| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,153,446 | A→G | 10.5% | intergenic (‑349/+249) | ppc ← / ← argE | phosphoenolpyruvate carboxylase/acetylornithine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,153,446 | 0 | A | G | 10.5% | 106.1 / 3.2 | 53 | intergenic (‑349/+249) | ppc/argE | phosphoenolpyruvate carboxylase/acetylornithine deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (19/26); new base G (5/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTA > NC_000913/4153303‑4153594 | cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg < 1:143220/149‑1 (MQ=255) ttATGGAAATGTTAAAAAATCGCCCCAAGTACCCCCAAAGGTGTAGGTCGGATAAGATGCGCAAGTCTCGCATCCGACATTACTGCTTCATTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTggg < 1:163733/149‑1 (MQ=18) ttATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTggg > 2:579914/1‑149 (MQ=255) ttATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTggg > 2:684740/1‑149 (MQ=255) ttATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTggg > 2:494740/1‑149 (MQ=255) gggAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAt < 2:652414/148‑1 (MQ=255) ggaaTGTTAAAAGCTCGCCCCAAGTAACACCAGAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg < 2:420950/147‑1 (MQ=255) gAACTGTTAAAAAACCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg < 2:265405/149‑1 (MQ=255) gTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa < 2:128414/149‑1 (MQ=255) ttAAAAAATCGCCCCAAGTAACACCTAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAg > 1:347794/1‑149 (MQ=255) ttAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAg > 1:623099/1‑149 (MQ=255) tAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCGTCGTTTGAGt > 1:15323/1‑149 (MQ=255) tAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCAGCCTACAGTTGGGCATCGTTTGAGt > 1:425909/1‑149 (MQ=255) tAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGGATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGt > 1:655646/1‑149 (MQ=255) cAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGTCATCGTTTGAGTCACCGtcggccgg > 1:531578/1‑149 (MQ=255) aaGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGTATCCGACATTATTGCGGCACGGGAGAGTGGCGACAGTGCCGGATGCGGCGCGGGCGCCTTATCCGGCCTACGGTGTGGCATCGTTTGAGTAACGGccggtcggg > 2:659840/1‑148 (MQ=14) tAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATaa > 1:258533/1‑149 (MQ=255) acacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGa < 1:619039/149‑1 (MQ=255) cacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGAt < 1:187099/149‑1 (MQ=12) cacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGAt < 2:530569/149‑1 (MQ=12) cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgcg < 1:643065/149‑1 (MQ=255) gggTGGAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCaa < 1:211313/148‑1 (MQ=255) gtgtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGt > 1:171826/1‑149 (MQ=39) gaGGTCGGATAAGGTGGGCAAGTATCGCAGCCGACACTATTGCGGCACTGGAGTTTGGCAGCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc < 2:679834/148‑1 (MQ=11) aGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCg < 2:596783/149‑1 (MQ=40) aGGCCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCg < 1:246733/149‑1 (MQ=255) aaGATGTGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa < 1:397577/149‑1 (MQ=38) gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGACGTCGGTTGAGTCACTGTCGGTCGAATAAGATGCGCAAGCCTCGCGTCCGAccta > 2:632427/1‑146 (MQ=11) gcgcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCCCTGTCGGTCGGAGAAGATGCGCAAGTATCGCATCCGGCattatt > 1:189825/1‑149 (MQ=255) gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCCGATAAGATCCGCAAGCATCGCGTCCGACATTATTGc > 2:145784/1‑149 (MQ=255) gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGACGGATAAGATGCGCCAGTATCGCATCCGACATTATTGc > 2:113487/1‑149 (MQ=255) gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGACTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGc < 1:494740/149‑1 (MQ=255) aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCACGTATCGCATCCGACattatagagc > 2:323093/1‑144 (MQ=12) gCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa < 1:573326/149‑1 (MQ=38) gACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTgg < 1:391714/149‑1 (MQ=38) gACATTATTGCGGCACAGGAGTTTGGCAACAGTGCCGGATGGGGCGCGGGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCGCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTgg < 2:225155/149‑1 (MQ=255) attattGCCGCACTGGAGTTTCGCAACGGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCaa < 2:35373/149‑1 (MQ=255) tattGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACa < 2:347794/149‑1 (MQ=38) tattGCGGCACAGGAGTTTGGCGACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGCGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACa < 2:567851/149‑1 (MQ=255) cACTGGGGTTTGGCAACAGTGCCGGATGCGGCGCGGGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGa < 2:65471/149‑1 (MQ=255) gAGTTTGGCAACGGTGCAGGATGTGGCGCGGGCTCCTTATCCGGCATACAGGTGGGCATCGTTTGCGTCGCTGTCGGTCGGATAAGATGCGCTAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcgg < 2:434730/149‑1 (MQ=14) tGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAATGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGGGGTTGGCAACAGTGCCGGATGCGGCGCGa > 1:311402/1‑149 (MQ=255) cgCAACAGTGCGGGATGGGGTGGGATGACCATATCTGACCAACACTCGCGCATCGATTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAg < 2:218496/148‑1 (MQ=14) cAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTg > 1:554973/1‑122 (MQ=39) aGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCtt > 1:474602/1‑149 (MQ=255) ccGGATACGGGGGGGGCGCCGTATCCGGCCTACCGGTGGCTATTGTTTGGGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATcc < 2:14607/149‑1 (MQ=11) ccaGCGCCTTATCCGGCCAACAGTTGGCCATCGTTTGAGTCATTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGAGGCGGCGCGAGCGCCTTATCCGGCTTACGGTTg < 1:289201/147‑1 (MQ=14) aGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTggg > 2:341635/1‑149 (MQ=255) cgcCTTATCCGGACTACAGTTGGTCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCa < 1:153891/149‑1 (MQ=255) cTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCGTCg > 2:28874/1‑149 (MQ=255) ccGGCCTACACTTGCCCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGa < 1:186151/149‑1 (MQ=255) cGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAg > 1:18023/1‑149 (MQ=255) cGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGGTTGGCACCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGCTGAGCGGCGTTTGAg > 2:38885/1‑149 (MQ=14) ataGTTGGGCATGGTTTGGGTCGCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCCACAGTGCCGGATGCGGCGCGAGCGTCTTTTCCGGTCTATGGTTGTGCATCGTTTGAGTCACTg < 2:63437/147‑1 (MQ=14) aGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCGCTGTa > 1:535376/1‑149 (MQ=255) | CTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTA > NC_000913/4153303‑4153594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |