Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA97.2% 310.4 / NA 108A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (44/59);  minor base C (2/1);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185
                                                                                                                                                |                                                                                                                                                 
gATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACttt                                                                                                                                               >  1:1238284/1‑149 (MQ=255)
 tctttCGCACCCTGGTGGATTTAACCCACGGTAAGGCCATTGCGGTTGCCGATGAAGCCTACATCGAGCTTTGCCCGCAGTCATCGCTGGCTGGCTGGCTGGCGGAATATCCTCACCTGGCTATTTTACGCACACTGTCGAAAACtttt                                                                                                                                              <  2:1411982/147‑1 (MQ=255)
  ctttCGCACCCTGCTGGAGGTAACCAGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCTCAATGTCGAAAACTTTTg                                                                                                                                             <  1:1503964/148‑1 (MQ=255)
       gCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTCCGCACACTGTCGAAAACTTTTGCTCTg                                                                                                                                        >  2:903880/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    >  1:509119/1‑149 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    <  1:786559/149‑1 (MQ=255)
           ccTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgg                                                                                                                                    >  1:786691/1‑149 (MQ=255)
               ctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAGAACTTTTGCTCTGGCGGGGCt                                                                                                                                >  1:613768/1‑149 (MQ=255)
                 ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTc                                                                                                                              >  1:1553425/1‑149 (MQ=255)
                  gAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGACTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCCCACTGCCGAAACCTTTTGCTCAGGCGGGGCTTCg                                                                                                                             >  2:721839/1‑149 (MQ=255)
                    gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                           >  1:2031388/1‑149 (MQ=255)
                      tAACCCGGGGGAAGGCGATTGTGGTTGCCGATGAAGCCTATAGCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  2:248041/149‑1 (MQ=255)
                      tAACCCGCGGTAAGGCGATTGTGGTGGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  1:400000/149‑1 (MQ=255)
                        aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  2:1754628/149‑1 (MQ=255)
                        aCCCGCGGTAAGGCGATGGTGGTTGCCGATGACGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  1:701420/149‑1 (MQ=255)
                        aCCCGCGGGAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTTGCGTTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  2:1754622/149‑1 (MQ=255)
                         cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTGCGTTGCGGa                                                                                                                      >  1:1018524/1‑149 (MQ=255)
                         cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                      >  1:1426614/1‑149 (MQ=255)
                          ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAt                                                                                                                     <  2:393355/149‑1 (MQ=255)
                                  aGGCGATGGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                             <  2:1369037/149‑1 (MQ=255)
                                   ggCGATTGTTGTTGCCGATGAAGCCTATATCGAGGTTTGCCCGCAGGAATTGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTgg                                                                                                            <  1:1681057/149‑1 (MQ=255)
                                   ggCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTgg                                                                                                            <  1:1679212/149‑1 (MQ=255)
                                       aTTGGGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCaaa                                                                                                        <  2:606513/149‑1 (MQ=255)
                                           tgGTTGCCGATTATGCCTATATCGAGTTTGGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTTCGGATTTACGCTGGCAAACgaa                                                                                                    <  2:987497/149‑1 (MQ=255)
                                           tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                    <  2:367559/149‑1 (MQ=255)
                                            ggTTGCCGATGAAGCCTCTATCGGGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                                   <  1:407832/149‑1 (MQ=255)
                                            ggTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaag                                                                                                   >  2:621789/1‑149 (MQ=255)
                                            ggTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTACGTTGCGGATTTACGCTGGCAAACgaag                                                                                                   <  1:1530062/149‑1 (MQ=255)
                                              ttGCCGATGAAGCCTATTTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  1:145756/149‑1 (MQ=255)
                                              ttGCCGAGGCAGCCTACATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  2:847523/149‑1 (MQ=255)
                                                 ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGCTGTACGCTGGCAACCgaagaaagc                                                                                              >  2:1434884/1‑146 (MQ=255)
                                                 ccGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtc                                                                                              <  2:1972129/149‑1 (MQ=255)
                                                  cGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAACCGAAGGAGtca                                                                                             >  1:1893134/1‑149 (MQ=255)
                                                  cGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtca                                                                                             >  1:389664/1‑149 (MQ=255)
                                                  cGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtca                                                                                             >  1:660398/1‑149 (MQ=255)
                                                                tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCGGGATATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCGTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCtgatg                                                                               >  2:116758/1‑149 (MQ=255)
                                                                  gAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTGTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaa                                                                             >  2:677326/1‑149 (MQ=255)
                                                                   tgTTGTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            <  1:314018/148‑1 (MQ=255)
                                                                   aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACGCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            >  1:1748325/1‑149 (MQ=255)
                                                                   aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGGAAACTTTTGCTCTGGCGGGGCTCAGCGCTGGAGTAACGAAGCAGAAAGAGGAAGTCATCAACCTGCTGATGaaa                                                                            >  1:1482011/1‑149 (MQ=255)
                                                                   aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            <  1:647887/149‑1 (MQ=255)
                                                                   aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                            <  1:167483/149‑1 (MQ=255)
                                                                     ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                          >  1:1389489/1‑149 (MQ=255)
                                                                        tGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGAt                                                                       <  2:976333/149‑1 (MQ=255)
                                                                         gCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCCCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATc                                                                      <  1:236078/149‑1 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGGGGAGTTACGCTGGCAAACGAAGAAGTCATCAAACTGCTGATGAAAGTGATAGc                                                                    >  1:167683/1‑149 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGGTGGAAGTGATCGc                                                                    >  2:1536419/1‑149 (MQ=255)
                                                                           ccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGc                                                                    >  2:227112/1‑149 (MQ=255)
                                                                            cGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGGTTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGcc                                                                   >  1:898076/1‑149 (MQ=255)
                                                                             gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                  <  2:786691/149‑1 (MQ=255)
                                                                             gCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                  <  2:560375/149‑1 (MQ=255)
                                                                                 gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTGCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                              >  2:1746775/1‑149 (MQ=255)
                                                                                 acATCGCTGGCTGTCTGGCTGGCGGAATATCCGCACGTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGATGGCAAACGAAGAAGTCATCAACCTGCTGCTGAAAGTGATCGCCCCCtc                                                              >  2:188462/2‑148 (MQ=255)
                                                                                     cGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCg                                                          <  2:1553425/149‑1 (MQ=255)
                                                                                       ctggctggctggctggCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGCTGAAAGTGATCGCCCCCTACCCGct                                                        >  1:562304/1‑149 (MQ=255)
                                                                                       cgggtgggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGct                                                        <  2:509119/143‑1 (MQ=255)
                                                                                        tggctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGccc                                                       >  2:1147719/1‑147 (MQ=255)
                                                                                          gctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCtctc                                                     <  1:2030541/149‑1 (MQ=255)
                                                                                             ggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAGAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGAc                                                  <  2:613768/149‑1 (MQ=255)
                                                                                             ggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGAc                                                  <  2:1933029/149‑1 (MQ=255)
                                                                                              gcgggccggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACg                                                 <  1:1110840/146‑1 (MQ=255)
                                                                                               gtggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                                <  2:1719654/148‑1 (MQ=255)
                                                                                               ctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGc                                                >  1:882635/1‑149 (MQ=255)
                                                                                                tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGcc                                               >  1:741809/1‑149 (MQ=255)
                                                                                                tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCACTCTCGACGcc                                               >  1:1856306/1‑149 (MQ=255)
                                                                                                 ggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                              <  2:259203/149‑1 (MQ=255)
                                                                                                  gctggcGGAATATCCGCACCTGGCTAGTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACTAAGTAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                             <  1:1917766/149‑1 (MQ=255)
                                                                                                  gccgacGGAATCCCCGTACCTCGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGGTGATGAAAGTGATCGCCTCCTACCCGCTCTCGACGCCgg                                             <  1:1603955/144‑1 (MQ=255)
                                                                                                   ctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCCCCCGACGCCGGt                                            >  1:1267744/1‑149 (MQ=255)
                                                                                                     ggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTg                                          <  2:750462/149‑1 (MQ=255)
                                                                                                      gccgcatatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTCGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGc                                         <  2:1373091/144‑1 (MQ=255)
                                                                                                      gcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGGTGc                                         >  1:178610/1‑149 (MQ=255)
                                                                                                        ggAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGACGTCATCCACCTGCTGATGTAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCg                                       >  1:1903388/1‑149 (MQ=255)
                                                                                                         gAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGa                                      <  1:613341/149‑1 (MQ=255)
                                                                                                          aaTATCCGCACCGTGCTATTTTACGCACACTGTCGAAAACTTTTGGTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGAc                                     <  2:1095679/149‑1 (MQ=255)
                                                                                                           atatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACa                                    >  1:1978746/1‑149 (MQ=255)
                                                                                                           atatCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCCGTTGCCGACa                                    >  1:1978540/1‑149 (MQ=255)
                                                                                                           atatCCGCACCTGGCTATTTTACCCCCACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCCACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACa                                    <  2:1346100/149‑1 (MQ=255)
                                                                                                            tttCCGCACCTTGCTATTTTACGCACTCTGTCGAAAACTGTTGCTCTGGCGGGGCTTCGTTGCAGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGAGCGCCCCCTTCCCGCTCTCGACGCCGGTTGCCGACAt                                   <  2:568197/147‑1 (MQ=255)
                                                                                                            tatCCGCTCCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACAt                                   <  2:1581376/149‑1 (MQ=255)
                                                                                                               ccGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGc                                >  2:1945377/1‑149 (MQ=255)
                                                                                                                cGCACCTGGCTATTTTACGCACACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCg                               <  1:1434884/149‑1 (MQ=255)
                                                                                                                  cACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGCCGAAAGTGATCGCCCCCTACCCGCTCTCGCCGCCGGTTGCCGACATTGCGGc                             >  2:897324/1‑149 (MQ=255)
                                                                                                                      tGGCTATTTTACGCACCCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAg                         <  1:250688/149‑1 (MQ=255)
                                                                                                                         cTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCg                      <  1:1746775/149‑1 (MQ=255)
                                                                                                                          tATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                     >  1:338959/1‑149 (MQ=255)
                                                                                                                            ttttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTa                   >  1:934181/1‑149 (MQ=255)
                                                                                                                               tACCCACACGGTCGACAGCTTAGCCACGGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGc                >  2:1976261/1‑149 (MQ=255)
                                                                                                                                tcGCACACGGTCGAAAACTTTGGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGcc               <  2:373740/148‑1 (MQ=255)
                                                                                                                                ccGCACACTGGCGATATCTTTTGCTCTGGCGGGGCTCCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGcc               <  1:1066688/148‑1 (MQ=255)
                                                                                                                                 cGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCGACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGCTAAGccc              >  2:381019/1‑149 (MQ=255)
                                                                                                                                  gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCGGGCAAACGAAGAGGTCATCAACCTGCTGATGAAAGTGATCGCCCCCGACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca             <  2:846391/149‑1 (MQ=255)
                                                                                                                                   cacaCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCcac            <  1:1860450/149‑1 (MQ=255)
                                                                                                                                     cacTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACCAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCACATCCGCTCCCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAg          <  2:1347471/149‑1 (MQ=255)
                                                                                                                                     caatGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAg          <  1:1326686/146‑1 (MQ=255)
                                                                                                                                      acTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGGGGCTCAGGCGTTAAGCCCACAgg         <  2:1389489/149‑1 (MQ=255)
                                                                                                                                           cGAAAACTTTTGCTCTGGCGCGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtc    <  2:711979/149‑1 (MQ=255)
                                                                                                                                            gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGCCAGTGATCGCCCCCTACCCGCTCCCGCCGCCGGTTGCCGACATCGCGGCCCCGGCCTTACGCCCACCGGGACtcg   >  2:1165944/1‑149 (MQ=255)
                                                                                                                                            gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcg   >  1:1018178/1‑149 (MQ=255)
                                                                                                                                             aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  2:431499/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092896‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: