Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA97.7% 260.2 / NA 86A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (34/49);  minor base T (0/2);  total (34/52)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAA  >  NC_000913/2092895‑2093181
                                                                                                                                                 |                                                                                                                                             
ggATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtt                                                                                                                                            <  2:861818/149‑1 (MQ=255)
 gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                           >  1:203395/1‑149 (MQ=255)
 gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCCGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                           >  2:1269640/1‑149 (MQ=255)
   ttttCGCACCCTGCTGGAGTTAACCCGGGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTg                                                                                                                                         <  2:187239/149‑1 (MQ=255)
      tCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTAAATCTTCAAGCCCATACCGGTGCTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTGTTTTACGCGCACTGTCGAAAACTTTTGctc                                                                                                                                      >  1:1373442/1‑149 (MQ=255)
        gCACCCTGTTGCAGTTCACCCGCGGTAAGGCGTTCGTGGTTGCCGATGAAGCCCATATCGAGTTTCGCCCGCAGGCCTCGCTGGCTGGCTGGCTGGCGTAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAATCTTTTGCTCTg                                                                                                                                    <  1:216367/149‑1 (MQ=255)
         cACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGGCGAAAACTTTTGCCCTgg                                                                                                                                   >  2:603337/1‑149 (MQ=255)
          gcccGGCGGGAGTTACCCCGCGGTAAGGCGATGGTGGGTGCCGATGAAGCGTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCGCGTTTCTATTTTACGCACACTGTCGAAAACTTTTGTTCTGGc                                                                                                                                  <  2:925260/148‑1 (MQ=255)
                 tgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCCCTGGCGGGGCtt                                                                                                                           >  1:500148/1‑149 (MQ=255)
                  ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCAACGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGTCTAAAACTTTTGCTCTGGCGGGGCTTc                                                                                                                          >  1:857583/1‑149 (MQ=255)
                     gTTAACCCGCGGTAAGGCGATGGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGTCGGGGCTTCGtt                                                                                                                       <  2:167751/149‑1 (MQ=255)
                       tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                     <  2:1223012/149‑1 (MQ=255)
                        aaCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCg                                                                                                                    >  1:1241757/1‑149 (MQ=255)
                         aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATAGCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                   <  1:226737/149‑1 (MQ=255)
                          cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                  <  2:381157/149‑1 (MQ=255)
                            cgcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAtt                                                                                                                >  2:718781/1‑149 (MQ=255)
                             gcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAttt                                                                                                               <  1:1324850/149‑1 (MQ=255)
                               ggTAAGGCGATTGTGGTTGCCGATGATGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                             <  1:121245/149‑1 (MQ=255)
                               ggTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAc                                                                                                             <  1:521947/149‑1 (MQ=255)
                                     gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGc                                                                                                       <  1:873382/149‑1 (MQ=255)
                                            tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                <  2:520055/149‑1 (MQ=255)
                                            tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                >  1:306813/1‑149 (MQ=255)
                                            tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                <  1:1033999/149‑1 (MQ=255)
                                             ggTTGGCGGTGAACCCTATAGCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                               <  2:393044/149‑1 (MQ=255)
                                             ggTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                               <  1:1000772/149‑1 (MQ=255)
                                              gTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTAGGCTGCGAAACgaaga                                                                                              >  2:219427/1‑149 (MQ=255)
                                               ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                             <  2:2303/149‑1 (MQ=255)
                                               ttGCCGATGAAGCCTATACCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                             <  2:995162/149‑1 (MQ=255)
                                               tcgCCGATGACGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTAGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGTCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                             <  2:813640/147‑1 (MQ=255)
                                                  ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtc                                                                                          <  2:1004605/149‑1 (MQ=255)
                                                   cgagcaaGCCTATATCGGGTCTCGACCGCCCGCCTGGCTGGCTGGCTCGCTGGCGGAATATCCGCACCTGGTTATTTTCCGCACACTGTCGAAATCTGTTGCTCTGGCGGGGCCTCGTTGCGGATTTACGCTGGCAAACGAAGCAGtca                                                                                         <  2:178005/144‑1 (MQ=255)
                                                     gtGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                       <  2:778166/148‑1 (MQ=255)
                                                        aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACCTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                    <  1:904547/149‑1 (MQ=255)
                                                        aaGCCTATATCGAGGTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAc                                                                                    <  1:625208/149‑1 (MQ=255)
                                                            ccatatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                <  1:370739/147‑1 (MQ=255)
                                                            cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                >  2:1034532/1‑149 (MQ=255)
                                                               tatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCAACAACCTGCtga                                                                             >  2:562193/1‑149 (MQ=255)
                                                                atCGAGTTTTGCCAGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgat                                                                            <  2:620853/149‑1 (MQ=255)
                                                                 tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatg                                                                           >  1:164113/1‑149 (MQ=255)
                                                                   gAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaa                                                                         >  2:659664/1‑149 (MQ=255)
                                                                       tttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCGGGTGAAAGTg                                                                     >  2:264350/1‑149 (MQ=255)
                                                                        ttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGa                                                                    <  1:718781/149‑1 (MQ=255)
                                                                           cccGCAGGCATCGCTGGCTGGCTGGATGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACTTGCCGATGAAAGTGATCg                                                                 <  1:1027110/149‑1 (MQ=255)
                                                                                  gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGAGGCTATACTCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                          >  2:79977/1‑149 (MQ=255)
                                                                                  gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                          >  1:1096651/1‑149 (MQ=255)
                                                                                   cATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCCAACGAAGAAGTCATCAACCCGCTGATGAAAGTGATCGCCCCCTAc                                                         >  2:713062/1‑149 (MQ=255)
                                                                                    aTCGCGGGCTGGCTGGCGGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                        <  1:372266/149‑1 (MQ=255)
                                                                                      cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                      <  1:1344046/149‑1 (MQ=255)
                                                                                      cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                      <  1:505711/149‑1 (MQ=255)
                                                                                           gctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCtctc                                                 <  2:306813/149‑1 (MQ=255)
                                                                                            ctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGAtctcc                                                >  1:1096866/1‑148 (MQ=255)
                                                                                             tggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCACCCTACCCGCTCTCGa                                               >  1:447008/1‑149 (MQ=255)
                                                                                             tggccggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                               <  1:1269640/149‑1 (MQ=255)
                                                                                                ctggctgtcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                            <  1:695443/149‑1 (MQ=255)
                                                                                                  ggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                          <  2:647300/149‑1 (MQ=255)
                                                                                                  ggctggcGAAATATCCGCACCTGGCTATTATACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTTCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                          <  2:886830/149‑1 (MQ=255)
                                                                                                     tggcGGAATCTGCGCGTGTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCGCTGGCGGGGCGTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCAGCAACCGGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                       <  2:1302815/149‑1 (MQ=255)
                                                                                                     tggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGtt                                       >  1:977771/1‑149 (MQ=255)
                                                                                                       gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCCACCCGCTCTCGACGCCGGTTGc                                     >  2:727305/1‑149 (MQ=255)
                                                                                                          gAATATCCGCACCTGGGTATTTTACGCACACTGGCGAAAACTTGTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGa                                  <  2:102256/149‑1 (MQ=255)
                                                                                                                 cccACCGGGCTATTTTACGCACACTGTCGAAAACATTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCCGGCAAACGAAGAAGTCATCAACCTGCTTATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCg                           <  2:665159/147‑1 (MQ=255)
                                                                                                                  gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCACCCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCgg                          <  2:1076180/149‑1 (MQ=255)
                                                                                                                  gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCGCCCTACCCGCTCTGGAGGCCGGTTGCCGGCATTGCgg                          >  2:433562/1‑149 (MQ=255)
                                                                                                                  gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGAGGAAAGTGATCGCCCCCTACCCGCTCTCGAAGCCGGTTGCCGACATTGCgg                          >  2:790738/1‑149 (MQ=255)
                                                                                                                    aCCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGcc                        <  1:659664/149‑1 (MQ=255)
                                                                                                                      cTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACAAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTCCGGCCCa                      >  2:16974/1‑149 (MQ=255)
                                                                                                                        ggCTATTTTACGCACACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAgg                    <  2:371031/149‑1 (MQ=255)
                                                                                                                         gCTTTTTTACGCAGACCGTCGAACACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTGACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                   <  2:803645/149‑1 (MQ=255)
                                                                                                                         gCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                   <  2:1297761/149‑1 (MQ=255)
                                                                                                                         gCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                   >  1:178562/1‑149 (MQ=255)
                                                                                                                             ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa               >  1:120520/1‑149 (MQ=255)
                                                                                                                             ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa               >  1:752395/1‑149 (MQ=255)
                                                                                                                              tttCCGTACAGTGTCGAAAACTTTTGCTCGGGGGGGGTTTGGTTGCGGATTGAGTGTGGCTAAGGTAGAAGTCATCAACCTGCTGTGGATAGTGATCGCCCCCGTCCGCCTTTCGACTCCGGTTGCCGACATTGTGGCCCAGGCGttta              <  2:456141/149‑3 (MQ=255)
                                                                                                                                tACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACAAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGGTAAGc            >  1:125961/1‑149 (MQ=255)
                                                                                                                                   gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCCTCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCCGGCGTTAAGcccc         >  1:478241/1‑148 (MQ=255)
                                                                                                                                   gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca         <  1:1123590/149‑1 (MQ=255)
                                                                                                                                    cacaCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcac        <  2:164113/149‑1 (MQ=255)
                                                                                                                                    cacaCTGTCGAAAACTTTTGCTCTGGCGGGGCTGCGGTGCGGGGTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcac        <  1:871772/149‑1 (MQ=255)
                                                                                                                                     acacTGTCGAAAACTTTTGCTTTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca       <  1:673609/149‑1 (MQ=255)
                                                                                                                                     acacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAATCGAAGAAGTACTCAACCTGATGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCCGCCCAGGCGGTAGGCCcaca       >  1:1233215/1‑149 (MQ=255)
                                                                                                                                     acacTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCGTACACGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcaca       <  1:886160/149‑1 (MQ=255)
                                                                                                                                       acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAgt     >  1:260705/1‑148 (MQ=255)
                                                                                                                                       acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAATGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAgg     >  1:672856/1‑149 (MQ=255)
                                                                                                                                         tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAACGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCTACATTGCGGCCCAGGCGTTAAGCCCACAGGGa   <  2:1238409/149‑1 (MQ=255)
                                                                                                                                         tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTACGCCCACAGGGa   >  1:18748/1‑149 (MQ=255)
                                                                                                                                          gTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGaa  <  2:943634/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAA  >  NC_000913/2092895‑2093181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: