Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 69 [0] [0] 69 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)67 (0.950) 56/278 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

TCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258543‑258831
                                                                                                                                             |                                                                                                                                                    
gttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                               <  1:723834‑M2/8‑1 (MQ=255)
 ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                              >  1:870149‑M2/141‑149 (MQ=255)
   aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgacgggctgcaccaggaaaaaatttaccgcactaggcccgtatattcgtgcaggtgTGTGCAAAGAt                                                                                                                                            >  1:599965‑M2/139‑149 (MQ=255)
       atttgctaaattgtgccaatgtggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                        <  2:96830‑M2/15‑1 (MQ=255)
        tttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAtca                                                                                                                                       >  1:722378‑M2/134‑148 (MQ=255)
          tgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                                                                     <  1:807896‑M2/18‑1 (MQ=255)
          tgcgaaatgttgccaatttggtaaaacagtggcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAt                                                                                                                                     <  2:178474‑M2/18‑1 (MQ=255)
           gctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                                                                    <  2:971075‑M2/19‑1 (MQ=255)
             taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacacgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAACCGATTCt                                                                                                                                  >  2:237784‑M2/129‑149 (MQ=255)
                        aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                       >  1:87091‑M2/118‑149 (MQ=255)
                         atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                      <  1:644572‑M2/33‑1 (MQ=255)
                          tttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt                                                                                                                     <  1:1231529‑M2/34‑1 (MQ=255)
                             ggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCACAGATGATGGATGCTTTTTCGATTGTCTGGc                                                                                                                  >  1:25507‑M2/113‑149 (MQ=255)
                              gtaaaacagttgcatcacaacaggatatgccaaggacgccaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                 <  1:783429‑M2/38‑1 (MQ=255)
                               taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCATATTCGATTGTCTGGCTg                                                                                                                >  1:1183360‑M2/111‑149 (MQ=255)
                                 aaaccgttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                              <  1:304533‑M2/41‑1 (MQ=255)
                                 aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                              >  2:769602‑M2/109‑149 (MQ=255)
                                     agttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                                                                          <  2:923991‑M2/45‑1 (MQ=255)
                                      gttgcatcacaacaggggatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcattaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                         <  2:416192‑M2/46‑1 (MQ=255)
                                                  caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                             <  2:1233034‑M2/58‑1 (MQ=255)
                                                    ggagatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                           <  1:364349‑M2/60‑1 (MQ=255)
                                                     gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                                                                          >  1:555779‑M2/89‑149 (MQ=255)
                                                     gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGGATGCGTCGACGTGAAACcgga                                                                                          >  1:1030702‑M2/89‑148 (MQ=255)
                                                       gatagcaatgacgttaccgagtggacagccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTGTTCGCGTGTCTGGCGGTGTGCGTCAACGTGAAACCGGGAc                                                                                       >  2:649398‑M2/87‑149 (MQ=255)
                                                        atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTATCTGGCTGTATGCGTCAACGTGAAACCGGCAcc                                                                                      >  1:957551‑M2/86‑149 (MQ=255)
                                                                   gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCGGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                           >  1:266451‑M2/75‑149 (MQ=255)
                                                                     taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGTAAGGTGAAACCGGCACCGGCAGTGCGTGaa                                                                         >  2:169264‑M2/73‑149 (MQ=255)
                                                                        cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                      <  2:510604‑M2/80‑1 (MQ=255)
                                                                                 acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                             >  1:280662‑M2/61‑149 (MQ=255)
                                                                                  cccgaagagcagattgatcaaaaaattttccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                            <  1:53268‑M2/90‑1 (MQ=255)
                                                                                   ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                           <  1:1101154‑M2/91‑1 (MQ=255)
                                                                                    cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaCGTGCAAAAATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGAGCGTGAATTCGGGGGCtggtgg                                                          >  1:420255‑M2/58‑149 (MQ=255)
                                                                                     gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCCGGtgga                                                         >  2:144156‑M2/57‑149 (MQ=255)
                                                                                        gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                                      >  2:836187‑M2/54‑149 (MQ=255)
                                                                                        gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                                      <  1:881113‑M2/96‑1 (MQ=255)
                                                                                          gtcgattgatcaaaacatttcccggtctaggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCCGTATGCGTCAACGTGAAACCGGCACCCGTGAATGGTGAATTCTGGGGCTGGTGGATGGAg                                                    <  2:582682‑M2/98‑1 (MQ=255)
                                                                                             gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAGTCTGGGGCTGGTGGATGGAGCTg                                                 <  1:673313‑M2/101‑1 (MQ=255)
                                                                                              attgatcaaaacactttccgcactcggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGACTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                <  1:228977‑M2/102‑1 (MQ=255)
                                                                                              attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGACTTCTGGGGCTGGTGGATGGAGGTgg                                                >  2:395275‑M2/48‑149 (MQ=255)
                                                                                                tggtcaaaaaattcaccgcactagggccgtatattagggaaggtgAGTGGGAAGATGAGCGGTTCTTTGTCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGGACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                              <  2:443105‑M2/104‑1 (MQ=255)
                                                                                                tgatcaaaaaatttcccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCAGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                              <  2:790720‑M2/104‑1 (MQ=255)
                                                                                                tgagcaaaaaatttgccgcacaaggcccgtataatgataaagttaAGGGCAAAGAGTATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                              <  2:442606‑M2/104‑1 (MQ=255)
                                                                                                  atcaaaaaatttaccgcactaggcccgtataatagagacgggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTGAACGTGAAACAGGCACCGGACGTGCGTGAATCCTGGGGCTGGTGGATGGAGCTGGAAgc                                            >  1:300432‑M2/44‑149 (MQ=255)
                                                                                                   tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                           >  2:535288‑M2/43‑149 (MQ=255)
                                                                                                   acaagaaagttaccgcacgaggcccgtatagtcgtgaaggtaAGTGCAAAGATAATCGATGCTTTGTCGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCCCCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                           <  2:590178‑M2/107‑1 (MQ=255)
                                                                                                    ctaaaaagttaccgcactaggcccgtatattcgtgaaggtaCGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGGGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcgc                                          <  2:603985‑M2/108‑1 (MQ=255)
                                                                                                     aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                         >  1:1236536‑M2/41‑149 (MQ=255)
                                                                                                      aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                        >  1:192064‑M2/40‑149 (MQ=255)
                                                                                                      aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTAGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGGAGTGCGTGAATTCTGGGGCGGGTGGATGGGGGtggaaggggag                                        >  2:241858‑M2/40‑144 (MQ=255)
                                                                                                       aaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCCGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAgg                                       >  2:692123‑M2/39‑149 (MQ=255)
                                                                                                          atttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGAGGGAGCTGGAAGCGCCGGGAt                                    >  2:541565‑M2/36‑149 (MQ=255)
                                                                                                              accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGCTGGCGCTGGAAGCGCCGGAATCCCg                                >  2:846370‑M2/32‑149 (MQ=255)
                                                                                                               ccgcactgggcccgtatatttgtgaagggaAGTGCAAAGAGAATCGATTTTGTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                               <  1:49831‑M2/119‑1 (MQ=255)
                                                                                                               ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                               <  1:357348‑M2/119‑1 (MQ=255)
                                                                                                                 gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGGGAATTCTGGGGCTGGCGGGTGGAGCTGGACGCGCAGGAATCCCGgtt                             >  1:728931‑M2/29‑149 (MQ=255)
                                                                                                                  cattaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGGAGCTGGGCGCGCAGGGATCCCGtttt                            >  2:530354‑M2/28‑149 (MQ=255)
                                                                                                                   actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTa                           <  2:643241‑M2/123‑1 (MQ=255)
                                                                                                                    ctaggcccgtatattcgtgaaggtaAGGGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                          <  2:592678‑M2/124‑1 (MQ=255)
                                                                                                                       ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTATATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                       <  1:464311‑M2/127‑1 (MQ=255)
                                                                                                                          ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                    >  1:650600‑M2/20‑149 (MQ=255)
                                                                                                                          ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                    <  1:1194713‑M2/130‑1 (MQ=255)
                                                                                                                           cgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt                   >  1:81989‑M2/19‑149 (MQ=255)
                                                                                                                           actatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGACTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt                   <  1:411729‑M2/131‑1 (MQ=255)
                                                                                                                              atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCTGTATGAGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGTAAGCTCAGGAATCCCATATTACATACAGTTAc                >  2:1128356‑M2/16‑149 (MQ=255)
                                                                                                                                attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa              <  2:87091‑M2/136‑1 (MQ=255)
                                                                                                                                attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTCACCa              <  1:431784‑M2/136‑1 (MQ=255)
                                                                                                                                attcgtgaagggaAGTGCAAAGAGTATTTGTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa              <  1:839489‑M2/136‑1 (MQ=255)
                                                                                                                                     tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg         <  1:1235245‑M2/141‑1 (MQ=255)
                                                                                                                                            aAGTGCCAAGATAATGGATTCTTTTTCGATTGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  <  2:104516‑M2/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258543‑258831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: