Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,214,6460TG20.2% 173.4 / 10.1 84S534S (TCT→TCG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (38/29);  new base G (0/17);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 1.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  NC_000913/3214498‑3214794
                                                                                                                                                    |                                                                                                                                                    
aaCGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCt                                                                                                                                                      >  1:54392/1‑149 (MQ=255)
aaCGGAGGCTGATGGCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCt                                                                                                                                                      <  2:518009/149‑1 (MQ=255)
 aCGTATGCTGATGCCAGAAGACAAGATCCGCCAAGTGCTGAAGCTCGCCAAAGGGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg                                                                                                                                                     <  2:155323/149‑1 (MQ=255)
 aCGTACGCTGATAAGGGAAGACAAGATCCGCAAAATGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGCTTCTg                                                                                                                                                     >  2:733947/1‑149 (MQ=255)
  cGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGCGCTGCCGCTGGATTCTGc                                                                                                                                                    >  1:956299/1‑149 (MQ=255)
  cGTAGGCGGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAACGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGc                                                                                                                                                    <  1:39724/149‑1 (MQ=255)
   gTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAACCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCg                                                                                                                                                   <  1:643312/149‑1 (MQ=255)
    tATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAACAGTCAATCTCCATGGAAACGCCGATCGGTGATCATGAAGATTCGCATCTGGGGGATTTCATCGATGATACCACCCTCGAGCTGCCGCTGGATTCTGCGa                                                                                                                                                  <  2:424476/149‑1 (MQ=255)
     aTGCTGATGCCGGAAGACAAGATCCGGAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTAATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGac                                                                                                                                                 >  1:796929/1‑149 (MQ=255)
        cTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCGGGATTCTGCGaccac                                                                                                                                              >  1:126217/1‑149 (MQ=255)
         tGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccacc                                                                                                                                             >  1:217656/1‑149 (MQ=255)
         tGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGaccacc                                                                                                                                             <  1:486668/149‑1 (MQ=255)
               cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAACGc                                                                                                                                       >  1:632104/1‑149 (MQ=255)
               cGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGACAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGc                                                                                                                                       >  1:893893/1‑149 (MQ=255)
                   aGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCAGCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGGAAGCCtgc                                                                                                                                   >  1:69298/1‑149 (MQ=255)
                     aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgt                                                                                                                                 >  1:772551/1‑149 (MQ=255)
                      cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCtgcgtg                                                                                                                                >  2:1239469/1‑149 (MQ=255)
                         gATCCGCACAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                             <  1:1107942/149‑1 (MQ=255)
                          aTCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCGCCCTCGGGCGGCCGCTGGATGCTGCGACCACCGAAAGCCTGCGTGCGGc                                                                                                                            >  1:959999/1‑149 (MQ=255)
                            ccGCAAAGTGCTGAAGATCGCCAAAAAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCaa                                                                                                                          >  1:628276/1‑149 (MQ=255)
                             cGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCTTGCGTGCGGCAAc                                                                                                                         <  1:852428/149‑1 (MQ=255)
                             cGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAAc                                                                                                                         >  1:795934/1‑149 (MQ=255)
                                    tGCTGAAGAACGCCAAAGCGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCAACTAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCacgac                                                                                                                  <  1:174764/149‑1 (MQ=255)
                                     gCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCacgacg                                                                                                                 >  1:766763/1‑149 (MQ=255)
                                       tGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGGTTCTGCGACCACCGGAAGCCTGCGGGCGGCCCGGCGCGACGTg                                                                                                               >  2:801478/1‑149 (MQ=255)
                                         aaGAGCGCCAACGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGTTTCGATTCTGGGGGATTTCATCGAGGATACCACCCTTTTTCTTTCTAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgct                                                                                                             <  2:530992/149‑1 (MQ=255)
                                          aGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                            <  1:622562/149‑1 (MQ=255)
                                           gATCGCCAAAGAGCCAATCGCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGAGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctgg                                                                                                           <  1:842589/149‑1 (MQ=255)
                                              cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGGGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCCCGACCTgcgggctg                                                                                                        >  2:53311/1‑149 (MQ=255)
                                               tccAAAGAGCCAATCTCGATGGAAGCGCCGATGGGTGGTGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGATACCACCCTTTTTTTTTTTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGctggctgg                                                                                                       <  2:323767/148‑1 (MQ=255)
                                                ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                        <  2:199548/147‑1 (MQ=255)
                                                ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                        >  1:199548/1‑147 (MQ=255)
                                                ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCtggctggc                                                                                                      <  1:1145135/149‑1 (MQ=255)
                                                ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCAGCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggctgac                                                                                                      >  1:316010/1‑147 (MQ=255)
                                                  aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGCCGTGCTGGCTGGCCt                                                                                                    >  1:763464/1‑149 (MQ=255)
                                                      cgCCAATCTCCATGGAAACGGCGATCGGTGATGATGAAGACTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTGTGTTTTTTAATGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGAcc                                                                                                <  2:1084660/148‑1 (MQ=255)
                                                      agCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCCCGACGTCCTGGCTGGCCGGAcc                                                                                                >  2:643131/1‑149 (MQ=255)
                                                       gCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcg                                                                                               >  1:529068/1‑149 (MQ=255)
                                                        ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGCGCTGCCGCTGGGTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgc                                                                                              >  1:237574/1‑149 (MQ=255)
                                                         cAATCTCCATGGAAACGCCGATCGGTGATGATGAGGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCCGGCCTGAGcggg                                                                                             >  2:1161272/1‑147 (MQ=255)
                                                          aacctcCAGGGAAGCGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTTCTGTCTATTGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcgc                                                                                            <  2:54392/146‑1 (MQ=255)
                                                                 aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGc                                                                                     >  1:503670/1‑149 (MQ=255)
                                                                          tcggtCGGTGAGTATGAAGATTCGCATCTGGGGGTGTTCATCGGGGATACCACCCTTGTGCTTTTGCAGGATACTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  2:934831/145‑1 (MQ=255)
                                                                          ccGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATTGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCt                                                                            <  1:630028/149‑1 (MQ=255)
                                                                            gATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTCCGTGCGGCAACGCGCGACGCGCGGGCTGGCCTGACCCAGCGTGAAGCATAAGATCAGc                                                                          >  2:1020719/1‑149 (MQ=255)
                                                                             ggcGGTTATGATGAAGTTTCGCATCTGGGGGATGTCGTCGAGGATACCACCCTCGTGTTGTCTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCg                                                                         <  2:1177399/147‑1 (MQ=255)
                                                                               cGGTGATGTGGAAGCTTCGCCTCTGGGGGATTTCATCGAGGATACCACCCTCGTTTTTCCGAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTa                                                                       <  2:1087205/149‑1 (MQ=255)
                                                                               cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGAGCGGCAACGCACGACGTGCTGGCTCGCCTGACCGCGCGCGAAGACAAAGTTCTGCgga                                                                       >  2:681423/1‑147 (MQ=255)
                                                                                  tgatgatgaCGACTCGCATCTGGGTGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTGCTGCGTATGc                                                                    <  1:1015474/149‑1 (MQ=255)
                                                                                  tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGACAGGCCTGACCGCGCGTGAGGCAAAAGTTCAGCGCATGc                                                                    >  2:644911/1‑149 (MQ=255)
                                                                                    atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                  >  1:1187179/1‑149 (MQ=255)
                                                                                     tgatgaAGATTCGTATCTGTGGGATTTCATCGCGGATCCCACCCTTGTTTTTTTTCATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGtt                                                                 <  2:99343/149‑1 (MQ=255)
                                                                                       atgaAGGTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                               <  2:842581/149‑1 (MQ=255)
                                                                                       atgaAGATTCGTATCTGGGGGATTTCATCGAGGTTACCACCTTTTTGCTTTTGATGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                               <  2:230500/149‑1 (MQ=255)
                                                                                       atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTc                                                               >  2:206796/1‑149 (MQ=255)
                                                                                        tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGGCCGCGCGTGAAGCAAAAGTTCGTCGGATGCGGTTCg                                                              >  2:318392/1‑149 (MQ=255)
                                                                                            gtgtCGCATTGGGGGGCTTTCATCGAGGATACCCCCCTCGTGCTGCCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTAt                                                          <  2:796929/146‑1 (MQ=255)
                                                                                             aTTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATc                                                         <  2:503670/149‑1 (MQ=255)
                                                                                                cGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                      <  1:1187607/149‑1 (MQ=255)
                                                                                                cGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                      <  1:1189308/149‑1 (MQ=255)
                                                                                                     cTGGGGGATTTCATCGAGGATACCACCCTCGTGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGaa                                                 <  2:956638/149‑1 (MQ=255)
                                                                                                        ggggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                              <  1:1239469/149‑1 (MQ=255)
                                                                                                        ggaggtttCATCGACCATAACACCCCCGACCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                              <  1:594107/144‑1 (MQ=255)
                                                                                                           gATTTCATCGAGGATACCACCCTCGAGGTGCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGa                                           <  2:217656/149‑1 (MQ=255)
                                                                                                             tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACCCCGACt                                         >  1:722576/1‑149 (MQ=255)
                                                                                                             tttCATCGAGGATACCACCCTCGAGCTGCCGCTAGATTCTGCGACCACCGAAAACCTGCGTTCGGCAACTCACGACCTGCTAGCTGCCCGGACCACGCGAGAAACAAAAGGTCTGCGGATGCGTTGCGGTATGGATCTGCACACCgcag                                         >  2:412871/1‑146 (MQ=255)
                                                                                                               tCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTac                                       <  2:795934/149‑1 (MQ=255)
                                                                                                                cATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTaca                                      <  1:943686/149‑1 (MQ=255)
                                                                                                                  tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACtcaccg                                    >  1:587457/1‑144 (MQ=255)
                                                                                                                  tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGCATGCGATGCGCTATCGGTATAGACACCGACGAGACg                                    >  2:933042/1‑149 (MQ=255)
                                                                                                                  tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGAACGCGCGTGAAGCAGAAGATCAGCGTATGCGGTTCAGTAGCGATCGGAACAGCCACAACACg                                    >  2:1093506/1‑149 (MQ=255)
                                                                                                                   cGAGGACCCCACCCTTGTGCTGCTGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGc                                   <  2:993105/149‑1 (MQ=255)
                                                                                                                        aTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGgaa                              <  1:206796/149‑1 (MQ=255)
                                                                                                                            caccCTCGCGCTGTCGCTGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCCGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGaagaag                          <  2:632104/149‑1 (MQ=255)
                                                                                                                              cccTCGATCTGCCGCTTGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg                        <  2:929274/149‑1 (MQ=255)
                                                                                                                               ccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                       >  2:1022589/1‑149 (MQ=255)
                                                                                                                                  cgtgtttccGATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTa                    <  2:158254/142‑1 (MQ=255)
                                                                                                                                   gtgCTGCCTCATGAGTCGGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaa                   <  2:772551/147‑1 (MQ=255)
                                                                                                                                    aGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGCCGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                  <  1:392519/149‑1 (MQ=255)
                                                                                                                                         ccGCGGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt             <  2:189971/149‑1 (MQ=255)
                                                                                                                                          cgcgGGGTTCGGCAACCACCGAAAGCGTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc            <  2:286892/149‑1 (MQ=255)
                                                                                                                                          cgatGGATTCGGCGACCACCGAAAGCCTGAGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc            <  2:915767/146‑1 (MQ=255)
                                                                                                                                            gtGGGTTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTGCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGa          <  1:437175/148‑1 (MQ=255)
                                                                                                                                            cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGa          >  1:655014/1‑149 (MQ=255)
                                                                                                                                                 ttCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCGGGCCCGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCGGGAACAGGTGCGTAAACCGTTCGACGTTa     >  2:206159/1‑149 (MQ=255)
                                                                                                                                                 tccTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGGGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa     <  1:333903/147‑1 (MQ=255)
                                                                                                                                                    tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTAccc  >  2:445832/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCC  >  NC_000913/3214498‑3214794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑