| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,790,302 | A→T | 8.4% | intergenic (‑222/+17) | waaH ← / ← tdh | LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,790,302 | 0 | A | T | 8.4% | 174.8 / 5.6 | 58 | intergenic (‑222/+17) | waaH/tdh | LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (24/29); new base T (2/3); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCC > NC_000913/3790158‑3790448 | tattTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAAAAGCTTCTCCTTTCATAATAGGGCTGGAATACAAg > 1:726113/1‑149 (MQ=255)tattTTTACAGGTGAGAGAGTCACTTCAGAGTAGAGAAATAATGAGAGCAGCTATATAAGAGACCAGCACAATTACGAACTCTTTATTGCACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAg > 1:508235/1‑149 (MQ=255) attTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGc < 2:231903/149‑1 (MQ=255) tttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGGTAAAAGGGCTGGTATTCCAGCCCt < 1:581881/149‑1 (MQ=255) tttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGTATTCCAGCCCt < 1:681403/149‑1 (MQ=255) ttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCtt < 1:330913/149‑1 (MQ=255) ttACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCtt < 2:17034/149‑1 (MQ=255) gTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCCGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATACAAGGGCTGGAATACCAGCCCTTGTTCgt < 1:431518/149‑1 (MQ=255) gagagaGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt < 1:111151/149‑1 (MQ=255) gagagaGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCgtgt < 1:446797/149‑1 (MQ=255) gTCACTTCAGTGTAGAGACATAATGAGAGCAGCTATATAAGAGATCCGCACAATTACGAACTCTTTATTACACTGCCAGGCTTTTACATATTTAACCGATTATCCTCAGATACAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATcc < 2:422141/149‑1 (MQ=255) ttCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCt < 2:763653/149‑1 (MQ=255) gTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACTAGCCCTTGTTCGTGTTAATCCCAGCTCAGAAt > 1:584835/1‑149 (MQ=255) tAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATa < 1:247439/149‑1 (MQ=255) agagaTAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACt > 2:597299/1‑149 (MQ=255) agagaTAATGAGAGCAGCCATATAAGACATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACt > 2:636021/1‑149 (MQ=255) tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTTACAGGATTTTACATATTTAACAGATTATCCTCAGATAAACGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGa < 2:255075/149‑1 (MQ=255) tGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGa > 1:306159/1‑149 (MQ=255) agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGccc < 2:741360/149‑1 (MQ=255) agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGccc < 2:741606/149‑1 (MQ=255) agcTAAATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGccc < 2:73625/149‑1 (MQ=255) cTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGa > 1:525196/1‑149 (MQ=255) tatataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGaa > 2:50785/1‑149 (MQ=255) tataTGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg < 2:569247/149‑1 (MQ=255) tataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg < 2:179735/149‑1 (MQ=255) tataAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACg < 2:728965/149‑1 (MQ=255) aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCAt > 2:427543/1‑149 (MQ=255) aaGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGAGTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAAAATAACTTTCCCGGACTGGCCCGAACGCaa > 2:427552/1‑148 (MQ=255) aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATGCGCAGATAAAGGGTGTGTAGTTCCAGCGCTTGTTCGTGTTAATCCCAGCGCAGAATAACTTCCCCGGACTGGCCCGAACGCATAACGTCaa > 2:432263/1‑149 (MQ=255) aGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGCCTGGCCCGAAAGCAAAGCGTcca > 2:160580/1‑147 (MQ=255) aCGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCt < 2:495297/149‑1 (MQ=255) ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGaaa < 2:753498/149‑1 (MQ=255) ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGaaa > 1:251796/1‑149 (MQ=255) ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGaaa < 1:226094/149‑1 (MQ=255) ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTCCT‑GGaaa > 2:484101/1‑149 (MQ=255) ctctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGCTAATCCCAGCTCAGAATAACTTTCCCGGACCGGCCCGAACGCATAGCGCCAAAGCCCTTCT‑GGaca > 2:646943/1‑147 (MQ=255) ctctTTATTACACCGACAGGATTGTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGaaa < 2:80553/149‑1 (MQ=255) tttATTACACTGACAGGATTTTACATATTTAACCGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAatca < 1:276807/149‑1 (MQ=255) tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAatca < 2:104387/149‑1 (MQ=255) tttATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAatca < 1:482749/149‑1 (MQ=255) cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATag > 2:636924/1‑149 (MQ=255) cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGAGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑Gaagg > 2:325129/1‑146 (MQ=255) acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATaga < 2:142496/149‑1 (MQ=255) cTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATagag > 1:424181/1‑149 (MQ=255) aCAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACGTTCCCGGCCTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGaaa < 1:697862/149‑1 (MQ=255) ggATATTACATATTTACCAGATTTTCCTCAGAAAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAACCCCAGCTCAGAATAACTTGCCCGGACTGGCACAAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGa < 1:68540/149‑1 (MQ=255) ttACCAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCg < 2:678914/149‑1 (MQ=255) ccTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGa > 1:396695/1‑149 (MQ=255) tCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAgg > 1:421660/1‑149 (MQ=255) aTAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAg < 1:160828/149‑1 (MQ=255) aaGGGCTGGTATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGCGAATGATCGGCGAAAGATCCAGGccggcgtg > 1:773529/1‑143 (MQ=255) cTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCa > 1:243700/1‑149 (MQ=255) gAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGTAACGCTGGGTAATGATCGGGGAAAGATGCGGGCGAGACTGCCTCAgcg > 1:56919/1‑149 (MQ=255) gAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAgcg > 1:63511/1‑149 (MQ=255) aaTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAACCCCCCTGTCGGCATTAACCGGGTAGAAAAACCATCGGT‑‑TGACCGGCGACAGCTCGAGGCCAGACTGAATCAgcgc < 2:751768/149‑1 (MQ=255) aTACCAGCCCTTGTTCGTGTTCATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGAATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGcgcc < 1:353404/149‑1 (MQ=255) aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGcgcc > 2:47173/1‑149 (MQ=255) aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGcgcc > 1:709137/1‑149 (MQ=255) | TATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCT‑GGAAATCATC‑GATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCC > NC_000913/3790158‑3790448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |