Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,501,610 G→A 5.6% L161L (CTG→CTA ypdC → putative DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,501,6100GA 5.6% 213.4 / 3.1 71L161L (CTG→CTAypdCputative DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base A (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

CACCACGTCGTTGTTGCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGC  >  NC_000913/2501463‑2501758
                                                                                                                                                   |                                                                                                                                                    
caccacGTCGTTGTTGCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTtgctgc                                                                                                                                                     <  2:759075/149‑1 (MQ=255)
caccacGTCGTTGTTGCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTtgctgc                                                                                                                                                     >  2:247574/1‑149 (MQ=255)
      gtcgtTGTTGCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCttt                                                                                                                                               <  1:250187/149‑1 (MQ=255)
          ttgttgCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCg                                                                                                                                           >  2:1336065/1‑149 (MQ=255)
          ttgttgCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCg                                                                                                                                           >  1:1160525/1‑149 (MQ=255)
          ttggtgCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCg                                                                                                                                           <  2:226913/149‑1 (MQ=255)
             ttgCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGCAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGATGTTAAGTTTGCTGCATCTTTGCCGTAg                                                                                                                                        >  2:696813/1‑149 (MQ=255)
             ttgCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTac                                                                                                                                        >  2:1446147/1‑148 (MQ=255)
               gCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCg                                                                                                                                      >  2:932261/1‑149 (MQ=255)
                 agccTGCTCCCCAGATCCAGCCTGCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCgtg                                                                                                                                    <  1:265616/147‑1 (MQ=255)
                     tGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCCTCTTTGCCGTAGCggggtgg                                                                                                                                >  2:1009799/1‑148 (MQ=255)
                     tGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGa                                                                                                                                >  2:1181033/1‑149 (MQ=255)
                      gCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGaa                                                                                                                               <  2:1262554/149‑1 (MQ=255)
                      gCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGaa                                                                                                                               <  2:1262727/149‑1 (MQ=255)
                          gCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATg                                                                                                                           <  1:1221950/149‑1 (MQ=255)
                           ccAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATgc                                                                                                                          >  2:636037/1‑149 (MQ=255)
                                tCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCACTCCTCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGccgccg                                                                                                                     <  1:1425448/149‑1 (MQ=255)
                                 ccggCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGTCCTCACCCTTCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGGGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCgg                                                                                                                    <  2:74302/146‑1 (MQ=255)
                                 ccAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCgg                                                                                                                    >  2:1290361/1‑149 (MQ=255)
                                 ccAGCTTCCCAGCCTGCAACGCGGGGAAGGTGAAGCGATGCTTACCGCCCTCCCCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTGGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCgg                                                                                                                    <  2:595424/149‑1 (MQ=255)
                                        ccccGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTc                                                                                                             <  2:1358696/149‑1 (MQ=255)
                                         ccAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCg                                                                                                            <  2:1007097/149‑1 (MQ=255)
                                          cAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGc                                                                                                           >  1:1277866/1‑149 (MQ=255)
                                           aGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCa                                                                                                          <  1:1246177/149‑1 (MQ=255)
                                                gCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgc                                                                                                     >  2:1324547/1‑149 (MQ=255)
                                                 cAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTACATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgcg                                                                                                    >  1:607596/1‑149 (MQ=255)
                                                 cAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTACATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgcg                                                                                                    >  1:607588/1‑149 (MQ=255)
                                                 aaaCGCGGTGACGGTGAAGCGATGCTTACCGCCCTCACCAATCTTAGCCGATCGACGCTGGAGCAAAATATCATTCAGCCCCTGGTGTTGAGTTTGCTGCCTCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcggc                                                                                                    >  2:440709/2‑147 (MQ=255)
                                                  aaCGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgcgc                                                                                                   >  2:1131168/1‑149 (MQ=255)
                                                   aCGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgcgcg                                                                                                  >  1:618497/1‑149 (MQ=255)
                                                   aCGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCcgcgcg                                                                                                  >  1:1145803/1‑149 (MQ=255)
                                                      cgGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCgcggcg                                                                                               <  2:1383576/149‑1 (MQ=255)
                                                      cgGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCgcggcg                                                                                               <  2:641717/149‑1 (MQ=255)
                                                          gaaggtgaagCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGAttt                                                                                           >  1:93791/1‑149 (MQ=255)
                                                           acggggaagCGATGCCTACCGCCCTCACCCCTCTTCGCCGTGCGCCTCTGGAGCAAAATCTCCTTCAGCCGCTGGTGGTAAGTTGGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCACCTGGCAATTCGCAGCCGCGCGGCGAtttt                                                                                          <  1:654095/144‑1 (MQ=255)
                                                              gtgaagCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTtctc                                                                                       <  1:932261/149‑1 (MQ=255)
                                                                   gCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATca                                                                                  >  1:104460/1‑149 (MQ=255)
                                                                    cGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATcac                                                                                 <  2:427761/149‑1 (MQ=255)
                                                                     gATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATcaca                                                                                <  1:35868/149‑1 (MQ=255)
                                                                       tGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGc                                                                              >  2:254973/1‑149 (MQ=255)
                                                                       tGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGc                                                                              >  1:743112/1‑149 (MQ=255)
                                                                       tGCATACAGCTCTCACCCATCTTAGCCGTTCGCCACGGGAGCAAAATATCATTCCGCCGCTGGTGGTAAGTTTGCTGCATCTTTGACGTAGCGTGGTGAACATGCTGCCGGCCAATTCGCAGCCGCGCGCCGATTGCCTCTATCCCAGc                                                                              >  2:534334/1‑149 (MQ=255)
                                                                        gCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCa                                                                             <  2:1015194/149‑1 (MQ=255)
                                                                         cTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCCGCGATTTTCTCTATCACCGcct                                                                            >  1:123521/1‑147 (MQ=255)
                                                                           tACCGCCCTCACCCCTCTTGGCCGTTCGCCGCTGGAGCAGCATGTCCTTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGTCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCAttt                                                                          <  2:308604/149‑1 (MQ=255)
                                                                           tACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCAttt                                                                          <  1:1012190/149‑1 (MQ=255)
                                                                           tACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCAttt                                                                          <  1:939392/149‑1 (MQ=255)
                                                                            aCCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTg                                                                         >  2:292482/1‑149 (MQ=255)
                                                                                cccTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCCCTATCACAGCATTTGTAAc                                                                     >  2:328174/1‑149 (MQ=255)
                                                                                   tCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTgg                                                                  >  2:767729/1‑149 (MQ=255)
                                                                                       ccATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTc                                                              >  1:1235479/1‑149 (MQ=255)
                                                                                        cATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAGTTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCa                                                             >  1:59061/1‑149 (MQ=255)
                                                                                         aTCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAg                                                            <  1:1165814/149‑1 (MQ=255)
                                                                                         aTCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAg                                                            <  1:1165544/149‑1 (MQ=255)
                                                                                          tCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAgg                                                           <  1:51905/149‑1 (MQ=255)
                                                                                            ttAGCCTGTCGCCGCTGGCGCAGAATATCATTCAGGCGCTGGTGTTACGTTTGCGGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGAt                                                         <  2:549668/149‑1 (MQ=255)
                                                                                                  gTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTAt                                                   <  1:503401/149‑1 (MQ=255)
                                                                                                             gAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCt                                        >  2:1051340/1‑149 (MQ=255)
                                                                                                                  aaaTATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCAccc                                   >  1:1223933/1‑149 (MQ=255)
                                                                                                                     tatCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATAGGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTGGTAACTGGGTTCAGGATGATTATGCCCCGCCGCTCACCcgcg                                >  2:937195/1‑149 (MQ=255)
                                                                                                                      atCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTTGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCga                               <  2:500105/149‑1 (MQ=255)
                                                                                                                      atCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCga                               >  2:1364436/1‑149 (MQ=255)
                                                                                                                        cATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCgaga                             <  2:1160525/149‑1 (MQ=255)
                                                                                                                        cATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCgaga                             <  1:1336065/149‑1 (MQ=255)
                                                                                                                          ttCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGc                           >  1:1183305/1‑149 (MQ=255)
                                                                                                                            cAGCCGCTGGTGTTAAGTTTGCTACATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGt                         <  2:607596/149‑1 (MQ=255)
                                                                                                                            cAGCCGCTGGTGTTAAGTTTGCTACATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGt                         <  2:607588/149‑1 (MQ=255)
                                                                                                                              gCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTgg                       <  1:319171/149‑1 (MQ=255)
                                                                                                                              gCCGCTGGTGTTAAGTTTGCTGCATCTTTCCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTgg                       <  2:931960/149‑1 (MQ=255)
                                                                                                                                cGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCCTTTGTAACTGGGTTCAGGACAAGTATGCACAGCCGCTCACCCCCGAGAGCgagagg                     >  1:458553/1‑144 (MQ=255)
                                                                                                                                    ggTGTTAAGTTGGCTGCATCTTTGCCGTAGCGCGGTGAATAGGCCGGCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCCCAGCATTTTTAACTGGGTTCAGGATAATTATGCCCAGCCGCCCACCCGCGAGAGCGTGGCGCAGt                 <  2:393455/149‑1 (MQ=255)
                                                                                                                                        ttAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGCTTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGttttt             >  2:663610/1‑149 (MQ=255)
                                                                                                                                           aGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTaa          <  1:254973/149‑1 (MQ=255)
                                                                                                                                                   gcATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGc  <  1:636037/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CACCACGTCGTTGTTGCATCCTGCTCGCCAGATCCAGCTTCCCAGCCTGCAACGCGGTGAAGGTGAAGCGATGCTTACCGCCCTCACCCATCTTAGCCGTTCGCCGCTGGAGCAAAATATCATTCAGCCGCTGGTGTTAAGTTTGCTGCATCTTTGCCGTAGCGTGGTGAATATGCCGCCGGGCAATTCGCAGCCGCGCGGCGATTTTCTCTATCACAGCATTTGTAACTGGGTTCAGGATAATTATGCCCAGCCGCTCACCCGCGAGAGCGTGGCGCAGTTTTTTAATATCACGC  >  NC_000913/2501463‑2501758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: