Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,093,040 G→A A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA93.6% 141.3 / NA 47A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/23);  minor base C (2/1);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCG  >  NC_000913/2092897‑2093184
                                                                                                                                               |                                                                                                                                                
aTTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtttt                                                                                                                                             <  1:682250/149‑1 (MQ=255)
    tCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAACCTTTTGctc                                                                                                                                         >  1:354307/1‑149 (MQ=255)
    tCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGctc                                                                                                                                         >  1:368840/1‑149 (MQ=255)
       cACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTCTATCGAGTTTTGCCCGCGGGCATAGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTCTTTTACGCACACTGTCGAAAACTTTTGCTCTga                                                                                                                                      >  2:78410/1‑148 (MQ=255)
               tgGAGTTAACCCGCGGTAAGGCGCTTGTGGTTGCCGATGAAGCCCAGTTCGAGTTTTGCCCTCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTCTGCACCTCATACTGTCGAAAACTTTTGCTCTGGCGGGGCtt                                                                                                                              <  1:462467/149‑1 (MQ=255)
                ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtt                                                                                                                                               >  2:259856/1‑131 (MQ=255)
                ggAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACtt                                                                                                                                               <  1:259856/131‑1 (MQ=255)
                ggAGTTAACCCGCGGCAAGGGGATTGTGGTTGCCGGTGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCAAACAGTAGAAAACTTTTGCTCTGGCGGGGCTTc                                                                                                                             <  2:487497/149‑1 (MQ=255)
                    ttAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTg                                                                                                                         >  2:850194/1‑149 (MQ=255)
                     tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                        <  2:486262/149‑1 (MQ=255)
                       aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                      >  2:272312/1‑149 (MQ=255)
                       aCCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCACGGTCCCCGTGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                      <  1:220957/149‑1 (MQ=255)
                        cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                     <  1:581991/149‑1 (MQ=255)
                         ccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAt                                                                                                                    <  1:701572/149‑1 (MQ=255)
                                   gCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACCCTGGc                                                                                                          >  2:106506/1‑149 (MQ=255)
                                        tgtgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGATGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACg                                                                                                     <  1:545863/149‑1 (MQ=255)
                                          tgGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaa                                                                                                   <  2:764283/149‑1 (MQ=255)
                                             ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                <  1:772895/149‑1 (MQ=255)
                                                     gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCaa                                                                                        <  1:373689/149‑1 (MQ=255)
                                                     gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTAGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCaa                                                                                        >  2:770534/1‑149 (MQ=255)
                                                       aGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATGTCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCGTCaaac                                                                                      >  1:837288/1‑147 (MQ=255)
                                                         ccTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCCCTGGCGGCGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctg                                                                                    <  2:157977/149‑1 (MQ=255)
                                                             tatCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtga                                                                                >  1:176898/1‑149 (MQ=255)
                                                               tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatt                                                                              >  1:483680/1‑148 (MQ=255)
                                                               tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatg                                                                              >  2:126601/1‑149 (MQ=255)
                                                               tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatg                                                                              >  1:483683/1‑149 (MQ=255)
                                                                cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatga                                                                             <  1:272312/149‑1 (MQ=255)
                                                                cGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCtgatga                                                                             >  1:625560/1‑149 (MQ=255)
                                                                  aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                           <  1:880641/149‑1 (MQ=255)
                                                                  aGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaaa                                                                           <  2:357188/149‑1 (MQ=255)
                                                                    ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAAACTGCTGATGAAAGt                                                                         >  1:237785/1‑149 (MQ=255)
                                                                         cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGGAAACGAAGAAGTCATCAACCTGCCGATGAAAGTGATCg                                                                    >  1:16799/1‑149 (MQ=255)
                                                                         cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTGCGGTGCGGGTTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCg                                                                    >  1:781136/1‑149 (MQ=255)
                                                                            gCAGGCTTTGCTGGCTGGCTGGGTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccc                                                                 <  1:263482/149‑1 (MQ=255)
                                                                             cAGGCATCGGTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCCGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAAAAAACATCAACCTGCTGATGAAAGTGATCGcccc                                                                <  2:908892/149‑1 (MQ=255)
                                                                                   tCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAccc                                                          <  1:907805/149‑1 (MQ=255)
                                                                                   tCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTAATCAACCTGCTGATGGAAGTGATCGCCCCCTAccc                                                          >  2:232613/1‑149 (MQ=255)
                                                                                       tggctggctggctggcGGACTATCCGCACCTGGCTATTTTACGAACATTGCCGAAGCCTGTTGCTCTGGCGGGCTTTCGTCGCGGGTTTCGGCTGGCAAACGAAGCAGCCATCAACCTGCTGTTGAAAGTGATCGCCACCTAGCCGctc                                                      >  2:414082/1‑149 (MQ=255)
                                                                                           tggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                                  >  1:511587/1‑149 (MQ=255)
                                                                                                 gctggcGGAATATCCGCACCTGCCCATTTGACTCACGCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCGCTCGACGCCgg                                            <  2:198044/149‑1 (MQ=255)
                                                                                                                gCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCGTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGGGATCGCCCCCTACCCGCCCCCGACGCCGGTTGCCCACATTGCgg                             >  2:393774/1‑149 (MQ=255)
                                                                                                                         tATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                    >  1:101937/1‑149 (MQ=255)
                                                                                                                          aTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGtt                   <  2:823923/149‑1 (MQ=255)
                                                                                                                              tACGCACACTGTCGAAAACTTTTGCCCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGc               <  1:504559/149‑1 (MQ=255)
                                                                                                                                 gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca            >  1:797866/1‑149 (MQ=255)
                                                                                                                                          cGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTTATCAACCTGCTGATGAAAGGGATCGCCCCCTCCCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtc   <  2:126913/149‑1 (MQ=255)
                                                                                                                                           gAAAACTCTTGCGCTGGCTGGGTTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcg  <  2:586790/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCG  >  NC_000913/2092897‑2093184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑