Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503	1979278	776	45 [0]	[0] 46	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	41 (0.900)	35/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913/1978357‑1978648                                                                                                                                                  |                                                                                                                                                   aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                                 <  1:512660‑M1/149‑4 (MQ=255)     gggTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGGTCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                                                                             <  2:124573‑M1/149‑8 (MQ=255)         cTTTACACTTATCGAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcata                                                                                                                                         <  1:509180‑M1/149‑12 (MQ=255)          tttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatac                                                                                                                                        >  1:337939‑M1/1‑137 (MQ=255)           ttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACGACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                                                                       <  1:580267‑M1/149‑14 (MQ=255)             acacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttt                                                                                                                                     <  1:659234‑M1/149‑16 (MQ=255)             acacTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttt                                                                                                                                     <  2:26491‑M1/149‑16 (MQ=255)               acTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                                                                   >  1:457104‑M1/1‑132 (MQ=255)                       aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAATTAACATAAATGtataagtcatacttttgttttgtgt                                                                                                                           >  1:10451‑M1/1‑124 (MQ=255)                           tttttCCTAAATCGACGCAACTGTACTCGTCACTACACGTACATACAACGGAGGGGGGCTGCGATTTTTAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcttatttttgttttgggtgtat                                                                                                                       >  2:427882‑M1/1‑120 (MQ=255)                                  tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                                >  2:465478‑M1/1‑113 (MQ=255)                                  tAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                                                                >  2:711149‑M1/1‑113 (MQ=255)                                  tAAATCGACGCAACTGTACTCGTCAATGAAAGATAATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtaatacttttgttttcggtgtatttgaatc                                                                                                                >  2:825646‑M1/1‑113 (MQ=255)                                          cGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaa                                                                                                        <  1:653736‑M1/149‑45 (MQ=255)                                                tGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttt                                                                                                  >  1:533787‑M1/1‑99 (MQ=255)                                                 gTACTCCTCACGACACCTCCATACAACCGAGGGGCGGTCCCATTTTCAATAATGCGTCATTCAGATCACACAAAACCCTCAATTACTTAACATAAATGtataattcatacttttgttttgggtgtatttcaatctgttaaaaagttttc                                                                                                 <  2:254188‑M1/149‑52 (MQ=255)                                                  tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                                <  2:337939‑M1/149‑53 (MQ=255)                                                   aCTCGTCACCACACGCACATACAACGGAGGGTGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                               <  1:235915‑M1/149‑54 (MQ=255)                                                     gcgTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgct                                                                                             >  2:632263‑M1/2‑94 (MQ=255)                                                        tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacg                                                                                          >  1:345356‑M1/1‑91 (MQ=255)                                                         cACTACACGAACATAGAAAGGAGGGAGGCTGAGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATGACTTAACATAAATGtataagtcatacttttgttttgggtgtattaaaaacaaaaaaaaagtttttcgctacgc                                                                                         <  2:665697‑M1/149‑60 (MQ=255)                                                               acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACCCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctcgcaa                                                                                   >  1:93685‑M1/1‑84 (MQ=255)                                                                     aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                             >  2:33781‑M1/1‑78 (MQ=255)                                                                      tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                                                                            <  1:613405‑M1/149‑73 (MQ=255)                                                                      tACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAAAAATCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatatgttaaaaagtttttcgcttcgctagcaagcaaaaa                                                                            >  2:465756‑M1/1‑77 (MQ=255)                                                                         aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATTtataagtcatacgtttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                         <  2:290563‑M1/149‑76 (MQ=255)                                                                         aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctatcaagcaaaaatga                                                                         >  2:275739‑M1/1‑74 (MQ=255)                                                                                   ggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaata                                                               >  1:145026‑M1/1‑64 (MQ=255)                                                                                    gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                              >  1:297346‑M1/1‑63 (MQ=255)                                                                                      tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatg                                                            >  2:252795‑M1/1‑61 (MQ=255)                                                                                               cAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggga                                                   >  1:826622‑M1/1‑52 (MQ=255)                                                                                                aataatGCGTGATGCAGATCACACAAAACACTCGATTACTTAACATAAATGtataagtcatacttttgtcatgggtgtatttcgatctgttaaaaaatttttcgctacgctaccaagcaaaagtgaaaaaggaataaacgaaatgggat                                                  >  2:244867‑M1/1‑51 (MQ=255)                                                                                                aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                                  <  2:488511‑M1/149‑99 (MQ=255)                                                                                                     tccgggatgcaGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttacaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                             <  2:467435‑M1/144‑104 (MQ=255)                                                                                                          gATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaca                                        >  1:216041‑M1/1‑41 (MQ=255)                                                                                                             gCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtc                                     <  2:379128‑M1/149‑112 (MQ=255)                                                                                                             gCAGATCACACAAAACACTCAATTACTTAACATAAATAtatgagtcatacttttgttttgggtgtatttcaatctattaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataaacgaaatgggatgttgcgcacagtc                                     >  1:418805‑M1/1‑38 (MQ=255)                                                                                                                    acacaAAACACTCAATTCCTTAACATTACTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttattcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                              <  1:587511‑M1/149‑119 (MQ=255)                                                                                                                    acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtccaaataa                              >  1:147893‑M1/1‑31 (MQ=255)                                                                                                                              cTCAATTACTAAACATAAATCtataagtcatactggtgtttggggtttatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaa                    <  1:404529‑M1/149‑129 (MQ=255)                                                                                                                               tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa                   >  1:516722‑M1/1‑20 (MQ=255)                                                                                                                                   ttATTTAACATAAATGgagaagtcatacttttgtttggggtgtatttcaatctgttaaaaagtgtctcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaatcaa               <  2:35129‑M1/149‑134 (MQ=255)                                                                                                                                       ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc           >  2:240584‑M1/1‑12 (MQ=255)                                                                                                                                          aCATAAATGtatcagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc        <  2:722244‑M1/149‑141 (MQ=255)                                                                                                                                                aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa  >  1:844951‑M1/1‑3 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913/1978357‑1978648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑