Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,793,898 Δ1 bp coding (212/552 nt) btuE ← glutathione peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,793,8970T.99.2% 554.1 / ‑2.6 121coding (213/552 nt)btuEglutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (63/56);  total (65/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 123 total aligned reads displayed.

GCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793749‑1794041
                                                                                                                                                    |                                                                                                                                                 
   cgACTGCGGTCGGCACTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgtt                                                                                                                                                <  1:855140/149‑4 (MQ=255)
     aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCAACTTGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTcc                                                                                                                                             <  1:1922452/149‑1 (MQ=255)
      cTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCa                                                                                                                                            <  2:263287/149‑1 (MQ=255)
       tGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAg                                                                                                                                           >  1:532911/1‑149 (MQ=255)
              ggccgtGCGGCAATCAATTTTGGATACAGCGGATGGCGTCCTTCGCCATTGACTTCAATCTTACTGAACATCGGGAACGGTACCGCGCTTGTGGTGGTACAGTCAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTgg                                                                                                                                    <  2:185234/144‑1 (MQ=255)
              ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTgg                                                                                                                                    >  1:1047626/1‑149 (MQ=255)
              ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTgg                                                                                                                                    <  2:610180/149‑1 (MQ=255)
                cgcTGCGGCAATCAATTTTTGATACAGCGGATGGCTTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGtt                                                                                                                                  <  1:1753010/149‑1 (MQ=255)
                cgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGtt                                                                                                                                  <  1:1665890/149‑1 (MQ=255)
                 gcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTg                                                                                                                                 <  1:457071/149‑1 (MQ=255)
                   tGCGGCAATCAATATTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGCTATAATCTCTTCATAGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCa                                                                                                                               >  1:1391451/1‑149 (MQ=255)
                    gCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCAc                                                                                                                              >  1:1547926/1‑149 (MQ=255)
                     cGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGCAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGACAGTGGTTacacc                                                                                                                             >  2:2033397/1‑148 (MQ=255)
                       gCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCAGGTGGGGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGGAACTGGTTGCACggg                                                                                                                           >  1:1646626/1‑149 (MQ=255)
                          atcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAt                                                                                                                        <  2:302583/149‑1 (MQ=255)
                          atcaatTTTTGATACAGCGGATGGCGTACTTCGCCATTAACTTCAATCTTACTGAACATCGGCAAAGACATCCACCTTATCGTGATGCAGTATGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCCGAAACTGGTTGCACGGGAAt                                                                                                                        >  2:781/1‑149 (MQ=255)
                             aatTTTTGTTATAGCTGATGGCGTCCTACGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGTTTGCACGGGACTccc                                                                                                                     >  2:1203808/1‑149 (MQ=255)
                             aatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATccc                                                                                                                     >  2:1513932/1‑149 (MQ=255)
                              atgtttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCa                                                                                                                    <  2:1812568/146‑1 (MQ=255)
                              atTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCa                                                                                                                    <  1:141266/149‑1 (MQ=255)
                              atTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCa                                                                                                                    <  2:676703/149‑1 (MQ=255)
                               tttttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAg                                                                                                                   <  1:268885/149‑1 (MQ=255)
                                 tttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCa                                                                                                                 >  1:1603719/1‑149 (MQ=255)
                                   tGATACAGCGGATGGCGTCCTCCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCAcc                                                                                                               <  2:1860399/149‑1 (MQ=255)
                                       aCAGCGGATGGCGTCCTTCGCAATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTACAGCAACTGGTTGCACGGGAATCCCAGCCCCCTaa                                                                                                           >  1:1068288/1‑149 (MQ=255)
                                         aGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCACaaaa                                                                                                         >  2:1170825/1‑149 (MQ=255)
                                             gATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTCGCACGGGAATCCCAGCACCATAAAACCtc                                                                                                     <  1:1297641/149‑1 (MQ=255)
                                              aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                                                                                    <  2:267724/149‑1 (MQ=255)
                                              aTGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcg                                                                                                    <  2:1458373/149‑1 (MQ=255)
                                                ggcggcCTTCGCCATTAACTTCAATCTTACTGAACCTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                                                                                                  <  1:1625507/149‑1 (MQ=255)
                                                            cATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCCCCATAAAACCTCGGT‑CGACCCAGGCtt                                                                                     >  2:969550/1‑149 (MQ=255)
                                                             aTTAACCTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCttt                                                                                    <  1:95017/149‑1 (MQ=255)
                                                              ttAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑GGTTCCAGAAACGGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTc                                                                                   >  2:1533128/1‑149 (MQ=255)
                                                               tAACTTCACTCTTACGGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCt                                                                                  <  1:263351/149‑1 (MQ=255)
                                                                aaCTTCATCCTTCTTAAACACCGGGAACGCCACCCCCCTGGTGGTGGTACAGTAAGTTTTAATCTCTTCACCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTGGCACGGGCCCCTGCCGGGGATAAACCCTCGAT‑CGACCCAGGCTTTCTg                                                                                 <  2:445436/149‑1 (MQ=255)
                                                                aaCTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTg                                                                                 >  1:387451/1‑149 (MQ=255)
                                                                   ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAat                                                                              >  2:1427851/1‑149 (MQ=255)
                                                                   ttCAATCTTACTGAACATCGGGAACGTCACCCCCCATGAGGTGGTAAAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCAAGAAACTGGTTGCACGGGCTTCGCATCACCATAAAACTTCGAT‑CGACCCAGGCTTTCTGAat                                                                              >  2:842467/1‑149 (MQ=255)
                                                                         cTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCCGCCCCCTAAAACCTCGAT‑CCACCCAGCCTTACTGAATATtctc                                                                        >  1:382355/1‑149 (MQ=255)
                                                                          ttACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACGTCGAT‑CGACCCAGGCTTTCTGAATATTCTcc                                                                       >  1:1375462/1‑149 (MQ=255)
                                                                            aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCaa                                                                     <  2:799035/149‑1 (MQ=255)
                                                                            aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCaa                                                                     <  2:1547926/149‑1 (MQ=255)
                                                                             cTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAAc                                                                    >  1:126835/1‑149 (MQ=255)
                                                                              tgcaCATTGGGAACGTCACACGCCAAGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTACACGGGAATCCCAGCACCATAAAACCTCGTT‑CGACCCAGGCTTTCTCAATATTCTCCAACt                                                                   >  2:1463771/4‑149 (MQ=255)
                                                                               gAACGTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAATTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTg                                                                  <  2:1741492/149‑1 (MQ=255)
                                                                                aacaacGGGAACGTCACCCCCCCTGTGGTGGTACAGTAAGTTTTAATCTCTTCATCACTGCCCGGTTC‑TGTTACAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGc                                                                 >  2:934024/1‑149 (MQ=255)
                                                                                aaCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGc                                                                 <  1:101185/149‑1 (MQ=255)
                                                                                aaCATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAAT‑CCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCt                                                                >  2:396888/1‑149 (MQ=255)
                                                                                  cATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTc                                                               >  2:1274553/1‑149 (MQ=255)
                                                                                  cATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTc                                                               >  1:667539/1‑149 (MQ=255)
                                                                                   aTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa                                                              >  1:1675268/1‑149 (MQ=255)
                                                                                   aTCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa                                                              >  1:160133/1‑149 (MQ=255)
                                                                                    tCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat                                                             >  1:124772/1‑149 (MQ=255)
                                                                                      gggAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat                                                           >  1:777611/1‑149 (MQ=255)
                                                                                      gggAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCatat                                                           >  2:148705/1‑149 (MQ=255)
                                                                                       ggAACGTCACCCCCCATGTGGCGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAACACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCCCATAtt                                                          >  2:490992/1‑149 (MQ=255)
                                                                                        gAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑GGTTCCAGAAACTGGTTGCACGGGAAACACAAGACGATAAAACCTCGAT‑CGCCCCAGGCTGTCTGAATATTCTCCAAATGCACATATTg                                                         >  1:478327/1‑149 (MQ=255)
                                                                                           cGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcgg                                                      <  2:1602431/149‑1 (MQ=255)
                                                                                            gTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcggc                                                     <  1:1513932/149‑1 (MQ=255)
                                                                                              cACCCCGCATGTGGTGGTACAGTAAGGTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGAGGCGt                                                   >  2:1095875/1‑149 (MQ=255)
                                                                                              cACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCGCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGt                                                   >  1:1318833/1‑149 (MQ=255)
                                                                                                ccccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa                                                 >  1:123606/1‑149 (MQ=255)
                                                                                                 cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                >  1:1905054/1‑149 (MQ=255)
                                                                                                 cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGACCGGTTGTTG‑TCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa                                                >  2:1860386/1‑149 (MQ=255)
                                                                                                  ccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAATCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAg                                               <  2:1332328/149‑1 (MQ=255)
                                                                                                   tccattgggtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCTTTAACCCTCGAT‑CGTCTCAGGCTTTCTGAATATTCTCCATCTGCTCATATTGCGGCGTTAAGc                                              <  2:1345694/142‑1 (MQ=255)
                                                                                                   cccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGc                                              >  2:130728/1‑149 (MQ=255)
                                                                                                     cATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca                                            <  2:708916/149‑1 (MQ=255)
                                                                                                        gtggtgGTACAGTAAGTTTTAATCTCTTACTAGGCG‑GCGTATACTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCacac                                         >  1:496100/1‑149 (MQ=255)
                                                                                                           gtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACttt                                      <  1:1535551/149‑1 (MQ=255)
                                                                                                           gtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAG‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTTCGGCGTTAAGCCACACttt                                      >  1:1066460/1‑149 (MQ=255)
                                                                                                            tggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCGGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTg                                     >  1:95237/1‑149 (MQ=255)
                                                                                                            tggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACCCTTTg                                     >  1:1812197/1‑149 (MQ=255)
                                                                                                            tggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTg                                     >  1:642484/1‑149 (MQ=255)
                                                                                                             ggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGCTGGACACGAAACCCTGCGGCATGAACCAATGCTGTTGTTAATGCTCTGCG‑ATATGCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGa                                    >  2:1956601/1‑149 (MQ=255)
                                                                                                             ggtACAGTAAGTTTTAATCTCTTCAACGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCCTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCAACCTTTGa                                    >  1:1143930/1‑149 (MQ=255)
                                                                                                               tACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTAGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGCATAGTCTCCAACTGCTCCTATTGCGGCGTTAAGCCACACTTTGAgg                                  <  2:1356943/149‑1 (MQ=255)
                                                                                                                aCAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGc                                 >  2:1699849/1‑149 (MQ=255)
                                                                                                                 cAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCg                                >  1:1107302/1‑149 (MQ=255)
                                                                                                                        ttttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGGGGCGTTAAGCCACACTTTGAGGCGACATTgg                         <  2:1499259/149‑2 (MQ=255)
                                                                                                                         tttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGAc                        <  1:1201232/149‑1 (MQ=255)
                                                                                                                          ttAATCTCTTCCTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACa                       <  1:345971/149‑1 (MQ=255)
                                                                                                                          ttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACa                       <  1:181746/149‑1 (MQ=255)
                                                                                                                           tAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTTAGGCGACATTGCCaa                      >  1:235159/1‑149 (MQ=255)
                                                                                                                           tAACCTCTTCCTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACaa                      <  1:311308/149‑1 (MQ=255)
                                                                                                                             aTCTCTTCATCGCTGCCCGGTTC‑TGTCCCAGAAACTGGTTGCACGGGAACCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATc                    <  1:1129329/149‑1 (MQ=255)
                                                                                                                              tctcTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGAAAATCa                   >  2:1727341/1‑149 (MQ=255)
                                                                                                                               ctctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGATAAGCCACACTTTGAGGCGACATTGACAATCaa                  <  1:1308790/149‑1 (MQ=255)
                                                                                                                               ctctCCACCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGTCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCaa                  <  1:603986/149‑1 (MQ=255)
                                                                                                                                tctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAAc                 >  1:2035487/1‑149 (MQ=255)
                                                                                                                                 ctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACCATCAACa                >  2:334043/1‑149 (MQ=255)
                                                                                                                                 ctTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACa                <  1:1713054/149‑1 (MQ=255)
                                                                                                                                  ttCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg               <  1:1427851/149‑1 (MQ=255)
                                                                                                                                    cATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAACCCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGca             <  2:33634/149‑1 (MQ=255)
                                                                                                                                     aTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGcac            <  1:1699849/149‑1 (MQ=255)
                                                                                                                                            cccGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCg     >  2:1273050/1‑149 (MQ=255)
                                                                                                                                             ccGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCCACAGCAAATTACCgg    >  1:903823/1‑149 (MQ=255)
                                                                                                                                              cGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGc   <  1:2025289/149‑1 (MQ=255)
                                                                                                                                              cGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGc   >  2:156530/1‑149 (MQ=255)
                                                                                                                                               ggTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCg  >  1:1740519/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGAT‑CGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGCG  >  NC_000913/1793749‑1794041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑