Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,313,472 C→T 5.7% T41I (ACT→ATT)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,313,4720CT 5.7% 197.0 / 2.8 71T41I (ACT→ATT) rpiBribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (34/32);  new base T (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

AGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCG  >  NC_000913/4313326‑4313615
                                                                                                                                                  |                                                                                                                                               
aGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTg                                                                                                                                               >  1:41572/1‑149 (MQ=255)
  ttttGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGGGCGTAATGAt                                                                                                                                             >  2:324855/1‑149 (MQ=255)
  ttttGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGAt                                                                                                                                             >  2:90662/1‑149 (MQ=255)
     tGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTAt                                                                                                                                          <  1:531047/149‑1 (MQ=255)
      gCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATc                                                                                                                                         <  1:738171/149‑1 (MQ=255)
       cACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATcc                                                                                                                                        >  1:316369/1‑149 (MQ=255)
        acggacggGGAAGATGAATGAATAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGATCCTGGTCGTCAGTGCGTACTGATTATCca                                                                                                                                       <  1:476895/149‑1 (MQ=255)
          ggacggGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCcaca                                                                                                                                     <  1:432586/149‑1 (MQ=255)
             cggGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTACAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACCTTa                                                                                                                                  >  1:516035/1‑149 (MQ=255)
             cggGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTa                                                                                                                                  <  1:317713/149‑1 (MQ=255)
                  aaGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATCTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCa                                                                                                                             <  1:473680/149‑1 (MQ=255)
                        aatgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAg                                                                                                                       <  1:129891/149‑1 (MQ=255)
                         atgaaAAAGATTGCATTTGGCTGTGATCGGGTCTTGATCATTTTAAAACATGAAATAGTGACACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGt                                                                                                                      >  2:536288/1‑149 (MQ=255)
                         atgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGt                                                                                                                      <  1:160625/149‑1 (MQ=255)
                         atgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGt                                                                                                                      <  1:654727/149‑1 (MQ=255)
                         atgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGt                                                                                                                      >  1:536054/1‑149 (MQ=255)
                          tgaaAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTc                                                                                                                     >  1:715913/1‑149 (MQ=255)
                            aaaaaGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGc                                                                                                                   >  1:273467/1‑149 (MQ=255)
                             aaaaGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTATTGATTATCCACATTACGCCAGTCAAGTCGCa                                                                                                                  >  2:750353/1‑149 (MQ=255)
                             aaaaGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTATTGATTATCCACATTACCCCAGTCAAGCCGcc                                                                                                                  >  2:751313/1‑148 (MQ=255)
                             aaaaGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCa                                                                                                                  <  1:195156/149‑1 (MQ=255)
                                 gATTGTATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTgg                                                                                                              <  2:665225/149‑1 (MQ=255)
                                   ttGCATTTGGCTGTTATCATGTTGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCt                                                                                                            <  1:549988/149‑1 (MQ=255)
                                   ctGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCt                                                                                                            <  1:168782/148‑1 (MQ=255)
                                        tttGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGGTAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGc                                                                                                       >  1:446076/1‑149 (MQ=255)
                                         ttGGCTGTGATCATGTTGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGAGGAGCTGAAGTGATTGATAAAGGAACCCGGGCGGCTGGGCGTACTGAGTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTCCt                                                                                                      >  2:531386/1‑149 (MQ=255)
                                                tgATCATGTCGGTTTCATTTTAAACCATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCGCTGGCTGTTGCTGGCGgag                                                                                               >  1:558173/1‑149 (MQ=255)
                                                 gATCATGGCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGgaga                                                                                              <  1:594580/149‑1 (MQ=255)
                                                  aTCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGagag                                                                                             >  1:647260/1‑149 (MQ=255)
                                                     aTGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTATTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGtt                                                                                          <  1:751313/149‑1 (MQ=255)
                                                     aTGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTATTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGtt                                                                                          <  1:750353/149‑1 (MQ=255)
                                                        tCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGAt                                                                                       >  1:488987/1‑149 (MQ=255)
                                                         cGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATg                                                                                      <  2:136969/149‑1 (MQ=255)
                                                          ggTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATgg                                                                                     >  2:747745/1‑149 (MQ=255)
                                                            tttCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCg                                                                                   >  2:266978/1‑149 (MQ=255)
                                                                aTTTTAAAACATGAAATAGTGGCACATGTAGTTGAGCGTGGCGTTGCAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGGCGCACTGGCTGCTGCTGGCGGAGAGGTTTACGGCggggt                                                                               >  2:666061/1‑147 (MQ=255)
                                                                 ttttAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGCtt                                                                              >  2:17257/1‑149 (MQ=255)
                                                                 ttttAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGATGATGGCGGGAtt                                                                              >  2:685694/1‑149 (MQ=255)
                                                                  tttAAAACATGAAATAGTGGAACATTTAGTTGAGCGTGGCGTTGAAGTGACTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGAttt                                                                             <  2:500748/149‑1 (MQ=255)
                                                                             aaaTAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGt                                                                  >  1:690603/1‑149 (MQ=255)
                                                                                   tGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGc                                                            >  1:665104/1‑149 (MQ=255)
                                                                                     gCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCgg                                                          >  2:292796/1‑148 (MQ=255)
                                                                                     gCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGATGTTGATTTGTGGTAGTGGCGt                                                          >  2:254068/1‑149 (MQ=255)
                                                                                          tttAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGATGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTa                                                     >  2:654106/1‑149 (MQ=255)
                                                                                              gTTGAGCGTGGCGTTGAAGTGAGTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTc                                                 <  1:66351/149‑1 (MQ=255)
                                                                                               ttGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCCGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTGGATGGCGGGATGTTGAGTGGTGGTCCTGGCGTCGGCATTTCg                                                >  2:242298/1‑149 (MQ=255)
                                                                                                 gAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTGGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGGGGTTGATGGCGGGATGTTGGTTTGTGGTCCTGGGGTCGGTATTTCGAt                                              >  2:464766/1‑149 (MQ=255)
                                                                                                        gCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAgcggcg                                       <  1:750620/149‑1 (MQ=255)
                                                                                                         cGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGGATTTCGATAGCGGCGa                                      >  2:234831/1‑149 (MQ=255)
                                                                                                          gtggAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGGACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGGTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGaa                                     <  2:145613/146‑1 (MQ=255)
                                                                                                          gTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGaa                                     <  2:273467/149‑1 (MQ=255)
                                                                                                          gTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGGCGGTATTTCGATAgcggcggg                                     >  1:698818/1‑147 (MQ=255)
                                                                                                             gAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACaa                                  <  2:322638/149‑1 (MQ=255)
                                                                                                               aGTGATTGATAAAGGAACCTGGTCGTCAGATCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGt                                <  1:618826/149‑1 (MQ=255)
                                                                                                                 tgattgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGttt                              <  2:202984/149‑1 (MQ=255)
                                                                                                                   attgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGc                            <  1:569601/149‑1 (MQ=255)
                                                                                                                   attgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGCCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGCTGATGGCGGGATTTTGATTCGTGGTACCGGCGTCGGTATTCCGGTAGCGGCGAACAAGTTTGc                            >  2:505009/1‑149 (MQ=255)
                                                                                                                    ttgatAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGcc                           <  2:488987/149‑1 (MQ=255)
                                                                                                                       atAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGGTTATGGTTTGTGGTACTGGGGACGGTGTTTTGAAAGTGCGAACACGtttcacggga                       >  2:641339/1‑142 (MQ=255)
                                                                                                                       atAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGa                        >  2:701349/1‑149 (MQ=255)
                                                                                                                             gAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTcgc                  >  2:596656/1‑149 (MQ=255)
                                                                                                                                    gtcgtcAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTAATAGCGGAGAGGTGGATGGCGGGGTTTTGATTTGTGGTACTGGCGTCGGAATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGAGCGgtcg           >  1:178924/1‑149 (MQ=255)
                                                                                                                                     tcgtcAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGgtcgt          <  2:606281/149‑1 (MQ=255)
                                                                                                                                      cgtcAGAGCGGACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGgtcgtc         <  1:257493/149‑1 (MQ=255)
                                                                                                                                      cgacAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGACGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATGCGCGCGgtcgtc         >  2:297653/4‑149 (MQ=255)
                                                                                                                                        tcAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTg       >  2:430368/1‑149 (MQ=255)
                                                                                                                                          agagCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGCTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTa     <  1:556038/149‑1 (MQ=255)
                                                                                                                                           gagCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAg    <  1:596184/149‑1 (MQ=255)
                                                                                                                                           gagCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAg    <  1:595977/149‑1 (MQ=255)
                                                                                                                                             gCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCg  <  1:623159/149‑1 (MQ=255)
                                                                                                                                             gCGTACTGATTATCCACAGTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCg  <  1:464766/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCAAGTCGCACTGGCTGTTGCTGGCGGAGAGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCG  >  NC_000913/4313326‑4313615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: