Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 60 [0] [0] 61 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)57 (0.710) 42/280 1.5 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913/257761‑258053
                                                                                                                                                  |                                                                                                                                                  
gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                  <  1:1063999‑M1/149‑3 (MQ=255)
gCTTGTGTTAAAAATTTGCTAAATTTTGCCAAGTTGGTAAAACAGTTGCATTACAACAGGGGATAGCAATGACGTTACCGAGTGGACACTCGAAGTGAAGATTAAAAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                  <  2:1385617‑M1/149‑3 (MQ=255)
  ttGTGCTAAAAATTTTCTAAATTTTGTCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtg                                                                                                                                                <  2:1255554‑M1/149‑5 (MQ=255)
        tAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaga                                                                                                                                          <  2:884462‑M1/149‑11 (MQ=255)
         gaaaatGTGCTAGATTGTGCCAATTTGGTAAAACAGTTGCATCACAACAGGGGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagat                                                                                                                                         <  2:267531‑M1/148‑12 (MQ=255)
            aagttgctAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                      <  2:316552‑M1/146‑15 (MQ=255)
            aaTTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                      <  1:798489‑M1/149‑15 (MQ=255)
             aTTTGCCAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGCTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                     <  1:1154820‑M1/149‑16 (MQ=255)
                 gCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgatt                                                                                                                                 <  2:858231‑M1/149‑20 (MQ=255)
                  cTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                                >  2:928362‑M1/1‑129 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCACTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagctaatcgaatct                                                                                                                               >  2:349418‑M1/1‑128 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                               >  1:925838‑M1/1‑128 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatagattat                                                                                                                               >  2:1390321‑M1/1‑128 (MQ=255)
                     aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgatccttt                                                                                                                             >  2:607161‑M1/1‑126 (MQ=255)
                       ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGCCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                           <  1:1224337‑M1/149‑26 (MQ=255)
                       ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                           >  2:393143‑M1/1‑124 (MQ=255)
                       ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                           >  1:109666‑M1/1‑124 (MQ=255)
                       tttcGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                           <  1:519505‑M1/149‑26 (MQ=255)
                          tGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                        >  2:658696‑M1/1‑121 (MQ=255)
                               aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATTACGTTACCGAGTGGACGCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                                   >  2:301900‑M1/1‑116 (MQ=255)
                               aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAAGTAaatgcacagataatcgattctttttctcttgtt                                                                                                                   >  1:1357223‑M1/1‑116 (MQ=255)
                                          cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAGGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                        <  1:1309897‑M1/149‑45 (MQ=255)
                                            ggtgcATCACAACAAGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgt                                                                                                      <  1:1055889‑M1/147‑47 (MQ=255)
                                             ttGCATCACAACAGGAGATAGCAATGACGTTACCGGGTGGACACCCGCAGAGGAGAGTGATCAAAAAATTTACGGCACTAGGCCCGTATATTCGTGACGGTAagtgcaaagatcatcgattctttttcgattgtctggctgtaggcgtc                                                                                                     >  2:6852‑M1/1‑102 (MQ=255)
                                                cATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                                  <  1:393959‑M1/149‑51 (MQ=255)
                                                 aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTGGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattgtttttcgattgtctggctgtatgcgtcaacg                                                                                                 >  1:192610‑M1/1‑98 (MQ=255)
                                                 aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                 <  1:674040‑M1/149‑52 (MQ=255)
                                                  tCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgt                                                                                                >  1:571550‑M1/1‑97 (MQ=255)
                                                     caacaGGAGATAGCAATGTCGTTACCGAGTGGACACCCGACGAGCAGATATACCAAAAAATTTACGGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctctttcgattgtctggcggtatgcgtcaacgtgaa                                                                                             >  1:1023770‑M1/1‑94 (MQ=255)
                                                                tgacacgacgtgaCCGAGTGGACACCCTAAGAGCAGATTGATCAAAAAATTTACCGCACTGGGCCCGTATATTCGTGAAGGTAagtgcaaagataagcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccgg                                                                                  <  2:493658‑M1/143‑67 (MQ=255)
                                                                   aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcggttgtctggctgtatgccgcaacgggaaacgggcaccggaag                                                                               >  2:1274244‑M1/1‑80 (MQ=255)
                                                                    aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                              <  1:658696‑M1/149‑71 (MQ=255)
                                                                       aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcg                                                                           <  1:928362‑M1/149‑74 (MQ=255)
                                                                         gTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgcaaccggcaccggaagtgcgtg                                                                         >  2:530445‑M1/1‑74 (MQ=255)
                                                                          ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtga                                                                        <  2:1249706‑M1/149‑77 (MQ=255)
                                                                                 gTGGACACCCGAAGAGCAGATTGATCAAACAATTTACCGCACTAGGCCCGTATATACGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                 <  2:815164‑M1/149‑84 (MQ=255)
                                                                                 gTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                 <  1:791424‑M1/149‑84 (MQ=255)
                                                                                 gTGGACACCCGAAGAGCAGATTGAGCAAAAAATTTACCGCACTCGGCCCGTATATTCGTGAAGGTAagtgcaaagattatcgattctttttctattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgagttctgg                                                                 <  2:902888‑M1/149‑84 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                           <  1:1368663‑M1/149‑90 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgggagttctgggggcgg                                                           >  2:30658‑M1/1‑60 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATAGTCGTGAAGGTAagtgcaacgataatcgattctgtttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                           >  2:352560‑M1/1‑60 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                         >  2:800298‑M1/1‑58 (MQ=255)
                                                                                            aaGAGCAGAATGAACAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggtagtgcgtgaattctggggctgttggat                                                      <  2:1166750‑M1/149‑95 (MQ=255)
                                                                                                gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggag                                                  >  1:918276‑M1/1‑51 (MQ=255)
                                                                                                 cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagc                                                 >  1:484045‑M1/1‑50 (MQ=255)
                                                                                                 cAGATTGATCAAAAAATTTAACGAACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatggtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaattctggggccgggggatggagc                                                 >  2:328882‑M1/1‑50 (MQ=255)
                                                                                                   gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccaacaccggaagtgcgtgaattctggggctggtggatggagctg                                               >  2:639021‑M1/1‑48 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCGGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                       >  1:247226‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtgaacgtgaaaccggcaccggaagtgggtgaattctggggctggtggatggagcaggaagggcc                                       >  1:249504‑M1/1‑40 (MQ=255)
                                                                                                                aTTTACCTCACTCGGCCCGTATATTCGTGAAGGTAcgtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat                                  <  2:1023770‑M1/149‑115 (MQ=255)
                                                                                                                aTTTACCCCACCAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaat                                  <  1:1276722‑M1/149‑115 (MQ=255)
                                                                                                                          cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctgcttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                        <  1:357623‑M1/149‑125 (MQ=255)
                                                                                                                               cccGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttgcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                   >  1:680778‑M1/1‑20 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacgg                  >  2:586926‑M1/1‑19 (MQ=255)
                                                                                                                                    atatCCGGGAAGGTAagtgcaaagatattcgattctttttcgagtgtcgggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac              <  1:62688‑M1/149‑135 (MQ=255)
                                                                                                                                          gTGAATGTAagtgcaaagataatcgattcgttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt        <  2:210983‑M1/149‑141 (MQ=255)
                                                                                                                                          gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaagtctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttt        <  1:146811‑M1/149‑141 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt     <  2:571550‑M1/149‑144 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttccattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt     <  1:71923‑M1/149‑144 (MQ=255)
                                                                                                                                                gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg  >  1:296850‑M1/1‑3 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913/257761‑258053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.