Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,305,011 A→G 7.4% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,0110AG 7.4% 276.1 / 6.2 107intergenic (+620/+95)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (40/57);  new base G (5/4);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCG  >  NC_000913/2304868‑2305158
                                                                                                                                                |                                                                                                                                                   
tAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATc                                                                                                                                                <  2:806819/149‑1 (MQ=32)
 aGGTCGGATAAGGCGGGTACGCCGCATCCGACCTCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCt                                                                                                                                               <  1:1079791/149‑1 (MQ=16)
   gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAAc                                                                                                                                                   <  2:292194/143‑1 (MQ=32)
          aaGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccggg                                                                                                                                      >  1:259338/1‑146 (MQ=37)
               ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCaa                                                                                                                                                    <  2:610104/130‑1 (MQ=31)
                     gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTGACGCCGCATCCAACATCTACCGCCCGCGCCGGTTGCCt                                                                                                                           >  2:1072723/1‑149 (MQ=255)
                     gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                                           >  1:541294/1‑149 (MQ=38)
                      ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTg                                                                                                                          >  2:804290/1‑149 (MQ=38)
                                  tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGATGTGAAGTGCTCCACCCCGTAGGATGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                              >  1:307554/1‑149 (MQ=255)
                                    tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACCTCTACCGCCCGAGCCGGTTGCCTGATGGGCCGCTcgcg                                                                                                            >  1:237059/1‑149 (MQ=11)
                                    tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGTTGCGACGCTGgcg                                                                                                            >  1:274366/1‑149 (MQ=37)
                                    tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGCTGCGCCGCTGgcg                                                                                                            >  2:640339/1‑149 (MQ=255)
                                      ccGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                          <  1:405791/148‑1 (MQ=39)
                                      aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCCACATCTAACGCCCGAGCCGGTTGCCTGATGCGGCCCTGGcgcg                                                                                                          >  2:86825/1‑149 (MQ=255)
                                       cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCTCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGGGACGCTGGCGCGt                                                                                                         <  1:884742/149‑1 (MQ=255)
                                       cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                         <  1:1193959/149‑1 (MQ=39)
                                        gtccgaGCCGGTTCCCTGACGCCACGCTGCCGCGCCTTTTCCGGCCCACACCGCTGTGAAGTGCCCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                        <  2:625369/144‑1 (MQ=11)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                                                                                                                         <  2:1141654/116‑1 (MQ=18)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                                                                                                                         <  2:1141842/116‑1 (MQ=21)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                                                                                                                         <  2:1180080/116‑1 (MQ=21)
                                        gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATGCGACATCTAAGGCCCGGGCAGGTTGGCTGTTGCGACGCTGGCGCGtg                                                                                                        >  1:202622/1‑148 (MQ=11)
                                            aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGCTTGCCTGATGCCACGCCGGCGCGTCTTAt                                                                                                    >  2:984883/1‑149 (MQ=255)
                                             aGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                   <  1:103473/149‑1 (MQ=37)
                                                cGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTTGGGTAACGCGGTTACGCCGCCTCCAACCTCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGGCCGTCTTATCAGc                                                                                                >  2:1017295/1‑149 (MQ=11)
                                                cGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCTGGTTGTCTGATGCGACGCTGGCGCGTCTTATCAGc                                                                                                <  1:804266/149‑1 (MQ=255)
                                                 ggTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGAAGCGACGCTGGCGGGTCTTATCAggc                                                                                               >  2:84116/1‑147 (MQ=2)
                                                     gCCTGATGCGCCGATGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACg                                                                                           <  1:459968/149‑1 (MQ=255)
                                                      ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCTACGc                                                                                          >  1:588156/1‑149 (MQ=255)
                                                      ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                                          <  1:433717/149‑1 (MQ=255)
                                                      ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                                          >  2:148103/1‑149 (MQ=255)
                                                      ccTGATGCGACGCCCCCGCGTCGCATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                                          <  1:147410/149‑1 (MQ=11)
                                                      ccTGATGAGACGCGGCCGCGTCTCATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                                          <  2:62432/149‑1 (MQ=255)
                                                       cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACGTCTAACGCCCGAGCCGGTTGTCTGATGCGACGCAGGCGCGTCTTATCAGCATACGcc                                                                                         >  1:411437/1‑149 (MQ=255)
                                                        tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGGAGCTGGCGCGTCTTATCAGCATACGCca                                                                                        >  1:907115/1‑149 (MQ=255)
                                                        tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCca                                                                                        <  1:698083/149‑1 (MQ=255)
                                                              gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGCTGCGACGCAGACGCGTCTTATCAGCATAAGACACATCCg                                                                                  >  2:1166501/1‑149 (MQ=14)
                                                              gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCg                                                                                  >  2:385964/1‑149 (MQ=255)
                                                               ccgttGGCGCGGCAGATCAGGCCTGCACCGCTGTGATGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCgg                                                                                 <  1:1109763/145‑1 (MQ=14)
                                                               aCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTTGCGCGTCTTATCAGCATACGCCACATCCgg                                                                                 >  2:1035524/1‑149 (MQ=255)
                                                                  cTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGa                                                                                                                         >  2:48341/1‑106 (MQ=40)
                                                                  cTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGa                                                                                                                         <  1:48341/106‑1 (MQ=40)
                                                                  cTGGCGCGCCTTATCACGCCTACACCGCTGTGAAGCGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCAt                                                                              <  2:199791/149‑1 (MQ=255)
                                                                   tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATa                                                                             <  1:543850/149‑1 (MQ=255)
                                                                   tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATa                                                                             <  1:104067/149‑1 (MQ=255)
                                                                   cgccgcgTCTTATCAGGCCCACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATa                                                                             <  1:567175/146‑1 (MQ=255)
                                                                     gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATAcc                                                                           >  1:777284/1‑149 (MQ=255)
                                                                      cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCa                                                                          <  1:338136/149‑1 (MQ=255)
                                                                         gTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGc                                                                       <  1:804290/149‑1 (MQ=255)
                                                                         gTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc                                                                                                                             <  2:710328/95‑1 (MQ=21)
                                                                         gTCTTACCGGGCCCACAACGCCGTGAAGTGCTCCGCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGc                                                                       <  1:352540/149‑1 (MQ=18)
                                                                          tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCt                                                                                                                                               <  2:605823/76‑1 (MQ=38)
                                                                            ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCgg                                                                    >  1:238538/1‑149 (MQ=255)
                                                                            ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCgg                                                                    >  1:310823/1‑149 (MQ=255)
                                                                             tATCAGGCCTACACCGCTCTGACGTGCTCGACCCCGTATGTTCGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGTTTGCCTGATGCGACGCTGCCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGa                                                                   <  2:362519/149‑1 (MQ=18)
                                                                              aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                                                  <  1:228933/149‑1 (MQ=255)
                                                                              aTCAGGCCTACACCGCTGTGAAGCGCTCCGCCGTCGTGCTCGGGCTTCGCGGAATCGCCGCATCCAACAGCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                                                  <  1:297672/149‑1 (MQ=17)
                                                                               tccggCCTACCCCGCTGTGAAGTCCTCCACCCCGTACGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAtg                                                                 <  1:1016927/146‑1 (MQ=18)
                                                                               tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCAGTTACGACGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAtg                                                                 >  2:845483/1‑149 (MQ=255)
                                                                               tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTAGATAAGGCGGTTACGCCGCATCCAACa                                                                                                                                                  <  2:723003/68‑1 (MQ=25)
                                                                               tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTCCCGCCCGAGCCGGTTGCCTGATGTGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAtg                                                                 <  2:699333/149‑1 (MQ=255)
                                                                                   gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCCTCTCCGCATACGCCACATCCGGCATACCATGCCGGCTGTGGc                                                             >  2:3248/1‑149 (MQ=18)
                                                                                      tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCTACGCCCCATCCGGCATGCCATGCCGGGTGTGGCgga                                                          >  2:41810/1‑147 (MQ=14)
                                                                                       ccacCGCTGTCACGTGCCCCCCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTAt                                                         <  2:523949/148‑1 (MQ=255)
                                                                                         acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCa                                                       >  1:746483/1‑149 (MQ=255)
                                                                                         acCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGAt                                                                                                                        <  2:504414/84‑1 (MQ=25)
                                                                                           cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCAtt                                                     >  2:1122892/1‑149 (MQ=255)
                                                                                            gCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTa                                                    <  1:1033724/149‑1 (MQ=255)
                                                                                              tgtgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACa                                                  >  2:1288904/1‑149 (MQ=255)
                                                                                               gtgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACaa                                                 <  1:1232856/149‑1 (MQ=255)
                                                                                               gtgAAGGGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACaa                                                 <  1:84116/149‑1 (MQ=255)
                                                                                                  aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCTACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGc                                              >  2:582392/1‑149 (MQ=255)
                                                                                                    gggCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCaa                                            <  1:41810/147‑1 (MQ=255)
                                                                                                    gTGCTCCCCCCCGTAGGTTGGATAAGGCGGTCACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCACGCCGGATGTGGCGTATCATTACAACGCaa                                            <  1:970658/149‑1 (MQ=255)
                                                                                                     tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAGGGGGCGCATCATTACAAAGCAat                                           >  2:349022/1‑149 (MQ=255)
                                                                                                       cTCCGCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCgg                                                                                 <  2:852756/109‑1 (MQ=11)
                                                                                                       cTCCGCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCgg                                                                                 >  1:852756/1‑109 (MQ=11)
                                                                                                       cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                    <  2:105695/90‑1 (MQ=255)
                                                                                                        tCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATc                                        <  2:1229347/149‑1 (MQ=255)
                                                                                                           aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTAt                                                         >  2:132076/1‑129 (MQ=255)
                                                                                                           aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTAt                                                         <  1:132076/129‑1 (MQ=255)
                                                                                                           aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc                                     <  1:311885/149‑1 (MQ=255)
                                                                                                           aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc                                     <  1:323748/149‑1 (MQ=255)
                                                                                                             cccGTAGGGTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCa                                   <  1:979187/149‑1 (MQ=255)
                                                                                                                cGTAGGTTGGATAAGGCGGTTACGCCGCGTCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATCTGGCGTATCATTACAACGCAATAACCGCCAct                                 >  2:726531/1‑149 (MQ=255)
                                                                                                                 gTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTCTCATTACAACGCAATATCCGCCActt                                >  2:447862/1‑149 (MQ=255)
                                                                                                                              ggCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAATggg                   <  1:1156351/149‑1 (MQ=255)
                                                                                                                                  gTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGCATACGCCACATCCGGCATAACATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCACATTCTTTTTGAACGCGTTga               >  1:975484/1‑148 (MQ=37)
                                                                                                                                     aCGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGt            >  2:880924/1‑149 (MQ=255)
                                                                                                                                     aCGCCGCATCCAACATCTAACGCCCGAACCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGt            <  1:931780/149‑1 (MQ=255)
                                                                                                                                        ccgcATCCAACATCTAACGCCCGAGCCGTTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCACACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGGAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTc         >  1:845480/1‑149 (MQ=255)
                                                                                                                                        ccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTc         >  1:958750/1‑149 (MQ=255)
                                                                                                                                         cgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTGCAACGCCATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCa        >  1:563599/1‑149 (MQ=255)
                                                                                                                                         cgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCa        >  1:545616/1‑149 (MQ=255)
                                                                                                                                          gcATCCAACATCTAACGCCCGGGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa       <  2:268951/149‑1 (MQ=255)
                                                                                                                                          gcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa       <  1:159245/149‑1 (MQ=255)
                                                                                                                                          gcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa       <  2:85485/149‑1 (MQ=255)
                                                                                                                                          gcATCCAACACTCAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCaa       <  2:1024768/149‑1 (MQ=255)
                                                                                                                                           ccgccAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCatc      <  2:187207/146‑3 (MQ=255)
                                                                                                                                           cATCCAACATCTCACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGGGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAAc      <  2:454440/149‑1 (MQ=255)
                                                                                                                                               cAACATCCAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCg  <  2:665270/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCG  >  NC_000913/2304868‑2305158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: