| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,102,557 | C→A | 17.5% | P98P (CCG→CCT) | trmI ← | tRNA m(7)G46 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,102,557 | 0 | C | A | 17.5% | 77.9 / 6.0 | 34 | P98P (CCG→CCT) | trmI | tRNA m(7)G46 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (13/15); new base A (3/3); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAAAAAGCGCGGGGAAATCCAGCATATCTTCGCT > NC_000913/3102411‑3102703 | tcccACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGAAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCgg < 1:528862/147‑1 (MQ=255) ggAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAACCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCa < 2:377806/149‑1 (MQ=255) aaaaaGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAAt > 2:672267/1‑149 (MQ=255) aaaaGAGCTGCACCATGCGCAATGAATTGTCAGGTATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATg > 1:378142/1‑149 (MQ=255) gcAACGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCa < 2:704604/149‑1 (MQ=255) aaTGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAgg > 2:355372/1‑149 (MQ=255) aTTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGATTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGAt < 1:360538/149‑1 (MQ=255) tCAGGAATCATTTTATGCTGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCttt < 2:203448/149‑1 (MQ=255) gAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCCGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTCGcc > 1:475744/1‑149 (MQ=255) gAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:475745/1‑149 (MQ=255) gAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGcc > 1:475854/1‑149 (MQ=255) ttttATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAACGCACTTCAATGCCGAGGAAGTCCTGCCCAGGCCGCTCTTCAGCCATTGcc > 2:357993/1‑149 (MQ=255) gcaCTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGAc > 1:207822/1‑149 (MQ=255) acctCACCCGCATCGTGACACATCCCGCGCAGGTTGCTTGAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCTCTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGc < 2:177922/146‑1 (MQ=255) gTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAAc > 1:123562/1‑149 (MQ=255) ggACACATCACGCGCAGGTTGCTTAACCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGACGACGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAAcc < 2:36461/148‑1 (MQ=255) aCGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAg > 1:63050/1‑149 (MQ=255) aCGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCCCAAg > 1:63007/1‑149 (MQ=255) cgcgCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGc < 1:348109/149‑1 (MQ=255) cgcAGGTTGCTTAAACCTTCGTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGt < 1:672267/149‑1 (MQ=255) aGGTTGCTTAAACCTGCTTCATGCGCAGTAGCCAGGCACGCCCCAACGCCCGGTGACTGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCAc < 1:531308/149‑1 (MQ=255) aaaCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGGTCTTTGGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAACCTCAAGCGTCACCGGCCCTTc > 2:268910/1‑149 (MQ=255) aaCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCa < 1:644432/149‑1 (MQ=255) ccctgcgtcATGCGCAAAAGCCAGGCCCGGAGCAACGCCCGGCGAAGGGACGTCAATGGCGCGGAAGCCCTGCTCCGGGCGATCTTTAGCCACTGCCACCAGCGCCGCCCCCATGCCAACACCAATCTCAAGCGTCACCGGCGCTTCAc < 2:641300/142‑1 (MQ=255) aCCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCAc < 2:627043/149‑1 (MQ=255) ccTTCTTCCTGCTCAGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAACCCAATCTCAAGCGTCACCGGCGCTTCACg < 2:475744/149‑1 (MQ=255) ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACg < 2:475745/149‑1 (MQ=255) ccTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCAGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACg < 2:475854/149‑1 (MQ=255) aTGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCaaaaa < 1:355372/149‑1 (MQ=255) gCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGACACCCTGACGGAACCAATCTCAAGCGTCCCCGGCGCTTCACGGCCAAAAAGCGCGGGGaa > 1:328865/1‑149 (MQ=255) gcacgcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAAAAAGCGCGGGGAAATCCa > 1:123144/1‑149 (MQ=255) acgcacCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCACGCCAAAACCAACCTCAAGCGTCACCGGCGCTTCACCGACCAAAAGCGCGGGGACATCCAGc > 2:433773/1‑149 (MQ=255) acCAACGCCCGGGGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGGTCTTTAGCCAGTGCCACCAGCGACGCCCCCATGCCAAAACCACTCACAAGCGTCACGGGCGCTTCACGGCCAAAAAGCGCGGGGAGACCCAGCagat > 2:359294/1‑149 (MQ=255) gCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAAAAAGCGCGGGGAAATCCAGCATATCTTCGc < 2:123562/149‑1 (MQ=255) cccGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAAAAAGCGCGGGGAAATCCAGCATATCTTCGCt < 1:675113/149‑1 (MQ=255) | TGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCTTCTTCATGCGCAGAAGCCAGGCACGCACCAACGCCCGGTGAATGCACTTCAATGCCGAGGAAGTCCTGCTCAGGGCGATCTTTAGCCATTGCCACCAGCGACGCCCCCATGCCAAAACCAATCTCAAGCGTCACCGGCGCTTCACGGCCAAAAAGCGCGGGGAAATCCAGCATATCTTCGCT > NC_000913/3102411‑3102703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |