| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 93,173 | A→C | 100% | R587S (AGA→AGC) D3A (GAT→GCT) |
ftsI → murE → |
transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3 UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 93,173 | 0 | A | C | 98.9% | 272.1 / ‑5.1 | 92 | R587S (AGA→AGC) D3A (GAT→GCT) | ftsI murE | transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3 UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (29/61); minor base T (0/1); total (30/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATAT > NC_000913/93032‑93318 | ctacGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAAt > 2:1274426/1‑149 (MQ=255) tacGGCGGCGCTGTTTCCGCGCCGGCCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACATCGGGCGATAAAAATGAATTTGTGATTAATCAGGGCGAGGGGACAGGTGGCAGCTCGTAAtt < 1:795245/149‑1 (MQ=255) acGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTCAttt > 1:1244446/1‑149 (MQ=255) acGGCGGCGCCGCTTCCGCGCCGGTCTTTGAACGCGGCAGACGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAAttt > 1:1283739/1‑149 (MQ=255) ggcggcGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCGGCCCGTAATTTgc > 2:251728/1‑149 (MQ=255) ggcggcGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGACTAATCAAGGCGAGGGGACAGGTGGCAGCCCGTAATTTgc > 2:666462/1‑149 (MQ=255) gcggcgCCGTTTCCGCGCCGGTCTTTGGTGCCATCCTGGGCGGCGTATTGCGGACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAATGCGAGGGGACAGGTGGCAGCTCGTAATTTgcg < 2:544287/149‑1 (MQ=255) gcgcCGTTTCCGCGCCGGTCTTTGGTGCGATCATGGGCGGCGTATGGCGTACCATGAACATCGAGCCGGACGCGCTGACAGCGGGCGATAAAAATGAATTTGGGGGTAATCAACGCGAGGGGACAGGTGGCAGCTGGGAGTTTGCGGGa > 1:694090/1‑149 (MQ=255) tttCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACcttct < 1:970793/149‑1 (MQ=255) ttCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACcttctg > 2:443010/1‑148 (MQ=255) tCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTg < 1:340909/149‑1 (MQ=255) gcgcCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCGGGTGGTAATTTGGGCGACCTTCTTGCtt > 1:14568/1‑148 (MQ=255) gcgcCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTc > 1:1122850/1‑149 (MQ=255) ccGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGCGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGt < 2:1149808/149‑1 (MQ=255) tggTTTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTg < 2:196994/148‑1 (MQ=255) gggttgtGGGGCCAGCATGGGCGGCGTATTGCGTACCATGAACAGCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTgg < 2:185625/143‑1 (MQ=255) ggTTTTTGGTGCCATCAGGGGCGGCGGATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTgg < 2:687536/149‑1 (MQ=255) ggTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTgg < 2:75495/149‑1 (MQ=255) tttGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGCGGGGACAGGTGGCAGCTCGTAGTTTGCGCGACCTTCCTGCTCCGTGGGTGc > 2:302837/1‑149 (MQ=255) tGCCATCATGGGCGTCGTATTGCGGACCAGGAACATCGAGCCGGATGCGCTGACAACGGGCGAGAAAAATGTATTTGTGATTAATCAAGGCGTGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGAc < 2:438960/149‑1 (MQ=255) tGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGAc < 1:663654/149‑1 (MQ=255) ggCCATCATGTGCCGCGTATTGCGTACCACGAACACCCGGCCGCATGCGCTGACACCGGGCGATAACAATGAATTTGTGCTTAATCATGGCGTGGGGACAGGTGGCCGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGAc < 2:112465/148‑1 (MQ=255) gCCATCATGGGCGGCGTAGTGCGTACCCTGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACg < 2:1165250/149‑1 (MQ=255) atcatGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGGCCTTCTTGCTCCGTGGGTGCCAGACGCAc > 1:1232201/1‑149 (MQ=255) atcatGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCAc > 1:516591/1‑149 (MQ=255) tcatGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCAcc > 1:702647/1‑149 (MQ=255) tcatAGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTGGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAGTTTGCGCGACCTTCTTGCCCCCTGGGTGCCAGCCGGAcc > 2:666565/1‑149 (MQ=255) tGGGGGGCGTATTGCGTACCATGAACATCGAGCCGGAGGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTATTTTGCGCCACCTCCTTGCTCCGTGGGTGCCAGACGCACCTTc < 1:955751/149‑1 (MQ=255) tGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTc < 1:1025832/149‑1 (MQ=255) ggcggcGTACTGCGTCCCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTcgc < 1:1077981/149‑1 (MQ=255) ggcgTATTGCGTACCATGAACATCGAGTCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAg < 1:904051/149‑1 (MQ=255) ggcgTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAg < 1:1269098/149‑1 (MQ=255) ggcgCATTGCGTGCCAGGAACATCGAGCCGGATGCGCTGACAACGGGCGCTAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCGCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAg < 1:909714/149‑1 (MQ=255) aacgTATTGCGTACCTTGAACAGCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGGATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAg < 2:322620/147‑1 (MQ=255) tATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACt > 2:1076157/1‑149 (MQ=255) tATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACt > 2:1270220/1‑149 (MQ=255) tATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGGGCCAGACGCACCTTCGCGAGCACt > 2:337909/1‑149 (MQ=255) aTTGCGTACCATGAGCATCGAGCCGAATGCGCTTACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACCGGTGGAAGCTCGTCATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTg < 1:792204/149‑1 (MQ=255) aTTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGTATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCACCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACTCACCTTCGCGAGCACTg < 2:957226/149‑1 (MQ=255) ttGCTTACCATGAACATCGCGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGc < 1:1154360/149‑1 (MQ=255) cGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCgag < 2:1062664/149‑1 (MQ=255) cGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCgag < 2:988436/149‑1 (MQ=255) cGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCgag < 1:402597/149‑1 (MQ=255) cGTACCAATAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGCAAGTTCGTTATTTGCGAAAACTTCGGGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCgag < 1:309570/149‑1 (MQ=255) ccATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGGGTCAGACGCACCTTCGCGAGCACTTCGGAAGAt > 1:1162486/1‑149 (MQ=255) gAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGaca > 2:295881/1‑149 (MQ=255) aCATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACt < 1:841340/149‑1 (MQ=255) aCAACGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAACTTGCGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACt < 1:593798/149‑1 (MQ=255) tCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGGGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGa > 2:721638/1‑149 (MQ=255) aGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAg > 1:868977/1‑149 (MQ=255) cGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGGGAGATGACACTCGACAGCCg > 2:1094942/1‑149 (MQ=255) ggATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCGGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGGCGCACCTTCGCGAGGACTGCGCGAGATGTTACTCGCCAGCCgt > 1:369764/1‑149 (MQ=255) ggcTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACGCGACAGCCGTGTGGc > 2:484620/2‑149 (MQ=255) gcTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCATCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCt > 1:862126/1‑149 (MQ=255) gcTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACCCTCGACAGCCGTGTGGCt > 1:1058842/1‑149 (MQ=255) cTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGCGTGGccg > 2:321502/1‑147 (MQ=255) gggCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGGGAGATGACACTCGACAGCCGTGTGGCTGCggcgggc < 2:174479/149‑1 (MQ=255) ggCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGTGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGCGGCTGCggcgggcg < 1:1158584/149‑1 (MQ=255) cGATAACAATGAGTGTGTGATTAAGCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGAGGACACTCGACAGCCGTGTGGCTGCGGCGGGCGAt < 1:322504/149‑1 (MQ=255) cGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGAt < 1:1274426/149‑1 (MQ=255) gATAAAATGGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGAtc < 1:594031/149‑1 (MQ=255) aaTGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTg < 1:387434/149‑1 (MQ=255) aaTGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGGAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTg < 1:1198065/149‑1 (MQ=255) attaaTTTGTGATTAAGCAAGGCGAGGGGACAGGTGGCAGCTCATAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGt < 1:190521/146‑1 (MQ=255) aTGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGt < 1:890235/149‑1 (MQ=255) tGAATTGGTGATTAAGCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCCCCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTa < 2:148978/149‑1 (MQ=255) ggAAGTTGTGATCAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTGGCGCGACCTTCTTGCGCCGTGGGTGCCAGAGGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTa < 2:174705/148‑1 (MQ=255) gAATTTGTGACTGATCAAGGCGAGGGGGCAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGGGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAg < 2:1232112/149‑1 (MQ=255) aaTTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGc > 1:333943/1‑149 (MQ=255) aTTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGCCAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCt < 1:838830/149‑1 (MQ=255) tttGTGATTAATCAAGGTGAGGGAGCAGGAGGCAGCTCGTAACTTGCGCGACCTTCCTGCTCCGTGGGTGCCAGACGCCCCTTCGCGAGCACTGCGAGAGATGACACCCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTg < 1:443010/149‑1 (MQ=255) ttGTGATTAATCAAGGCGGGGGGACAGGTGGCAGCTCGTAATTTGCGCGACTTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgt < 2:1122850/149‑1 (MQ=255) ttGCGATTAATCAAGGCGAGGGCACAGGTGGCAGCTCGTAATTTGCGCGACCTTCCTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCATTGTAGCTgt < 2:14568/149‑1 (MQ=255) tgtgATTAATCAGGGCGAGGGGACAGGTGGCAGCTCGCAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgta < 2:9360/149‑1 (MQ=255) tgtgATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgta < 2:1286495/149‑1 (MQ=255) tgATTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTgtagt < 2:333943/149‑1 (MQ=255) actAATCAAGGCGAGGGGACAGGTGGCAGCTCGCAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGGCGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGtagtag < 1:377415/147‑1 (MQ=255) aTTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGtagtag < 1:376746/149‑1 (MQ=255) aTTAATCAAGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCAGTGTTTTAGCGGCGGGCGATCTCTTTGTAGCTGtagtag < 1:635469/149‑1 (MQ=255) aaTCAAGGCGAGTGGACAGGTGGCAGCCCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGtc < 1:352090/149‑1 (MQ=255) aaGGCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCAACCGCCGTGTGGCTGCGGCGGGCGATCTCCTTGTGGCGGTAGTAGGtcatca > 2:139384/1‑149 (MQ=255) gCGAGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGc < 1:1015937/149‑1 (MQ=255) gggggACGGGGGGCAGCTCGTAGTTTGCGCGACCTCCTTGCTCCGTGGGTGCCAGACGCCCCTTCGCGCGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa < 2:770195/148‑1 (MQ=255) aGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa < 1:1009778/149‑1 (MQ=255) aGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa < 1:23012/149‑1 (MQ=255) aGGGGACAGGTGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGACAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGa < 1:696439/149‑1 (MQ=255) aGGGGACAGGCGGCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCGCTTTGTAGCTGTAGTAGGTCATCAGGCGGa < 2:125489/149‑1 (MQ=255) ggCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGat < 1:781715/149‑1 (MQ=255) ggCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGat < 1:1215675/149‑1 (MQ=255) ggCAGCTCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGat < 1:1177501/149‑1 (MQ=255) gCAGCTCGTAATTTGCGCGACCTTCTTCCCCCGTCGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGCCTGCGGCGGGCGCTCTCTCCGCACCTGCAGCAGGCCACCAGCCCGCCGGGCGTCGcta > 2:171181/1‑149 (MQ=255) cAGCTCGTAAGTTGCGCGACCTTGTTGCTCCGTGGGGGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACGCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGatat < 1:908001/149‑1 (MQ=255) | CTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACCATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATAT > NC_000913/93032‑93318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |