Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,876,477 C→A 10.9% intergenic (‑150/‑102) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,876,4770CA10.9% 80.9 / 3.3 38intergenic (‑150/‑102)cysD/iapsulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base C (23/10);  new base A (2/2);  total (26/12)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

CCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTATGTTTTCCGCATTGCGCCACCGTACCGCTGCCCTGGC  >  NC_000913/2876330‑2876616
                                                                                                                                                   |                                                                                                                                           
ccGTTCCTTTGCAATCCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCa                                                                                                                                            >  2:477218/1‑149 (MQ=255)
  gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAAGGAGTTGGCAAAGCTAGTATCAAAAAGTACGTAAGGGAcc                                                                                                                                          >  1:818637/1‑149 (MQ=255)
  gTTCCTTTGCAATACCGCTATTTTCGTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCAcc                                                                                                                                          <  2:91712/149‑1 (MQ=255)
       tttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGACACCGGAtt                                                                                                                                     >  2:739740/1‑149 (MQ=255)
       ttggCAATACCGCTAGTTTGTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGACATTACACATTAGACTGAGTAGTTCCTTAACGGAATAACGATATGGCAAGGCTAATATCAAAAAGTGCTGAAGGCACGGCAtt                                                                                                                                     >  2:123598/4‑149 (MQ=255)
            aaTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                                                                                                                                >  2:92465/1‑149 (MQ=255)
            aaTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                                                                                                                                >  2:318909/1‑149 (MQ=255)
                 cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATACCGATTTCGCAGAGGTAATATCAAAAAGTGCTTAAGGCACCGGAGTTCGGGCGtgt                                                                                                                           >  2:560187/1‑147 (MQ=255)
                 cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                                                                                                                           >  1:811404/1‑149 (MQ=255)
                 cGCTATTTTCTTGCCATCAGATGTTTCGACAATAGGGACCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                                                                                                                           >  2:82110/1‑149 (MQ=255)
                  gcaaTTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTa                                                                                                                          <  1:61344/146‑1 (MQ=255)
                      ttttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGaa                                                                                                                      >  1:35090/1‑149 (MQ=255)
                        ttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGAACCGGATTTCGGGCGTTTAGGAAGa                                                                                                                    >  2:845068/1‑149 (MQ=255)
                        ttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGAACCGGATTTCGGGCGTTTAGGAAGa                                                                                                                    >  2:844629/1‑149 (MQ=255)
                                 taataagTTTCGACTAGAGGTAACATAAAAGAACGAATGAATTTACCAATTAGAATTAGTAGTTCCTTAACGTAATAACTATTTGGCAAATCTAAAATAAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                                                                           <  2:775200/143‑1 (MQ=255)
                                     aTGTTTCGGCTATAGGGGGCGTAAGAGGACGGATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                                                                       <  2:334888/149‑1 (MQ=255)
                                                 taGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAAGGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAGTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGc                                                                                           <  2:382284/149‑1 (MQ=255)
                                                  aGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCa                                                                                          <  1:304686/149‑1 (MQ=255)
                                                            agagAACGAATGATATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACt                                                                                >  2:521644/1‑149 (MQ=255)
                                                                    aaTGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAGTATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGCTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCCTACTATGGCGGt                                                                        >  2:632543/1‑149 (MQ=255)
                                                                        aaaTTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaaa                                                                    >  1:116734/1‑149 (MQ=255)
                                                                           ttACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGAAAATaaaaaaa                                                                 <  2:851154/149‑1 (MQ=255)
                                                                                           gtagtTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAGGGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGGAAAAAAGTTGCCATAGCATTTaa                                                 >  1:841278/1‑149 (MQ=255)
                                                                                            tagtTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGCGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAg                                                >  1:519022/1‑149 (MQ=255)
                                                                                             agtTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGAACCGGATTTCGGGCGTTTAGGAAGATTTGAACTTTTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTTCATGGTATTTAAgg                                               <  1:844629/149‑1 (MQ=255)
                                                                                             agtTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGAACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTGAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAgg                                               <  1:845068/149‑1 (MQ=255)
                                                                                              gtTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAggc                                              >  2:498181/1‑148 (MQ=255)
                                                                                                  cTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGAcaca                                          >  2:342744/1‑149 (MQ=255)
                                                                                                     aaCGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTa                                       >  1:363607/1‑149 (MQ=255)
                                                                                                      aCGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGGAATTAAAGACTCCCTAt                                      >  2:22288/1‑149 (MQ=255)
                                                                                                         gAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTGAAAAACATGGCGAGGTGGTTAAGGACTCACTATgct                                   >  2:560607/1‑147 (MQ=255)
                                                                                                         gAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAACTTGCATGGTATTTAAGGACTCACTATGtt                                   <  2:207878/149‑1 (MQ=255)
                                                                                                              aCGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTATGTTTTCCg                              >  1:693882/1‑149 (MQ=255)
                                                                                                                gATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTATGTTTTCCGCa                            >  1:190948/1‑149 (MQ=255)
                                                                                                                 aTTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTCTTTAAGGACTCACTATGTTTTCAGCAt                           >  2:554410/1‑149 (MQ=255)
                                                                                                                           gcgaaTATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTATGTTTTCCGCATTGCGCCACCg                 <  1:79786/146‑1 (MQ=255)
                                                                                                                                   cAAAAAGTGCTTAAGGCAGAGGGTTGCGGGCGTTTCGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAGGGCCTCACTATGTTTTCAGAATTGCGCCAGCGTACCGcgg         >  2:819612/1‑147 (MQ=255)
                                                                                                                                          tGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGACGGTAAACAAATTTGCATAGTACTTAAGGACTAAATATGTTTGTCGCATTGCGCCACCGTACCGCTGCCCTGgc  <  2:78397/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATTTAAGGACTCACTATGTTTTCCGCATTGCGCCACCGTACCGCTGCCCTGGC  >  NC_000913/2876330‑2876616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑