 (MQ=255) aCGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTg < 1:609459/149‑1 (MQ=255) aCGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCGTTTTTAGCCGCCCACGAGAGTGATGTTCCCTg > 2:556373/1‑149 (MQ=255) gCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCa < 2:704679/149‑1 (MQ=255) cACCATTTATCGGCACTCACTTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCaa < 1:189417/149‑1 (MQ=255) aTTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGGGATGTTCCCTGCAAGAGc > 1:893751/1‑149 (MQ=255) aTTTATCGGCACTCAATTGATAAACAAACTGCTACCACGGATTTTCCGGCTGAATAACCAGGCAAACGCGCGACACCGGACGTCACACTGGCAGAACCACAACCTCGGATTTTTTCGCCGCACACGAAAGTAGTGTTCCCCGAATGAGc > 2:322261/1‑149 (MQ=255) tttATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCa < 1:556377/149‑1 (MQ=255) cGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTTATGTTCCCTGCAAGAGCGGTgga > 1:309889/1‑147 (MQ=255) cGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGaa > 1:175160/1‑149 (MQ=255) cAATTGATAAACAAACTGCTCTCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCCCTAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGaa < 2:445163/149‑1 (MQ=255) aaTTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAAc > 1:819456/1‑149 (MQ=255) tGATAAACAAACTGCTCCCACGGAGTTTCCGGATGAATACCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAAAATACGTTTGCCTGCAAGAGCAGTGAAACCAGAACcac < 2:250999/149‑1 (MQ=255) tAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTCGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACaaa < 1:770067/149‑1 (MQ=255) aaacaaacTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGACGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAaag > 2:855617/1‑149 (MQ=255) aaacaaacTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGACGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAaag > 2:855014/1‑149 (MQ=255) acaaacTGCTCCCCCGGATTTTCCGGATGAATATCCAGGCAAACGCTTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAaagaa < 1:215285/149‑1 (MQ=255) acTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACAGCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCg < 2:13392/149‑1 (MQ=255) tGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGAc < 1:111226/149‑1 (MQ=255) cTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACAt < 1:853740/149‑1 (MQ=255) tCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACAtt > 2:784335/1‑149 (MQ=255) ccACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTaa > 1:199117/1‑149 (MQ=255) cACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAACAAGGGCGACATTaaa > 2:247256/1‑149 (MQ=255) ggATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGaa < 2:199117/149‑1 (MQ=255) ttttCCGGATGAACATCCAGGCCAACGCGTGACCCCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGATTGATATTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAg < 1:387124/149‑1 (MQ=255) ccGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGt < 1:428331/149‑1 (MQ=255) cgATGAATATCCAGGCCAGCGCGTGACCCCGGACGTCCAACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGATGGAGGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGc < 2:452228/148‑1 (MQ=255) gAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAAAAGGCGACATTAAAGAACAGACGTGCATtc > 1:635043/1‑149 (MQ=255) gAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGCGATGTTCCCTGCAAGAGCAGTGGAACCAGAACCACAAAGAAAGCCACATGAAAGAACAGACGAGCAtcc > 2:240311/1‑147 (MQ=255) ccAGGCCAACGCGTGACACCGGACTTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGc > 1:855618/1‑149 (MQ=255) acgCCAACTCCTGACACCGGACGTCCCACTAGCGGAACCACCACCTTAGCTTTTTTAGCCGCCCACGAGAGCGATGTTCCCTGCAAGAGCACAGAAACCAGAACCCCAAAGAGCGCGACATTAAAGAACAGCCGCGCACACTCCAGccc > 2:154354/3‑149 (MQ=255) aGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGccc > 1:803253/1‑149 (MQ=255) ccAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCAGTCCCCAGCCCCGc > 1:192363/1‑149 (MQ=255) gTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGACCCACACACAAGGCGACATTAAAGAACAGACGTGCATCCTCCAGCCCCGCActcatc > 1:20852/1‑149 (MQ=255) tGACCCCGGACGTCCCACTGGCGGAGCCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAACCCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCg < 1:748472/149‑1 (MQ=255) tGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATCAAAGAACACACGTGCAATCTCCAGCCCCGCCaccatcc > 2:280209/1‑148 (MQ=255) acCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGACGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATGCTCCAGCCCCGCCATCATCGGGaa < 1:855014/149‑1 (MQ=255) acCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGACGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATCCTCCAGCCCCGCCATCATCGGGaa < 1:855617/149‑1 (MQ=255) ggACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAATGAAGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAacac > 1:793078/1‑149 (MQ=255) aCGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTACCCTGCAAGAGCAGTGAAACCCGAACCACAAAGAAGGCAACATTAAAGAACACACGAGCATCCTCCAGCCCCGCCATCATCCCGCACACgg > 2:968892/1‑149 (MQ=255) tCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCa < 1:371389/149‑1 (MQ=255) cccACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCGCGCAAGAGCAGCGAAACCAGAACCACAAAGAAGGCGACATGACAGAACAGACGCGCATTCTCCAGCCCCGCCATCATCGCGAGCACGGCCAg > 2:755184/1‑149 (MQ=255) ccACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAgg < 2:99012/149‑1 (MQ=255) ggCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGATCCCTGCAAGAGCAGTGAAACCAGACCCACAAAGAAGGCAACATTAAAAAACAGACAAGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGgatgag > 2:515020/1‑148 (MQ=255) ggAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTgg < 1:580959/149‑1 (MQ=255) aaCCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACCAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCa < 2:699511/149‑1 (MQ=255) aaCCACCACTTTCGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCa < 2:831489/149‑1 (MQ=255) accaccACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCAc > 1:1132423/1‑149 (MQ=255) caccacTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCACGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCAcgg > 1:762152/1‑147 (MQ=255) ttttttAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTCAAGAACAGACGTGCATGCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGACGATTGGCACCGCGACGCGTAATc > 2:362390/1‑149 (MQ=255) tttAGCCGCCCACGATAGTGATGTGCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTa < 1:240311/149‑1 (MQ=255) tAGCCGCCCACGGGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTAcc < 2:732357/149‑1 (MQ=255) tAGCCGCCCACAGGAGGGATGTTCCCTGCAAGTGCAGGGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTAcc < 2:650328/149‑1 (MQ=255) ccgccCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAg > 2:424463/1‑149 (MQ=255) gcgTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAaca > 2:142633/3‑149 (MQ=255) tGATGTTCCCGGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACAcgc < 1:247256/149‑1 (MQ=255) ggATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACAcgc < 1:197226/148‑1 (MQ=255) aTGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAACCACgcgc > 1:977335/1‑149 (MQ=255) aTGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACgcgc > 1:91065/1‑149 (MQ=255) tGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCt > 2:1145410/1‑149 (MQ=255) | CCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAAGAGCAGTGAAACCAGAACCACAAAGAAGGCGACATTAAAGAACAGACGTGCATTCTCCAGCCCCGCCATCATCGGGAACACGGCCAGGATGATTGGCACCGCGCCGCGTAATCCTACCCAGCTGATAAACACGCGCT > NC_000913/1240781‑1241077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |