Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 47 [0] [0] 47 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)41 (0.990) 31/260 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258537‑258831
                                                                                                                                                   |                                                                                                                                                   
gcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                                                                    >  1:517084‑M2/148‑149 (MQ=255)
 cttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                                   <  1:457680‑M2/3‑1 (MQ=255)
 cttgtgttaaaaatttgctaaattttgccaatttgggataacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                                   >  2:169463‑M2/147‑149 (MQ=255)
         aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                           >  2:25000‑M2/139‑149 (MQ=255)
           aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                         >  2:45740‑M2/137‑149 (MQ=255)
                    aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                                <  1:365682‑M2/22‑1 (MQ=255)
                     aattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttt                                                                                                                               >  1:532817‑M2/127‑149 (MQ=255)
                          tgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                          >  1:297360‑M2/122‑149 (MQ=255)
                            ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggctcgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                        <  1:43698‑M2/30‑1 (MQ=255)
                             caatttggtaaaacagttgcatcagaacaggagatagcaatgacgttcccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactgggcccggatattcgtgaaggtaAGTGCAAAGCAAATCGATCCTTATTCGATTg                                                                                                                       >  2:705036‑M2/119‑149 (MQ=255)
                                  tggtaaaacagttgcaacacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                  <  2:331744‑M2/36‑1 (MQ=255)
                                           agctgcaacgcaacaggagatagcaatgacgttaccgagtggacccccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcggtaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                                                                         <  2:417285‑M2/45‑1 (MQ=255)
                                               gcatcacaacaggagatagcaatgacgttaccgagtggacacacgaagagcagattgatcaaaaaatttaccgcactaggaccgtagatgcgtgagggtaAGTGCAAAGCTAATAGATTCTTTTTGGGTTGTCTGGCTTTTTGCGTcag                                                                                                     >  1:91953‑M2/101‑148 (MQ=255)
                                               gcatcacaaaaggagatagcaatgacgttaacgcgtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATAGGTTCTTTTTGGATTGTCTGGCTGTATGCGTCaa                                                                                                     >  2:549945‑M2/101‑149 (MQ=255)
                                                   cacaacaggagatagcaatgacgttaccgagtggacacccggagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                                 >  2:459643‑M2/97‑149 (MQ=255)
                                                         aggcgttagcaaggaagttaccgggtggacacccgaagagcagattgataaaaaaattttccgcacttggcccgtatattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                                                                           <  2:124952‑M2/59‑1 (MQ=255)
                                                                     tgacgttaccgagtggccatcctaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTAAACGTGAAACCGGCACCGGAAGTg                                                                               <  1:580129‑M2/71‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGTACCGGAAGTGCGt                                                                            <  1:536567‑M2/74‑1 (MQ=255)
                                                                            accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                                                                        >  2:633821‑M2/72‑149 (MQ=255)
                                                                            accgaggggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATAGATTCTATTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTgaag                                                                        >  2:230096‑M2/72‑148 (MQ=255)
                                                                               gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGAAAGCGGAAGTGCGTGAATTCt                                                                     >  1:59849‑M2/69‑149 (MQ=255)
                                                                                    gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTATTGTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc                                                                >  2:524253‑M2/64‑149 (MQ=255)
                                                                                       acccgaagagcagattgaacaaaaaatctaccgcactgtgtccgtacaattgtgaatggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                             <  1:492591‑M2/89‑1 (MQ=255)
                                                                                         ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGGGTGAATTCTGGGGCgggtg                                                           >  1:19904‑M2/59‑149 (MQ=255)
                                                                                          cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACACCGGCACCGGAAGTGCGTGACTTCTGGGGCtggtgg                                                          >  2:84508‑M2/58‑149 (MQ=255)
                                                                                          cgaagagcagattgatcaaaaaatttaccgcactaggcccgtataacagtgaagggaAGTGCGAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGGGTGAATTCGGGGGCtggtgg                                                          >  2:515740‑M2/58‑149 (MQ=255)
                                                                                           gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                         <  1:169463‑M2/93‑1 (MQ=255)
                                                                                           gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                         <  2:526203‑M2/93‑1 (MQ=255)
                                                                                           gaagagcagatggatgaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                         <  2:600910‑M2/93‑1 (MQ=255)
                                                                                             agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCAGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                       <  1:676982‑M2/95‑1 (MQ=255)
                                                                                                     ctgatcaaaaaagttaccgcaataggcccgtagattcgtgagggtaAGTGCAAAGATAATCGATTCTTATTCGATTGTCTGGATGTATGCGTCAACGTGAAACTGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                               <  1:671633‑M2/103‑1 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                         >  1:621878‑M2/41‑149 (MQ=255)
                                                                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                        >  2:91972‑M2/40‑149 (MQ=255)
                                                                                                            aaaaatctaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGGGCTGGAAGCGCAg                                        >  2:681018‑M2/40‑149 (MQ=255)
                                                                                                                  ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATcc                                  <  1:459643‑M2/116‑1 (MQ=255)
                                                                                                                           taggcccgtatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAcc                         >  1:490672‑M2/25‑149 (MQ=255)
                                                                                                                            aggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCt                        >  1:314206‑M2/24‑149 (MQ=255)
                                                                                                                              gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCAGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGCAAGCGCAGGAATCCCGTTTTACCTAc                      <  2:397830‑M2/128‑1 (MQ=255)
                                                                                                                               cccgtattgtcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCCTCAACGTGGAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGATGCGCAGGAATCCCGTTTTACCTACa                     <  2:7098‑M2/129‑1 (MQ=255)
                                                                                                                               cccgtatattcctgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                     <  2:91953‑M2/129‑1 (MQ=255)
                                                                                                                               cccggatattcgggaatgtaAGTGAAACGTTAGTGGATTCGGTTGCGATTGTCTGGCTGCATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                     <  1:549945‑M2/129‑1 (MQ=255)
                                                                                                                                ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGACTTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                    >  1:574703‑M2/20‑149 (MQ=255)
                                                                                                                                ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                    >  2:61776‑M2/20‑149 (MQ=255)
                                                                                                                                ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                    >  1:356328‑M2/20‑149 (MQ=255)
                                                                                                                                         cgttaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCGCCGGAAGTGCGTGAATTCTGGGGCTGGTGGAGGGCGCGGGACGCGGCGGAATCCCGTTTTACCTACAGTTACCAGtt           >  2:689885‑M2/11‑149 (MQ=255)
                                                                                                                                                  aAGTGCAACGATAACCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  <  2:450610‑M2/148‑1 (MQ=255)
                                                                                                                                                  aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt  >  1:761036‑M2/2‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTT  >  NC_000913/258537‑258831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.