| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,366,856 | C→A | 11.5% | intergenic (‑82/+36) | dcuA ← / ← aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,366,856 | 0 | C | A | 11.5% | 143.2 / 11.5 | 59 | intergenic (‑82/+36) | dcuA/aspA | C4‑dicarboxylate antiporter/aspartate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base C (18/33); new base A (2/5); total (20/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01 | |||||||||||
TTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAG > NC_000913/4366711‑4366990 | ttCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCATTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTgc > 2:734155/1‑148 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATCTTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc < 1:406398/149‑1 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc < 1:377733/149‑1 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc > 2:663372/1‑149 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc < 1:181566/149‑1 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc < 2:12854/149‑1 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc < 1:434616/149‑1 (MQ=255) cccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATATTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGc > 1:755846/1‑149 (MQ=255) cccAACTTGGCGCCCAAGAAGATCGCCAGTAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTATTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGc < 2:489437/149‑1 (MQ=255) cAATCTGGCGCCCAAGAAGATCGGCAGCGGGACGTTGATAAGTTCTACAACTCGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCt < 1:492015/149‑1 (MQ=255) aTCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttt < 2:478934/149‑1 (MQ=255) tCTGGCGCGCATGGAGATCGGCTGCAAAACTATGTGAAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCtttt < 2:184502/149‑1 (MQ=255) gcgcCCAAGAATATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAt < 2:303493/149‑1 (MQ=255) tCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTACCTTTTTTATTTA‑TATTACCCTg > 2:157267/1‑149 (MQ=255) cAACACTATGCTGAGTTCTACCACTAGCATATTAGCCTTCCTGGTTGTTTAAAAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAACAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGAtt < 2:168977/149‑1 (MQ=255) cAAAACTTAGATGCGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTTTActttg < 2:135010/149‑5 (MQ=255) aaaaTTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTa < 1:424587/149‑1 (MQ=255) aaaaCTATGATGATTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTa < 1:652618/149‑1 (MQ=255) aaaaCTATGATGAGTTCTACAACTATCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTa < 1:333667/149‑1 (MQ=255) tgatgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTGACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCATGTCATCTGACGTGCCTTTTTTACTTG‑AACTACCCTGTACGATTACTGTTCg < 1:585230/149‑1 (MQ=255) atgtGTTCTACAACTAGCATATTAGTCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACCGCGCACAATAAAAAAGGCACCCCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCt < 2:325325/149‑1 (MQ=255) gaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCttt < 1:751598/149‑1 (MQ=255) aGTTCTACAACGAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTtc < 1:180516/149‑1 (MQ=255) ttCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCACCTTACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTtcat < 2:515038/149‑1 (MQ=255) cTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTtcatca < 1:311110/149‑1 (MQ=255) cAACTAGCATATTAGCTTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTCAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGta < 2:275720/149‑1 (MQ=255) aaCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTTTTTGTTATTACCCTGTGCGATACCTGTTCCCTttcttcgtta > 1:366213/1‑145 (MQ=255) gCATATTAGCCTTCCTTGTTTTTTACCAAGTTGATATCAGATTGTTATATTTAAGCTACTGCTCACAAGTAAAAAGGCACGTTATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGt > 2:350329/1‑149 (MQ=255) gCATATTAGCCTTCCTTGTTTTTAAACAGGTTGAGATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATCGCGt > 1:432652/1‑149 (MQ=255) aGCCTTCCTTGTTGTTTAACAAGTCGATATTAGATTTTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtt < 2:145500/149‑1 (MQ=255) gCCTTTCTTGTTTTTTAACAAGTTGATATTAGTTTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTGTAGCGTTTTGCttt < 1:740188/149‑1 (MQ=255) gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCTTCAGTATAGCGTTTTGCttt > 2:572097/1‑149 (MQ=255) ccttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTCACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTg < 1:657813/149‑1 (MQ=255) cttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCCTATGACGTGCCTTTTTTATTTG‑TACTAACCTGTACGATTCCTGTAGGCTTTGATCAGTCTACCGTTTTGCTTTGTAAGc > 2:154694/1‑149 (MQ=255) gTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCgg < 2:119545/149‑1 (MQ=255) ttttttAACAAGTTGATATTATATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCggg < 1:39743/149‑1 (MQ=255) aaCAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TCCTACCCTGTACGATTACTGTTCGCTTTCATCCGTATAGCGTTTTGCTTTGTAAGCCGTGTGCATc > 2:31753/1‑149 (MQ=255) tatTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTTCATCAGATTCTGtc > 2:446494/1‑148 (MQ=255) tAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACgg > 1:626552/1‑149 (MQ=255) ttAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTc < 1:663372/149‑1 (MQ=255) aGTGACTCCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAg < 2:273409/149‑1 (MQ=255) gTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGt > 1:596782/1‑149 (MQ=255) cTCACAGGAAAAAAGGCACGTCATCTGACGTGCCTTTGTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt < 1:49071/149‑1 (MQ=255) cTCACAAGACAAAAGGCACGTCATCTGACGTGCCTTTTTTATTCG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt < 1:122815/149‑1 (MQ=255) cTCACAAGAAAAAAGGTACCTCATCTTACGTGCCGTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCAGCAGTATAGCGTTTTGCTTTGGAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt < 1:361204/149‑1 (MQ=255) cTCACAAGAAAAAAGGCACGTCGTCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTATGATTACTGTTCGCTTTCATCAGGAGAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTTTACGGAGAAAATATCGTCAAGTTCCGCtt < 1:495340/149‑1 (MQ=255) cTCACAAGAAAAAAGGCACGTCATTTGATGTGAATATTTTACTAT‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt < 1:603600/149‑1 (MQ=255) tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc > 1:579464/1‑149 (MQ=255) tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc > 1:590451/1‑149 (MQ=255) tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTCTACGCACAAAATATCGTCAGGTTCCGcgcc > 2:312134/1‑146 (MQ=255) tCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTGGCTTTGTAAGCCGGGTGCATCAGATTATGTCAGGAGAAAATATCGTCAAGTTACGCTTc > 1:140785/1‑149 (MQ=255) gcacaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTc < 2:310083/148‑1 (MQ=255) cacaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCa < 1:734924/149‑1 (MQ=255) cacaAGAAAAAACGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCa < 1:167258/149‑1 (MQ=255) aataaaaaaGGCACGTCAGATGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc > 1:207397/4‑149 (MQ=255) aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc > 1:107483/1‑149 (MQ=255) aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc > 1:293265/1‑149 (MQ=255) gAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTTCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCaa < 1:734155/149‑1 (MQ=255) aaaaGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTATCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAg < 2:347928/149‑1 (MQ=255) | TTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTG‑TACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAG > NC_000913/4366711‑4366990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |