Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 349,717 T→C 11.8% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,7170TC11.8% 119.3 / 3.7 65intergenic (+145/‑295)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base T (25/29);  new base C (4/5);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAC  >  NC_000913/349570‑349857
                                                                                                                                                    |                                                                                                                                            
tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCATTGAAATTTATGGCGGGTAAGACGGATGGCGTCGCATCCGACAACCGCTGCCTGCTGCGCCGCTGCCGTg                                                                                                                                             >  1:571189/1‑149 (MQ=18)
               gATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTTGCAGGGCTAc                                                                                                                              >  1:586992/1‑149 (MQ=255)
                  tGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                                                           <  2:778774/149‑1 (MQ=255)
                    tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGGCGCTGCCGTGGCTTATCAGGCCTACGGGGt                                                                                                                         >  1:135128/1‑149 (MQ=255)
                       tCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACATGACTTATCAGGCCAACggggagaa                                                                                                                      >  1:51608/1‑145 (MQ=255)
                           cAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGCTGCCTGATGCGTCGCTGACGTGACTTAGAAGGACTACGAGGGACACTGa                                                                                                                  >  1:357472/1‑149 (MQ=14)
                            aGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                                                                 <  2:39369/149‑1 (MQ=255)
                                gATGCCTGATGCGTCGCTGACGGGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGt                                                                                                             <  1:176203/149‑1 (MQ=255)
                                      tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGGACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATACGACAACCGATGCCTGGTGCGCCGCTGGCGTGGCTTGTAAGGCCTACGGGGGGGGCAGAACTGTAGGTCg                                                                                                       >  1:29220/1‑149 (MQ=14)
                                      tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGGCGTGACTTATCAGGCCTACGGGGGGCACTGAAATGTAGGACg                                                                                                       >  2:651878/1‑149 (MQ=255)
                                         tGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAATCGGTGCCTGATGCGCCTCTGACGTGACCGAACAGGCCTACGGGGTGCACTGAACGGTAGGTCGGAt                                                                                                    >  1:419607/1‑149 (MQ=14)
                                         tGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGCCGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGAt                                                                                                    >  1:47579/1‑149 (MQ=255)
                                         cgCGTCGCTGCCGCGACTTAGCAGGCCTACGAGGTGCCCGGAACTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGAt                                                                                                    <  1:55506/148‑1 (MQ=14)
                                             tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATTCGCCGCTGACGCGACTTCTCAGGCCTACGGGGCGCCCTCACCGGTAGGTCGGATAAGa                                                                                                >  1:3570/1‑149 (MQ=11)
                                             tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGGGCACTGAACTGTAGGTCGGATAAGa                                                                                                >  1:355557/1‑149 (MQ=255)
                                               gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACCTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAAGTCGGATAAGACg                                                                                              <  1:560493/149‑1 (MQ=255)
                                                cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTGTCAGGCCTACGGGGGGCACTGAACTGTAGGACGGATAAGACGc                                                                                             >  2:767941/1‑149 (MQ=255)
                                                cTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                                                                                             >  1:487716/1‑149 (MQ=38)
                                                cTGACGCGACTTATAAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCAGCTGCCACACAGAAGTGACTTTTCAGGCCTACGGGGCGCACTGAACTGTAGGTCGGACAAGCCGc                                                                                             >  2:349121/1‑149 (MQ=14)
                                                        aCTGATCCGGCCTACGCGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                                                     <  1:232992/149‑1 (MQ=255)
                                                           tATCGGGCCTACGTGGGGCACTGGACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCa                                                                                  <  2:566255/149‑1 (MQ=255)
                                                           tATCAGGCCTGCGCGGTGCACTGGACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCa                                                                                  <  2:752752/149‑1 (MQ=255)
                                                           tATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCa                                                                                  >  1:759585/1‑149 (MQ=21)
                                                            aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCAt                                                                                 >  1:644807/1‑149 (MQ=255)
                                                             tCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                                                                <  1:103358/149‑1 (MQ=255)
                                                                   cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                                          >  1:716667/1‑149 (MQ=255)
                                                                   cTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACaa                                                                          >  1:594714/1‑149 (MQ=255)
                                                                    tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGCCCCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAAc                                                                         <  1:693038/149‑1 (MQ=255)
                                                                      cGAGGGGCACTGAACTGTAGGTCGGATAAGACGGATGGGGTCGCAACCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCg                                                                       <  2:187151/149‑1 (MQ=255)
                                                                        aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATGCGACAACCGag                                                                     >  1:443770/1‑148 (MQ=38)
                                                                        aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                                     <  2:716667/149‑1 (MQ=17)
                                                                        aGGTGCACTGAACTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt                                                                     <  1:614220/149‑1 (MQ=255)
                                                                         ggTGCACTGGCCTGTAGGTCGGATAAGCCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATg                                                                    <  1:30198/149‑1 (MQ=255)
                                                                            gCACTGTACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt                                                                 <  1:649266/149‑1 (MQ=38)
                                                                            gCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt                                                                 <  1:2511/149‑1 (MQ=37)
                                                                             cACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg                                                                <  1:73986/149‑1 (MQ=38)
                                                                             cACTGAACTGTAGGGCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGTCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCACAGCGTCGCATCCGACAACCGATGCCTg                                                                <  1:109800/149‑1 (MQ=255)
                                                                                tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                                                             >  1:294270/1‑149 (MQ=38)
                                                                                   aCTGTAGGTCGGATAAGAGGGACGGCGTCGCATCCGAACACAAACGCCTATACTAACGCGTACGCGACTTATCACGCCTACGGGGTGCACTTAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                          <  1:343618/149‑1 (MQ=11)
                                                                                     tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                                                        <  1:342638/149‑1 (MQ=37)
                                                                                       tAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAGATGCCTGATGcgccgcg                                                      >  1:627338/1‑148 (MQ=37)
                                                                                       gaGGTCGGAAAAGCGGGGTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCt                                                      <  2:2627/148‑1 (MQ=255)
                                                                                           tCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcg                                                  <  1:243466/149‑1 (MQ=37)
                                                                                           tCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcg                                                  >  2:36734/1‑149 (MQ=38)
                                                                                           ccGGATAAGAGGGAGGGCGTCGCATCCGACACCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTGGCATCCGACAACCGATGCCTGATGCGCCGCTGAcg                                                  <  2:486608/148‑1 (MQ=255)
                                                                                             tgATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                <  1:192274/148‑1 (MQ=38)
                                                                                             ggATAAGCCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACTGATGCCTGATGCGCCGCTGAcgcg                                                <  2:461808/149‑1 (MQ=255)
                                                                                             ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                <  1:543758/149‑1 (MQ=37)
                                                                                             ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                <  1:192218/149‑1 (MQ=37)
                                                                                             ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                <  1:446734/149‑1 (MQ=38)
                                                                                             ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg                                                <  1:446738/149‑1 (MQ=37)
                                                                                              gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACt                                                                                                               >  1:114202/1‑85 (MQ=255)
                                                                                              gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGGCGGATAAGACGCATAGCGTCGCATCCGACAACCAATGCCTGATGCGCCGCttgagcgc                                               >  2:233870/1‑142 (MQ=255)
                                                                                              gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACt                                                                                                               <  2:114202/85‑1 (MQ=18)
                                                                                                tAAGACGGCGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACt                                             <  2:692776/149‑1 (MQ=25)
                                                                                                      ggATTGCGTCGCATCCGACAACCGAGGCCTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATTCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg                                       <  2:183187/149‑1 (MQ=9)
                                                                                                       gctGGCGTCGCATCCCACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg                                      <  2:116708/147‑1 (MQ=18)
                                                                                                               cGCATCCGACAGCCGATGCCTGATGCGTCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                              <  2:779477/149‑1 (MQ=17)
                                                                                                                      gACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACAGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACt                       >  2:501589/1‑149 (MQ=25)
                                                                                                                       acgaCCGATGCCTGATGCTCCGCTGACGGCACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTg                      <  1:279311/146‑1 (MQ=255)
                                                                                                                        cAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGGGCCCtca                     >  1:354230/1‑147 (MQ=12)
                                                                                                                        cAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACGTCTCAGGCCTACGGGGCGCACTGa                     >  2:564188/1‑149 (MQ=255)
                                                                                                                           ccGATGCCGTATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCTCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACt                  <  1:307438/149‑1 (MQ=255)
                                                                                                                               tGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAg              >  1:485507/1‑149 (MQ=35)
                                                                                                                                            cgcTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACCGAACTGTAGGGCGGATaggca  >  1:193393/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
TAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGAC  >  NC_000913/349570‑349857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: