Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,485,617 C→A 10.1% H853N (CAT→AAT)  hrpA → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,485,6170CA10.1% 100.7 / 5.5 40H853N (CAT→AAT) hrpAputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (20/16);  new base A (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

GCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTGCGCCGCGAA  >  NC_000913/1485471‑1485763
                                                                                                                                                   |                                                                                                                                                  
gCCACTACGTACTCACCCCCCGCCACTACCACTGCTAGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCAt                                                                                                                                                  <  1:9964/149‑1 (MQ=255)
 ccACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATc                                                                                                                                                 >  2:300219/1‑149 (MQ=255)
      aTGTAATCTCCGCTCGCCATTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAACCGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGc                                                                                                                                            <  1:440765/149‑1 (MQ=255)
           aTCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCCACTTTG‑AAAAAAGAATGTTGATCAAAGCGGGCGCAGAAAAAATAAGCAAGCTGGATAACCCGAAATTCTGGCATCAGGGCAAtct                                                                                                                                       >  2:266461/1‑149 (MQ=255)
           aTCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAAtct                                                                                                                                       >  1:697514/1‑149 (MQ=255)
           aTCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCACCAGGGCAAtct                                                                                                                                       >  2:143555/1‑149 (MQ=255)
                         cTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGa                                                                                                                         >  1:537571/1‑149 (MQ=255)
                         cTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGa                                                                                                                         >  1:574976/1‑149 (MQ=255)
                                 gCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGAATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCCCAAGCTGCGTTTGAGCTATCAg                                                                                                                 >  1:186970/1‑149 (MQ=255)
                                    gggggAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTAAACTATG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGttt                                                                                                              <  2:111992/146‑1 (MQ=255)
                                         aaaaaaGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGAATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGcc                                                                                                         >  2:44383/1‑149 (MQ=255)
                                         aaaaaaGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGAATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGcc                                                                                                         >  2:295568/1‑149 (MQ=255)
                                                tCAGCCGCGAAGCGCCTGATTTGCTCAACTTTGAAAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCCCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGgcgc                                                                                                   >  2:136120/1‑149 (MQ=255)
                                                tCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGGCCCATCAGTTTGAGCCCGGcgcg                                                                                                  >  2:136082/1‑149 (MQ=255)
                                                tCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGcgcg                                                                                                  >  1:394571/1‑149 (MQ=255)
                                                       cgAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTg                                                                                           >  1:232044/1‑149 (MQ=255)
                                                              ccTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGCTTGAGCTATCAGTTTAAGCCCGGCGCGGATGCTGACGgtgt                                                                                    >  2:539021/1‑149 (MQ=255)
                                                                             tttG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTAACCGAAATTCTGGCAACAGGGCAATCCCAAGCTGCGTTTGCGCTATCAGGTTGAGCCCGGCGCGGATGCTGACGGTTGGACCGAACATCTTcc                                                                     >  2:671224/1‑149 (MQ=255)
                                                                                    aaaaGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGcc                                                               >  2:542333/1‑149 (MQ=255)
                                                                                    aaaaGCATGTTGACCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGcc                                                               <  2:597429/149‑1 (MQ=255)
                                                                                        gCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCGATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTa                                                           <  1:300219/149‑1 (MQ=255)
                                                                                        gCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGAATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTa                                                           <  1:44383/149‑1 (MQ=255)
                                                                                        gCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGAATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTa                                                           <  1:295568/149‑1 (MQ=255)
                                                                                           tGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACtt                                                        >  2:261137/1‑149 (MQ=255)
                                                                                                    aaGCGGGCGCAGAAAACATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGtt                                               <  1:650961/149‑1 (MQ=255)
                                                                                                      gagGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACCTCCCCCGCTGCCGTTACTTAACCAGGTTGa                                             <  2:397633/149‑1 (MQ=255)
                                                                                                                          cAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGc                         <  2:232044/149‑1 (MQ=255)
                                                                                                                             gCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGa                      <  1:694098/149‑1 (MQ=255)
                                                                                                                              cTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGAt                     <  2:574976/149‑1 (MQ=255)
                                                                                                                              cTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGAt                     <  2:537571/149‑1 (MQ=255)
                                                                                                                              cTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGAt                     >  1:341536/1‑149 (MQ=255)
                                                                                                                              cTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTGTGAATGGCAGAt                     >  1:341591/1‑149 (MQ=255)
                                                                                                                                 gATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATccc                  >  2:285972/1‑149 (MQ=255)
                                                                                                                                 gATTACCCGAACTTCTGGCATCAGGGCAATCTAAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATccc                  >  2:561942/1‑149 (MQ=255)
                                                                                                                                   ttACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTGGAGGAAAGCGGGGTTGCATGGCAGATCCCCg                >  2:526471/1‑149 (MQ=255)
                                                                                                                                        cGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTg           <  2:203706/149‑1 (MQ=255)
                                                                                                                                        cGAACTTCTGGCATCAGGGCAATCCCAAGCTGCGTTTGAGCTAGCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGTTTTAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTg           <  2:7300/149‑1 (MQ=255)
                                                                                                                                                 ggCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGATTGGCAGATCCCCGGTCTGCGCCGCGaa  <  2:558380/149‑1 (MQ=255)
                                                                                                                                                 ggCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTGCGCCGCGaa  <  2:697514/149‑1 (MQ=255)
                                                                                                                                                 ggCATCAGGGCAACCTAAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTGCGCCGCGaa  <  1:143555/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTCAGCCGCGAAACGCCTGATTTGCTCAACTTTG‑AAAAAAGCATGTTGATCAAAGAGGGCGCAGAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGTTTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTGCCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTGCGCCGCGAA  >  NC_000913/1485471‑1485763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑