Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,038 C→A 100% V62L (GTA→TTA)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0380CA100.0% 239.6 / ‑6.7 75V62L (GTA→TTA) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (35/39);  total (35/40)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTG  >  NC_000913/4116898‑4117180
                                                                                                                                            |                                                                                                                                              
acgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTgg                                                                                                                                        <  2:427857/149‑1 (MQ=255)
 cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGa                                                                                                                                       <  1:573638/149‑1 (MQ=255)
  gacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAg                                                                                                                                      <  2:654063/149‑1 (MQ=255)
  gacgGCAATGCCCGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGTAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAg                                                                                                                                      <  1:683217/149‑1 (MQ=255)
     ggCCCTGCCCGCCACTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGAGATTTGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCtt                                                                                                                                   <  2:536145/149‑1 (MQ=255)
       cACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTg                                                                                                                                 <  1:97437/149‑1 (MQ=255)
        aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTgg                                                                                                                                <  1:419139/149‑1 (MQ=255)
         cTGCCAGACAATGGCGTTATAGATTGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTggg                                                                                                                               <  1:90210/149‑1 (MQ=255)
          tGCCAGTCTATGGCGTTTTAGCTTGGCGTGCCGGTTTCGTTTACCCAGACAATAGTGGTTTCAGGCGGGTCCGTAATACCGATAGCTGCAATTTGATCGGATCTGGTATCGGCTTTCGCAAGCACTTCTAACGGCGTGGAGCTTTGGGt                                                                                                                              <  2:326613/149‑1 (MQ=255)
          tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGTTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGt                                                                                                                              >  2:45043/1‑149 (MQ=255)
          tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTgggg                                                                                                                              >  2:253971/1‑148 (MQ=255)
          tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGt                                                                                                                              >  2:45030/1‑149 (MQ=255)
            ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCCCTTCTAACAGCGTGGGGCTTTGGGTgg                                                                                                                            >  2:331498/1‑149 (MQ=255)
            ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGGGCGttggttgg                                                                                                                            >  1:404601/1‑149 (MQ=255)
             cAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGc                                                                                                                           <  2:183721/149‑1 (MQ=255)
                aCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCa                                                                                                                        >  1:619866/1‑149 (MQ=255)
                 cAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAg                                                                                                                       <  2:673718/149‑1 (MQ=255)
                   aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                     <  2:443058/149‑1 (MQ=255)
                   aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGGGGCCCAGAt                                                                                                                     >  2:369782/1‑149 (MQ=255)
                        gTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                                                                                <  2:241927/149‑1 (MQ=255)
                        gTTATAGATAGGCTTGCCGGTATCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                                                                                <  1:480073/149‑1 (MQ=255)
                         ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                                                                               >  1:655404/1‑149 (MQ=255)
                          tataGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                              <  1:46679/149‑1 (MQ=255)
                               atagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                         >  1:687728/1‑149 (MQ=255)
                                 agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCTATAGGGTCgg                                                                                                       >  2:688496/1‑148 (MQ=255)
                                 agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCAAGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt                                                                                                       >  2:630618/1‑149 (MQ=255)
                                   gCTTGCCGTTTCCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                                     <  1:66989/149‑1 (MQ=255)
                                    cTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                                                    <  1:281107/149‑1 (MQ=255)
                                    cTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                                                                    <  2:575271/149‑1 (MQ=255)
                                         cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                               >  1:378566/1‑149 (MQ=255)
                                             ttCTTTTACCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                           <  1:466056/149‑1 (MQ=255)
                                                tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGGTTTCCATCGGGTCGTGTTCCACCTCACCTg                                                                                        >  2:72548/1‑149 (MQ=255)
                                                 ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                       >  1:399276/1‑149 (MQ=255)
                                                     cccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                   <  1:689971/149‑1 (MQ=255)
                                                       cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCTGGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGATTTgg                                                                                 >  1:137350/1‑149 (MQ=255)
                                                       cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCAGGTTTTga                                                                                 >  2:480732/1‑148 (MQ=255)
                                                              ttgggttttCACGCTGGTTCGTAAGACCGATAGCTGCGATTTGATCTGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGTGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                          <  1:77455/143‑1 (MQ=255)
                                                                 tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGc                                                                       <  1:250560/149‑1 (MQ=255)
                                                                   gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                     >  2:27929/1‑149 (MQ=255)
                                                                    tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                    <  2:175480/149‑1 (MQ=255)
                                                                        aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                >  1:652460/1‑149 (MQ=255)
                                                                               ttCGTAATCGCTATAGCTGCGAGTTTATCGGCACTGATATCGGGTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGCTGGCCCAGATTCCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                                                         <  2:648182/149‑1 (MQ=255)
                                                                                  gTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                      >  1:523935/1‑149 (MQ=255)
                                                                                  gTAATACCGATAGCTGCAATTTGATCGCAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                      <  1:187920/149‑1 (MQ=255)
                                                                                   tAATACCGATAGCTGCAATTAGATCGGAACTGATATCGGCTTTCGCCAGCACCTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCCACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                     <  2:554795/149‑1 (MQ=255)
                                                                                      tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                  >  1:2020/1‑149 (MQ=255)
                                                                                      tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                  >  2:575826/1‑149 (MQ=255)
                                                                                         cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                               >  1:679497/1‑149 (MQ=255)
                                                                                          gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                              <  1:63126/149‑1 (MQ=255)
                                                                                               cTGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                                         <  2:47336/149‑1 (MQ=255)
                                                                                                tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAGGatat                                        >  2:240898/1‑149 (MQ=255)
                                                                                                 gCAATTTGCTCGGAACTGTTATCGGCTTTCGCCCGCACTTCTAACCGCCTGGCTTTTTGGGTGTCCCTGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATAtt                                       <  1:331648/149‑1 (MQ=255)
                                                                                                 gCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTCCGCCACGCTAATGATAtt                                       >  1:556675/1‑149 (MQ=255)
                                                                                                  cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGATTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                      >  2:220614/1‑149 (MQ=255)
                                                                                                   aaTTTGATCGGAACTGATATCGGCTTTCGCCAGGACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                     >  1:441866/1‑149 (MQ=255)
                                                                                                      ttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCAt                                  <  2:376078/149‑1 (MQ=255)
                                                                                                       tGATCGGGACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                 <  1:232523/149‑1 (MQ=255)
                                                                                                       tGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGAGCCGCTAAGGATCGTGGCATc                                 >  2:100599/1‑149 (MQ=255)
                                                                                                          tCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTg                              >  1:427531/1‑149 (MQ=255)
                                                                                                          tCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTg                              >  1:427624/1‑149 (MQ=255)
                                                                                                           cGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                             <  1:78551/149‑1 (MQ=255)
                                                                                                              aaCTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATcc                          >  2:402226/1‑149 (MQ=255)
                                                                                                              aaCTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACACTAATGATATTGGCATCGTGATcc                          >  1:113563/1‑149 (MQ=255)
                                                                                                                 tGATATCGGCTTTCGCCAGCACTTCTAACAGCATGGAGCTTTGGGTGGCCCAGATTGCCATTGGGTCGTGTTCTACCCAAACTGGTTTTGGGTAGATTTGCTCAAATTCGAGCTGCGACACGCTAATGATATTGGCGTCGTGATCCAtc                       >  2:101366/1‑148 (MQ=255)
                                                                                                                  gATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTa                      <  2:353688/149‑1 (MQ=255)
                                                                                                                   atatCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                     >  1:36886/1‑149 (MQ=255)
                                                                                                                     atCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATGCATTCCGa                   >  1:208803/1‑149 (MQ=255)
                                                                                                                           tttCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc             <  2:123230/149‑1 (MQ=255)
                                                                                                                                ccAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGCCATCGTGATCCATTACGACCGCGCGGGAg        <  2:523935/149‑1 (MQ=255)
                                                                                                                                 cAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc       >  1:550931/1‑149 (MQ=255)
                                                                                                                                      gttCTAACAGCCTGGCGCTTTGGGTGGCCCAGATTTCCATTGTGTCGTGTTCTACCCAACCTGGTTGTGGGAAGATTTGCTCAAATTCGCGCTGCTACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg  <  1:21967/148‑1 (MQ=255)
                                                                                                                                      cgtCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg  <  1:327080/147‑1 (MQ=255)
                                                                                                                                      cTTCTAACAGCGTGGCGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg  <  1:354967/149‑1 (MQ=255)
                                                                                                                                      cTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg  <  1:630124/149‑1 (MQ=255)
                                                                                                                                      cTTCTAACAGCGTGGAGCGTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAGCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACTCGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg  <  2:496911/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
ACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTG  >  NC_000913/4116898‑4117180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑