New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1572380	38 (0.520)	4 (0.060)	4/266	NT	10.0%	intergenic (‑335/+27)	gadB/pqqL	glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase
	?	NC_000913	= 1572395	35 (0.500)					intergenic (‑350/+12)	gadB/pqqL	glutamate decarboxylase B, PLP‑dependent/putative periplasmic M16 family zinc metalloendopeptidase

TTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGC  >  NC_000913/1572232‑1572526                                                                                                                                                     |                                                                                                                                                    ttATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGACCGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCG                                                                                                                                                     <  1:437503‑M1/149‑1 (MQ=255)  tATTCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGc                                                                                                                                                    <  2:436691/149‑1 (MQ=255)       ggTTATTTTTATGCGCGTTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTCCTTGATTATTATTTTGAACGCATTTATATAATTATTACATAAAAATATCCGATATTGCTAAAGTCCCCGCCAAca                                                                                                                                               >  2:998675/2‑148 (MQ=255)          tATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑Atg                                                                                                                                           >  1:251754/1‑149 (MQ=255)            tttttATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑Atgtg                                                                                                                                         >  1:1289722/1‑149 (MQ=255)             ttttATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATgtgt                                                                                                                                        <  2:1126893/149‑1 (MQ=255)                   gcgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcgg                                                                                                                                  >  2:1067378‑M2/1‑146 (MQ=255)                   gcgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcgg                                                                                                                                  >  2:993648‑M2/1‑146 (MQ=255)                   gcgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAAACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGtcaacacatcgttggcgg                                                                                                                                   <  1:1204619‑M1/149‑19 (MQ=255)                    cgcATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCAGTGATAAAATTATTACATAAAAATCGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGAcggg                                                                                                                                 >  1:38562‑M2/1‑145 (MQ=255)                       aaaaagtgtCTCCTGACTTAAGACCGAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCt                                                                                                                              >  2:608288/6‑149 (MQ=255)                        ttCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTg                                                                                                                             <  1:482825/149‑1 (MQ=255)                                ccccTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTattatt                                                                                                                     <  1:478930/147‑1 (MQ=255)                                 tcCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCACCGCCAACG‑ATGTGTTGACGGGGCTGTTATTAttt                                                                                                                    >  1:856777/1‑149 (MQ=255)                                  ccTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTAtttt                                                                                                                   >  2:941714/1‑149 (MQ=255)                                    tGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ACGTGTTGAcggggattttagcaatattc                                                                                                                 <  1:902312‑M2/149‑21 (MQ=255)                                     gAATTATCATGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACACATC‑GTTGGtggggattttagcaatattcg                                                                                                                >  2:1033380‑M2/1‑128 (MQ=255)                                       aTTATCCTGTAAAAATCTTACCTTCAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATACAATTATTACATAAAAATAGCGAATATTTCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCaa                                                                                                              <  2:1168400/149‑1 (MQ=255)                                                 aaaaaCCAAACTTTAAAATATCACTAATAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATACAAATAGCGAATATTGCTAAAATCCCCGCATGCG‑AGTTGTTGACGGGACTGTTATTATTTTGGCAATAATACTCCg                                                                                                    <  1:260572/149‑1 (MQ=255)                                                           ccTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGACAATAATACTCCGGTATAAGTAt                                                                                          <  2:881081/149‑1 (MQ=255)                                                              tAAAATATCACTATTAGTACTTGATTATGATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGGTATTATTTTGGCAATAATACTCCGGTATAAGTATTTa                                                                                       >  1:848125/1‑149 (MQ=255)                                                                      cACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGTGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATga                                                                               <  1:674734/149‑1 (MQ=255)                                                                        cTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATgaga                                                                             <  1:1031080/149‑1 (MQ=255)                                                                        cTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATgaga                                                                             <  2:1229481/149‑1 (MQ=255)                                                                            tAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGccaacgatgtgttgacggggattttagcaatattcgctatttttatgtaataattttataattgcgttcaaaata                                                                          <  2:902312‑M1/149‑76 (MQ=255)                                                                            tAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGa                                                                         >  2:562153/1‑149 (MQ=255)                                                                               tACTTGATTATTATTGTGAACCCATTTATAAAAGTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATATTCCGGTATAAGTATTTACCGGATGAGAAAGatat                                                                      <  2:302718/149‑1 (MQ=255)                                                                               tACTTGATTATTATCTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGatat                                                                      <  1:832796/149‑1 (MQ=255)                                                                                 cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACT‑AGGTTTTGAACGGGCCCTGTTCATTGGGGCAATAATACGCCGGGATAAGTATTTACCGGTTGAAAAAAatatgg                                                                    >  2:275968/1‑147 (MQ=255)                                                                                 cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTg                                                                    >  1:1243932/1‑149 (MQ=255)                                                                                 cTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTg                                                                    <  1:334372/149‑1 (MQ=255)                                                                                          tattTTGAACGCAATTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGTCAACA‑CATCGTTGGcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtacta                                                           <  2:902312‑M2/149‑75 (MQ=255)                                                                                           attTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCa                                                          <  2:1040051/149‑1 (MQ=255)                                                                                             tttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCAGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCGgt                                                        >  2:831758/1‑149 (MQ=255)                                                                                              ttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAgtg                                                       <  2:26675/149‑1 (MQ=255)                                                                                              ttGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAgtg                                                       >  2:999595/1‑149 (MQ=255)                                                                                                      aTTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACAtt                                               <  1:183016/149‑1 (MQ=255)                                                                                                            aaaaTTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTAc                                         <  1:608288/149‑1 (MQ=255)                                                                                                             aaaTTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTAcc                                        >  2:708324/1‑149 (MQ=255)                                                                                                                 tattaCATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCa                                    >  1:17769/1‑149 (MQ=255)                                                                                                                   ttaCATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACCTTCTCTACCGTCAtt                                  >  1:120094/1‑149 (MQ=255)                                                                                                                        tAAGAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGttt                             <  1:1037455/149‑1 (MQ=255)                                                                                                                        tAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGttt                             >  1:756437/1‑149 (MQ=255)                                                                                                                           aaaTAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTcaa                          <  2:382017/149‑1 (MQ=255)                                                                                                                                gCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATGATGTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAAtt                     <  1:1067369/149‑1 (MQ=255)                                                                                                                                 cGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTg                    <  1:1249023/149‑1 (MQ=255)                                                                                                                                 cGAATATTGCTAAAATCCCCAccaacgatgtgttgacggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtctgatttttacatgataattc                     >  2:1033380‑M1/1‑21 (MQ=255)                                                                                                                                  gAATATTGCTAAAATCCCCGtcaacacgtcgttggcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtctgatttttacgtgataattca                    <  1:902312‑M1/149‑130 (MQ=255)                                                                                                                                  gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                   <  1:568771/149‑1 (MQ=255)                                                                                                                                  gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                   >  2:623659/1‑149 (MQ=255)                                                                                                                                  gAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGc                   >  2:190404/1‑149 (MQ=255)                                                                                                                                                  cccGtcaacacatcgttggcggggattttagcaatattcgcgatttttatgtaataattttatcactgcgttcaaaataataatcaagtactaatagtgatattttaaggtctgatttttacgtgataattcaggagacacagaatgcg    >  1:38562‑M1/1‑4 (MQ=255)                                                                                                                                                   ccGtcaacacatcgttggcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtctgatttttacgtgataattcaggagacacagaatgcgc   >  2:993648‑M1/1‑3 (MQ=255)                                                                                                                                                   ccGtcaacacatcgttggcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtctgatttttacgtgataattcaggagacacagaatgcgc   >  2:1067378‑M1/1‑3 (MQ=255)                                                                                                                                                   ccGCCAACG‑ATGTGTTGAcggggattttagcaatattcgctatttttatgtaataattttataaatgcgttcaaaataataatcaagtactaatagtgatattttaaggtttgatttttacgtgataattcaggagacacagaatgcgc  <  1:1204619‑M2/149‑132 (MQ=255)                                                                                                                                                   ccGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCAtgc  <  1:1099776/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTATGCTGTTATTTTTATGCGCATTCTGTGTCTCCTGAATTATCACGTAAAAATCAGACCTTAAAATATCACTATTAGTACTTGATTATTATTTTGAACGCATTTATAAAATTATTACATAAAAATAGCGAATATTGCTAAAATCCCCGCCAACG‑ATGTGTTGACGGGGCTGTTATTATTTTGGCAATAATACTCCGGTATAAGTATTTACCGGATGAGAAAGATATTGTTTAACGGCAGTGTTAACATTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGC  >  NC_000913/1572232‑1572526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.