Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,486,769 T→C 7.7% intergenic (+19/‑20) crp → / → yhfK cAMP‑activated global transcription factor, mediator of catabolite repression/putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,7690TC 7.7% 131.1 / 3.9 53intergenic (+19/‑20)crp/yhfKcAMP‑activated global transcription factor, mediator of catabolite repression/putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (27/21);  new base C (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-02

CCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTC  >  NC_000913/3486624‑3486917
                                                                                                                                                 |                                                                                                                                                    
ccGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTAc                                                                                                                                                   >  1:652513/1‑149 (MQ=255)
     aGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGt                                                                                                                                              <  2:549552/149‑1 (MQ=255)
     aGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGt                                                                                                                                              <  1:278265/149‑1 (MQ=255)
     aGGAAATTGGTCAGATTGTCGGCTGTGCTCGTGAAACCGTGGGGCGCATGCCGAAGATGCTGGAAGATCAGAACCTGATCCCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGt                                                                                                                                              <  1:549651/149‑1 (MQ=255)
       gAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAg                                                                                                                                            >  1:716499/1‑149 (MQ=255)
              gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGCTACCAGGTAACACGCCa                                                                                                                                     <  2:668840/149‑1 (MQ=255)
              gTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCCGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGCTACCAGGTAACACGCCa                                                                                                                                     <  2:55352/149‑1 (MQ=255)
                    ttGTCGGCTGTCCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                                                                               <  1:149384/149‑1 (MQ=255)
                          gcatttttcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGACCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCGTTTTGTTTcccc                                                                                                                         >  2:97076/8‑149 (MQ=255)
                              ttCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATGCCGTCTGTGTGGCGCGTTTCCTGGTAGCGCGCCCTTTTGTTTCCCCCGAt                                                                                                                     >  1:300176/1‑149 (MQ=255)
                                ctcGTGAAACCGTGGGACGCATTCTGAAAATGCTCGAAGATCAGAACCTGATCGCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtgt                                                                                                                   >  1:430998/1‑149 (MQ=255)
                                 tcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtgtg                                                                                                                  >  1:269071/1‑149 (MQ=255)
                                 tcGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGAtgtg                                                                                                                  >  1:561478/1‑149 (MQ=255)
                                  cGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAGCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTgg                                                                                                                 <  2:536907/149‑1 (MQ=255)
                                    tGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                                                                               <  1:553841/149‑1 (MQ=255)
                                    tGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGACCAGAACCAGATCTCCGCACACGGTAACAAACTAGGCTGGTACGGCGATGGTTTGTCCCTTCGGAGTGGCGCGTTACCTTGTAGCGCGCCATTTTGTTTCCCCCGATGTGgcg                                                                                                               <  1:573667/149‑1 (MQ=255)
                                           gTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAACCCGTCGTCGTTTACGGCACTCGTTAATCCCGTCGGAATGGGGCGTGACCGGGTAGGGCGCCCTTTTGTTTCCCCCGATGTGGCGCAGACTg                                                                                                        >  1:180046/1‑149 (MQ=255)
                                             gggACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAt                                                                                                      <  2:130450/149‑1 (MQ=255)
                                               gACGCATTCTGCAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAttt                                                                                                    <  2:114112/149‑1 (MQ=255)
                                               gACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAttt                                                                                                    <  1:170635/149‑1 (MQ=255)
                                                 cGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTAt                                                                                                  <  2:499696/149‑1 (MQ=255)
                                                  gCATTCTGGAGATGCTGGAAGATCAGAACCTTATCTGCGCACACGGTAAACCCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATc                                                                                                 <  2:21289/149‑1 (MQ=255)
                                                            gATGCCGGAAGATCAGAACCTGATCTACGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGatat                                                                                       <  1:322529/149‑1 (MQ=255)
                                                             aTGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATc                                                                                      >  2:17505/1‑149 (MQ=255)
                                                             aTGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATc                                                                                      <  1:716545/149‑1 (MQ=255)
                                                               gCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCaa                                                                                    >  1:169268/1‑149 (MQ=255)
                                                                  ggAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTa                                                                                 >  2:574526/1‑149 (MQ=255)
                                                                    aaGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATg                                                                               <  2:32972/149‑1 (MQ=255)
                                                                     ataTCAGACCCGGTTCCCCGCACAGGGTAAAACCCTCGTGGTTTACGGCCCTCGTTAATCCCGCCGGAGTGGCGCGTTACCGGGTAGCTCGCCATTTTTTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGc                                                                              <  1:199175/147‑1 (MQ=255)
                                                                     aGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGc                                                                              >  2:554266/1‑149 (MQ=255)
                                                                       aTCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCAc                                                                            >  2:12686/1‑149 (MQ=255)
                                                                          aGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCCACTATGCACTTc                                                                         >  2:132401/1‑149 (MQ=255)
                                                                           gAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGGCTGATTTATCACCCCGATATCAACTATGCGCTTCg                                                                        >  2:580189/1‑149 (MQ=255)
                                                                           gAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGCCTGATTTATCACCCCGATATCAACTATGCACTTCg                                                                        >  2:228019/1‑149 (MQ=255)
                                                                                     tcCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTCCCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGAGATCAACTATGCACTTCGACAAACGCTg                                                              >  1:626690/1‑149 (MQ=255)
                                                                                          acacGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGGCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCt                                                         >  2:34107/1‑149 (MQ=255)
                                                                                                      cATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTATCTGGTAGCGGGATATTTTGTTTCTACCGATGTGGCGCAGAGTGATTTATCACTCCGATATCAACTATGCACTCCGACAAACCCTGGTGCTATGTTTGCCCGt                                             >  2:573852/1‑149 (MQ=255)
                                                                                                         cgtcgtTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGc                                          >  2:87147/1‑149 (MQ=255)
                                                                                                         cgtcgtTTACGGCACTCGTTAATCCCGTCGGAGTGACGCGGTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGCCTGATTTATCACCCCGCTATCAACTATGCCCTTCGCCAAACGCTGGTGCTATGTTTGCCCGTGGc                                          >  2:367696/1‑149 (MQ=255)
                                                                                                           tcgtTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCg                                        >  2:769753/1‑149 (MQ=255)
                                                                                                                 aCGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGt                                  >  2:701596/1‑149 (MQ=255)
                                                                                                                   ggCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGCTTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTa                                >  2:274399/1‑149 (MQ=255)
                                                                                                                    gCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTaa                               <  2:691366/149‑1 (MQ=255)
                                                                                                                      gcTCGTTAATCCCGGCGGAGTGGCGCGTTACCTGGTGGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATg                             <  1:213790/148‑1 (MQ=255)
                                                                                                                           ttAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCCCTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTgg                        >  2:638922/1‑149 (MQ=255)
                                                                                                                               tCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACGTCGACAAACGCTGGTGCCATGTTTGCCCGTGGCCGGTGGGTTAATGCTTGGCGaa                    >  2:379478/1‑149 (MQ=255)
                                                                                                                                   gTCGGAGTGGCGCGTTACCTGGTAGCGCGCCCTTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTGTGCCCGTGGCCGCTGGGTTAATGCTTGGCGAATTAc                >  2:295637/1‑149 (MQ=255)
                                                                                                                                   gTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTAc                >  2:92134/1‑149 (MQ=255)
                                                                                                                                        aatGGCGCGCTACCAGGTAACGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTc           >  1:410683/3‑149 (MQ=255)
                                                                                                                                        aatGGCGCGCTACCAGGTAACGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCCATGTTTGCCCGGTGCCGGTGGGTTAATGCTTGCCGAATTACGATTc           >  1:262283/3‑149 (MQ=255)
                                                                                                                                         gTGGCGCGTTACCTGGTAGCGCGCCATTTGGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTCTGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCg          <  1:85391/149‑1 (MQ=255)
                                                                                                                                           ggCGCGTTACCTGGGAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGt        <  1:203276/149‑1 (MQ=255)
                                                                                                                                            gcgcgcTACCAGGTAACGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAACGCGTGGCGAATTACGATTCGGTc       >  2:784786/1‑149 (MQ=255)
                                                                                                                                              gcgTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTg     <  1:12686/149‑1 (MQ=255)
                                                                                                                                                 ttACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGctc  <  1:554266/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTGGCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTC  >  NC_000913/3486624‑3486917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑