Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,621 A→G 11.5% intergenic (+87/‑109) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,6210AG11.5% 98.4 / 7.3 44intergenic (+87/‑109)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (16/22);  new base G (2/3);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-02

ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGG  >  NC_000913/164473‑164767
                                                                                                                                                      |                                                                                                                                                  
aTGTCTGGCCGGACGACCCCGCACACACCGCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCTCACCCGCACCCCCTCCCTCa                                                                                                                                                    >  2:285225/1‑149 (MQ=37)
   tCTGGCCGGACGGCCGGGCAAATTCTGCACCGGCGCGACGGACGAAAAGGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                                                                                 <  2:194003/149‑1 (MQ=38)
     tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCCAgggg                                                                                                                                               >  2:20516/1‑148 (MQ=255)
          tgacgacCCGGCAAATACTGTACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                                                          <  1:713412/148‑1 (MQ=255)
           gacgacCCGGCAAATACTGCACCGACGCGACGGCCGACAGCGACTGCGTTGGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggt                                                                                                                                         <  1:256476/149‑2 (MQ=25)
           gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggt                                                                                                                                         <  1:730827/149‑2 (MQ=255)
           gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggt                                                                                                                                         <  1:71960/149‑2 (MQ=255)
             cgacCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                                                                       <  1:453473/149‑1 (MQ=255)
              gacCCCGCAAATACTGCTCCGACGCGACAGACGAAAAAGTCTTCGTAAGGTCGGTTT‑‑TCTCCCTCTCCCGGGCGGCGTGGGACGTGGCGCGGGCATCAGCGCGCACGTTCACCCGCCCCCTAACCCCACCCCGCAAGGGCAAGGGGccc                                                                                                                                      >  2:38030/1‑149 (MQ=255)
                 ccGGCAAATACTGCCCCGTCGCGACGGCCGAACAAGTATTCGTAAGGTTGGTTT‑‑CCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                                                                   <  1:53945/149‑1 (MQ=38)
                 ccGGCAAAGGCTGCACCGTCTCGTCGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCATCTCTCACTTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                                                                   <  1:463195/149‑1 (MQ=37)
                  cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCCCCCTCAAGGGAGAGGGGACCGATc                                                                                                                                  >  1:236337/1‑149 (MQ=255)
                              cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt                                                                                                                        <  1:45088/149‑1 (MQ=255)
                                ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg                                                                                                                    >  1:51656/1‑149 (MQ=255)
                                             cgAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGCCCGATCGAGCACAAAGTTTGAGAGATAtcttct                                                                                                       >  2:325597/1‑149 (MQ=255)
                                                aaaaGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCCAGCACAAATTTTGAGAGATAtcttcttct                                                                                                    >  2:503800/1‑149 (MQ=255)
                                                                ttggttT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga                                                                                    <  2:728880/149‑1 (MQ=255)
                                                                  tgttT‑‑TCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                                                                  <  1:361041/148‑1 (MQ=255)
                                                                  ggttT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                                                                  <  1:2924/149‑1 (MQ=255)
                                                                             cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                                                         <  2:87767/149‑1 (MQ=255)
                                                                             cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                                                                         >  1:41071/1‑149 (MQ=255)
                                                                             cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGATGACGCTCACCCTAACCCTCTCCCTCACGGGAGGGGGGACCGATCGAGCGCAAATTTTGAGAGTTATCTTCTTCTGTCTTCTAAGAGCAGAACCGAACATCg                                                                         >  1:51292/1‑149 (MQ=37)
                                                                                   cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGCACAGAAAAGCGGGCgtt                                                                   <  2:600301/149‑1 (MQ=255)
                                                                                     tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                                 >  2:421714/1‑149 (MQ=255)
                                                                                     tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                                 >  1:464908/1‑149 (MQ=255)
                                                                                               ggCCGGGGTGAGGGCATCAGCGCGCACGTCCCCCCCACCCCTAAGCCGCTGCCTCAAGGGAGAGGGGACCGATCGAGCACAAATCTTGAGACATATCCTCTTCCGCCTTGTAACAGAAGCACAGAACATCCGGCTTTTGCGACtccatc                                                       <  2:748512/149‑6 (MQ=255)
                                                                                               ggCCGGCGGGAGCGCATCAGCGCGCACGTTCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGCAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata                                                       <  2:49048/149‑1 (MQ=255)
                                                                                                 ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt                                                     >  2:590472/1‑149 (MQ=255)
                                                                                                    gggTGCGGGCCTCAGCGCGCACGTGCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGGCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                                  <  1:484482/149‑1 (MQ=255)
                                                                                                    gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCCCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                                  <  1:20516/149‑1 (MQ=255)
                                                                                                      gTGAGGGCAGCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga                                                <  1:98399/149‑1 (MQ=255)
                                                                                                      gTGACGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga                                                <  2:570322/149‑1 (MQ=255)
                                                                                                            gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa                                          <  2:602038/149‑1 (MQ=255)
                                                                                                            gCATCAGCGCGCAAGTTCACCCTCACCCTAACCCTCTCCCTAAAGGGAGGGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa                                          >  1:560870/1‑149 (MQ=255)
                                                                                                                  gcgcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATgg                                    >  1:703716/1‑149 (MQ=255)
                                                                                                                    gcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAGAAGCATGGcc                                  >  1:476570/1‑149 (MQ=255)
                                                                                                                        aCGTTCACCCTCACCCTAACCCTCTCCCTGAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAGAGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa                              >  1:550536/1‑149 (MQ=255)
                                                                                                                                        gtccccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAt              >  2:749540/4‑149 (MQ=255)
                                                                                                                                           cccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTgg           >  2:741740/1‑149 (MQ=255)
                                                                                                                                                tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGCCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCa      <  1:279947/149‑1 (MQ=255)
                                                                                                                                                 cccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaa     >  2:101587/1‑149 (MQ=255)
                                                                                                                                                   cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATACCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGAGAGCCAATTGGACGCAAAg   <  1:310439/149‑1 (MQ=255)
                                                                                                                                                    tCAGGGGAGAGGGGGCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCGTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg  <  2:309417/149‑1 (MQ=255)
                                                                                                                                                    tCAAGGGAGAGGGGACCGATCGAGCCAAACTGTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg  >  1:777073/1‑149 (MQ=255)
                                                                                                                                                    tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg  <  2:747523/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTT‑‑TCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGG  >  NC_000913/164473‑164767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: