Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,858 A→G 13.0% noncoding (59/78 nt) micA → sRNA antisense regulator of ompA, lamB, ompX, and phoP; Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,8580AG13.0% 82.5 / 7.4 44noncoding (59/78 nt)micAsRNA antisense regulator of ompA, lamB, ompX, and phoP; Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base G (4/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-02

GCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCT  >  NC_000913/2814714‑2815007
                                                                                                                                                |                                                                                                                                                     
gCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTATGAGGATATGAAAGACGCGCATTTGTTATCATCATCACTGAATTCAGAGCTCAAATTTTGGCAACTAAcacg                                                                                                                                                   >  2:463815/1‑146 (MQ=255)
    tCTAACAACGGCATGTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGc                                                                                                                                               <  1:648635/149‑1 (MQ=255)
      tAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGCGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGCAATTTTGGCCACTCACGAATGGCCt                                                                                                                                             >  2:493669/1‑149 (MQ=255)
        acaacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCATGCGAGTCTGGGTATATGAAAAACGCGCATTTCTTATCATCATCCCTGAACTCAGCAATGAAATTTTGGCCCTTCACGCGTGCCCtct                                                                                                                                           >  1:580431/1‑147 (MQ=255)
         caacGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCtttt                                                                                                                                          <  2:676331/149‑1 (MQ=255)
           ccGGCATTTAGCCCCCGCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGTGAGTGGccaaaatt                                                                                                                                        <  1:320608/148‑7 (MQ=255)
           acGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGTGAGTGGccaaaatt                                                                                                                                        <  1:122852/149‑7 (MQ=255)
            cGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttct                                                                                                                                       >  1:346491/1‑149 (MQ=255)
            cGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCAGCCCTGAATCCAGAGATGAAATTTTGGCCACGCACGAGTGGCCTttttct                                                                                                                                       >  1:353002/1‑149 (MQ=255)
                     gCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCCGACTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCa                                                                                                                              <  1:347963/149‑1 (MQ=255)
                       cACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAgg                                                                                                                            >  1:450739/1‑149 (MQ=255)
                           tCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAATATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCgtg                                                                                                                        >  2:421253/1‑149 (MQ=255)
                                   tctttttAAAAATTTTCTGACCTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCa                                                                                                                <  2:779018/147‑1 (MQ=255)
                                    ttttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAg                                                                                                               >  1:589122/1‑149 (MQ=255)
                                     tttttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGc                                                                                                              <  2:458857/149‑1 (MQ=255)
                                      ttttAAACTTTTTCTGTACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGcc                                                                                                             <  1:449305/149‑1 (MQ=255)
                                       tttAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCca                                                                                                            <  1:627700/149‑1 (MQ=255)
                                                        ctctTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCGCGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCgg                                                                                           >  2:406301/1‑149 (MQ=255)
                                                         tctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACGCCGCACACGGTTCgtg                                                                                          >  1:698966/1‑147 (MQ=255)
                                                           tttcttCCCAGGCGAGTCTGAGTATATGATAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTAt                                                                                        <  2:496658/149‑1 (MQ=255)
                                                              cttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTTCCGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCTAACGGTTCGGTATCAg                                                                                     <  1:495496/149‑1 (MQ=255)
                                                                            cTGAATATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCAACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTcc                                                                       <  1:421253/149‑1 (MQ=255)
                                                                                      gAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTG‑TTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCg                                                            >  2:337978/1‑149 (MQ=255)
                                                                                        aaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACTGCGt                                                           >  1:246046/1‑149 (MQ=255)
                                                                                        aaGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGt                                                           <  2:107340/149‑1 (MQ=255)
                                                                                         aGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCTAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGtt                                                          >  1:741152/1‑149 (MQ=255)
                                                                                          gACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTc                                                         <  1:463815/149‑1 (MQ=255)
                                                                                           aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGCCGGCGTCCa                                                        >  2:551089/1‑149 (MQ=255)
                                                                                                     gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCCGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTAAGACGCCTcgc                                              >  1:283288/1‑149 (MQ=255)
                                                                                                     gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTCCCTGCTTCCGGACCTCACACACCTcgc                                              >  1:324791/1‑149 (MQ=255)
                                                                                                           atcatcCCTGAATTCAGAGATGAAGTTTTGGCCACTCACGGGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGGCACACCGCGAACGGTTCGGTATCAGCGGTTTCCGTTTGCTGCTGACGGCGTTCAGACGACTCGCGCTCgg                                        >  2:349915/1‑149 (MQ=255)
                                                                                                           atcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                        <  2:102866/149‑1 (MQ=255)
                                                                                                           atcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                        <  1:705101/149‑1 (MQ=255)
                                                                                                                 ccTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGCCGGCGTTCAGGCGCCTCGCGCCCGGCTagca                                  >  1:252297/1‑146 (MQ=255)
                                                                                                                 ccTGAATTCAGAGATGAAATTTTGGCCACTCACGACTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCCCGGCTACaa                                  >  2:397572/1‑149 (MQ=255)
                                                                                                                       ttCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACCCCGCACACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTCACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAAACct                            >  1:119057/1‑149 (MQ=255)
                                                                                                                           gagaTGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTc                        <  1:567720/149‑1 (MQ=255)
                                                                                                                            agaTGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTcg                       <  1:322309/149‑1 (MQ=255)
                                                                                                                                   aaaaaggCCACTCGTGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGaa                >  1:675569/6‑149 (MQ=255)
                                                                                                                                      aaggCCACTCGTGAGTGGCCTTTTTCTTTTCTGTCAGGCGGGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGCCGCCTCGCGCTCGGCTACCAAATCCTCTTCGCGCAGAAttt             >  1:323177/3‑149 (MQ=255)
                                                                                                                                      aaggCCACTCGTGAGTGGCCTTTGTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGTAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTGGGCTACAAAATCCCCTTCGCGCCGAAttt             >  2:576754/3‑149 (MQ=255)
                                                                                                                                             cTCACGTGTGGCCTTTTTCTTTTCTGTCAGTCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCgg      <  2:580431/149‑1 (MQ=255)
                                                                                                                                              tCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCTCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGc     >  1:605000/1‑149 (MQ=255)
                                                                                                                                              tCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCCCGGCTACAAAATCCTCTTCGCGCAGAATTCCCAGCgac     >  1:637453/1‑147 (MQ=255)
                                                                                                                                                aCGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACTGTTCGGTATCAGCGGCTTCCCTTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCCCAGCATTTCCACCGGctc   >  2:692101/1‑149 (MQ=255)
                                                                                                                                                aCGAGTGGCCTTTTTCTTTTCTGTCAGGCGTG‑TTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGctct  <  1:337978/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCT  >  NC_000913/2814714‑2815007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑