| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,952,942 | A→G | 57.1% | T154A (ACC→GCC) | ilvE → | branched‑chain amino acid aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,952,942 | 0 | A | G | 57.1% | 60.1 / 98.6 | 105 | T154A (ACC→GCC) | ilvE | branched‑chain amino acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (23/21); new base G (30/29); total (54/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GATGTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGA > NC_000913/3952795‑3953087 | gATGTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac < 1:504132/149‑1 (MQ=255) tGTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCCCCAAACACCa > 2:405208/1‑149 (MQ=255) tGTTGGCATGGGAGTAAACCCGCCAGCGGAATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGTAGCACCAAACACCa > 1:472873/1‑149 (MQ=255) gTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCGTCCTGGAACCGCGCAGCACCAAACACCAt < 1:459457/149‑1 (MQ=255) gTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt < 1:146354/149‑1 (MQ=255) gTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt < 1:217111/149‑1 (MQ=255) ttGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATc > 2:680020/1‑149 (MQ=255) ggCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGTAGGGGATCGATGGGAGGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATccc > 1:325960/1‑149 (MQ=255) ggCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATccc > 1:197355/1‑149 (MQ=255) gCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCg > 1:359177/1‑149 (MQ=255) aaCCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCaaa < 2:6410/149‑1 (MQ=255) cccGCCAGCGGGATACTCAACCGACGTGATGATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGCTGCGATGGTTTCCTCCGGGAACCGCGCAGCACCAACCCCCAGCCCCACGGCGGCAAAAg > 2:519274/1‑149 (MQ=255) ccGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCGGTGGGGAGCGTATATGGGCGCAGAAGCGCTGGAGCAGGGGATCGATACGATGGTTTCCTCGTGGAACCGCGCAGCACCAAAAGCCATCCCCACGACGGCAAGAGc > 2:262336/1‑149 (MQ=255) ccGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGc < 2:297202/149‑1 (MQ=255) ccGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGc < 2:297241/149‑1 (MQ=255) gCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCCCCAAACGCCATCCCGACGGCGGCAAAAGCCg > 2:402037/1‑149 (MQ=255) gCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAACAGCCg > 2:504626/1‑149 (MQ=255) atccggATAATCAACCGTCGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCggtg < 1:137408/145‑1 (MQ=255) agtgggATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAACAGGGGATCTATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg < 2:149092/146‑1 (MQ=255) aGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCATGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg < 2:491218/149‑1 (MQ=255) cGGGATACTCAACAGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCCGCCCCAACCGCCATCCCGACGGCGGCCAAAGCCgggggt > 2:457323/1‑149 (MQ=255) ggATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTac > 2:493671/1‑148 (MQ=255) gATACTCCACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGTTGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAAc < 2:36703/149‑1 (MQ=255) gATACTCAATCTACGTGATTAGCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAATGCCGGTGGTAAc < 2:639933/149‑1 (MQ=255) cTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc < 2:130463/149‑1 (MQ=255) tCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCCGCCCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAccc > 2:16713/1‑148 (MQ=255) tCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTacctacct > 2:171773/1‑149 (MQ=255) tCAACCGACGTGATTATCGCTGCTTTCCCGGGGGGAGCGTATCTGGGCGCAGAAGCGCCGGAGCCGGGGATCGATGCGAGGGCTTCCTCCTGGAACCGCGCAGCCCCAAACACCGTCCGCCCGGCCGCACAAGCCGGCGGTAAccccct > 2:16865/1‑144 (MQ=255) aaCCGACGTGATTATCGCTGCTTTCCCGTGGGGCGCGTATCTGGGCGCAGAAGCGATGGAGCCGGGGAACGATGCGATGGTTTCCTCCTGGACACGCGCAGCACCAAACGCCATACCGAAGGCGGCAGAAGCCGGGGGTAACTCCctct > 2:104291/1‑149 (MQ=255) ccGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct < 2:359177/149‑1 (MQ=255) aCGGGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCATCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc < 2:649141/149‑1 (MQ=255) gTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCCCTCTTCCct > 2:548696/1‑149 (MQ=255) tGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctg < 1:312840/149‑1 (MQ=255) gATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGAACGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc > 2:554552/1‑149 (MQ=255) ttctCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg < 2:78137/146‑1 (MQ=255) ataaatcctTGCCCGTGGGGAGCGTAACGGGACTCATAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggt < 1:544422/142‑1 (MQ=255) tCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg < 1:674873/149‑1 (MQ=255) cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg < 1:496061/149‑1 (MQ=255) ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt > 1:346748/1‑149 (MQ=255) ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGCCGGCAAAAGCCGGTGGCAACTACCCCCCTCCCCTGCCGGTGGGt > 1:9236/1‑149 (MQ=255) tgctTTCCCGGGGGGAGGGGATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAGGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa < 2:176836/149‑1 (MQ=255) gctTTCCCGTGGGGAGCGGATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg < 1:138088/149‑1 (MQ=255) tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGGAGCg > 1:485690/1‑149 (MQ=255) tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa > 1:494345/1‑149 (MQ=255) tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa < 1:680020/149‑1 (MQ=255) ccccTGGGGAGCGTACCTCGGCGTAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg < 2:472454/149‑1 (MQ=255) cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGTTGGTGGGTAGCGAAg < 1:487980/149‑1 (MQ=255) ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc < 2:331073/149‑1 (MQ=255) gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgc < 1:327095/149‑1 (MQ=255) gggAGCGTATCGGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc < 1:27440/149‑1 (MQ=255) ggggCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccg < 2:378894/146‑1 (MQ=255) gAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc > 1:626381/1‑149 (MQ=255) tGGGCGCAGAAGCGCTGGAGCAGGGGATCGAGGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAt < 1:688028/149‑1 (MQ=255) tGGGCCCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAt < 1:542187/149‑1 (MQ=255) cAGAAGCGCTGGGGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGaa < 1:364232/149‑1 (MQ=255) cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCCCCGAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAGCAGGaa > 1:344752/1‑149 (MQ=255) aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTGCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAg > 2:468487/1‑149 (MQ=255) aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAg > 1:352305/1‑149 (MQ=255) aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAg > 1:163597/1‑149 (MQ=255) aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACTAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTa > 2:403189/1‑149 (MQ=255) cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATc > 1:611082/1‑149 (MQ=255) cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATc > 1:148831/1‑149 (MQ=255) ggaGGGGACCGATGCGATGGTTTCCTCCTGGAACCGCGCTGCACCAAACACCATCCCGACGGCGGCAAAAGCCAGTATTATCTATCTCCGGTCCCTGCCGGTAGATATCTAAGCGCGCCGCCTCGGTTATCAGTAAGGTATCGCGCTgg < 2:344752/147‑1 (MQ=255) gggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGAtgt < 1:635088/149‑1 (MQ=255) gggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAACGAAGCGCGCCGCCACGGTTGTCAGGAAGGTATCGCGCTGGAtgt > 1:18247/1‑149 (MQ=255) ggtCGGTGCGGTGGTTTCCTCCTGGAACCGGGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGGTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGa < 1:329793/147‑1 (MQ=255) tCGAGGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAAc < 1:725723/149‑1 (MQ=255) gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACgg < 1:442798/149‑1 (MQ=255) atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGAAGCAATGCAGATCAGGAAGGTATCGCGCTGGATGTGAACGGt < 1:554552/149‑1 (MQ=255) tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGtt < 2:738951/149‑1 (MQ=255) tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGATGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGGGAACGGtt > 2:546865/1‑149 (MQ=255) ggTTTCCTCCTGGAACCGCGCAGCACCAATCACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATAtct > 1:457719/1‑149 (MQ=255) gtatccaccTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATAtctc < 2:428265/146‑1 (MQ=255) gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATAtctc < 1:341436/149‑1 (MQ=255) tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATctct < 2:550409/149‑1 (MQ=255) tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGGGAACGGGTATATctct > 1:43281/1‑149 (MQ=255) tcctccTGGAACCGCGCAGCACCAAACACAACCGCAACGCCCGCAAGAGCCCGTGGTAACGACCTTCCGTCCCTTGTTGTGCGTGTGTAAGCGCTGCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGa < 2:438552/149‑1 (MQ=255) ccctcctAGCACCCCGCAGCACCAACCCCCATCCCGACGGCGGCAAAAGCCCGTAGTAACTCCCCTTCTTCCCTCCGTGTTGCTAGCTAACCGCGCCGCCACGGTTATCACGAAGGTATCGCTCTCGATGTAAACAGTTAAAGctctca < 2:304457/148‑3 (MQ=255) cctcctGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGaa > 1:562904/1‑149 (MQ=255) cctcctGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGaa < 2:742000/149‑1 (MQ=255) cctccgGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGaa < 1:306874/149‑1 (MQ=255) ctcctGCAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAg < 1:454174/149‑1 (MQ=255) tcctGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGCACCTACCTCTCTTCCCTGCTGGTGGGTAGCGACGCGCTGTGCGTCTGGTAGCAGGAAGGTATCGCGCTGGATGTGAACGGGTACATCTCTGAAgg > 1:738876/1‑149 (MQ=255) cctGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGCAGgc > 1:43528/1‑149 (MQ=255) cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGgc < 1:632975/149‑1 (MQ=255) ctGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGGGAACGGTTATATCTCTGAAGgcg > 1:274484/1‑149 (MQ=255) tGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGgcgc > 1:482718/1‑149 (MQ=255) ggAACCGCGGACCACCAAACGCCAGCCCGACGGCGGCAACAGCCGGTGGTAACTTCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCa < 1:104291/149‑1 (MQ=255) gAAACGCGCAGGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGGACGGTTATATCTCTGAAGGCGCAg > 2:381864/1‑149 (MQ=255) aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCCCTCTTCCCTGCTGGTGGGTAGCGAAGAGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAgg < 1:623270/149‑1 (MQ=255) ccGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGGGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCg > 2:380375/1‑149 (MQ=255) gcgcAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGaa > 1:130573/1‑149 (MQ=255) gcagcaCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGaaaa < 1:634001/149‑1 (MQ=255) cagcaCCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAAc > 2:47008/1‑149 (MQ=255) caCCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTg > 2:68245/1‑149 (MQ=255) caCCAAACGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGAGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATAGCTCTGAAGGCGCAGGCGAAAACCTg > 1:463785/1‑149 (MQ=255) caCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCGGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTAGCGCGCTGGTTGTGAACGGTTATATCTCGGAAGGCACAGGCGACAACCTg > 1:38372/1‑149 (MQ=255) aCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGCTATACCTCTGAAGGCGCAGGCGAAAACCTGt < 1:405208/149‑1 (MQ=255) ccAAACACCATCCCGACGGCGGCAAAAGCCGGAGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGtt > 1:442669/1‑149 (MQ=255) aaCGCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGa > 2:422482/1‑149 (MQ=255) | GATGTTGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGA > NC_000913/3952795‑3953087 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |