Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,915 A→G 6.2% intergenic (+88/‑64) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,9150AG 6.2% 292.7 / 3.7 98intergenic (+88/‑64)ghxP/yjcEguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (43/48);  new base G (4/3);  total (47/51)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATC  >  NC_000913/4279767‑4280062
                                                                                                                                                    |                                                                                                                                                   
cGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaa                                                                                                                                                     >  1:1284415/1‑149 (MQ=255)
  gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAGTGGCTGCCCGTTTTTATTTTCTCCGCACATCGTGTGAGCTTTTGCGATATTCTGaaaaa                                                                                                                                                   >  1:1307017/1‑149 (MQ=255)
  gTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGGGCTTTTGCGAGATTCTGaaaaa                                                                                                                                                   >  1:158418/1‑149 (MQ=255)
     cGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                                                                                                <  1:1275020/149‑1 (MQ=255)
       gTCGTTATCGTCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATga                                                                                                                                              <  1:804244/149‑1 (MQ=255)
       gTCGTTATCGCTGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAGACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAGAAAAtaa                                                                                                                                              >  2:967402/1‑147 (MQ=255)
       gTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATCTTCTGAAAAAAATga                                                                                                                                              >  2:1204481/1‑149 (MQ=255)
        tCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATgag                                                                                                                                             >  2:1038944/1‑149 (MQ=255)
         cGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAACAATgaga                                                                                                                                            >  1:81752/1‑149 (MQ=255)
            tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAtt                                                                                                                                         >  2:1166542/1‑149 (MQ=255)
            tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATCCTGAAAAAAATGAGAAtt                                                                                                                                         >  2:637446/1‑149 (MQ=255)
             aTCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTc                                                                                                                                        <  1:970795/149‑1 (MQ=255)
              tCGCCGTGGCGGTGGTGACCTTCTATGCGGGTGGCTGGGCTACCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAACGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCa                                                                                                                                       <  1:846811/149‑1 (MQ=255)
                 ccGTGGCGCTGGTGACCTTCTATGCGGGTGGGTTAACTAACTAAGCTTTCATCGGATTCTGAAACCGGTTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACCTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGc                                                                                                                                    >  2:317126/1‑149 (MQ=255)
                   gTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCAt                                                                                                                                  <  2:1191054/149‑1 (MQ=255)
                   gTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCAt                                                                                                                                  <  2:1166156/149‑1 (MQ=255)
                        gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGt                                                                                                                             >  1:765444/1‑149 (MQ=255)
                        gcTGGTCACCTACAATGGGGATGGCTGGGCTATCTAATCTTTCGTCGGCTTCTGAAAGCGGGTGGCAATGCCCGCCCGTCTTAATTTTATCCGCACATTGTGTTAGTTTTTGAAAAAAAAAAAAAAAAATGAGAACTCAGGCATAACGt                                                                                                                             <  2:885618/149‑1 (MQ=255)
                                ccTTCTCGGCCGGTGGCTGGGCTATCTAATCTTTCCTCGGCTTCTGAAAACGGTGGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                                                                                                                     <  2:1174638/149‑1 (MQ=255)
                                ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                                                                                                                     <  1:787368/149‑1 (MQ=255)
                                ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCCCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                                                                                                                     <  1:120816/149‑1 (MQ=255)
                                 cTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                                                                                                                    >  1:1359733/1‑149 (MQ=255)
                                  ttCTATGCGGGTGGCTGGGCTATCTAATCTTTCCTCGGCTTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                                                                                                                   <  2:1164818/149‑1 (MQ=255)
                                  ttCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGa                                                                                                                   >  2:1227860/1‑149 (MQ=255)
                                   tCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCCGTGGCTGCCCGTTTTTATTTTCTCCGCGCATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAGTTCAGGCATCACGTCTTTCGCCGAt                                                                                                                  >  1:1374555/1‑149 (MQ=255)
                                   tCTAGGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGAt                                                                                                                  <  1:117288/149‑1 (MQ=255)
                                    cTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAGAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATc                                                                                                                 >  2:939877/1‑149 (MQ=255)
                                          gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaa                                                                                                           <  1:757104/149‑1 (MQ=255)
                                           ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaa                                                                                                          >  1:551836/1‑149 (MQ=255)
                                             tGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCACCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTATCAGATCATaaaagac                                                                                                        >  2:978555/1‑146 (MQ=255)
                                                cTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATGTTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATGAACAACATc                                                                                                     >  1:1206218/1‑149 (MQ=255)
                                                 tGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCg                                                                                                    <  2:695247/149‑1 (MQ=255)
                                                        tCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg                                                                                             >  1:1050880/1‑149 (MQ=255)
                                                         cTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAgg                                                                                            <  1:1265699/149‑1 (MQ=255)
                                                          tAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCGGCCCGTTTTTATTTTGTACGCAGATTGTGTGAGCTTTTGCGATATTATGAAAAATATGAGTATTCAGGAATAACATCTTTCTAAGATCATAAAAAAAATCTCAAAAAggg                                                                                           >  2:82866/1‑149 (MQ=255)
                                                           aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGACTTCAGGCATAACGTCTTTCTCAGATCATAACAAACATCGCAAACAGGGa                                                                                          >  2:9148/1‑149 (MQ=255)
                                                            aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACCTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATAAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAACCAgggca                                                                                         >  2:898389/1‑147 (MQ=255)
                                                            aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAGAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                                                                                         <  1:1024760/149‑1 (MQ=255)
                                                            aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                                                                                         <  1:1166542/149‑1 (MQ=255)
                                                               tttCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                                                                                      >  1:1431095/1‑149 (MQ=255)
                                                                    tCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGGAAAAAATGAGGATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACGGGGAACGCGTGGa                                                                                 >  1:733393/1‑149 (MQ=255)
                                                                     cGGATTCTGAAAACGGGTTGCAATGGCTGCTCGTTTTTATTTTATCCGCACAGTGTGTGAGCTTTTGCGATAATAAAAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGaa                                                                                <  2:1129603/149‑1 (MQ=255)
                                                                     cGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGaa                                                                                <  1:1245384/149‑1 (MQ=255)
                                                                          gcTGTAAACGGGTGGCAATTGCTGGCCGTTTTTATTTTGTCCGCTCATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtctt                                                                           <  2:845947/148‑1 (MQ=255)
                                                                           cTGGAAACGGGTGGGAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGGGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc                                                                          <  2:1448889/149‑1 (MQ=255)
                                                                           cTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc                                                                          <  1:232215/149‑1 (MQ=255)
                                                                           cTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc                                                                          <  2:911109/149‑1 (MQ=255)
                                                                            tGAAAAGGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttct                                                                         <  1:70988/149‑1 (MQ=255)
                                                                            tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGGTATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCCGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttct                                                                         >  1:606376/1‑149 (MQ=255)
                                                                            tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttct                                                                         >  1:175593/1‑149 (MQ=255)
                                                                             ggaaaCGGGTGGCAATGGTTGGCCGGTTTTATTTTCCCCGCACATTGTTTGAGCTTTTGCGATAATCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt                                                                        <  2:844285/147‑1 (MQ=255)
                                                                             gAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt                                                                        <  1:451955/149‑1 (MQ=255)
                                                                              aaaaCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCttcttc                                                                       >  2:853548/1‑149 (MQ=255)
                                                                               aaaCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACGTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAACCttcttcc                                                                      >  2:609322/1‑148 (MQ=255)
                                                                                    ggctgcgaTGGCTGCCCGTTTTTATTTTCTCCGGACATTGTGTGAGTTTTTGAGATATTCAAAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATa                                                                 <  2:292521/142‑1 (MQ=255)
                                                                                     gTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATAc                                                                <  2:542714/149‑1 (MQ=255)
                                                                                         cactGGGTGCCCGCTTTTATGTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGAt                                                            <  2:772991/146‑1 (MQ=255)
                                                                                         cAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGAt                                                            >  1:1372855/1‑149 (MQ=255)
                                                                                         cAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAACCTTCTTCACCATACTGAt                                                            >  1:1177972/1‑149 (MQ=255)
                                                                                           aTGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGGGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATaa                                                          <  2:180044/149‑1 (MQ=255)
                                                                                            gggcggcCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAAt                                                         <  2:921722/148‑1 (MQ=255)
                                                                                               cTGCCCGTTTATATATTCTCCGCACATTGTGTGAGCTTTTGCGATCTTCTGAAAAAAATGAGAATTCAGGCATACCGTCTTTCTCAGATCATAAAAAACATCGCAAACGGGGAACGCAGGGAAATCTTCTTCCCCATACTGATAATGAc                                                      >  2:174323/1‑149 (MQ=255)
                                                                                                tGCCCGTTTTTTTTTTTTTTTTACATTGTGTGAGCGTTTGCGATATTCTGATGAAGATGTGGATTCCGGCATAATGTCTTTCTCAGATCATCCAAAAGATTTCTTTTTTTGAGCGCATGGAAATCTTCTTCACCATACTGATAAttaac                                                     >  2:1321277/1‑145 (MQ=255)
                                                                                                tGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGAcc                                                     >  1:102723/1‑149 (MQ=255)
                                                                                                tGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGAcc                                                     <  1:1193568/149‑1 (MQ=255)
                                                                                                    cGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAGAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCg                                                 <  1:939877/149‑1 (MQ=255)
                                                                                                     gTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGt                                                >  1:818261/1‑149 (MQ=255)
                                                                                                     gTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGt                                                <  1:929108/149‑1 (MQ=255)
                                                                                                      tttttATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTg                                               >  1:1173396/1‑149 (MQ=255)
                                                                                                      tttttATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTg                                               <  2:717363/149‑1 (MQ=255)
                                                                                                        tttATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGt                                             >  2:535147/1‑149 (MQ=255)
                                                                                                          tATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGtct                                           >  2:709553/1‑149 (MQ=255)
                                                                                                           aTTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAACCTTCTTCACCATACTGATAATGACCCTCGTGGtctc                                          <  2:1254504/149‑1 (MQ=255)
                                                                                                           aTTTGCTCCGCACATTGTGTGAGCTTTTGCGATATTATGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGtctc                                          <  1:967402/149‑1 (MQ=255)
                                                                                                            ttttCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCCCGTGGTCTCg                                         >  1:596718/1‑149 (MQ=255)
                                                                                                             tttCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGc                                        >  1:903320/1‑149 (MQ=255)
                                                                                                             tttCTCCGCACATTGAGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTAACCATCCTGATAATGACCCCCGTGGGCTCGc                                        >  2:808458/1‑149 (MQ=255)
                                                                                                                  ccGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATAGCAAACAGGGAACGCATGGAAATCTTCTTCACAATGCTGCTATTGGCGTCGGTGGTCTCGccggcc                                   >  1:1418586/1‑144 (MQ=255)
                                                                                                                   cGCACATTGTGTGAGCTTTTGCGATATTCTGCAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAACCAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCg                                  <  1:978555/149‑1 (MQ=255)
                                                                                                                    gCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCgg                                 >  2:1444460/1‑149 (MQ=255)
                                                                                                                       cgttGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGggt                              <  2:32340/147‑1 (MQ=255)
                                                                                                                       aaTTGTGTGGGCTTTGGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGggt                              <  1:317126/148‑1 (MQ=255)
                                                                                                                        aTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGCATTCATGCATAACGTCTTGCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGggtg                             <  2:158418/149‑1 (MQ=255)
                                                                                                                            ggttaGCTTTTGCGATATTCGGAAAAAAATGAGAAGTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCa                         <  2:1177537/145‑1 (MQ=255)
                                                                                                                              tgAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACt                       >  1:881429/1‑149 (MQ=255)
                                                                                                                              tgAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCCCGTGGTCTCGCTGTCCGGGGTGGTCCCt                       >  2:582352/1‑149 (MQ=255)
                                                                                                                               gAGCTTTTGGGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTc                      <  2:1284415/149‑1 (MQ=255)
                                                                                                                               gAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTc                      >  1:674631/1‑149 (MQ=255)
                                                                                                                                 gCTTTTGCGATTTTCTGAAAAAAATGAGACTTGAGGCATAACGTCTTTCTCAGATCATAAAACACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCgt                    <  2:892555/149‑1 (MQ=255)
                                                                                                                                  cTTTTGCTATATTCTGAAAAAAATGAGCATTCAGGCATCACGTCTTTCGCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCgtg                   <  2:1149703/149‑1 (MQ=255)
                                                                                                                                   ttttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCgtgt                  >  2:563255/1‑149 (MQ=255)
                                                                                                                                   ttttGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAAAATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCgtgt                  >  1:344816/1‑149 (MQ=255)
                                                                                                                                      tGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCAt               <  1:531789/149‑1 (MQ=255)
                                                                                                                                        cGATATTCTGACAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGc             <  2:1285792/149‑1 (MQ=255)
                                                                                                                                               cTGAAGAAAGTGGGAATTCAGGCATAGCGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCa      <  2:932685/149‑1 (MQ=255)
                                                                                                                                               cTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCa      <  1:230391/149‑1 (MQ=255)
                                                                                                                                                 gAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGa    >  1:1232045/1‑149 (MQ=255)
                                                                                                                                                  gaaaaaaTGAGAATTTAGGCATTACGTCTTTCGCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGAt   <  2:1092964/148‑1 (MQ=255)
                                                                                                                                                   aaaaaaTGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATc  <  2:1431095/149‑1 (MQ=255)
                                                                                                                                                   aaaaaaCGAGAATGCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAGGCAGGGAACGCATGGAAATCTTCGTCACCATACTGATAATGCCCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATc  <  1:990845/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGACCCTCGTGGTCTCGCTGTCCGGGGTGGTCACTCGTGTCATGCCCTTTCAGATC  >  NC_000913/4279767‑4280062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: