Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,618 T→A 8.1% intergenic (‑246/‑27) glpT ← / → glpA sn‑glycerol‑3‑phosphate transporter/anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,6180TA 8.1% 190.7 / 2.0 69intergenic (‑246/‑27)glpT/glpAsn‑glycerol‑3‑phosphate transporter/anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base A (2/4);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAG  >  NC_000913/2352473‑2352758
                                                                                                                                                 |                                                                                                                                            
aTAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGTGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTaaa                                                                                                                                           >  2:1090636/1‑149 (MQ=255)
     ttaaatGTGACTTGCCTGACCTCTTATTAAATAATATTTACAATATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGTATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACg                                                                                                                                      <  2:583191/149‑1 (MQ=255)
     ttaaatGTGAATATCCACACACATTATAAAATAATATTTACAAAATGTTCACAATGACGCATGAAATCACGTTTCACTTTCGAATTATGCGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACg                                                                                                                                      <  2:825272/149‑1 (MQ=255)
         atGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACt                                                                                                                                  >  1:355507/1‑149 (MQ=255)
           gtgAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTc                                                                                                                                <  2:101603/149‑1 (MQ=255)
           gtgAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAACGACGCATGAAATCACGGTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGATAAAAAATAAAAAACGAACTTc                                                                                                                                <  2:1259371/149‑1 (MQ=255)
             gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCag                                                                                                                              <  1:1015164/149‑1 (MQ=255)
              aagtGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                                                                                                                             <  1:511974/146‑1 (MQ=255)
              aaTTGCCGCACATATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                                                                                                                             <  1:1226750/149‑1 (MQ=255)
                ttGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGTGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCGGAgg                                                                                                                           >  2:1271160/1‑149 (MQ=255)
                  gCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGATAAATAATAAAAAACGAACTTCAGAGGGa                                                                                                                         <  2:727267/149‑1 (MQ=255)
                       acacaTTATTAAATAAGATTTACAAAATGTTCAAACTGACGCACGAAATCACGTTTCACTTTCGAATTCTGAGCGAAGATGCGAACAATTAAACAATGCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACa                                                                                                                    <  1:628718/149‑1 (MQ=255)
                         acaTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAAt                                                                                                                  >  1:1071422/1‑149 (MQ=255)
                         acaTTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAAGTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAAt                                                                                                                  >  1:1011631/1‑149 (MQ=255)
                            ttattaAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTACATGGTAAAAAACGAACTTCAGAGGGATAACCATGaa                                                                                                               >  2:50703/1‑149 (MQ=255)
                            ttattaAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa                                                                                                               <  2:263338/149‑1 (MQ=255)
                            ttattaAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa                                                                                                               >  2:975589/1‑149 (MQ=255)
                            taattaGATAACATTTACAAAATGTTCCAAATGACGCATGAACGCACGTTTCACTTTCGAATTATTAGCGAATATGCTCGAAATGAAACAATTCATGTTTTTACAATGGCTAAATGGTAAAAAACTAATTTCAGAGGGATATTAATgta                                                                                                               <  2:224886/147‑3 (MQ=255)
                             tattaAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATAACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAACCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAAAGAACTTCAGAGGGATCACAATGaaa                                                                                                              >  2:736599/1‑149 (MQ=255)
                                 aaaTAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATAGAAAAAAAGAAAAAAACGAACTTCAGAGGGATAACAATGAAAACTc                                                                                                          <  2:694357/149‑1 (MQ=255)
                                  aaaaaGATTTACAAAATGTACAAAATGACGCATGAAATCACGTAACACCATCGAATTATGAGCGAATAAGCACGAAATCAAACAATTCATGTTTTTACTATAAAAAAAAAAAAAAAAACGAACTTCAGAGGGATAACAATGAAAACTcg                                                                                                         <  2:504155/146‑1 (MQ=255)
                                   aTAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTcgc                                                                                                        >  2:1152845/1‑149 (MQ=255)
                                     aaGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGa                                                                                                      >  2:571188/1‑149 (MQ=255)
                                      caaattACAAAAAGTTCAAAATGACGCAGTAAATCACGTTTCACCTTCGAATTATGAGCGAATATGCACGAAATCAAACAATTCATGTTTTTACTATGAAAAAAAAAAAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGAc                                                                                                     <  2:656153/145‑1 (MQ=255)
                                        aTTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAAAACTGAAAACTCGCAACTc                                                                                                   >  2:1082067/1‑149 (MQ=255)
                                              aaaaTGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACGCGCAATc                                                                                             >  1:1176895/1‑149 (MQ=255)
                                                aaTGTTCACAATGACGCATGAAAACACGTTTCACTTGCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCaa                                                                                           <  1:758617/149‑1 (MQ=255)
                                                aaTGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAAAAATGAAAACTCGCAACTCGCAATCaa                                                                                           >  1:9512/1‑149 (MQ=255)
                                                     tCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGAc                                                                                      <  1:611587/149‑1 (MQ=255)
                                                       aaaaTGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTAACTATGGCTAAATGGAAGAAAACGAACTTCGAATGAAGAACAATGAAAACTCGCGCCTCGCAATCAAGTGagtc                                                                                    >  2:1029413/1‑146 (MQ=255)
                                                        aaaTGACGCATGAAATCACGGTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTg                                                                                   <  1:319720/149‑1 (MQ=255)
                                                            gACGCATGAAATCACGCTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGATGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGAttc                                                                               <  2:694997/149‑2 (MQ=255)
                                                               gCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCa                                                                            <  1:688722/149‑1 (MQ=255)
                                                               gCAGGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCa                                                                            >  2:816873/1‑149 (MQ=255)
                                                                cATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCAt                                                                           <  1:1268146/149‑1 (MQ=255)
                                                                cATGAAATCACGTTTCACTTTCAGATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCAt                                                                           <  2:312282/149‑1 (MQ=255)
                                                                  tGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGTCTAAATGGTAAAAAACGAACTTCAGAAGGATAACAATGAAAACTCGCGACTAGCAATCAAGTGACGTGATTATAATTg                                                                         >  2:226664/1‑149 (MQ=255)
                                                                   gAAATCACGTTTCACCTTCGAATTATGAGCGAATATGCGCGAAATCATACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTgg                                                                        <  1:1115581/149‑1 (MQ=255)
                                                                   gAAATCACGTTGCACTTTCGAATTATGAGCGAATATGCCCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGTCTCGCAATCAAGTGACGTGATTATCATTgg                                                                        <  2:1240713/149‑1 (MQ=255)
                                                                     aaTCACTTTTTACGTTCGAAGTAGAAGCGAATAGGCGCGAAATCAAACAATTCATGTTTTTACTATGGTTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcg                                                                      <  1:12634/149‑1 (MQ=255)
                                                                      aTCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTTTCATTggcgg                                                                     >  1:560550/1‑149 (MQ=255)
                                                                         gcGTTGCTCTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcgg                                                                  <  2:274248/148‑1 (MQ=255)
                                                                                 cTTTCGAATTATGAGTGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACgg                                                          <  1:1090636/149‑1 (MQ=255)
                                                                                  tttCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACggg                                                         >  2:927245/1‑149 (MQ=255)
                                                                                  tttCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACggg                                                         >  2:927231/1‑149 (MQ=255)
                                                                                      gAATTATGAGCGAATATGCGCGAAGTCAAACAATTCATGTGTTTACTATGGCAAACTGGAAAAAAACGATATTCAAAGGCATAACAATGAAAACTCGCCACACGCAATCAAGTGACGTGATTATCATTGGCGTCGGCGCAAcagaggcc                                                     >  2:408976/1‑142 (MQ=255)
                                                                                      gAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATTTTTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACACGCGACTCGCAATCACGCGACGTGATCATCATTGGCGGCGGCGCAACGGGCGcc                                                     >  2:322003/1‑149 (MQ=255)
                                                                                        aTTATGAGCGAATATGTGCGAAATCAAACAATTCATGTTTTTACTATGGCTTGTAGCAAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg                                                   <  1:1271160/149‑1 (MQ=255)
                                                                                        aTTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg                                                   <  1:1027604/149‑1 (MQ=255)
                                                                                         ttATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCggg                                                  >  2:1224709/1‑149 (MQ=255)
                                                                                               gCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc                                            <  1:1143130/149‑1 (MQ=255)
                                                                                                 gAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCcg                                          <  1:1126850/149‑1 (MQ=255)
                                                                                                 gAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCcg                                          <  1:694456/149‑1 (MQ=255)
                                                                                                      tGCGCGAAATCAGACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACt                                     >  1:723713/1‑149 (MQ=255)
                                                                                                       gcgcGAAATCAAACAATTCATGTTTTTTTTTTTTTTTTTTGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACtg                                    >  2:1042385/1‑149 (MQ=255)
                                                                                                         gcgAAATCAAACAATTCATGTTTTTACCATGGCTAAATGGTAAAAAACGAACTTCATAGGGATAACAATGAAAACCCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACtgtg                                  <  1:797559/149‑1 (MQ=255)
                                                                                                         gcgAAAACAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACtgtg                                  <  1:873837/149‑1 (MQ=255)
                                                                                                          cgAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGc                                 >  2:1246609/1‑149 (MQ=255)
                                                                                                             aacgaaaCAAATCCAGTTCTGACTATGGCTAAATGGTAAAAAACGAACCTCAGAGGGATAACAATGCAAACTCGCGCCTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCt                              <  1:1026987/145‑1 (MQ=255)
                                                                                                                 aaaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTgcgc                          >  1:1066225/1‑149 (MQ=255)
                                                                                                                 aaaCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTgcgc                          >  1:218519/1‑149 (MQ=255)
                                                                                                                   aCAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGGGCCGGGATTGCCCGCGACTGTGCCCGGCGCgg                        >  2:1264278/1‑149 (MQ=255)
                                                                                                                          aTGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTgcgc                 >  1:2520/1‑149 (MQ=255)
                                                                                                                                    tATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGGTTTTgg       >  2:766007/1‑149 (MQ=255)
                                                                                                                                       ggCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTAACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTg    >  1:534035/1‑149 (MQ=255)
                                                                                                                                        gcgaaaTGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGa   <  2:112505/146‑1 (MQ=255)
                                                                                                                                        gCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGa   >  2:1125405/1‑149 (MQ=255)
                                                                                                                                        gCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGa   <  1:538013/149‑1 (MQ=255)
                                                                                                                                         cTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAg  <  1:643849/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
ATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAG  >  NC_000913/2352473‑2352758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: