Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 732,825 C→T 7.9% S1081S (TCC→TCT rhsC → Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913732,8250CT 7.9% 108.5 / 3.0 51S1081S (TCC→TCTrhsCRhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (29/17);  new base T (2/2);  total (32/19)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

CGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAA  >  NC_000913/732699‑732973
                                                                                                                              |                                                                                                                                                    
cGACAGACCCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAACCGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAgtg                                                                                                                                <  2:255298/149‑1 (MQ=17)
 gACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCGCCAGCCCGGTGGCGAAGACGGTGGCAgtgt                                                                                                                               >  1:726094/1‑149 (MQ=12)
 gACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAgtga                                                                                                                               >  1:595293/1‑148 (MQ=17)
         ccGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTcc                                                                                                                       >  1:426630/1‑149 (MQ=32)
         ccGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCAACAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGAGCCGCAGCCTGGCGGATACCCTTCAGCAGGCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTcc                                                                                                                       >  2:566010/1‑149 (MQ=12)
          cGCATCCAGATGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTccc                                                                                                                      <  1:651602/149‑1 (MQ=17)
                 ggACGATTTATCAGCCGGGGAGCTTCACGCCGCTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTg                                                                                                               >  2:572307/2‑149 (MQ=17)
                  gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAATACGGTGGCAGTGTGGTGTTCCCGCCGGTGc                                                                                                              >  2:454729/1‑149 (MQ=17)
                  gACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGc                                                                                                              >  1:98026/1‑149 (MQ=32)
                            cAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGAt                                                                                                    >  2:195821/1‑149 (MQ=32)
                            cAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGGGTGGTGTTCCCGCCGGTGCTGGTGCGGAt                                                                                                    >  1:197539/1‑149 (MQ=17)
                            cAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTAGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGCTGTTCCCGCCAGTGCTGGTGCAGAt                                                                                                    >  1:476422/1‑149 (MQ=255)
                             aGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATg                                                                                                   >  2:87840/1‑149 (MQ=32)
                              gCCGGGGGGCTTCACGCCACGCAGCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGc                                                                                                  <  2:60893/149‑1 (MQ=12)
                                 ggggAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGGGTTCCCCCCGGGGCTGGTGCAGATGCCCg                                                                                               >  2:380441/1‑149 (MQ=255)
                                  gggAGCTTCACGCCACTCATCAGGGTTGAAACCGCCGCCGGTGAGCTGGCGAAAGCGCAGCGGGGCGGCCTGGCGGATACGCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGGGTTCCTGCCGGTGCTGGTGCGGGTGCCCGa                                                                                              >  2:774399/1‑149 (MQ=11)
                                  gggAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCTGCCGGTGCTGGTGCAGATGCTCGa                                                                                              >  1:723188/1‑149 (MQ=17)
                                       cTTCACGCCACTCAGCCGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGc                                                                                         <  1:187357/149‑1 (MQ=17)
                                         tCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAACCGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGTTGGTGCAGATGCTCGACCGGCTg                                                                                       <  1:412009/149‑1 (MQ=17)
                                         tCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTg                                                                                       <  1:236721/149‑1 (MQ=32)
                                           ccGCCACGCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGa                                                                                     >  2:485222/2‑149 (MQ=17)
                                           aCGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGa                                                                                     >  2:458819/1‑149 (MQ=32)
                                             gCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGaaa                                                                                   >  2:725638/1‑149 (MQ=32)
                                             gCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGCTGCTGGTCCAGATGCACGACCGGCTGGaaa                                                                                   >  1:207987/1‑149 (MQ=12)
                                                tcTCATCAGGGTTACAACCGCCACCGTCGAGCTGGCGAAACCGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGTCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                                <  2:412305/148‑1 (MQ=11)
                                                aCTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                                <  1:33485/149‑1 (MQ=32)
                                                aCTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTg                                                                                <  1:285996/149‑1 (MQ=32)
                                                  tcatcaGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGaa                                                                              >  1:770637/1‑149 (MQ=32)
                                                      caGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATcc                                                                          >  1:430374/1‑149 (MQ=32)
                                                           ttGACACCGCCCGCGGTCATGTGGCCAAAGCGCTGCGGCGCAGCCTGGCGGGTATCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                                     <  2:532943/149‑1 (MQ=11)
                                                           ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                                     <  2:56418/149‑1 (MQ=32)
                                                           ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                                     <  2:568673/149‑1 (MQ=32)
                                                           ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                                     >  1:594674/1‑149 (MQ=32)
                                                           ttGAAACCGCCACCGGGGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                                     <  2:369680/149‑1 (MQ=17)
                                                                aCCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGCCCg                                                                >  1:107237/1‑149 (MQ=17)
                                                                 ccGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAATACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                               <  1:454729/149‑1 (MQ=17)
                                                                 ccGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                               <  1:172190/149‑1 (MQ=32)
                                                                             aGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCTGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGaa                                                   >  2:29130/1‑149 (MQ=17)
                                                                             aGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCTGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGaa                                                   >  2:28781/1‑149 (MQ=17)
                                                                                tGGCGAAAACGCAACGCCGCAGCCTGGCGGATACCCGTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGGGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgc                                                >  1:535855/1‑149 (MQ=12)
                                                                                  gCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAACgccg                                              >  1:640317/1‑149 (MQ=17)
                                                                                    gAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGATCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAgccgcc                                            >  2:565081/1‑149 (MQ=17)
                                                                                     aaaaCGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                                           <  1:329632/149‑1 (MQ=32)
                                                                                      aaaCGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGACGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCcgccgc                                          >  2:87261/1‑149 (MQ=17)
                                                                                         cGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGGAGATGCTCGACCGGCTGGAAAGGGGAATCGTGGCTGGCCGGGTGAGTGAGGGAAGCCGCGgcagg                                       >  2:57728/1‑149 (MQ=11)
                                                                                                 cgcAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATc                               <  2:430374/149‑1 (MQ=32)
                                                                                                  gcAGCCTGTCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCg                              >  1:571563/1‑149 (MQ=17)
                                                                                                  gcAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCGGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCACTGGCTGGCATCg                              >  2:604102/1‑149 (MQ=17)
                                                                                                  gcAGCCTGGCGGATACCCTTCAGCAGTCAGATTGGTAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCg                              >  2:363716/1‑149 (MQ=11)
                                                                                                       cTGGCGGATACCCTTCAGCAGTCTGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg                         <  1:29130/149‑1 (MQ=17)
                                                                                                       cTGGCGGATACCCTTCAGCAGTCTGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCgg                         <  1:28781/149‑1 (MQ=17)
                                                                                                                              cGGTGGCGAAGACAGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCaaa  >  1:533371/1‑149 (MQ=25)
                                                                                                                              cGGGGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCaaa  <  2:402012/149‑1 (MQ=17)
                                                                                                                              |                                                                                                                                                    
CGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATACCCTTCAGCAGTCCGGTGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACGGTGGCGCAGATGCAAA  >  NC_000913/732699‑732973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: