Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,746,100 G→A 11.6% R28R (CGC→CGT)  rpsP ← 30S ribosomal subunit protein S16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,746,1000GA11.6% 118.4 / 6.7 50R28R (CGC→CGT) rpsP30S ribosomal subunit protein S16
Reads supporting (aligned to +/- strand):  ref base G (31/13);  new base A (3/3);  total (34/16)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TTCTTTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATT  >  NC_000913/2745953‑2746246
                                                                                                                                                   |                                                                                                                                                  
ttcttTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGc                                                                                                                                                   >  2:704695/1‑149 (MQ=255)
  cttTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGt                                                                                                                                                 >  1:378140/1‑149 (MQ=255)
  cttTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGt                                                                                                                                                 >  1:127321/1‑149 (MQ=255)
  cttTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGt                                                                                                                                                 >  1:687864/1‑149 (MQ=255)
  cttTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTAAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGt                                                                                                                                                 >  1:737451/1‑149 (MQ=255)
           gcgcAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACgg                                                                                                                                        <  1:292545/149‑1 (MQ=255)
                 caACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATCACGGCTGTcg                                                                                                                                  >  2:51810/1‑148 (MQ=255)
                       gATCAGAAATAGTTGCGCCCTGGCCAAGCCACCTAGCGATGCTATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCACCGGGCTCGATGAAGCGACCGTTGCGTGCATTACTGCTGTCAGCAaca                                                                                                                            >  1:383482/1‑149 (MQ=255)
                                tAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTgg                                                                                                                   <  2:393865/149‑1 (MQ=255)
                                  gtcgcgcCCTGTCCACCCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGCTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTa                                                                                                                 <  2:449145/146‑1 (MQ=255)
                                         ccTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACCACAACCTGGTAGAACGGa                                                                                                          >  2:132273/1‑149 (MQ=255)
                                         ccTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACCACCTGGTACAACGGa                                                                                                          >  1:643539/1‑149 (MQ=255)
                                         ccTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGa                                                                                                          >  1:630130/1‑149 (MQ=255)
                                             gCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCCGCGACAACAACCTGGTAGAACGGACGCt                                                                                                      >  2:784688/1‑149 (MQ=255)
                                                  cccAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTa                                                                                                 <  2:378140/149‑1 (MQ=255)
                                                  cccAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTa                                                                                                 <  2:737451/149‑1 (MQ=255)
                                                  cccAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTa                                                                                                 <  2:687864/149‑1 (MQ=255)
                                                  cccAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTACGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTa                                                                                                 <  2:127321/149‑1 (MQ=255)
                                                       tGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTTGCGTTTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTGGAATGGACGCTTTTTGGCGcc                                                                                            >  1:300355/1‑149 (MQ=255)
                                                       tGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGCCAACAACCTGGTAGAACGGACGCTTTTTAGCGcc                                                                                            >  1:645503/1‑149 (MQ=255)
                                                       tGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAgggag                                                                                            >  2:582307/1‑145 (MQ=255)
                                                       tGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTCTTAGCGcc                                                                                            >  1:456302/1‑149 (MQ=255)
                                                                     cagatccagGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAAc                                                                              >  1:484585/1‑149 (MQ=255)
                                                                     cagatccagGCGACTGCCTTCTTCTTTTTCGCTAGCGATAGGGTTGAAGCAACCAACGCGCTCGATGCAACGACCGTTGCGTGCATTACGGATGTCAGCGGCAACACGCTGATAGAACGGACGCTTTTTAGCGCCGTGACGGGCTAAAc                                                                              >  2:229191/1‑149 (MQ=255)
                                                                     aagatccagGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGGGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGAGAACAACCTGGTAGAGCGGGCGCTTTTTCGCGCCGTGACGGGCTAAAc                                                                              >  2:523250/2‑149 (MQ=255)
                                                                        atccagGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGaa                                                                           >  1:113691/1‑149 (MQ=255)
                                                                              gCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTa                                                                     <  1:523072/149‑1 (MQ=255)
                                                                                           tcttTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCctc                                                        <  1:76088/149‑1 (MQ=255)
                                                                                             tttttCGCTAGAGATTGGTTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCtt                                                      >  2:482035/1‑149 (MQ=255)
                                                                                               tttCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTtgt                                                    <  2:594439/149‑1 (MQ=255)
                                                                                               ttgcgatAGCGATGGGGTTGAAGAAACCAAGGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGAGAACTACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTGACCATAACATCCTCTtgt                                                    <  2:694893/143‑1 (MQ=255)
                                                                                                 tCGCTAGCGATTGGGTTGAAGAAACCACCGCGGTCGATGTAGCGACCGTTGCGTGCTTTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTtgtgt                                                  <  2:740408/149‑1 (MQ=255)
                                                                                                    cTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGaa                                               >  1:598594/1‑149 (MQ=255)
                                                                                                    cTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTAAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGCGTGaa                                               >  1:176676/1‑149 (MQ=255)
                                                                                                     tAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAAt                                              <  2:608471/149‑1 (MQ=255)
                                                                                                        cGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTGTTGTGGGAATaaa                                           >  1:711942/1‑149 (MQ=255)
                                                                                                              ggTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAAcc                                     >  1:516793/1‑149 (MQ=255)
                                                                                                                    agaaACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGGccc                               >  1:223816/1‑149 (MQ=255)
                                                                                                                        aCCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATc                           >  1:162278/1‑149 (MQ=255)
                                                                                                                        aCCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATc                           >  1:89303/1‑149 (MQ=255)
                                                                                                                        aCCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGGCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATc                           >  1:188440/1‑149 (MQ=255)
                                                                                                                              gcgcTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGaa                     <  2:113691/149‑1 (MQ=255)
                                                                                                                               cgcTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACGTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAAc                    >  1:416017/1‑149 (MQ=255)
                                                                                                                               cgcTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCCTCGAGGAAc                    >  1:142765/1‑149 (MQ=255)
                                                                                                                               cgcTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAAc                    >  1:558232/1‑149 (MQ=255)
                                                                                                                                gcTCGATGAAGCGACGGTTGCGTGCCTTACGGCTGTCAGCGACAACAACCTGGTAGAACGGTCGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACg                   <  2:32752/149‑1 (MQ=255)
                                                                                                                                            gACCGTTGCGTGCATTAGGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATACTCTTGTGTGAATAAAACAACCGGCCCCCATCGCGGAACGGCGTCCGGTGTc       >  2:679280/1‑149 (MQ=255)
                                                                                                                                                gTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCatat   <  1:711534/149‑1 (MQ=255)
                                                                                                                                                 ttGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATAtt  >  1:538573/1‑149 (MQ=255)
                                                                                                                                                 ttGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACAACCTCTTGTGTGAATAAAAAAACCGGACCCCATCGAGGAACGGAGTCCAGCGTCATAtt  >  1:395942/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCTTTGATCAGCGCAGCAACGCGATCAGAAATAGTTGCGCCCTGGCCAACCCAGTGAGCGATGCGATCCAGATCCAGGCGAGTGCCTTCTTCTTTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATT  >  NC_000913/2745953‑2746246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑