Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,093,040	G→A	100%	A215T (GCT→ACT)	hisC →	histidinol‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,093,040	0	G	A	100.0%	201.8 / NA	66	A215T (GCT→ACT)	hisC	histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/33);  total (33/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAT  >  NC_000913/2092895‑2093182                                                                                                                                                  |                                                                                                                                               gggttttTGCACGCTGCTGGAGTTAACCCGCGGTAATGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTTCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtt                                                                                                                                             <  2:42213/146‑1 (MQ=255)     tttCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATCTTACGCACACTGTCGAAAACTGTTGc                                                                                                                                         >  1:961765/1‑149 (MQ=255)     ttgtgCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGc                                                                                                                                         <  1:234812/145‑1 (MQ=255)          cACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCGATTTTACGCACACTGTCGAAAACTTTTGCTCTgg                                                                                                                                    >  1:808385/1‑149 (MQ=255)             ccTGCTGGAGTTAACCCGCGGTAATGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                 >  1:858378/1‑149 (MQ=255)             ccTGCTGGAGGTCAGCCGGCGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                 <  2:775037/149‑1 (MQ=255)              ctgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCggg                                                                                                                                <  1:986064/149‑1 (MQ=255)                    gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGGTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCg                                                                                                                          >  2:413560/1‑149 (MQ=255)                      gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                        >  2:1126492/1‑149 (MQ=255)                        tAACCCGCGGTAGGCGGATTGTGGGTGTCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCGGGCGGAATGTCCGCACCTGGCTATTTTACGCACACTGTAGAAAACTTTTGCTCTCGCGGGGCTTCGTTGc                                                                                                                      <  1:82065/149‑1 (MQ=255)                            ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGGGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGGGGAATATCCGCCCCTGGCTATTTTACGCCCACTGTCGAAAAATTTTGCTCTGGCGGGGCGTGGTTGCgggt                                                                                                                  >  2:31754/1‑147 (MQ=255)                                ggTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                              <  2:47818/149‑1 (MQ=255)                                 gTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGTTTCGTTGCTGCGTTACg                                                                                                             >  2:80271/1‑149 (MQ=255)                                 gTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTCCGTTGCGGATTTACg                                                                                                             >  2:79608/1‑149 (MQ=255)                                   aaGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCCATTTTACGCACACTGACGAAAACTTTTGCTCTGGCGGCGCTTCGTTGCGGAGTTGCGCt                                                                                                           >  2:111456/1‑149 (MQ=255)                                      gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGc                                                                                                        >  2:686913/1‑149 (MQ=255)                                        gATTGTGTTTGCCGATGAAGCCTAGATCGAGTTTTGCCCGCAGGCATCGCGGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCaa                                                                                                      <  2:220336/149‑1 (MQ=255)                                                ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                              <  1:1044698/149‑1 (MQ=255)                                                   ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtc                                                                                           <  1:122786/149‑1 (MQ=255)                                                    cGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtca                                                                                          >  1:407001/1‑149 (MQ=255)                                                    cGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtca                                                                                          >  1:406619/1‑149 (MQ=255)                                                      aTGGAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAACATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGATTTTCGCTGGCAAACGAAGAAGtcatc                                                                                        <  1:641105/149‑1 (MQ=255)                                                      aTGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTTTCGAAAACTTTTGCTCTGGCGGGGCGTCGTTGCTGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                        <  1:748179/149‑1 (MQ=255)                                                         aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                     >  1:1142867/1‑149 (MQ=255)                                                              tatgTCGAGTTTTGCGCGCAGGCAGCGCTGGCTGGCTGGCTGGCGGAATCTCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgct                                                                                <  1:458786/149‑1 (MQ=255)                                                               atatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGGCGAAAACTTTGGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgctg                                                                               <  1:1089495/149‑1 (MQ=255)                                                                  tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCACACGAAGAAGGCAACAACCTGCtgatg                                                                            >  2:613012/1‑149 (MQ=255)                                                                  tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCCTCAACCTGCtgatg                                                                            >  1:403710/1‑149 (MQ=255)                                                                    gAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATAAACCTGCTGATGaa                                                                          >  2:1002060/1‑149 (MQ=255)                                                                     aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                         <  1:229228/149‑1 (MQ=255)                                                                     aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                         <  1:911312/149‑1 (MQ=255)                                                                     aGTTTGGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                         <  1:897856/149‑1 (MQ=255)                                                                      gTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAg                                                                        <  1:936782/149‑1 (MQ=255)                                                                       ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                       >  2:1105643/1‑149 (MQ=255)                                                                         ttGCCCGCAGGCATAGCTGGCTGGCTGGCTGGCGGACTATCCTCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTACGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGa                                                                     <  1:812239/149‑1 (MQ=255)                                                                          tGCCTGCAGGCATCGCTGGCTTGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGTTTCGTTGCGGATTTACGCTGGTAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGAt                                                                    <  1:422125/149‑1 (MQ=255)                                                                             ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAACGTGATCGc                                                                 >  1:978633/1‑149 (MQ=255)                                                                               gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                               <  2:846853/149‑1 (MQ=255)                                                                                cAGCCATCGCTGGCCGGATCGCTTTCTGGATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcccc                                                              <  1:495021/149‑1 (MQ=255)                                                                                 aGGCATCGCGGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGCGCTTCTGTGCGGATCTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccccc                                                             <  2:988793/149‑1 (MQ=255)                                                                                           ggctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGctct                                                   <  1:1105643/149‑1 (MQ=255)                                                                                              cggccggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                                <  1:298811/148‑1 (MQ=255)                                                                                               ggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAGCCGCCCTCGAc                                               >  2:375166/1‑149 (MQ=255)                                                                                                gctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACg                                              >  2:1072764/1‑149 (MQ=255)                                                                                                 ctggctggcGGAATATCCGCACCTGGCTATTTTCCGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                             <  2:208241/149‑1 (MQ=255)                                                                                                   ggctgccGGAATATCCGCACCTGGCTATTTTTCGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAATAAGTCAGCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                           <  2:8359/149‑1 (MQ=255)                                                                                                           gAATATCCCCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGa                                   <  1:1045560/149‑1 (MQ=255)                                                                                                               atCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATGAACCTGCTGATGAAAGTGATCGCCCCCTCCCCGCTCTCGACGCCGGTTGCCGACAta                               >  2:69257/1‑148 (MQ=255)                                                                                                                tCCGCACTTGGCTAGTTGACGCACAGTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGAAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTg                              <  1:364828/149‑1 (MQ=255)                                                                                                                   gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCgg                           >  1:189832/1‑149 (MQ=255)                                                                                                                   gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCgg                           >  2:190031/1‑149 (MQ=255)                                                                                                                   gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGATTCGTTGCGGATTTACGCTGGCACACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCAACGCCGGTTGCCGACATTGCgg                           >  2:492860/1‑149 (MQ=255)                                                                                                                    ccacTGGCTATTTTACGCGCACTGTCGAAAACTTTTGCGCTTGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGc                          <  1:287607/146‑1 (MQ=255)                                                                                                                     aCCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGcc                         >  2:617567/1‑149 (MQ=255)                                                                                                                       cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                       >  1:1076345/1‑149 (MQ=255)                                                                                                                       cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                       >  1:218963/1‑149 (MQ=255)                                                                                                                        tGGGTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAg                      <  1:290338/149‑1 (MQ=255)                                                                                                                            tATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                  >  1:293496/1‑149 (MQ=255)                                                                                                                            tATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                  >  2:362045/1‑149 (MQ=255)                                                                                                                                  aCGCACACTGTCGACAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTCGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGcc            <  1:203835/149‑1 (MQ=255)                                                                                                                                     cacaCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcac         <  2:1142867/149‑1 (MQ=255)                                                                                                                                      acacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca        <  2:293496/149‑1 (MQ=255)                                                                                                                                      acacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca        >  1:482993/1‑149 (MQ=255)                                                                                                                                       cacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAg       >  1:739415/1‑149 (MQ=255)                                                                                                                                            tCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCACACAGGGAAt  >  1:1046636/1‑149 (MQ=255)                                                                                                                                            tCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAt  >  1:814081/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                               GGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAT  >  NC_000913/2092895‑2093182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑