Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,171 C→G 6.2% P116R (CCG→CGG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,1710CG 6.2% 189.0 / 3.6 66P116R (CCG→CGG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (26/34);  new base G (2/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGA  >  NC_000913/3368024‑3368311
                                                                                                                                                   |                                                                                                                                             
tCAGGCGTCTATCAAAGGATTTATGTCTAATGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTcca                                                                                                                                              <  2:911152/149‑2 (MQ=255)
tCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGTGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCg                                                                                                                                              <  2:632072/149‑1 (MQ=255)
  aaGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCa                                                                                                                                            <  1:19740/149‑1 (MQ=255)
   aGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGCAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAg                                                                                                                                           <  1:823383/149‑1 (MQ=255)
    gCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGa                                                                                                                                          <  2:1123152/149‑1 (MQ=255)
       aCTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGacac                                                                                                                                       <  1:19425/149‑1 (MQ=255)
          aTCAAAGGATGGATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACTCCATTGGGAATTCCGCAGACACCGt                                                                                                                                    <  1:596755/149‑1 (MQ=255)
             aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTg                                                                                                                                 >  1:870734/1‑149 (MQ=255)
             aaaGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTg                                                                                                                                 <  1:807868/149‑1 (MQ=255)
              aaGGATTTATGTCGAAGGTATATGCGTGGGTTATCTGCTAAAGACCTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAATACCATTGGGAATTCCGCAGACACCGTCTGc                                                                                                                                <  1:602198/149‑1 (MQ=255)
               aGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCa                                                                                                                               >  1:24318/1‑149 (MQ=255)
                 gATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGa                                                                                                                             >  2:1102050/1‑149 (MQ=255)
                  aTTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGaa                                                                                                                            <  1:1108175/149‑1 (MQ=255)
                    ttATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAtt                                                                                                                          <  2:661760/149‑1 (MQ=255)
                      aTGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCGGCAGACACCGTCTGCAGAATTcc                                                                                                                        >  1:122153/1‑149 (MQ=255)
                      aTGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCGGCAGACACCGTCTGCAGAATTcc                                                                                                                        >  1:720059/1‑149 (MQ=255)
                       tGTCTAAGGTATAAGCGTGCGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTGTTACCTCGTTTTTCAACATGTTGAAATGCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTccc                                                                                                                       <  2:111496/149‑1 (MQ=255)
                        gTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCa                                                                                                                      <  1:488754/149‑1 (MQ=255)
                         tCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGCAGACGGTGTCTGCGGAATTCCCaa                                                                                                                     <  1:1051315/149‑2 (MQ=39)
                          cTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGCATTAACACCATTGGGAATTCCGCAGACCCCGTCTGCAGAATTCCAATc                                                                                                                    >  1:753527/1‑149 (MQ=255)
                           tAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCg                                                                                                                   >  2:1062302/1‑149 (MQ=255)
                             aGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGc                                                                                                                 >  1:1019593/1‑149 (MQ=255)
                               gTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaa                                                                                                               <  1:374274/149‑1 (MQ=255)
                                tataAGGGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCGGCAGAATTCCCATCGGCaaa                                                                                                              >  1:1106163/1‑149 (MQ=255)
                                tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                                                                                              <  1:639928/149‑1 (MQ=255)
                                tataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGAAATTCCGCAGACACCGTCTACAGGATACCCACCGGCaaa                                                                                                              >  2:160064/1‑149 (MQ=255)
                                tataAGCGTGGGTTCTCTGCAAAAAAATTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                                                                                              <  1:110702/149‑1 (MQ=255)
                                 ataAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                                                                                             <  2:191021/149‑1 (MQ=255)
                                  taAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                                                                                            <  2:444732/149‑1 (MQ=255)
                                     gCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAttt                                                                                                         >  2:863393/1‑149 (MQ=255)
                                       gTGGGTTCTCTCCAAAAAACTTACCCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTgg                                                                                                       <  2:771365/149‑1 (MQ=255)
                                            ttCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAc                                                                                                  >  1:446037/1‑149 (MQ=255)
                                             tctcTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACg                                                                                                <  2:822473/149‑1 (MQ=255)
                                             tctcTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACg                                                                                                <  1:1062302/149‑1 (MQ=255)
                                               tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCt                                                                                              >  1:854723/1‑149 (MQ=255)
                                               tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCt                                                                                              >  2:762071/1‑149 (MQ=255)
                                                 tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACCCTGt                                                                                            >  1:64726/1‑149 (MQ=255)
                                                   cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                          <  2:489576/149‑1 (MQ=255)
                                                    aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                         >  1:1102849/1‑149 (MQ=255)
                                                    aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCACAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                         <  2:488231/149‑1 (MQ=255)
                                                       aaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTCGGCAGACGCTGTCTGCCa                                                                                      >  2:908074/1‑149 (MQ=255)
                                                         aCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCCGACACTGTCTGCCaaa                                                                                    >  1:544307/1‑149 (MQ=255)
                                                          cTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGCAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAt                                                                                   <  1:201376/149‑1 (MQ=255)
                                                            tACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCGCATCGGCAAAGATTTGGCGGGCGCTCTCTGCCGAATCt                                                                                 >  2:512895/1‑149 (MQ=255)
                                                               gCCAAATGAGGCTTTTTTACCTCTTTTTTCAAAATGTTGAAATTCACCAGACCATGTTTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATACGCAACGATTTCGCAGCCGCTGTCTGCCCAATCGTTc                                                                              >  2:552401/1‑149 (MQ=255)
                                                                cctattGAGGCTTTTTTACCTATTTTTTTAACAGGTTGAAATTCACCAGACTTTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                             <  2:185912/144‑1 (MQ=255)
                                                                ccAAATGAGTCTTTTTTACCTCTTTTTTCAACATGTTGAAAGTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCACAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                             <  1:735135/149‑1 (MQ=255)
                                                                ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCAAATCtttgc                                                                             <  2:24318/149‑3 (MQ=255)
                                                                           ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGGAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAgg                                                                  >  1:228097/1‑149 (MQ=255)
                                                                                tACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                             <  2:836338/149‑1 (MQ=255)
                                                                                    gtgaatttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCAAAATCTTTCCCCTTGCCCAGGTCCACCtcc                                                         >  2:71985/6‑147 (MQ=255)
                                                                                          ttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCTGTAGACACCGTCTGCAGAATTCCCATCGGCAACGATTTGGCAGACGCTGTCTGCCAAATCTTTCACCTTGCCCAGGTCCACCTACGTCCGt                                                   <  2:576250/149‑1 (MQ=255)
                                                                                            cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCGGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                 <  2:122153/149‑1 (MQ=255)
                                                                                            cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCGGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                 <  2:720059/149‑1 (MQ=255)
                                                                                             aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGAAACCGTCTGCAGAATTCCCATCGGCAAAGATTAGGCAGACGCTGTCTGCCAAATCCTTCCACTTGCCCAGGTCCACGTAAGTCCGTTTa                                                >  2:204951/1‑149 (MQ=255)
                                                                                              aCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCCGGTCCACCTACGTCCGTTTAc                                               >  2:488084/1‑149 (MQ=255)
                                                                                                  gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTc                                           <  1:1102050/149‑1 (MQ=255)
                                                                                                             ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCAATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTGTCCGGTAAAAACg                                >  1:435266/1‑149 (MQ=255)
                                                                                                                 aCAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                            >  2:278925/1‑149 (MQ=255)
                                                                                                                  cAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGAc                           >  2:22231/1‑149 (MQ=255)
                                                                                                                       tCTGCCGAATCAACACCATTGGCAAGCCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATCTGGCACACGCTGTCCGCCAAATCTTTCCCCTTGCCCAGGAACCCCTACGGCCCTTTACTTTCCGTTAAAAACGCTGACGCCCg                      <  2:483650/149‑1 (MQ=255)
                                                                                                                                       cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa      <  1:179082/149‑1 (MQ=255)
                                                                                                                                        aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag     >  1:1096448/1‑149 (MQ=255)
                                                                                                                                        aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag     <  2:463952/149‑1 (MQ=255)
                                                                                                                                         ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga    <  2:64726/149‑1 (MQ=255)
                                                                                                                                         ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga    <  2:228097/149‑1 (MQ=255)
                                                                                                                                           gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAgaga  <  1:762071/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGA  >  NC_000913/3368024‑3368311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: