Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,394,585 T→C 11.5% I154V (ATC→GTC)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,394,5850TC11.5% 71.3 / 3.9 35I154V (ATC→GTC) nuoLNADH:ubiquinone oxidoreductase, membrane subunit L
Reads supporting (aligned to +/- strand):  ref base T (15/16);  new base C (2/2);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-02

CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATA  >  NC_000913/2394442‑2394727
                                                                                                                                               |                                                                                                                                              
caccaTTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAGCGGTGTAATAGAACCCGATcagca                                                                                                                                           <  2:252653/149‑1 (MQ=255)
caccaTTTCGCGGAAGTTCAGGGTACCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCATCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACACGATcagca                                                                                                                                           >  1:516954/1‑149 (MQ=255)
               gTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGACCGGCGTAATAGAACACGATCAGCAGATAGGAACAAAGGc                                                                                                                            >  2:124475/1‑149 (MQ=255)
                     ggTGCCCAGTTCGTTGTAAAGAATGACCAGTGCGAAAGCGAGGAACACGTCACCCACACGGGCCACGAGGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGACAGGCGCCCAGGCCCCCGc                                                                                                                      >  2:29693/1‑149 (MQ=255)
                     ggTACCCAGTGCGTTGTAAAGAATGAACACTGCGAAAGCGAGGAACACGTCACCCACACGGGCCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGAACGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCACgcgc                                                                                                                      >  2:645050/1‑149 (MQ=255)
                           cAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGCGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCCCGCCTTccc                                                                                                                >  2:411420/1‑149 (MQ=255)
                                     tAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTa                                                                                                      >  2:547926/1‑149 (MQ=255)
                                         gAATGAACAGTGCGAAAGCGAGGAACCCGCTATTCGCACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATc                                                                                                  >  2:667311/1‑149 (MQ=255)
                                            tGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATcagc                                                                                               <  1:686091/149‑1 (MQ=255)
                                             gAACAGTGCTAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACTCTTTCAGTGCCGCTGCGCCATTCTTTGGCTCGGTGGAATAGTACCCGTTCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATcagca                                                                                              <  1:517527/149‑1 (MQ=255)
                                              aaCAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATcagcag                                                                                             >  1:640972/1‑149 (MQ=255)
                                                 aGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATcagcagcag                                                                                          >  1:58857/1‑149 (MQ=255)
                                                     cggaaGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCGCTTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCCCAGGCCCCCGCCTTCCAAGCCGAGGTACATCAGCAGCAGGTTg                                                                                      >  2:121629/4‑149 (MQ=255)
                                                      gAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGt                                                                                     >  2:627652/1‑149 (MQ=255)
                                                         aGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCTTTCTACGTATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCgg                                                                                  <  1:503945/149‑1 (MQ=255)
                                                          gcggagAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGc                                                                                 <  1:92038/144‑1 (MQ=255)
                                                                aaCACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCAc                                                                           >  1:622132/1‑149 (MQ=255)
                                                                 acccGTCACCCACACGGGTCACGACGAACGCTTTCACTGCCGCTGCGCCATTCTTCGGCTCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCAcc                                                                          <  2:576852/146‑1 (MQ=255)
                                                                 acacGTCACGCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCAcc                                                                          <  2:234934/149‑1 (MQ=255)
                                                                         cccACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGACCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAaccac                                                                  >  2:193671/1‑149 (MQ=255)
                                                                         cccACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGACCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAaccac                                                                  >  2:193339/1‑149 (MQ=255)
                                                                                gggTCACGACGAACGCCTTCGTTGCCGCCGCGCCATCCTTCGGATCGGTGTAATAGACCCCGATCAGCAGTTAGTATTAGTGGGTCTTGCCCCCGCGTGTCCGGTAGGGCGGCAGCAGGTTGCCGGCAAGCACCAGAACCACCATGCTg                                                           <  2:663230/149‑1 (MQ=255)
                                                                                     acgacgAACGCTTCCATTGCCGCGGCCCCATTCTTCGGATCGGTCTAATAGAACCCGATCAGCAGATAGGCCCACAGGCCGGCGCTGCTCCAGCCGGGGTTCATTCACTTCGTGTTGTCCGCAAGCACGACCACCACCATGCTGGCGAt                                                      <  2:663192/149‑1 (MQ=255)
                                                                                      cgacgaACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATg                                                     <  1:547926/149‑1 (MQ=255)
                                                                                        acgaACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGaa                                                   <  2:447798/149‑1 (MQ=255)
                                                                                        acgaACGCTTACATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGaa                                                   <  2:396386/149‑1 (MQ=255)
                                                                                                         cgcgtcgcCATCCGTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCga                                  <  2:408828/144‑1 (MQ=255)
                                                                                                             gcgcCATTCTTCGGATCGGTGTAATAGAACCCGACCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCgaagaa                              <  1:193339/149‑1 (MQ=255)
                                                                                                             gcgcCATTCTTCGGATCGGTGTAATAGAACCCGACCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCgaagaa                              <  1:193671/149‑1 (MQ=255)
                                                                                                                    tctccGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGagag                       <  1:432253/149‑1 (MQ=255)
                                                                                                                          gATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACACCAGCAGCAGCTTGTCCGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTGGGCGTAAGCAAAGAAGCCAGCGAAGccc                 >  2:32245/1‑149 (MQ=255)
                                                                                                                                tgtAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGCACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGCAGCCCTCTTCa           >  2:546856/1‑149 (MQ=255)
                                                                                                                                 gtAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCAc          <  2:622132/149‑1 (MQ=255)
                                                                                                                                      aGACCCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCGGCGCAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCgcgc     <  1:569486/149‑1 (MQ=255)
                                                                                                                                         aCCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGAGGAACAGGTTGGTGTAAGCGAAGAAGCGAAAGTAGCCCTCTTCACCGCGCaga  >  2:33853/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CACCATTTCGCGGAAGTTCAGGGTGCCCAGTTCGTTGTAAAGAATGAACAGTGCGAAAGCGAGGAACACGTCACCCACACGGGTCACGACGAACGCTTTCATTGCCGCTGCGCCATTCTTCGGATCGGTGTAATAGAACCCGATCAGCAGATAGGAGCACAGGCCCACGCCTTCCCAGCCGAGGTACATCAGCAGCAGGTTGTCGGCAAGCACCAGAACCACCATGCTGGCGATGAACAGGTTGGTGTAAGCGAAGAAGCGAGAGTAGCCCTCTTCACCGCGCATA  >  NC_000913/2394442‑2394727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑