Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,413,542 A→T Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,413,5420AT98.9% 301.6 / NA 88Y67N (TAT→AAT) ydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (40/47);  minor base G (0/1);  total (40/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413397‑1413687
                                                                                                                                                 |                                                                                                                                                 
cGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCaa                                                                                                                                                <  1:1250018/149‑1 (MQ=255)
   aGCATCAGGCCGTATTCAACCATCCACCCTTCATTGAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTCTAATTTGAATTCAATGc                                                                                                                                             >  2:840153/1‑149 (MQ=255)
    gCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTACTTTGAATTCAATGCa                                                                                                                                            >  1:1193621/1‑149 (MQ=255)
    gCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCa                                                                                                                                            >  2:1096412/1‑149 (MQ=255)
     cATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATTATCAGCTATGAAGTTTTAATTTGAATTCAATGCaa                                                                                                                                           <  1:1166176/149‑1 (MQ=255)
     cATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATAACCTATGAAGTTTTAATTTGAATTCAATGCaa                                                                                                                                           >  2:784411/1‑149 (MQ=255)
            ccGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGa                                                                                                                                    <  1:1149322/149‑1 (MQ=255)
            ccGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGa                                                                                                                                    <  1:1055282/149‑1 (MQ=255)
                    aaCCATCCAGTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            <  2:146239/149‑1 (MQ=255)
                    aaCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGaa                                                                                                                            >  1:597445/1‑149 (MQ=255)
                     aCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGCTGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAg                                                                                                                           >  1:614707/1‑149 (MQ=255)
                     aCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAg                                                                                                                           <  2:439434/149‑1 (MQ=255)
                     aCCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAg                                                                                                                           <  1:397916/149‑1 (MQ=255)
                       cATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGtt                                                                                                                         >  2:600337/1‑149 (MQ=255)
                        acccACTCTTCATCAAAGATTACTTGTGCCATCGCATCACCCCTGCCAGGCGCCAATGTTAGACTGAAATTCACGCCCGATGATGATTATTAATAATCAGCTATGAAGTCTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGttt                                                                                                                        <  2:698262/147‑1 (MQ=255)
                         tCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCATTGCAATTCTCGAGGACTGAAGTTtc                                                                                                                       <  1:700717/149‑1 (MQ=255)
                           cACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtctc                                                                                                                     <  1:1287608/149‑1 (MQ=255)
                           cACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtctc                                                                                                                     >  2:594109/1‑149 (MQ=255)
                           cACTCTTCATTAAACATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTtctc                                                                                                                     <  1:136103/149‑1 (MQ=255)
                            aCTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCg                                                                                                                    >  2:530493/1‑149 (MQ=255)
                              tctTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGGAGTTTCTCGCa                                                                                                                  >  2:756430/1‑149 (MQ=255)
                                ctCATTAAAGATTACTTGTGCCATCTCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTAACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAAt                                                                                                                <  2:1010498/148‑1 (MQ=255)
                                  cATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTa                                                                                                              <  2:1275541/149‑1 (MQ=255)
                                    ttAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTaaa                                                                                                            >  1:143209/1‑149 (MQ=255)
                                        aGATTACTTGTGCCATCGCATCACCTCCGGCAGGCGCCAATGTTAGACTGAAATGGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAAttc                                                                                                        >  1:200645/1‑148 (MQ=255)
                                          aTTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTAt                                                                                                      >  1:36264/1‑149 (MQ=255)
                                          aTTACGTGTGCCATCGCATCACCTCTGGCCGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTCAt                                                                                                      <  2:212391/149‑1 (MQ=255)
                                           ttACTTGTGCCATCGTATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCACAATGTTAATTATTCATAATCAGCTATGAAGATTTAATTTAAATTAACTGCAATTTTCGAGAACTGACGTATCTAGCAATTAAAATTTAta                                                                                                     >  2:76432/1‑148 (MQ=255)
                                              cTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAGTCAGCTATGAAGTTTTAATTTGAATTCAATTCAATTCTCGAGGATTGTAGTTTCTCTCATTTGAATTTTATCGGt                                                                                                  >  1:487435/1‑149 (MQ=255)
                                               ttGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtt                                                                                                 >  2:1285342/1‑149 (MQ=255)
                                               tcgttccATTGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtt                                                                                                 <  2:1360702/144‑1 (MQ=255)
                                                tgtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGttt                                                                                                <  1:304273/149‑1 (MQ=255)
                                                tgtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGttt                                                                                                <  1:631488/149‑1 (MQ=255)
                                                 gtgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGtttt                                                                                               >  2:234359/1‑149 (MQ=255)
                                                  tgCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTa                                                                                              >  1:1345456/1‑149 (MQ=255)
                                                    ccATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACt                                                                                            >  1:372202/1‑149 (MQ=255)
                                                    ccATCGCATCACCTCGGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACt                                                                                            <  1:1065376/149‑1 (MQ=255)
                                                             cACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGACGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctct                                                                                   <  2:720974/149‑1 (MQ=255)
                                                              aCCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctc                                                                                  <  1:1327144/149‑1 (MQ=255)
                                                               ccTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGCATTCCATGCCATTCTCGAGGCCTGAAGTTTCTCGAAATTACAACTTACCAGTTTTACTTTCTGctctct                                                                                 <  2:1027415/149‑1 (MQ=255)
                                                               ccTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctctct                                                                                 <  1:1005693/149‑1 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                <  2:1355557/149‑1 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                <  1:1005796/149‑1 (MQ=255)
                                                                ctctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                <  1:530493/149‑1 (MQ=255)
                                                                  ctGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGa                                                                              <  1:83629/149‑1 (MQ=255)
                                                                     gCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAAc                                                                           >  2:469677/1‑149 (MQ=255)
                                                                     gCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAAc                                                                           >  1:738706/1‑149 (MQ=255)
                                                                       aGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGc                                                                         <  1:1096412/149‑1 (MQ=255)
                                                                             cAATGCTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTCTTGCACTCTGGATAAGCCTGctt                                                                   >  2:976425/1‑149 (MQ=255)
                                                                              aaTGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCCCAATTAGAAGGTGGCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGcttc                                                                  >  1:108230/1‑149 (MQ=255)
                                                                                 gTTAGACTGAAATTGACGCCCGATGTTTATTATTAAGAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCttt                                                               <  2:1119894/149‑1 (MQ=255)
                                                                                      aCTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTAc                                                          >  2:14664/1‑149 (MQ=255)
                                                                                      aCTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTAc                                                          >  2:611352/1‑149 (MQ=255)
                                                                                       cTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTAcc                                                         <  1:146151/149‑1 (MQ=255)
                                                                                          taaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGCAGCTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                      <  2:636833/148‑1 (MQ=255)
                                                                                          aaaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                      <  2:1113428/149‑1 (MQ=255)
                                                                                          aaaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTga                                                      <  2:247398/149‑1 (MQ=255)
                                                                                               gACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGATGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTACCTGAGAGCa                                                 <  2:361263/149‑1 (MQ=255)
                                                                                                  gCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCGCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttt                                              >  1:133026/1‑149 (MQ=255)
                                                                                                    ccGATGTTGATTATTAATAATCAGCTATGAAGTTTTACTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCAttttt                                            >  1:931976/1‑149 (MQ=255)
                                                                                                      gATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTc                                          <  2:757331/149‑1 (MQ=255)
                                                                                                       aTGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCg                                         <  2:36264/149‑1 (MQ=255)
                                                                                                        ggTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGc                                        <  2:442604/148‑1 (MQ=255)
                                                                                                          ttGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCCATGCAATTCTCGAGGAATGTAGATTATCGCAATTAAAATTGATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCgact                                      >  2:1374580/1‑146 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  2:334159/1‑149 (MQ=255)
                                                                                                            gATTATTAATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTc                                    >  1:1363410/1‑149 (MQ=255)
                                                                                                             attattAATAATCGGCGATGAAGTCTTAATTTGAATTCCATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCt                                   <  2:1193621/149‑1 (MQ=255)
                                                                                                              ttattaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTGTTTACATGAGAGCATTTTTTTGCATTCTg                                  >  1:518616/1‑149 (MQ=255)
                                                                                                                 ttaATAATCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAtt                               >  1:479258/1‑149 (MQ=255)
                                                                                                                  taataaTCAGCTATGAAGTTTTACTTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTTAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAttt                              >  2:899542/1‑149 (MQ=255)
                                                                                                                   aataatCAGTTACTAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCCGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTTTTTTTTACCTGTGAGCATTTTTTCGCATTCTGATTTc                             <  2:396373/149‑1 (MQ=255)
                                                                                                                   aataatCAGCTATGAAGTTTTAATTTGAATTCCATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTc                             <  1:1124989/149‑1 (MQ=255)
                                                                                                                   aataatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTc                             >  1:1153028/1‑149 (MQ=255)
                                                                                                                     taatCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGt                           <  1:141027/149‑1 (MQ=255)
                                                                                                                        tCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAg                        >  1:1199113/1‑149 (MQ=255)
                                                                                                                        tCAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAg                        <  1:721428/149‑1 (MQ=255)
                                                                                                                         cAGCTATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGTAAACGCCTGCTTCTTTTTTACCTGTGAGCATTTTTTCGCATTCTGTTATCGTTact                       <  2:366606/149‑3 (MQ=255)
                                                                                                                          aGCTATGTAGTTTTAACTTGAATGCAATGCAATCCACGAGGACTTGAGTTTCTCGCAATTAAGATCGAGCAGTTTTACTTTCTGCTCTCTGGAGTCGCCTGCTCCTTTTTTGCCTTAGTGCATTTTTTCGCATTCTGATTTCGTTAttt                      <  2:379413/149‑1 (MQ=255)
                                                                                                                             tATGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTCAAGATTCTCGCAATTAAACTTGATCCGTTTTACTTTCTGCTCGCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGAATTCGTTATTTTAg                   >  1:740533/1‑149 (MQ=255)
                                                                                                                               tGAAGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCACAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAt                 >  1:684704/1‑149 (MQ=255)
                                                                                                                                 aaGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTTAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAttt               <  1:1390996/149‑1 (MQ=255)
                                                                                                                                 aaGTTTTAATTTGAATTCAATGCAATTCTCGAAGACTGAAGTTTCTCTCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCCCATTCTGATTTCGTTAGTTTAGAttt               <  2:487435/149‑1 (MQ=255)
                                                                                                                                  aGTTTTAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGAtttt              <  2:597445/149‑1 (MQ=255)
                                                                                                                                      ttAATTTGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTTAat          >  2:1078576/1‑149 (MQ=255)
                                                                                                                                          tttGAATTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCt      >  2:1210874/1‑149 (MQ=255)
                                                                                                                                           ttGAATTCTATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCtt     <  1:600337/149‑1 (MQ=255)
                                                                                                                                              aaTTCAATGGCATTCTCGAGGACTAAAGTTTCTCGTATTTATAATTTATCCGTTTTACTTTCTGCTCTCTGGAAGCGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCAATCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTc  <  1:1178279/149‑1 (MQ=255)
                                                                                                                                              aaTTCAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTATACGTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTc  >  2:578535/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGAAGCATCAGGCCGTATTCAACCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTC  >  NC_000913/1413397‑1413687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: