Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,366,973 C→T 8.0% S17L (TCA→TTA)  yhcF → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,366,9730CT 8.0% 245.1 / 2.3 91S17L (TCA→TTA) yhcFputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (37/44);  new base T (6/2);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGATTG  >  NC_000913/3366828‑3367119
                                                                                                                                                 |                                                                                                                                                   
ccTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAgc                                                                                                                                                  >  1:455774/1‑149 (MQ=255)
   cacaCTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCa                                                                                                                                               >  1:734028/1‑149 (MQ=255)
    acacTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGGTTGCCGGGAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                                                              >  2:411991/1‑149 (MQ=255)
    acacTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                                                              <  1:604800/149‑1 (MQ=255)
    acacTGACAATGGCGCCAGCTGTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                                                              <  2:70811/149‑1 (MQ=255)
      acTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTGAGCGCGAGc                                                                                                                                            >  1:172648/1‑149 (MQ=255)
      acTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                                                            <  1:39208/149‑1 (MQ=255)
                gcgcgaGCTTTTAAGGATGACGGGGAACTAACCGCCATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCGTTTTTGCCATGTCAGCGCAAGCCGCTGATaga                                                                                                                                  <  1:225645/144‑1 (MQ=255)
                gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGGCGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGACaga                                                                                                                                  >  2:584891/1‑149 (MQ=255)
                   ccAGCTTTTGAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCGGGAAGTGCCTTTTTTGGCATGTCAGCGCGAGCCGCTGATAGAGTa                                                                                                                              >  2:692253/1‑149 (MQ=255)
                   ccAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGGAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTa                                                                                                                              >  2:233586/1‑149 (MQ=255)
                    cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTAt                                                                                                                             <  1:607353/149‑1 (MQ=255)
                    aaGCTTATAAGGATGAAGGGGAACTGACCGACAGGACAGATAGTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTAt                                                                                                                             >  2:442530/2‑149 (MQ=255)
                       cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCaa                                                                                                                          <  1:231662/149‑1 (MQ=255)
                        ttttAAGGATGAAGGGGAACTAACCGAAATGATAGAAAGGACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                                                         >  1:554839/1‑149 (MQ=255)
                           tAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGa                                                                                                                      <  2:22058/149‑1 (MQ=255)
                            aaGTATGAAGGTGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGAc                                                                                                                     <  2:167530/149‑1 (MQ=255)
                               gATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGGAGTGCCTTTTTTGCCATGTCAGAGCAAGCCGCTGATAGAGTATCAATTGACGtt                                                                                                                  >  1:296162/1‑149 (MQ=255)
                                 tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGGTaa                                                                                                                >  1:598313/1‑149 (MQ=255)
                                 tGAAGGGGAACGAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAAGTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTaa                                                                                                                <  1:318806/149‑1 (MQ=255)
                                    aGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATAAATTGACGTTAAggg                                                                                                             >  2:71397/1‑148 (MQ=255)
                                            tAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTTTTTTTTTTGCGCAAGCCGCTGATAGAGTATCTATTGACGTTAAGGTggcgctag                                                                                                     >  2:375121/1‑142 (MQ=255)
                                            tAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTgg                                                                                                     >  1:177011/1‑149 (MQ=255)
                                                    aTGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTCTTTTCCGATGTCAGCAAATGTTTGTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                                                             <  1:363473/149‑1 (MQ=255)
                                                      gACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGCAGCTGCAGc                                                                                           >  2:543933/1‑149 (MQ=255)
                                                       aCAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCt                                                                                          >  1:5101/1‑149 (MQ=255)
                                                        cAGATATTACAGGCGATCTGACGGTCCTGGTGGAGGCAATATGAATGATGTAAAATTTCTGATTGCTGGAAGTGCCTTTTTTGCCATGTCAGCGCATGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCtt                                                                                         <  2:486647/149‑1 (MQ=255)
                                                        cAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCtt                                                                                         <  2:721598/149‑1 (MQ=255)
                                                         aGATATTACAGGCAATCTGACGGTCCTGGAGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTTAGGTGACTCTGGAAGCTGCAGCTTg                                                                                        >  1:458433/1‑149 (MQ=255)
                                                           atatTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                                                      <  1:685021/149‑1 (MQ=255)
                                                            tatTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTAc                                                                                     <  1:145102/149‑1 (MQ=255)
                                                              ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                                                                   >  2:621405/1‑149 (MQ=255)
                                                                aCAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCGGCGCAAGCCGCTGATAGGGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCa                                                                                 <  1:592311/149‑1 (MQ=255)
                                                                 cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCaa                                                                                <  1:196064/149‑1 (MQ=255)
                                                                  aGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAAt                                                                               >  2:712803/1‑149 (MQ=255)
                                                                  aGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAAt                                                                               >  2:712857/1‑149 (MQ=255)
                                                                    gCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGGGACCCTGGAAGCTGCAGCTTGTACTCCAATAc                                                                             >  2:94297/1‑149 (MQ=255)
                                                                      aaTCTGACGGTCCTGGTGGAGGCAATATGAACAATGGAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCTAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTa                                                                           <  1:462797/149‑1 (MQ=255)
                                                                      aaTCTGACGGTCCTGGTGGAGGCAAGATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCTAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTa                                                                           <  1:462832/149‑1 (MQ=255)
                                                                       aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATGGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTaa                                                                          <  1:333414/149‑1 (MQ=255)
                                                                       aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGCAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACtca                                                                          >  2:499295/1‑147 (MQ=255)
                                                                        tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                                                         <  2:233776/149‑1 (MQ=255)
                                                                         cTGACGGTCCTGGGGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTACCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGc                                                                        <  2:378656/149‑1 (MQ=255)
                                                                              ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCGAGTTCAGCGCACGCAACTGTTAGAGTATCAATTGACGTTAAAATTGCTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcgg                                                                   >  1:177816/1‑149 (MQ=255)
                                                                              ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcgg                                                                   >  1:453157/1‑149 (MQ=255)
                                                                                  cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTTTTTTTTTTGCGCAAGCAGCTGATAGAGTAGCAATTGACGTTAAGGTGAGGCTGGAAGCTGCAGCTTGTACTCCACTACTAAGCAACGGCGGa                                                               >  2:703688/1‑149 (MQ=255)
                                                                                  cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                                                               <  1:157638/149‑1 (MQ=255)
                                                                                  cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTATTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAGGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGa                                                               >  2:412602/1‑149 (MQ=255)
                                                                                         aGGCAAAATGAAGAATGTAAAATTACTGATGGCCGGAAGTGCCTTTTTGGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTa                                                        <  2:542299/149‑1 (MQ=255)
                                                                                         aGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTa                                                        <  2:624364/149‑1 (MQ=255)
                                                                                          ggCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTaa                                                       >  2:43653/1‑149 (MQ=255)
                                                                                           gCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGGTAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAACAACGGCGGAGTTGGTAAc                                                      >  2:710918/1‑149 (MQ=255)
                                                                                            cAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACt                                                     <  1:707536/149‑1 (MQ=255)
                                                                                             aaaaTGAACAATGTAAAATTACTGATTGGCGGAAGTGCCTTTTGTGCCAGGTCAGCGCAAGGCGCTGATAGAGGATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt                                                    <  2:66895/149‑1 (MQ=255)
                                                                                             aaaaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTTTTTTTTTTTCTCAAGACGATGATAGAGGATCAAATGACGGGAAGGTGACTGTGGAAGCTGCAGCTTGTACACCAATACAAAGAAACGGAGGAGTTTTTATCtt                                                    >  2:472407/1‑149 (MQ=255)
                                                                                             aaaaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTTTTTTTTTTTCACAAGCCGCAGATAGAGTATCAATTAACGTTGAGGTGGAAGTTGCAGCTGTAGCTTTTACTCCCATACTAAGAAAAGGATGAGATTTTGACtt                                                    >  2:37093/1‑149 (MQ=255)
                                                                                               aaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCg                                                  <  1:149988/149‑1 (MQ=255)
                                                                                               aaTGAACAATGTAAAATTACTGATTACCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCg                                                  <  2:749274/149‑1 (MQ=255)
                                                                                                aTGAACACTGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCgg                                                 <  1:262255/149‑1 (MQ=255)
                                                                                                   aaCAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTTTTTTTTTTTTTCAAGCCGATGAAAGAATATCATTTTACGTGAAGGTGAGTCTGGAATCTGCAGCTTGTTCTCCAATTCTAACCGACGGCGAACTTATTAACTTCgggcg                                              >  2:392702/1‑146 (MQ=255)
                                                                                                   aaCAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAg                                              >  1:164635/1‑149 (MQ=255)
                                                                                                     cAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAGGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTc                                            >  1:531231/1‑149 (MQ=255)
                                                                                                     cAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGGGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTc                                            >  1:502492/1‑149 (MQ=255)
                                                                                                        tGTAAAATTACTGCCTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt                                         <  1:97577/149‑1 (MQ=255)
                                                                                                        tGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAtt                                         >  2:402454/1‑149 (MQ=255)
                                                                                                        tGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGCAGTTAACTCCGGTAGTCAtt                                         >  2:167940/1‑149 (MQ=255)
                                                                                                         ggaaaaTCACTGATGGCCGGAAGCGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAGAGAGTATCAATTGACGTAGAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCACCGGCGGAGGTGTTAACTTCGGTAGTCCTTc                                        >  2:464253/3‑149 (MQ=255)
                                                                                                         gcaaaaTTACTGAATGCCGGAAGTGCCTTTTTTGCCATGGCAGCGCAAGCCGCTGAGAGAGTCTCAAATAACGTTAATGTCACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc                                        <  2:269934/147‑1 (MQ=255)
                                                                                                         gTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATGGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTATACTTCGGTAGTccatc                                        >  2:506520/1‑145 (MQ=255)
                                                                                                         gTAAAATTACTGATTGCCAAAAGTGCCTTTTTTGCTATGTTTACGCAAGCCGCAGAACGCGCATCAATTGAAGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGACGGAGTGGTTAACATAGGTAGTCATTc                                        >  2:464270/1‑149 (MQ=255)
                                                                                                            aaaTTACTGATGGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGt                                     <  1:312273/149‑1 (MQ=255)
                                                                                                            aaaTGACTGATTGCCGGAAGTGCTGTTGTTGCCATGGCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGt                                     <  2:654702/149‑1 (MQ=255)
                                                                                                               ttACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATCCTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCaa                                  <  1:119234/149‑1 (MQ=255)
                                                                                                                tACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAAt                                 <  1:621405/149‑1 (MQ=255)
                                                                                                                   tGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGTAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTATCGTCGGTAGTCATTCAGTCAATAGa                              <  1:419854/149‑1 (MQ=255)
                                                                                                                     aTTGTCCGAAGTGGCCTTTTTGCCATGGTAGCCCCAGCCGCCGAAAGAGTTACCAATTGACGTTAAGGGGCCTCGGGAAGCGTCACCTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACt                            <  2:58155/149‑1 (MQ=255)
                                                                                                                       tGCCGGAAGTGCCTTTCTTGCCATGTCAGCGCAAGCCGCTGATAGAGGATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACttt                          <  1:511702/149‑1 (MQ=255)
                                                                                                                             aaGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGCATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTTAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACgc                    <  1:433390/149‑1 (MQ=255)
                                                                                                                              aGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACgca                   >  2:365516/1‑149 (MQ=255)
                                                                                                                               gTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACgcag                  <  1:585696/149‑1 (MQ=255)
                                                                                                                                 gCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACgcagca                <  2:285981/149‑1 (MQ=255)
                                                                                                                                 gCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACgcagca                <  2:177011/149‑1 (MQ=255)
                                                                                                                                  ccTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCAc               >  1:747556/1‑149 (MQ=255)
                                                                                                                                   cTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCGGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGCCATTCAGTAAATAGACTTTCAATGCAGCACt              >  2:289048/1‑149 (MQ=255)
                                                                                                                                    ttttttGCCATGTAAGCGCAAACCGCTAATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTCGTCCTACACTCTATAGCCTTTCAACGCAGCACTa             >  2:156865/1‑149 (MQ=255)
                                                                                                                                         tGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTacacac        <  1:91701/149‑1 (MQ=255)
                                                                                                                                           ccATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACCCAg      >  1:641374/1‑149 (MQ=255)
                                                                                                                                             aTGTTAGCGCAAGCCGCTGATAGAGTACCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACCAAGCAACGTCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGAt    <  2:455774/149‑1 (MQ=255)
                                                                                                                                             aTGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGAt    <  1:696315/149‑1 (MQ=255)
                                                                                                                                               gTCAGCGCAAGCCGCTGATAGAGTATCAGTTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGATTg  >  1:491865/1‑149 (MQ=255)
                                                                                                                                               gTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGATTg  <  2:502492/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTCAATAGACTTTCAACGCAGCACTACACACAGATTG  >  NC_000913/3366828‑3367119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: