Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979269	1979278	10–776	24 [0]	[0] 24	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	18 (0.490)	16/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913/1978359‑1978644                                                                                                                                                |                                                                                                                                               aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                           <  2:326010‑M1/149‑6 (MQ=255) aTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                           <  1:653552‑M1/149‑6 (MQ=255)         ttACACTTATCTAAGATTTTTCCTAAATCGACGCACCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTGTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                                                                   <  1:96565‑M1/149‑14 (MQ=255)                   cTAAGATTTTTCCTCAATCGACGCAACTGTCCTCGTCACTACACGCGCACATGTCGGTGGGGCGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                         <  1:132626‑M1/149‑24 (MQ=255)                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                         <  1:566745‑M1/149‑24 (MQ=255)                               cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                             >  1:219180‑M1/1‑114 (MQ=255)                                   aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAGCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                         <  2:559289‑M1/149‑40 (MQ=255)                                         gCAACTGTACTCGTCCCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatgtcaatctgttaaaaa                                                                                                   <  2:384637‑M1/149‑46 (MQ=255)                                                 aCTCGTCACTACACGCACATACAACGGAGGGCGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcg                                                                                           <  1:38234‑M1/149‑54 (MQ=255)                                                     gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatttgttaaaaagtttttcgctcc                                                                                       >  2:522653‑M1/1‑92 (MQ=255)                                                                   aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaa                                                                         >  2:128144‑M1/1‑78 (MQ=255)                                                                            aggtgtgctgcgaTTTTAAATAACGCGTGATGCAGATCACACAAAACACTCACTTACTTAACACAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                <  1:313375‑M1/143‑81 (MQ=255)                                                                                                      gTGATGCAGATCACACAAAACTCTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgca                                      <  2:103698‑M1/149‑107 (MQ=255)                                                                                                         aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacag                                   >  1:83446‑M1/1‑40 (MQ=255)                                                                                                               aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcattctgttaaaaagtttttcgctacgcaagcaagcaaaaatgacgcgggaataatcgaaatgggatgttgcgcaaagtcaaaa                             >  1:430167‑M1/1‑34 (MQ=255)                                                                                                               aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggctgttgcgcacagtaaaaa                             >  2:59283‑M1/1‑34 (MQ=255)                                                                                                                        agcactcAATTACTGAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcacc                    <  2:558501‑M1/147‑125 (MQ=255)                                                                                                                              aaatTACTTAACATAAATGtataagtcatacttttgttttgtgagtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagaaataatcgaaatgggatgttgcgcacagtcaacataactcaccgtaaat              <  2:316835‑M1/148‑131 (MQ=255)                                                                                                                               aaTTACTTAACATAAATGtataagtcatacttttgttttgggagtcacgcaagccgttgaaatgaaattcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggctgttgcgcacagtcaaaataactcaccgtaaata             >  1:681507‑M1/1‑18 (MQ=255)                                                                                                                                  tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc          <  2:219180‑M1/149‑135 (MQ=255)                                                                                                                                     ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatc       >  1:180933‑M1/1‑12 (MQ=255)                                                                                                                                        aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc    >  1:29657‑M1/1‑9 (MQ=255)                                                                                                                                          aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  >  2:87399‑M1/1‑7 (MQ=255)                                                                                                                                          aTAAAGGtataagttagactgttggtgtgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  >  2:684791‑M1/1‑7 (MQ=255)                                                                                                                                                |                                                                                                                                               ATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913/1978359‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑