| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,793,897 | Δ1 bp | 100% | coding (213/552 nt) | btuE ← | glutathione peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,793,897 | 0 | T | . | 96.6% | 123.7 / ‑3.3 | 29 | coding (213/552 nt) | btuE | glutathione peroxidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (13/15); minor base A (1/0); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGC > NC_000913/1793750‑1794040 | cgcgACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATTGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtg < 1:211749/149‑2 (MQ=255) tGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAg > 2:387269/1‑149 (MQ=255) ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCCTTAACTTCCATCTTACTGAACATCGGGAACGTCCCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTgg < 1:322843/149‑1 (MQ=255) ggCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTgg < 1:286151/149‑1 (MQ=255) ggCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGATCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACgg < 2:748579/149‑1 (MQ=255) tttGATACAGCGGATGGCGTCCTTCGCCATTCACTTCAATCTTACTGAACATCGGGAACGTCCCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCTAGAAACTGGTTGCACGGGAATCCCAGCa < 1:451507/149‑1 (MQ=255) gTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTT‑ATGTTACAGAAACTGGTTGCACGGGAATCCCAGCACCATCAAACCtcgatcga > 1:150135/1‑149 (MQ=255) tCCTTCGCCATTAACTTCAATCTTACTGAACATAGGGAACGTCACCCCCCAGGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATTAAACCTCGATCGAc > 1:69112/1‑149 (MQ=255) ttCGCGATTAACTTCAATCTTACTGGACATCGGGAACGTCACGAGGAATGAGGGGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCACGCACCATAAAACCTCGCTCGACCCa > 2:377805/1‑149 (MQ=255) tACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCa < 1:284962/149‑1 (MQ=255) tGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCCCCAACt > 1:11153/1‑149 (MQ=255) gTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcggc < 1:716803/149‑1 (MQ=255) ccccccTGTTGTGGTATAGTAAGTTTTAATCTCCTCCTCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCGGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa < 2:428864/149‑1 (MQ=255) cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTaa < 1:570092/149‑1 (MQ=255) ccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGcc > 1:679802/1‑149 (MQ=255) ccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGATCATAACTGTGCGTTAAGcc < 2:651728/149‑1 (MQ=255) cATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAGAAAGCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTTCGGCGTTAAGCca < 1:515617/149‑1 (MQ=255) gtggtgGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCacac < 2:27159/149‑1 (MQ=255) ggtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACCCtt > 1:32154/1‑149 (MQ=255) ggtACAGTAAGTTTTAATCTCTTCATCGCTGCGCGGTTC‑TGTTCCGGGAACTGGTTGCGCGGGAATCCCAGCACCATACAACCTCGCTCGACCCCGGCTTTCTGCATATTCTCCAACTGCCCCTATGGCGGCGTTCAGCCACACTTTgc > 2:617357/1‑148 (MQ=255) gtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAg < 1:387269/149‑1 (MQ=255) aCAGTAAGTTTTAATCTCTTCAGCGCCGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGc < 1:191444/149‑1 (MQ=255) aGTCTTAATCTCTTCATCGCTGCCCGGTTC‑GGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGGCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACAtt < 2:578043/149‑1 (MQ=255) ttttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACACAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGGAACATTGa > 2:264834/1‑149 (MQ=255) ttttAATCTCTTCATCGCTGCCCGGATC‑TGCTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGa > 1:387402/1‑149 (MQ=255) tttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGAc > 1:195917/1‑149 (MQ=255) ttAATCTCTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAACACCTCGATCGACCCAGGCTTTCTGACTATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACa > 1:668164/1‑149 (MQ=255) tctcTTCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCa > 1:384723/1‑149 (MQ=255) ttCATCGCTGCCCGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAg < 1:620983/149‑1 (MQ=255) cGGTTC‑TGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACgggg > 2:355354/1‑148 (MQ=255) | CGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGGC > NC_000913/1793750‑1794040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |