Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,793,898 Δ1 bp coding (212/552 nt) btuE ← glutathione peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,793,8970T.94.6% 146.3 / 2.6 38coding (213/552 nt)btuEglutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (20/15);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGG  >  NC_000913/1793751‑1794039
                                                                                                                                                  |                                                                                                                                              
gcgACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCGATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCtgt                                                                                                                                              >  1:900843/1‑147 (MQ=255)
     tGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAg                                                                                                                                        >  2:924719/1‑149 (MQ=255)
      gCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTGATCTCTTCATCGCTGCCCGGTTCTGTTCCAGa                                                                                                                                       >  1:642928/1‑149 (MQ=255)
         gTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGGCCAGTAAGTTTTAATCTCTTCATCCGTGCCCGGTTCTTTTCCAGAAAc                                                                                                                                    >  2:257868/1‑149 (MQ=255)
          tCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCCGAAACt                                                                                                                                   >  2:796856/1‑149 (MQ=255)
                    ggCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACgg                                                                                                                         <  1:212956/149‑1 (MQ=255)
                       aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTCAGTTGAATGTTGCTGAACATCGGGAACGTCACCCCCCATGTGGTTGTACAGTTAGTTTTAATCTCTTCATAGCCCCCCGGGTATGTTCCCGCACCGGGTTGCACGGGaa                                                                                                                      >  1:373389/1‑149 (MQ=255)
                       aatcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGGGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGTTTGCACGGGaa                                                                                                                      >  1:130197/1‑149 (MQ=255)
                       aatcaatTTTTGATACAGCGGATAGCGTCCTTCGCCAGTAACTTCAATCTTGCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGCGaa                                                                                                                      >  1:510949/1‑149 (MQ=255)
                         tcaatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATc                                                                                                                    <  2:556528/149‑1 (MQ=255)
                           aatTGTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATccc                                                                                                                  <  1:283231/149‑1 (MQ=255)
                                   aTACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                                                                                          <  1:497129/149‑1 (MQ=255)
                                   aTACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                                                                                          >  1:501817/1‑149 (MQ=255)
                                    tACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATa                                                                                                         >  1:348989/1‑149 (MQ=255)
                                    tACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATa                                                                                                         <  1:373297/149‑1 (MQ=255)
                                       aGCGGAGGGCCTCCTTCGCCATTAACTTCAATCGTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa                                                                                                      <  2:91775/149‑1 (MQ=255)
                                          ggACGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCt                                                                                                   <  1:234613/149‑1 (MQ=255)
                                                     ttCGCCATTAACTTCAATCTTACTGAAAATCGGGAACGTCACCCGCCATGTGGTGGTACAGTAAGTGTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCCGAAACTGGTTGCACGGGACTCCCAGCACCCTAAAGCCGCGATCAAccac                                                                                        >  2:237587/1‑147 (MQ=255)
                                                           aTTAACTTCAAGTTTGCTGAACTTCGGGAACGTCACCCCCCCTGTGGTGGGCCAGTAATTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCttt                                                                                  <  2:900875/149‑1 (MQ=255)
                                                               aCTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTGAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCCTTCTGa                                                                              >  1:351066/1‑149 (MQ=255)
                                                                cTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGGCCCAGGCTTTCTGaa                                                                             >  1:70653/1‑149 (MQ=255)
                                                                     aTCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc                                                                        <  2:485377/149‑1 (MQ=255)
                                                                          aCTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa                                                                   <  1:797770/149‑1 (MQ=255)
                                                                            tGAACACCGGGAACCGCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACt                                                                 <  1:408848/149‑1 (MQ=255)
                                                                                   cGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATACAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCata                                                          >  2:4787/1‑149 (MQ=255)
                                                                                       aaCGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgc                                                      <  1:924719/149‑1 (MQ=255)
                                                                                               cccccATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATAGTTCGGCGTTaa                                              >  1:159991/1‑149 (MQ=255)
                                                                                                         gtggtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACttt                                    >  1:860851/1‑149 (MQ=255)
                                                                                                            gtACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGCTCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAg                                 <  1:459947/149‑1 (MQ=255)
                                                                                                              aCAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCCCACTTTGAGGc                               >  1:807635/1‑149 (MQ=255)
                                                                                                                 gTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCGCAGCACCATAAAACCGCGAACGACCCAGGCTTACTGCATATTCTCGAACTGCCCATATTGCGGCGTTAGGCCACAATTTGAACCGAc                            >  2:255602/1‑149 (MQ=255)
                                                                                                                  tAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAACCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa                           >  2:392674/1‑149 (MQ=255)
                                                                                                                      ttttAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGa                       >  1:827198/1‑149 (MQ=255)
                                                                                                                       tttAATCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCCCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGAc                      >  1:457122/1‑149 (MQ=255)
                                                                                                                       tttAATCTCTGCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTGGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAACCCACACTTTAAGGAGACATTGaa                      >  2:181423/1‑148 (MQ=255)
                                                                                                                           aTCTCTTCATCGCTGCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATc                  <  1:353949/149‑1 (MQ=255)
                                                                                                                                  cctCGCTGCCCGGTTCTTTTCCAGAGACTGGTTGCCCGGGAATCCCAGCACCATAAAACCTCGATCGTCCCGGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGca           <  2:313842/147‑1 (MQ=255)
                                                                                                                                         gCCCGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTAcc    <  2:351066/149‑1 (MQ=255)
                                                                                                                                           ccGGTTCTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTCCCgg  >  1:510655/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATTGACAATCAACAGCACATTACCGG  >  NC_000913/1793751‑1794039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: