Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 514,150 C→T 14.5% A53V (GCA→GTA)  cueR → copper‑responsive regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913514,1500CT14.5% 101.2 / 7.2 48A53V (GCA→GTA) cueRcopper‑responsive regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (2/5);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCAT  >  NC_000913/514002‑514294
                                                                                                                                                     |                                                                                                                                                
aGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGc                                                                                                                                                  >  2:410828/1‑149 (MQ=255)
aGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGc                                                                                                                                                  >  1:94036/1‑149 (MQ=255)
aGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGATGGTGAGGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCGACACGCAGCAGAATCTCAACGAACGTGCCTGACT‑GCGCCTGGc                                                                                                                                                  >  2:483236/1‑149 (MQ=255)
       tAGCAAAACTTCCCGGCCTGACCAGCAACGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGCTGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGTACGGCAg                                                                                                                                           <  2:594345/149‑1 (MQ=255)
       tAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGTACGGCAg                                                                                                                                           <  1:43837/149‑1 (MQ=255)
       caGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGTACGGCAg                                                                                                                                           <  1:226207/148‑1 (MQ=255)
         gCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGCCGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑CCGCCAGGCACGGCAGGt                                                                                                                                         >  1:66669/1‑149 (MQ=255)
         gCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACCGACCTTACT‑GCGCCAGGCACGGCAggc                                                                                                                                         >  1:670012/1‑148 (MQ=255)
               agtACCGGCCTGACCAGCAAAGCCATTCGCTCCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACCGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCtt                                                                                                                                   <  1:59247/147‑1 (MQ=255)
               aTTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCtt                                                                                                                                   <  1:567358/149‑1 (MQ=255)
                            ccAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAagag                                                                                                                      >  2:434221/1‑149 (MQ=255)
                                cAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCCCGGCAGGTGGGCCTTAACCTGGAAGAGAgcg                                                                                                                  >  1:142368/1‑149 (MQ=255)
                                cAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAgcg                                                                                                                  >  2:756867/1‑149 (MQ=255)
                                cAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAgcg                                                                                                                  >  1:291114/1‑149 (MQ=255)
                                cAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAgcg                                                                                                                  >  1:312267/1‑149 (MQ=255)
                                    gCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCATCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGa                                                                                                              <  2:536711/149‑1 (MQ=255)
                                       cttCGCTCTTATGAAGAGAAGGGGCTGGTGACGCCGCCGACGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCt                                                                                                           <  1:208508/148‑1 (MQ=255)
                                            cTTCAATGAAGCGAAGGGGCTGGTGACGCCGCCGATGCGTATCCAAAACGGTTATCGCACCTACACGCAGCAGAATCTCAGCGAACTGACCTTACT‑GCGCCCGGCACGGCAGGTGGGCTTTAACCCGGAAGAGAGCGGCGAGCTGGTGa                                                                                                      <  2:348980/149‑1 (MQ=255)
                                                tATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGATCGGCGAGCTGGTGAATCt                                                                                                  <  1:574442/149‑1 (MQ=255)
                                                  tGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGt                                                                                                >  1:598830/1‑149 (MQ=255)
                                                      gagaAGGGGCTGGTGACGCCGCCGACGCGCAGCGAAAACGGCTATCGCATCTACACGCAGCATCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGTAGAGAGAGGCGAGCTGGTGAATCTGTTTaa                                                                                            <  2:537472/149‑1 (MQ=255)
                                                         aaGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGTACGGCAGCTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGa                                                                                         <  1:560359/149‑1 (MQ=255)
                                                              gCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGCGCTGGTGAATCTGTTTAACGACCCGc                                                                                    >  1:44421/1‑149 (MQ=255)
                                                                   tGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGGAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCgg                                                                               >  2:477141/1‑149 (MQ=255)
                                                                                gcgcAGCGAAAACGGCTATCGCGCCTACACGCAGCAGCATCTCAACGAACTGACCTTAAT‑GCGACAGGTACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGTCGCGCTGGGGAATCTGGTTAACGACCCGCAGCGGCCCGGCGCCAACg                                                                  >  2:145448/1‑149 (MQ=255)
                                                                                         aagcGGTTATCGCACCCACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGAACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGgc                                                         <  2:670012/146‑1 (MQ=255)
                                                                                         aaaCGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGgc                                                         <  1:491900/149‑1 (MQ=255)
                                                                                         aaaCGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGgc                                                         <  2:66669/149‑1 (MQ=255)
                                                                                         aaaCGGGTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTGCT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGATTCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGgc                                                         <  2:221264/149‑1 (MQ=255)
                                                                                          accGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCCCGGCAGGGGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGgcg                                                        <  2:669213/147‑1 (MQ=255)
                                                                                          aaCGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCGGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCCAACGgcg                                                        >  2:531238/1‑149 (MQ=255)
                                                                                               ttATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCTAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGc                                                   <  2:433366/149‑1 (MQ=255)
                                                                                                 aTCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTg                                                 <  1:568210/149‑1 (MQ=255)
                                                                                                 aTCGCACCTACACGCAGCAGCATCGCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTg                                                 <  2:111652/149‑1 (MQ=255)
                                                                                                 aTCGCACCCACACGCACCAGCATCCAAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTTGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGTTACAGCGCCGACGTCAAACGGCGCACGCTg                                                 <  2:437595/149‑1 (MQ=255)
                                                                                                  tCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGTACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTgg                                                >  2:722855/1‑149 (MQ=255)
                                                                                                   cGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGga                                               <  1:449985/149‑1 (MQ=255)
                                                                                                         tACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGt                                         >  1:21076/1‑149 (MQ=255)
                                                                                                           gtgccagcagcaTCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCATTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGCGAAGGTgg                                       >  2:500343/5‑149 (MQ=255)
                                                                                                           cacGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTGTAACCGGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCAGGAGAaggagg                                       >  2:714032/1‑149 (MQ=255)
                                                                                                            acGCAGCAGCATCTCAACGAACTGACC‑TACTGGCCTCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCCCAGCGCCGACGTCAAACGGCGCACGCGGGGGAAGggggg                                      >  2:363905/1‑148 (MQ=255)
                                                                                                               cagcagcaTCTCAACAAACTGCACTTACT‑GCGCCGTCTACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTTTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGga                                   <  1:205186/149‑1 (MQ=255)
                                                                                                                agcagcaTCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCgggg                                  >  1:377565/1‑147 (MQ=255)
                                                                                                                   agcaTCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCCCCGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGGGGCGGAGGTc                               >  1:95654/1‑149 (MQ=255)
                                                                                                                      aTCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGaa                            <  2:21076/149‑1 (MQ=255)
                                                                                                                       tctcAACGAACTGGCCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAAc                           <  2:672265/149‑1 (MQ=255)
                                                                                                                           aaCGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGGGAGCGAACGaca                       >  1:281525/1‑149 (MQ=255)
                                                                                                                                              cgcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATc     >  1:360689/1‑149 (MQ=255)
                                                                                                                                               gcCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGCGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATcc    <  2:598830/149‑1 (MQ=255)
                                                                                                                                                ccAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGACCGACACATTGAGGAGCTGCAATCCa   >  2:137973/1‑149 (MQ=255)
                                                                                                                                                 cAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGCGAAGGTGGCGGAGATAGAACGACACATTGAGGAGCTGCAATccgt  >  2:92250/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
AGCGATGTAGCAAAAATTACCGGCCTGACCAGCAAAGCCATTCGCTTCTATGAAGAGAAGGGGCTGGTGACGCCGCCGATGCGCAGCGAAAACGGTTATCGCACCTACACGCAGCAGCATCTCAACGAACTGACCTTACT‑GCGCCAGGCACGGCAGGTGGGCTTTAACCTGGAAGAGAGCGGCGAGCTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCAT  >  NC_000913/514002‑514294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑