Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,589 T→G 7.1% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5890TG 7.1% 181.8 / 7.2 71intergenic (+73/‑32)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base T (29/37);  new base G (2/3);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

ACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTT  >  NC_000913/3156448‑3156733
                                                                                                                                             |                                                                                                                                                 
aCCCAACAGGGCGAAAATCATGAGATTACGTTGGATGTCAGCCGCCGTATATACGAGGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACCCCTCACCGGAGCCTGCTCCgg                                                                                                                                            >  2:785014/1‑149 (MQ=255)
  ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                          >  1:879822/1‑149 (MQ=255)
   ctacctggCGAAAATCATGACATTACGTTGGAGGTCAGGCGCCGTACATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACGTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCCCCGGAGTAGGCGCCGGTGa                                                                                                                                         <  2:139032/143‑1 (MQ=255)
   cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGa                                                                                                                                         <  2:865732/149‑1 (MQ=255)
       ggcgCGAAAAGCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTc                                                                                                                                     <  2:222563/146‑1 (MQ=255)
        gggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACTCACCGGAGCAGGCTCCGGTGAggtgtt                                                                                                                                   <  2:571996/149‑6 (MQ=255)
         ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACTCCTGCTCGCCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                   <  1:1141108/149‑1 (MQ=255)
           cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCTTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCagat                                                                                                                                 >  2:283902/1‑149 (MQ=255)
           cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATAAGAAACTGCCCGCTGAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCACTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCatat                                                                                                                                 >  2:759347/1‑149 (MQ=255)
            gAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                >  2:869303/1‑149 (MQ=255)
              ccataaTGACATTACGTAGCATTTCAGCCTCCGTATATACGAACCCTCCCTCTAATCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCTGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  2:510382/144‑1 (MQ=255)
              aaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                              <  2:639104/149‑1 (MQ=255)
                 tCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGCAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                           <  1:702605/149‑1 (MQ=255)
                 gcATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                           <  2:1133767/148‑1 (MQ=255)
                  cATGACATTACGTTGGATGTCAGCCTCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGGGCGTTAACATAAaggag                                                                                                                          >  1:580073/1‑149 (MQ=255)
                    tGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAgggaggc                                                                                                                        >  1:106828/1‑148 (MQ=255)
                     gACGTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGaa                                                                                                                       <  1:121587/149‑1 (MQ=255)
                        aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGt                                                                                                                    <  1:173858/149‑1 (MQ=255)
                            cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                                                                <  2:1075133/149‑1 (MQ=255)
                            cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATgg                                                                                                                <  1:691506/149‑1 (MQ=255)
                                  aTGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTATGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTaaac                                                                                                          >  2:898154/1‑147 (MQ=255)
                                   tGTCATCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCACACCTTCGTTTCCGGGCAGTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATcc                                                                                                         <  2:788330/149‑1 (MQ=255)
                                        gccgccGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                                                                    >  2:131942/1‑149 (MQ=255)
                                         ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGGCTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATAAAAAGGAGGAACGTATGGCTAATCCAACCGt                                                                                                   >  1:160750/1‑149 (MQ=255)
                                          cgccgTAGATACGAAGCCGCCCCCTAAGCCTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                  <  1:376614/149‑1 (MQ=255)
                                            ccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGGTTCATATAAGGGAGGAACGTATGGCTAATCCAACCGttat                                                                                                >  2:349112/1‑149 (MQ=255)
                                             cgtttttaCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatt                                                                                               <  2:62969/143‑1 (MQ=255)
                                              ttatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTGAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATCTCACGGAGGAACGTATGGCTAATCCAACCGtgatta                                                                                              <  2:105414/148‑1 (MQ=255)
                                               tatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttattca                                                                                             >  1:260162/1‑147 (MQ=255)
                                               tatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTATTCGGGCATTTCGTCCAGTCTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                             >  2:944597/1‑149 (MQ=255)
                                                 aataCGAAGCCGCCCGATTAGCTTTTTACGCCTACAACCTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                                                                           <  2:629740/148‑1 (MQ=255)
                                                  ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCt                                                                                          <  1:491506/149‑1 (MQ=255)
                                                     cGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                                                                       >  1:393579/1‑149 (MQ=255)
                                                          ccgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATg                                                                                  <  1:1075585/149‑1 (MQ=255)
                                                                    aGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCAt                                                                       <  2:852767/149‑1 (MQ=255)
                                                                     gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg                                                                      <  1:454219/149‑1 (MQ=255)
                                                                     gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCCCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg                                                                      <  2:774768/149‑1 (MQ=255)
                                                                       tttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCACTGTCATGcc                                                                    >  2:999792/1‑149 (MQ=255)
                                                                        ttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGccc                                                                   <  2:59931/149‑1 (MQ=255)
                                                                             cGCCTGAAACTTTCGTTTTCGGGCATTTCGTCTAGACTTAAGTGCACAACACCTCACCGGAGCCTGCTACGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATTTCATGCCCCAGCt                                                              <  1:785014/149‑1 (MQ=255)
                                                                              gCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                                                             <  1:708884/149‑1 (MQ=255)
                                                                               ccTCAAGCTTTCGTTTTCGGGCATTTCGCCCAGACCTAAGTTCACAACACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                            <  2:268622/149‑1 (MQ=255)
                                                                               ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                            <  2:114756/149‑1 (MQ=255)
                                                                                cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                           >  1:451108/1‑149 (MQ=255)
                                                                                 tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                          >  2:581806/1‑149 (MQ=255)
                                                                                 tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAAATAAGGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAAGTCATGCCCCAGCTGGGa                                                          >  1:326809/1‑149 (MQ=255)
                                                                                 tCAAACTTTCGGTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATACAGGAGGGACGTATGGCGAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                          <  2:418750/149‑1 (MQ=255)
                                                                                  cAAACTTTCGTTCTCGGGCATTTCGTCCAGACTTAAGTGCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTACGCTACAGGATGGCAATGTCATGCCCCAGCTgggcc                                                         >  1:166935/1‑147 (MQ=255)
                                                                                    aaCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCCTATACCGGAGGAACGCATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTg                                                       <  1:447457/149‑1 (MQ=255)
                                                                                     aCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCTCAGCTGGGACTgg                                                      <  1:785531/149‑1 (MQ=255)
                                                                                            ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCAAGCTGGGACTGGGCGTATg                                               >  1:459950/1‑149 (MQ=255)
                                                                                                    cATTTCGTCCAGACTTCAGATCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                       <  1:22696/149‑1 (MQ=255)
                                                                                                    cATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAACCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                       <  2:207438/149‑1 (MQ=255)
                                                                                                       ttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTa                                    <  1:1085175/149‑1 (MQ=255)
                                                                                                         cGTCCAGCCTTATGTTCACAACACCCCACCGGAGCCTGCTACGGTGAGTTCATATAAAGGAGGAACGTATCCGTCAATCGGTCATAATCGAGCGACAGAGTGATCGATGGATGCCCCAGCTAGGACTGGTCGGCTGGCAAGCAAGTAAt                                  <  2:245079/149‑1 (MQ=255)
                                                                                                           tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                <  1:1068018/149‑1 (MQ=255)
                                                                                                           cccagctttAGGTTCACACGACCTCACCGGACCCTGCCCCGGTGAGTTCCTATAGAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGACCGCAATGTCATGCCCCCGCTGGGACTGGGTGTCTGGCAAGCAAGTAATGa                                <  2:29013/142‑1 (MQ=255)
                                                                                                             cAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGGTGTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAgg                              >  1:847925/1‑149 (MQ=255)
                                                                                                              aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAt                                                                            <  1:330240/102‑1 (MQ=255)
                                                                                                              aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAt                                                                            >  2:330240/1‑102 (MQ=255)
                                                                                                              aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGGCTGGGCATCTGGCAAGCAAGTAAggagga                             >  1:757958/1‑149 (MQ=255)
                                                                                                                  ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTa                         >  1:721945/1‑149 (MQ=255)
                                                                                                                        tCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTGATATAAAGGAGGAACGTATCGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCCGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAcc                   >  1:413352/1‑149 (MQ=255)
                                                                                                                          acaacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAATAATCACCGc                 >  2:162747/1‑149 (MQ=255)
                                                                                                                             acacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAt              >  2:890044/1‑149 (MQ=255)
                                                                                                                                 cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa          >  1:664264/1‑149 (MQ=255)
                                                                                                                                 cTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa          >  1:664325/1‑149 (MQ=255)
                                                                                                                                  tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa         >  1:582828/1‑149 (MQ=255)
                                                                                                                                  tCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa         >  2:482996/1‑149 (MQ=255)
                                                                                                                                      cGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGACGTTATCACCGCCGTTCAAAAAGc     <  2:393579/149‑1 (MQ=255)
                                                                                                                                         aGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGtt  <  1:1151990/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
ACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTT  >  NC_000913/3156448‑3156733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: