Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,116,703 T→G 100% K173N (AAA→AAC glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,7030TG100.0% 232.1 / ‑6.7 69K173N (AAA→AACglpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (40/28);  minor base C (0/1);  total (40/29)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4116561‑4116847
                                                                                                                                              |                                                                                                                                                
cacgacgCACTTCTGGCAGCTTCCCGCGCGGAATATCCAGCACTTCCAGCCTTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAg                                                                                                                                            <  2:739393/148‑1 (MQ=255)
aaCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAg                                                                                                                                            <  1:27894/149‑1 (MQ=255)
 acgacgCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGa                                                                                                                                           >  1:110087/1‑149 (MQ=255)
        aCTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTAATGCATGTTCCAGATAAGcaa                                                                                                                                    >  1:7059/1‑147 (MQ=255)
          ttCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTTGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACg                                                                                                                                  <  2:671606/149‑1 (MQ=255)
           tCTGGCAGCATCCCGCGCGGAATATCCAGCACTTCCAGCATTTTGGCGTCCCAGTCCAGGGTATGGATGTTGAACAACACGGCACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGt                                                                                                                                 <  1:682647/149‑1 (MQ=255)
                 agcaTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGTTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAAc                                                                                                                           <  1:559840/149‑1 (MQ=255)
                   taTCTCGCGCGGAAGATCCAGCACTTCCAGCATTTTGTCGTGCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCg                                                                                                                         <  1:340797/148‑1 (MQ=255)
                     tctcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCTGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTa                                                                                                                       >  1:504867/1‑149 (MQ=255)
                     tctcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCCGGGTATGGATGTTCAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTa                                                                                                                       <  2:552055/149‑1 (MQ=255)
                     tctcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTa                                                                                                                       <  1:221576/149‑1 (MQ=255)
                     tctcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTa                                                                                                                       <  1:328008/149‑1 (MQ=255)
                      ctcGGGCGGAATATCCAGCACTTCCCGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACCTGGGCACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTAc                                                                                                                      <  1:154879/149‑1 (MQ=255)
                       tcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTAcc                                                                                                                     >  2:327561/1‑149 (MQ=255)
                       tcGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGCCATGTTCCAGATAAGCCACGTATCAACCGTAca                                                                                                                     >  1:639139/1‑148 (MQ=255)
                          cgcgGAATATCCCGCACTTCCGGGACTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCACGCGTACCaaa                                                                                                                  <  2:718641/149‑1 (MQ=255)
                              gAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGCACAACATGGTACGCGAGGCGTTGGTGTAATCGGGCACAGGGACAGGGCCCTGAGTCATGTTCCAGATAGCCCAGGTATCACCCATACCaacaagc                                                                                                              >  2:297491/1‑144 (MQ=255)
                              gAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAGCCGTACCAAACAGc                                                                                                              >  2:206358/1‑149 (MQ=255)
                              gAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGc                                                                                                              >  1:663548/1‑149 (MQ=255)
                              gAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGc                                                                                                              >  1:103725/1‑149 (MQ=255)
                               aaTATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCCGATAAGCCACGTGTCACCCGTACCAAACAGCa                                                                                                             >  1:662747/1‑149 (MQ=255)
                                  atCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTGAAATGCACACGGCCCTGAGACATGTTCCAGATACGCCACGGATCAACCGTACCAAACAGccaat                                                                                                          >  2:710544/1‑145 (MQ=255)
                                        cACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcaccac                                                                                                    <  1:57358/149‑1 (MQ=255)
                                          cTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCacga                                                                                                  >  1:94258/1‑149 (MQ=255)
                                               aGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCAAATGAACACAACCCTGAGTCATGTTCCAGATAAGCCACGTATGAACCGTACCAAACAGCAATTGACGCCGACTTGc                                                                                             >  1:101204/1‑149 (MQ=255)
                                                     ttGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCTTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGGATCAAGCGTACCAAACAGCAATTCACCACGACGTGCACGCTc                                                                                       <  2:519458/149‑1 (MQ=255)
                                                           tcCCAGTCCAGGGTATGGATGTTGCACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgagg                                                                                 >  1:577714/1‑148 (MQ=255)
                                                            cccAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTGTCAACCGTACCAAACAGCAATTCACCACGACCTGCAGGCTCGCGagag                                                                                >  1:484976/1‑149 (MQ=255)
                                                                gTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGAATCAACCGTACCAAACAGCAATTCACCCCGACGTGCACGCTCACGAGAGCCtt                                                                            >  1:147507/1‑149 (MQ=255)
                                                                  ccAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACAACGACGTGCACGCTCGCGCGAGCCTTcc                                                                          >  2:69712/1‑149 (MQ=255)
                                                                    aGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGAACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcac                                                                        >  2:509297/1‑149 (MQ=255)
                                                                     gggTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGGCATGTTCCAGATAAGCCCCGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGGGCCTTCcaca                                                                       >  1:206142/1‑149 (MQ=255)
                                                                          tGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTc                                                                  >  1:188081/1‑149 (MQ=255)
                                                                           ggATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTcc                                                                 >  1:422481/1‑148 (MQ=255)
                                                                             aTGTGGGACAACATGGTACGAGAGGCGTTGTTGTAATCGGTCCCATGGACACGGTCCTGAGTCATCTTCCAGATAAGCCACGCATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                               <  2:230536/149‑1 (MQ=255)
                                                                             aTGGTGAACAACAGGGTACGGGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                               <  2:39990/149‑1 (MQ=255)
                                                                               gTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGGCGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                             >  1:145495/1‑149 (MQ=255)
                                                                               gTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                             <  2:548930/149‑1 (MQ=255)
                                                                               gTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                             <  2:587941/149‑1 (MQ=255)
                                                                               gTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGa                                                             <  1:635828/149‑1 (MQ=255)
                                                                                  gAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATAAACCGTACCAAACAGCAATTCACCACGAGGTGCCCGCTCGCGGGAGCCCTCCACATGGTCGGGGATcc                                                          >  2:155058/1‑149 (MQ=255)
                                                                                    acaataTGGTACGAGAGGCGTTGGTTTAACCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCACACTAGAGCCTTCCACATGGTCGAGGATCcac                                                        <  2:557281/149‑1 (MQ=255)
                                                                                    acaacaCGGTACGAGAGGCGTTGGTGTAGTCGGTCACCTGGACACGGCCCTGCGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACGTGGTCGAGTATGcac                                                        <  2:329528/149‑1 (MQ=255)
                                                                                        caTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttca                                                    <  1:572095/149‑1 (MQ=255)
                                                                                         aTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAAAGCCTGCCACCTGTGCGAGGAGCcacgtcac                                                   >  1:748743/1‑149 (MQ=255)
                                                                                           ggTACGAGAGGCGTTGGTGTAACCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcccttcactt                                                 >  2:673898/1‑149 (MQ=255)
                                                                                             tACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCATCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTg                                               <  2:504867/149‑1 (MQ=255)
                                                                                                 agagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATCAGCTACGTATCAACCGTAGAAAACAGCAATTCACCACGCCGTGGACGCTCGCGAGAGCCTTCCACATGGCCGAGGATCCACTTCACGTTGGTGc                                           >  2:381830/1‑149 (MQ=255)
                                                                                                 agagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTCTCAACCGTACCAAACACCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCCCATGGTCGAGGATCCCCTTCACTCTCGTGc                                           >  2:501570/1‑149 (MQ=255)
                                                                                                 agagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTAAAAACCCTATCAACCAACAATTCACCACGACGGGCACGCTCGGGGGAGCCCTCCACCTGGCCGAGGCACCACTTCACTTTGGtcc                                           >  2:359136/1‑147 (MQ=255)
                                                                                                   gggCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCa                                         <  2:502223/148‑1 (MQ=255)
                                                                                                     gCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCCCTTCACTTTGGTGCCGGa                                       >  1:253718/1‑149 (MQ=255)
                                                                                                     gCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATAGACCAGGACCCACCTCACCTTGGTGCCAGa                                       >  2:708378/1‑149 (MQ=255)
                                                                                                     gCGTTGGTGTAATCGGTCACACGGACACGGCCCTGAGTCATGTTCCAGATAAGCCTCGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGTGCCTTGCACATGGTCGAGGATCCACTTTACTTTGGTGCCAGa                                       >  1:552997/1‑149 (MQ=255)
                                                                                                      cGTGGGTGTACCAGGTCACAGGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaa                                      <  1:130087/149‑1 (MQ=255)
                                                                                                        ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaaa                                    <  1:327561/149‑1 (MQ=255)
                                                                                                           gtgtAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                                 <  2:188081/149‑1 (MQ=255)
                                                                                                              tAATCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCTAGTATCCACTTCACTTTGGTGCCAGCAAAGTACgg                              >  2:436170/1‑149 (MQ=255)
                                                                                                                aTCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGCAAAGTACgggc                            >  1:192427/1‑148 (MQ=255)
                                                                                                                 tCGGTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                           >  2:643582/1‑149 (MQ=255)
                                                                                                                    gTCACATGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGACCAGTACGGGTCGAt                        >  1:71276/1‑149 (MQ=255)
                                                                                                                    gTCACATGGACACGGCCCCGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCGAt                        >  2:1695/1‑149 (MQ=255)
                                                                                                                       acaTGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAc                     <  1:84647/149‑1 (MQ=255)
                                                                                                                         aTGGACACGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAGAAGCACGGGTCAATCACCa                   >  1:579890/1‑149 (MQ=255)
                                                                                                                               acGGCCCTGAGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGGGCCAGAAAAGTACGGGTCAATCACCaaccccg             >  2:161204/1‑143 (MQ=255)
                                                                                                                                 ggCCCTGAGTCATGTTCCAGATAAGCCACGTATCACCCGTACCAAACAGCAATTCACCACGACGTGCACGCACGCGCGCGCCTTCCACATGTACGAGCGTCCACTTCACGATGGTGCAAGAAACGTACGGGTCAATCACAACACCGGTa           >  2:669031/1‑149 (MQ=255)
                                                                                                                                        aGTCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctgc    <  2:663548/149‑1 (MQ=255)
                                                                                                                                        aGTAATGTTCCAGTTAAGCCACTTAGCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctgc    <  1:297491/149‑1 (MQ=255)
                                                                                                                                          tCATGTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGGCAATCACCCGACCGGTATTGCTGCgg  >  2:412166/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
AACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4116561‑4116847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: