Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 116 [0] [0] 117 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)110 (0.940) 74/280 0.6 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 117 total aligned reads displayed.

GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258539‑258832
                                                                                                                                                 |                                                                                                                                                    
ttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaaaaggaggtagcaattacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaacgcactaggcccgcataatcgtgaagggaAgtt                                                                                                                                                   >  2:2031065‑M2/146‑148 (MQ=255)
   tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGCaa                                                                                                                                                >  2:1895914‑M2/143‑149 (MQ=255)
    gttaaaaatctgctaatttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                               <  2:399767‑M2/8‑1 (MQ=255)
     ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttacagagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaGGTGCAAAg                                                                                                                                              >  1:132906‑M2/141‑149 (MQ=255)
     ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaaaggatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                              >  1:1596362‑M2/141‑149 (MQ=255)
       aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                            >  1:448553‑M2/139‑149 (MQ=255)
       aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                            >  1:449430‑M2/139‑149 (MQ=255)
         aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAGAGATaa                                                                                                                                          >  1:667742‑M2/137‑149 (MQ=255)
             ttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                      <  1:843327‑M2/17‑1 (MQ=255)
             ttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                      >  1:1384647‑M2/133‑149 (MQ=255)
               gctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                                                                    >  1:202596‑M2/131‑149 (MQ=255)
                 taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                                  >  1:1311631‑M2/129‑149 (MQ=255)
                     ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                              >  1:1041032‑M2/125‑149 (MQ=255)
                     ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                              <  2:1772902‑M2/25‑1 (MQ=255)
                     gtttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                              <  1:497643‑M2/25‑1 (MQ=255)
                      tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgcccttaccgcgtggacacccgtagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                             <  2:1237364‑M2/26‑1 (MQ=255)
                       ttgccaatttggtaaaacagttgcatcacaacaggagaaagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                            <  1:1782124‑M2/27‑1 (MQ=255)
                          ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                         >  1:1563153‑M2/120‑149 (MQ=255)
                          ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggaaAGTGCAAAGATAATCGCTTCTTTTTCGAtt                                                                                                                         >  2:1697766‑M2/120‑149 (MQ=255)
                          ccaatgtggtaaaacagtggcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagtagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                         <  2:315665‑M2/30‑1 (MQ=255)
                           caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                        >  1:623631‑M2/119‑149 (MQ=255)
                           caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                        >  1:2042833‑M2/119‑149 (MQ=255)
                                tggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcctgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                   <  2:1100391‑M2/36‑1 (MQ=255)
                                tggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                   <  1:308777‑M2/36‑1 (MQ=255)
                                tggtaaaacagttgcatcacaacaggagatagcaatgacgtcaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                   <  2:121058‑M2/36‑1 (MQ=255)
                                 ggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                                  <  1:1064513‑M2/37‑1 (MQ=255)
                                   taaaacagttgcatcacaacaggagatagcggtgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATTGATTCTTTTTCGATTGTTTGGCTg                                                                                                                >  2:1651415‑M2/111‑149 (MQ=255)
                                   taaaacagttgcatcacaacaggagatagcaatgacgttaccgggtggacagccgaagagcagattggtcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAAATAATCGATTCTTTTTCGATTGTCTGGCTg                                                                                                                >  1:1025399‑M2/111‑149 (MQ=255)
                                    aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                               <  1:1809223‑M2/40‑1 (MQ=255)
                                    aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                               >  2:1555826‑M2/110‑149 (MQ=255)
                                     aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                              >  2:791886‑M2/109‑149 (MQ=255)
                                       acagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                                                                            >  2:2022274‑M2/107‑149 (MQ=255)
                                        cagttgcatcacaccaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                           <  1:608446‑M2/44‑1 (MQ=255)
                                        cagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGATATGAATATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                           <  1:2020836‑M2/44‑1 (MQ=255)
                                         agttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCg                                                                                                          >  1:1616561‑M2/105‑149 (MQ=255)
                                          gtcgcatcaaaacaggagatagcaatgacgttaccgagtggacacccgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                         <  1:790979‑M2/46‑1 (MQ=255)
                                           ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagcgcagattcatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATCCTTTTTCGATTGTCTGGCTGTATgcgcc                                                                                                        >  1:1596413‑M2/103‑147 (MQ=255)
                                           ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgataaaaaaatttaccgcactaggcccgtatattcgtgaaggtaGGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATgcggc                                                                                                        >  2:1129652‑M2/103‑147 (MQ=255)
                                           ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagaccaatatgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                                                                        >  1:1596556‑M2/103‑149 (MQ=255)
                                             gcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                      <  2:1434664‑M2/49‑1 (MQ=255)
                                              catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcaccaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTGTTTTGATTGTCTGGCTGTATGCGTCAAc                                                                                                     <  1:1529748‑M2/50‑1 (MQ=255)
                                               atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                                                                    >  2:1538943‑M2/99‑149 (MQ=255)
                                               atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                                                                    >  1:790192‑M2/99‑149 (MQ=255)
                                                 cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                                  <  1:276311‑M2/53‑1 (MQ=255)
                                                  acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                 >  1:1619630‑M2/96‑149 (MQ=255)
                                                             tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCg                                                                                      <  1:1937568‑M2/65‑1 (MQ=255)
                                                                 aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                  >  1:709263‑M2/81‑149 (MQ=255)
                                                                      cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  2:463751‑M2/74‑1 (MQ=255)
                                                                            cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                       >  2:798362‑M2/70‑149 (MQ=255)
                                                                            cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                       >  1:671777‑M2/70‑149 (MQ=255)
                                                                            cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtaaattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGaatcc                                                                       >  2:841458‑M2/70‑147 (MQ=255)
                                                                              agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                     <  1:548938‑M2/82‑1 (MQ=255)
                                                                                 ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                  <  1:1773481‑M2/85‑1 (MQ=255)
                                                                                     acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                              <  1:140530‑M2/89‑1 (MQ=255)
                                                                                     acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                              <  1:1536888‑M2/89‑1 (MQ=255)
                                                                                     acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                              <  2:202596‑M2/89‑1 (MQ=255)
                                                                                      gccgaagatgagattgaacaaaaaatttaccgcactacgcccgtatattcgtgaacgtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAGCGGAAGTGCGTGAATTCTGGGGCtggt                                                             <  1:2022360‑M2/90‑1 (MQ=255)
                                                                                      cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCATCGGAAGTGCGTGAATTCTGGGGCtggt                                                             <  2:448553‑M2/90‑1 (MQ=255)
                                                                                      cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                             <  2:449430‑M2/90‑1 (MQ=255)
                                                                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCCACGTGAGACCGGCACCGGAAGTGCGTGAATTCTGGGGCaggtg                                                            >  1:2032509‑M2/59‑149 (MQ=255)
                                                                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAGGTGCGTGAATTCTGGGgctgggg                                                            >  1:520699‑M2/59‑147 (MQ=255)
                                                                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                            >  2:1164636‑M2/59‑149 (MQ=255)
                                                                                       ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                            >  2:336750‑M2/59‑149 (MQ=255)
                                                                                        cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAACGATTCTTTTTCAATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAGGTGCGTGGATTCTGGGGCtggtgg                                                           >  1:635815‑M2/58‑149 (MQ=255)
                                                                                          aagatcagcttgatcaaaaaatttcccgcactaggcccgtatagtcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                         <  2:1025399‑M2/94‑1 (MQ=255)
                                                                                               cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAACGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                                    >  1:873126‑M2/51‑149 (MQ=255)
                                                                                               cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTCTACGAGTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                                    >  1:792945‑M2/51‑149 (MQ=255)
                                                                                                agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                   >  1:1101573‑M2/50‑149 (MQ=255)
                                                                                                agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                   <  1:1841790‑M2/100‑1 (MQ=255)
                                                                                                 gatttaccaaaaaatttaccgcactaggcccgtataatcgtgaaggtaAGTGCAAAGATAATCGATGCTCTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTg                                                  <  1:1083829‑M2/101‑1 (MQ=255)
                                                                                                  atggatcaaaaaatttaccgcgctaggcccgtatattcgtggaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGGATTCTGGGGCTGGTGGCTGGCGCggg                                                 >  2:771574‑M2/48‑149 (MQ=255)
                                                                                                       tcaaaaaatgtaccgcaataggcccgtatattcgtgaaggtaAGTGCTAAGATAATCGATTCTTGTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCCGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                            <  2:667742‑M2/107‑1 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAGCGGAAGTGCGTGAATTCTGGGGCTGGTGGAGGGAGCTGGAAGCGCa                                          >  2:258159‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:1307040‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  2:1867772‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:1690567‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:1780573‑M2/41‑149 (MQ=255)
                                                                                                         aaaaaatttaccgcactaggcccgtatattcgtaaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGGAGCGCa                                          >  2:1142293‑M2/41‑149 (MQ=255)
                                                                                                          aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                         <  1:1441941‑M2/110‑1 (MQ=255)
                                                                                                                 taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATccc                                  <  2:1384647‑M2/117‑1 (MQ=255)
                                                                                                                    cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGGTGGAGCTGGAAGCGCAGGAATCccggt                               >  2:1809693‑M2/30‑147 (MQ=255)
                                                                                                                    cgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                               <  2:1594289‑M2/120‑1 (MQ=255)
                                                                                                                     gcactaggcccgcatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCCATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGttt                              <  2:519481‑M2/121‑1 (MQ=255)
                                                                                                                      cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                             <  2:1383494‑M2/122‑1 (MQ=255)
                                                                                                                        ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                           <  2:1563153‑M2/124‑1 (MQ=255)
                                                                                                                        ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                           <  1:1275788‑M2/124‑1 (MQ=255)
                                                                                                                        ctaggcccgtatagtcgggaaggtaAGTGCAAAGATAATCGATTCCTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                           <  2:2032509‑M2/124‑1 (MQ=255)
                                                                                                                           ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                        <  1:324482‑M2/127‑1 (MQ=255)
                                                                                                                            gcccgtatattctggaaggtaAGTGCAAAGATAATCGATTCTGTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAGCGGCTCCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                       <  2:625760‑M2/128‑1 (MQ=255)
                                                                                                                             cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                      <  2:1619630‑M2/129‑1 (MQ=255)
                                                                                                                                   cattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTTGATGGAGCTGGAAGCCCAGGGATCCCGTTTTACCTACAGTTAcc                <  1:1133938‑M2/135‑1 (MQ=255)
                                                                                                                                     ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAg              >  1:1198661‑M2/13‑149 (MQ=255)
                                                                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCCCCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGTGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt           <  2:744543‑M2/140‑1 (MQ=255)
                                                                                                                                        gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt           <  1:1082097‑M2/140‑1 (MQ=255)
                                                                                                                                         tgaaggtaAGTGTAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg          <  2:1606605‑M2/141‑1 (MQ=255)
                                                                                                                                         tgaaggtaAGTGCAAAGATAATCGATTCTTTTGCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGCTTg          <  2:594441‑M2/141‑1 (MQ=255)
                                                                                                                                          gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg         <  2:671777‑M2/142‑1 (MQ=255)
                                                                                                                                             ggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCt      <  2:1219357‑M2/145‑1 (MQ=255)
                                                                                                                                                aAGTGCAAAGATACTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt   <  2:3057‑M2/148‑1 (MQ=255)
                                                                                                                                                 aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCCGGAAGCGCAAGAATCCCGATTTACCTACAGTTACCAGTTCGGTCTGTTc  >  2:146591/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258539‑258832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: