Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,117,038 | C→A | 100% | V62L (GTA→TTA) | glpK ← | glycerol kinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,117,038 | 0 | C | A | 98.6% | 235.6 / ‑5.7 | 79 | V62L (GTA→TTA) | glpK | glycerol kinase |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (30/47); minor base T (0/2); total (30/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.23e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTG > NC_000913/4116893‑4117183 | gCGGTACGCCGGTCCTGTCAGCCATTGGCGTTATCGCTAGGCTTCCCGGTTTC‑TTTTTCCCAGTCTTTAGTTGTTTCACGCTTTTTCGTAATACCGGCAGCGGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAG‑CACTTCTAACAg < 2:432622/149‑1 (MQ=255) ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCg < 1:696655/149‑1 (MQ=255) ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCg < 1:412666/149‑1 (MQ=255) tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTg < 2:206793/149‑1 (MQ=255) tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAAGACCGATAGCTGCAATTTGGTCGGACCTGATATCGGCTTTTGCCGG‑CACTTCTAACAGCGTg > 1:595612/1‑149 (MQ=255) acgGCACTGCCAGAAAATGGCTTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTCTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGGGGAGc < 1:731261/149‑1 (MQ=255) ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTGCGCCTG‑CACTTCTAACAGCGTGGAGCtt < 1:492409/149‑1 (MQ=255) ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑‑TTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCttt < 2:246658/149‑1 (MQ=255) cACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTCGCCAG‑CACTTCTAACCGCGTGGAGCTTTg < 1:515269/149‑1 (MQ=255) aCTGCCAGACAATGGCTTTATAGATAGGCTTGCCGGTTTC‑TTTTGCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTgg < 1:12941/149‑1 (MQ=255) cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTggg < 2:235015/149‑1 (MQ=255) tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGGGGAGCGTTGGGt > 2:626117/1‑149 (MQ=255) ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTgg < 2:192983/149‑1 (MQ=255) ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTgg > 2:64077/1‑149 (MQ=255) cAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGc < 1:608500/149‑1 (MQ=255) aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGTTCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGc < 2:205506/149‑1 (MQ=255) aGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGcc < 2:610061/149‑1 (MQ=255) gACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTAATCGGAACTGATGTCGGCTTTCGCCAC‑CACTTCTAACCGCGTGGAGCTTCGGGTGcccc > 2:204818/1‑149 (MQ=255) gcAATGGCGTTTTAGATGGGCTTTCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCa < 1:283666/148‑1 (MQ=255) aaTGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTGGCCAG‑CACTTCTAAAAGGGTGGAGCTTTGGGTGGGCCAGa > 2:533656/1‑149 (MQ=255) aTGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGGGGAGCTTTGGGTGGCCCAGAt > 2:152930/1‑149 (MQ=255) aTGGCGTTATAGATAGGCTTGCCGGTTTC‑GTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCCTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAt > 1:530187/1‑149 (MQ=255) ggCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTACGGCTTGTGCGTAATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAttt < 1:706100/149‑1 (MQ=255) gCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTc < 1:497767/149‑1 (MQ=255) cGTTATAGATAGGCTTGCCGGTTGC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACTGCGTGGAGCTTTGGGTGGCCCAGATTTcc < 1:488757/149‑1 (MQ=255) cGTTATAAATAGACTTGCCGGTGTC‑TTGTTACCTGACAGTGTTGTTTTTTCGTTGGGTCATAGTACCTATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTcc > 2:554257/1‑149 (MQ=255) ttATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGATCCTTGGGTGGCCCGGATTTccct > 2:568496/1‑147 (MQ=255) tagatagGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg < 1:693862/149‑1 (MQ=255) ggCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg < 1:315605/149‑1 (MQ=255) tGCCGGTTTC‑TTTTTCCCAGACACTAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAATTGATCTCCGGTTTCGCCAGCCGCTTTTTTGA‑CCTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt < 1:743100/149‑1 (MQ=255) gCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCACAGATGTCCATTGGGTCGTGTTCTa > 2:304468/1‑149 (MQ=255) gTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcccc > 1:497214/1‑148 (MQ=255) ttC‑TTTTTCCCAGGCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc < 2:725575/149‑1 (MQ=255) ttC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc > 1:651310/1‑149 (MQ=255) tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGGACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCACTGGGTCGTGTTCTACCCAACCTg < 2:515874/149‑1 (MQ=255) ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg > 2:475018/1‑149 (MQ=255) tttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt < 2:474904/149‑1 (MQ=255) cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg < 1:671507/149‑1 (MQ=255) cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGATTTCGCCCG‑CACTTCTAACAGCGAGGCGCTTTGGGTGGGCCCGCTTCCCATTGGGTCGTGGTCTACCCAACCCGGTTCTgg > 2:70190/1‑149 (MQ=255) aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg < 1:439061/149‑1 (MQ=255) aTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt < 2:581277/149‑1 (MQ=255) ttgTGGTTTCACGCTGGTCCGTAAGACCGGTGGCTGCAACTTGATCGTGACGGATACCGGCTTTCGCCAG‑CACTCCTTACAGCCTGGAGCTTTGGGTGGCCCAGATTTCCATTGCGGTGTGTTCTACCCAACCTGGTTTTGGGTAGAtt < 2:234579/147‑1 (MQ=255) tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt > 1:215435/1‑149 (MQ=255) aGTGGTTTCACGCTGGTTCGGAATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCCTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt < 2:744968/149‑1 (MQ=255) gTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTATCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg < 2:9649/149‑1 (MQ=255) gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg < 2:725159/149‑1 (MQ=255) gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTCCTACCCAACCTGGTTTTGGGTAGATTTg > 1:85083/1‑149 (MQ=255) gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc > 2:740287/1‑149 (MQ=255) gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc < 1:497927/149‑1 (MQ=255) tCACGCTGGTTCGTGATACCGATAGCTCCAATTCGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaaa < 1:611068/149‑1 (MQ=255) aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt > 1:649279/1‑149 (MQ=255) tgttgcGTAATGTCGATAGCTGCAATTTGATCGGAACTGAGATCGGCTTTCGCCCG‑CACTGGTAACAGCGGTGAGCTTTTCGTTGCCCAGATTTCCATGGTGTCGCGTTCTACCCTTCCTGGTTTTGTGTAGATTTGCGCAATTTcgcg < 2:225974/144‑1 (MQ=255) tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg < 2:198483/149‑1 (MQ=255) gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTTTACGCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt > 1:511984/1‑149 (MQ=255) tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc > 2:452727/1‑149 (MQ=255) tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc < 1:595707/149‑1 (MQ=255) aTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTCTTGGGCAGATTTGCTCTAATTCACGCTGCGacac > 2:311465/1‑149 (MQ=255) tACCGATAGCTGCAATCTGATCGGAACTGACATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg < 1:124740/149‑1 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc > 2:740169/1‑149 (MQ=255) gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa < 2:215435/149‑1 (MQ=255) gtggcggCAATTTGAGCGGAGCGGCTAGCGGTTTTCGCCAG‑CACTTCTGACCGCGTGGGGCTTCGGGTGGCCCAGATTCCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt < 2:76087/143‑1 (MQ=255) cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata > 2:656307/1‑149 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTGCCGCTCCCCCCCACTAATGctat > 1:309488/1‑149 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat > 1:119606/1‑149 (MQ=255) tGCAATTTGATCGGAACTGATATCGCCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGACCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGGTAATGatat > 2:72984/1‑149 (MQ=255) gCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGATCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTACTggtctt > 1:428656/1‑144 (MQ=255) cAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg < 2:318050/149‑1 (MQ=255) aaTTTGATCGGAACTGATCGCGGGTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGGGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg < 2:304823/149‑1 (MQ=255) tCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGAGATTGGCATCGTg < 2:298257/149‑1 (MQ=255) tGATATCGGCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCGTCGTGATCCAtt > 1:527398/1‑149 (MQ=255) gCTTTCGCCAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgc < 1:466770/149‑1 (MQ=255) ccAG‑CACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAg < 1:64077/149‑1 (MQ=255) aG‑CACTTCTAATAGCGTGGAGCTTTGTGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCACCTGGTCTTGGGTAGATTTGCTCAAATTCGCGCTGCAACACGCTAATGATATTTGCATCGTGATCCATTACGACCGCGCGGGAGCt < 2:725780/149‑1 (MQ=255) ccTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt < 2:134102/148‑1 (MQ=255) ctgcTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTGTGGGTAGAGTTGCTCAAATTCGCGCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACGACGGCGCGGAGGCtggtg > 2:367322/4‑149 (MQ=255) cTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTGTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg < 1:740287/149‑1 (MQ=255) ttCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGGGCGGGAGCtggtgg > 1:259308/1‑149 (MQ=255) ttCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgc < 2:551754/149‑2 (MQ=255) cTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGgtggtg < 1:659486/149‑1 (MQ=255) | GCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTC‑TTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAG‑CACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTG > NC_000913/4116893‑4117183 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |