Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,117,038 C→A 100% V62L (GTA→TTA)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0380CA98.6% 235.6 / ‑5.7 79V62L (GTA→TTA) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (30/47);  minor base T (0/2);  total (30/49)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTG  >  NC_000913/4116893‑4117183
                                                                                                                                                   |                                                                                                                                                 
gCGGTACGCCGGTCCTGTCAGCCATTGGCGTTATCGCTAGGCTTCCCGGTTTCTTTTTCCCAGTCTTTAGTTGTTTCACGCTTTTTCGTAATACCGGCAGCGGCAATTTGATCGGAACTGATTTCGGCTTTCGCCAGCACTTCTAACAg                                                                                                                                                <  2:432622/149‑1 (MQ=255)
  ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCg                                                                                                                                              <  1:696655/149‑1 (MQ=255)
  ggTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCg                                                                                                                                              <  1:412666/149‑1 (MQ=255)
    tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTg                                                                                                                                            <  2:206793/149‑1 (MQ=255)
    tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAAGACCGATAGCTGCAATTTGGTCGGACCTGATATCGGCTTTTGCCGGCACTTCTAACAGCGTg                                                                                                                                            >  1:595612/1‑149 (MQ=255)
        acgGCACTGCCAGAAAATGGCTTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTCTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGGGGAGc                                                                                                                                        <  1:731261/149‑1 (MQ=255)
          ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTGCGCCTGCACTTCTAACAGCGTGGAGCtt                                                                                                                                      <  1:492409/149‑1 (MQ=255)
          ggCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCttt                                                                                                                                     <  2:246658/149‑1 (MQ=255)
            cACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGCTATCGGCTTTCGCCAGCACTTCTAACCGCGTGGAGCTTTg                                                                                                                                    <  1:515269/149‑1 (MQ=255)
             aCTGCCAGACAATGGCTTTATAGATAGGCTTGCCGGTTTCTTTTGCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTgg                                                                                                                                   <  1:12941/149‑1 (MQ=255)
              cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTggg                                                                                                                                  <  2:235015/149‑1 (MQ=255)
               tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGGGGAGCGTTGGGt                                                                                                                                 >  2:626117/1‑149 (MQ=255)
                 ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTgg                                                                                                                               <  2:192983/149‑1 (MQ=255)
                 ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTgg                                                                                                                               >  2:64077/1‑149 (MQ=255)
                  cAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGc                                                                                                                              <  1:608500/149‑1 (MQ=255)
                   aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGTTCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGc                                                                                                                              <  2:205506/149‑1 (MQ=255)
                   aGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGcc                                                                                                                             <  2:610061/149‑1 (MQ=255)
                    gACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTAATCGGAACTGATGTCGGCTTTCGCCACCACTTCTAACCGCGTGGAGCTTCGGGTGcccc                                                                                                                            >  2:204818/1‑149 (MQ=255)
                     gcAATGGCGTTTTAGATGGGCTTTCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCa                                                                                                                           <  1:283666/148‑1 (MQ=255)
                       aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTGGCCAGCACTTCTAAAAGGGTGGAGCTTTGGGTGGGCCAGa                                                                                                                         >  2:533656/1‑149 (MQ=255)
                        aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGGGGAGCTTTGGGTGGCCCAGAt                                                                                                                        >  2:152930/1‑149 (MQ=255)
                        aTGGCGTTATAGATAGGCTTGCCGGTTTCGTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCCTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                        >  1:530187/1‑149 (MQ=255)
                          ggCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTACGGCTTGTGCGTAATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGAttt                                                                                                                      <  1:706100/149‑1 (MQ=255)
                           gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTc                                                                                                                     <  1:497767/149‑1 (MQ=255)
                            cGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACTGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                                                    <  1:488757/149‑1 (MQ=255)
                            cGTTATAAATAGACTTGCCGGTGTCTTGTTACCTGACAGTGTTGTTTTTTCGTTGGGTCATAGTACCTATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTcc                                                                                                                    >  2:554257/1‑149 (MQ=255)
                              ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGATCCTTGGGTGGCCCGGATTTccct                                                                                                                  >  2:568496/1‑147 (MQ=255)
                                 tagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTgg                                                                                                               <  1:693862/149‑1 (MQ=255)
                                       ggCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtg                                                                                                         <  1:315605/149‑1 (MQ=255)
                                           tGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAATTGATCTCCGGTTTCGCCAGCCGCTTTTTTGACCTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                     <  1:743100/149‑1 (MQ=255)
                                            gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCACAGATGTCCATTGGGTCGTGTTCTa                                                                                                    >  2:304468/1‑149 (MQ=255)
                                                gTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcccc                                                                                                >  1:497214/1‑148 (MQ=255)
                                                  ttCTTTTTCCCAGGCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                              <  2:725575/149‑1 (MQ=255)
                                                  ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                              >  1:651310/1‑149 (MQ=255)
                                                      tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGGACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCACTGGGTCGTGTTCTACCCAACCTg                                                                                           <  2:515874/149‑1 (MQ=255)
                                                       ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                          >  2:475018/1‑149 (MQ=255)
                                                        tttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGt                                                                                         <  2:474904/149‑1 (MQ=255)
                                                             cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                    <  1:671507/149‑1 (MQ=255)
                                                             cAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGATTTCGCCCGCACTTCTAACAGCGAGGCGCTTTGGGTGGGCCCGCTTCCCATTGGGTCGTGGTCTACCCAACCCGGTTCTgg                                                                                    >  2:70190/1‑149 (MQ=255)
                                                              aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                   <  1:439061/149‑1 (MQ=255)
                                                                   aTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt                                                                              <  2:581277/149‑1 (MQ=255)
                                                                    ttgTGGTTTCACGCTGGTCCGTAAGACCGGTGGCTGCAACTTGATCGTGACGGATACCGGCTTTCGCCAGCACTCCTTACAGCCTGGAGCTTTGGGTGGCCCAGATTTCCATTGCGGTGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                             <  2:234579/147‑1 (MQ=255)
                                                                    tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAtt                                                                             >  1:215435/1‑149 (MQ=255)
                                                                     aGTGGTTTCACGCTGGTTCGGAATCCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCCTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAttt                                                                            <  2:744968/149‑1 (MQ=255)
                                                                      gTGGTTTCCCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTATCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg                                                                           <  2:9649/149‑1 (MQ=255)
                                                                      gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTg                                                                           <  2:725159/149‑1 (MQ=255)
                                                                      gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTCCTACCCAACCTGGTTTTGGGTAGATTTg                                                                           >  1:85083/1‑149 (MQ=255)
                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                        >  2:740287/1‑149 (MQ=255)
                                                                         gTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTc                                                                        <  1:497927/149‑1 (MQ=255)
                                                                            tCACGCTGGTTCGTGATACCGATAGCTCCAATTCGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaaa                                                                     <  1:611068/149‑1 (MQ=255)
                                                                              aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                   >  1:649279/1‑149 (MQ=255)
                                                                                  tgttgcGTAATGTCGATAGCTGCAATTTGATCGGAACTGAGATCGGCTTTCGCCCGCACTGGTAACAGCGGTGAGCTTTTCGTTGCCCAGATTTCCATGGTGTCGCGTTCTACCCTTCCTGGTTTTGTGTAGATTTGCGCAATTTcgcg                                                               <  2:225974/144‑1 (MQ=255)
                                                                                  tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                               <  2:198483/149‑1 (MQ=255)
                                                                                    gTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTTTACGCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt                                                             >  1:511984/1‑149 (MQ=255)
                                                                                      tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                           >  2:452727/1‑149 (MQ=255)
                                                                                      tCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                           <  1:595707/149‑1 (MQ=255)
                                                                                           aTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTCTTGGGCAGATTTGCTCTAATTCACGCTGCGacac                                                      >  2:311465/1‑149 (MQ=255)
                                                                                            tACCGATAGCTGCAATCTGATCGGAACTGACATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                     <  1:124740/149‑1 (MQ=255)
                                                                                             aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                    >  2:740169/1‑149 (MQ=255)
                                                                                                gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                 <  2:215435/149‑1 (MQ=255)
                                                                                                 gtggcggCAATTTGAGCGGAGCGGCTAGCGGTTTTCGCCAGCACTTCTGACCGCGTGGGGCTTCGGGTGGCCCAGATTCCCATTGGGTCGGGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                <  2:76087/143‑1 (MQ=255)
                                                                                                     cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGata                                            >  2:656307/1‑149 (MQ=255)
                                                                                                      tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTGCCGCTCCCCCCCACTAATGctat                                           >  1:309488/1‑149 (MQ=255)
                                                                                                      tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                           >  1:119606/1‑149 (MQ=255)
                                                                                                      tGCAATTTGATCGGAACTGATATCGCCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGACCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGGTAATGatat                                           >  2:72984/1‑149 (MQ=255)
                                                                                                       gCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGATCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTACTggtctt                                          >  1:428656/1‑144 (MQ=255)
                                                                                                        cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                         <  2:318050/149‑1 (MQ=255)
                                                                                                         aaTTTGATCGGAACTGATCGCGGGTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGGGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                        <  2:304823/149‑1 (MQ=255)
                                                                                                                tCGGAACTGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGAGATTGGCATCGTg                                 <  2:298257/149‑1 (MQ=255)
                                                                                                                       tGATATCGGCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCGTCGTGATCCAtt                          >  1:527398/1‑149 (MQ=255)
                                                                                                                               gCTTTCGCCAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgc                  <  1:466770/149‑1 (MQ=255)
                                                                                                                                      ccAGCACTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAg           <  1:64077/149‑1 (MQ=255)
                                                                                                                                        aGCACTTCTAATAGCGTGGAGCTTTGTGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCACCTGGTCTTGGGTAGATTTGCTCAAATTCGCGCTGCAACACGCTAATGATATTTGCATCGTGATCCATTACGACCGCGCGGGAGCt         <  2:725780/149‑1 (MQ=255)
                                                                                                                                            ccTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt      <  2:134102/148‑1 (MQ=255)
                                                                                                                                             ctgcTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTGTGGGTAGAGTTGCTCAAATTCGCGCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACGACGGCGCGGAGGCtggtg     >  2:367322/4‑149 (MQ=255)
                                                                                                                                             cTTCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACCCTGGTTGTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg     <  1:740287/149‑1 (MQ=255)
                                                                                                                                              ttCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGGGCGGGAGCtggtgg    >  1:259308/1‑149 (MQ=255)
                                                                                                                                              ttCTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgc    <  2:551754/149‑2 (MQ=255)
                                                                                                                                                cTAACAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGgtggtg  <  1:659486/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTG  >  NC_000913/4116893‑4117183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: