Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,427,541 C→T 8.7% A83A (GCG→GCA)  argT ← lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,427,5410CT 8.7% 156.3 / 4.1 56A83A (GCG→GCA) argTlysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (3/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TTCAGTGAATCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGT  >  NC_000913/2427395‑2427687
                                                                                                                                                  |                                                                                                                                                  
ttCAGTGAATCCCGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGc                                                                                                                                                  <  2:778519/149‑1 (MQ=255)
 tCAGTGAATCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAACGCGACGAAATAATAGCGTCGATTTTTTTCGCt                                                                                                                                                 >  2:748675/1‑149 (MQ=255)
 tCAGTGAATCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCt                                                                                                                                                 >  1:846047/1‑149 (MQ=255)
    gTGAAGCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATGAAACGAGAATCTGCCGCGTCACGCCTCTCGGAGAAGACAACCGCCCGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTc                                                                                                                                              <  1:628304/149‑1 (MQ=255)
         tCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTGTCGGTAATGGAAAGCGACGAAAGAATAGCGTCGCTTTTTTTCGCTTTCGGTGa                                                                                                                                         >  1:429682/1‑149 (MQ=255)
          ccAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCGTGCCGTTGAAAATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGGGAg                                                                                                                                        >  1:1272528/1‑149 (MQ=255)
          ccAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAg                                                                                                                                        >  1:160528/1‑149 (MQ=255)
            ggCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGTGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAggg                                                                                                                                      <  2:365187/148‑1 (MQ=255)
            aGCGTTGGCTGAAGCGCTGCACC‑TTAGCCGCAATCAACCGAGAATCTGCGGCGTACAGCTTGCCGGAGAAGGCACTCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAgggg                                                                                                                                     <  2:767835/149‑1 (MQ=255)
               gTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGAt                                                                                                                                   <  1:919116/149‑1 (MQ=255)
                    cTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCGGGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCCGTGAGGGGATCAgcg                                                                                                                              >  2:976644/1‑149 (MQ=255)
                     tGAATTGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAgcgc                                                                                                                             <  2:53069/149‑1 (MQ=255)
                      gAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGcgcg                                                                                                                            <  1:753003/149‑1 (MQ=255)
                           ggTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCaaa                                                                                                                       <  1:936472/149‑1 (MQ=255)
                                  cTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTTGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTg                                                                                                                >  1:234754/1‑149 (MQ=255)
                                  cTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTTGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTg                                                                                                                >  1:234520/1‑149 (MQ=255)
                                   tttGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTgg                                                                                                               <  1:500560/149‑1 (MQ=255)
                                      ggCCGCACTCAAAGGCGGATGTGCGGCGGACGTCTTGGCGGGGAACGCAATCTCCTGCTGAGCGTTATCGGGAATGGTAACCGATGAAATCATAGCGTCGATTTTTTTCGCTTTCAGTGAGTGGTTCGGCGCGTCAGAGTGACTGGCaa                                                                                                            <  2:642812/149‑1 (MQ=255)
                                         cGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAAccc                                                                                                         <  1:290049/149‑1 (MQ=255)
                                               cAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTAc                                                                                                   <  2:933692/149‑1 (MQ=255)
                                                 aaCGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACAt                                                                                                 <  1:770676/149‑1 (MQ=255)
                                                    gcgaATCTGCGGCGTACAGCTTGTCGAAGAAGACAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTAAGTGAGAGGATCAGCGCGTCAAAGACACTGGCAACCCAGGGACATTTc                                                                                              >  2:140899/3‑149 (MQ=255)
                                                    gagaATCTGCGGCGTACAGCTTGTCGGAGCAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTc                                                                                              >  1:794412/1‑149 (MQ=255)
                                                    gagaATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCCAAGTCACTGGCAACCCAGGTACAGTTc                                                                                              >  1:189581/1‑149 (MQ=255)
                                                    gagaATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTc                                                                                              >  2:195925/1‑149 (MQ=255)
                                                     acaaTCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAATTCACTGGCAACCCAGGTACATTTCa                                                                                             <  1:653797/147‑1 (MQ=255)
                                                          cTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGGAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTTGCTTTCAGTGAGGGGATCAGCGCGGCAAAGTAACTGGAAACCCAGGTACATTTCACCTGc                                                                                        >  2:87076/1‑149 (MQ=255)
                                                           tGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAACGTCGCTTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACCTTTCACCTGCa                                                                                       >  1:1238107/1‑149 (MQ=255)
                                                                 gTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCGGCATCCGtt                                                                                 >  1:1106285/1‑149 (MQ=255)
                                                                   aCAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTTGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTg                                                                               <  2:234754/149‑1 (MQ=255)
                                                                   aCAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTTGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTg                                                                               <  2:234520/149‑1 (MQ=255)
                                                                       cTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGcaca                                                                           >  2:404978/1‑149 (MQ=255)
                                                                        ttGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCCCCTGCATCCGTTTGCACAt                                                                          >  2:634724/1‑149 (MQ=255)
                                                                        ttGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAt                                                                          >  1:311133/1‑149 (MQ=255)
                                                                           tCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACAtctc                                                                       >  1:436184/1‑149 (MQ=255)
                                                                            cGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCg                                                                      <  1:757937/149‑1 (MQ=255)
                                                                             ggAGAAGGCAATCTCCTGCTGACGTTTATAGGTGGTGTAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGt                                                                     >  1:956681/1‑149 (MQ=255)
                                                                              gagaAGGCAATCACCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAGGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTCCACAtatcata                                                                    >  1:934343/1‑146 (MQ=255)
                                                                                   ggCAAGCTCCTGGTGACGTTGATCGGGAATGGAAAGGGACGAAATAATAGCGGCGATTTTTTTCGCTTTCAGTGCGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCga                                                               <  2:570867/149‑1 (MQ=255)
                                                                                        tctcCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCg                                                          >  1:1090395/1‑149 (MQ=255)
                                                                                           ccTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGata                                                       <  1:927499/149‑1 (MQ=255)
                                                                                                  aCGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGTGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGGTGGCACATCTCGTTACCGAGGTCGATATCCAAgg                                                >  2:1202802/1‑148 (MQ=255)
                                                                                                  aCGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGc                                                <  1:997351/149‑1 (MQ=255)
                                                                                                    gTTTATCGGTAATGGAAAGCGACGAAACAAGAGCGTCGATTTTTTTCGCTTTCAGTGGGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGGACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATTGATATCAAAGCca                                              <  2:791569/149‑1 (MQ=255)
                                                                                                      ttATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCcaac                                            <  1:979036/149‑1 (MQ=255)
                                                                                                              aaTGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCAc                                    <  2:794412/149‑1 (MQ=255)
                                                                                                               acagAAAGCGACTAAATAATGGCGTCCATTCTGTTCGCTTTCAGTCAGGGCATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCAcc                                   <  2:323981/146‑1 (MQ=255)
                                                                                                                tGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCt                                  >  1:978755/1‑149 (MQ=255)
                                                                                                                          cgaAATAATAGCGTCGATTTTTTTCGCTTGCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCAAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAACCCAACAAAATCACCTTTAGCATCtt                        >  1:795631/1‑149 (MQ=255)
                                                                                                                              ataataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGa                    <  1:267322/149‑1 (MQ=255)
                                                                                                                              ataataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGa                    <  1:13456/149‑1 (MQ=255)
                                                                                                                               taataGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGCGATCGCAACCAAAGCCACCAAAATCCCCCTTAGCAACttacaat                   >  2:83274/1‑144 (MQ=255)
                                                                                                                                      gTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCGACAAAATCACCTTTAGCATCTTTCGATGAGAACg            >  1:1065042/1‑149 (MQ=255)
                                                                                                                                           tttttttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCg       >  1:545548/1‑149 (MQ=255)
                                                                                                                                              ttttCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGCGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAg    <  2:956681/149‑1 (MQ=255)
                                                                                                                                                ttCGCTTTCAGAGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCGCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGACCGGTACGTAggt  >  2:995665/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTCAGTGAATCCAGCGTTGGCTGAATCGGTGAACCTTTGGCCGCAATCAAACGAGAATCTGCGGCGTACAGCTTGTCGGAGAAGGCAATCTCCTGCTGACGTTTATCGGTAATGGAAAGCGACGAAATAATAGCGTCGATTTTTTTCGCTTTCAGTGAGGGGATCAGCGCGTCAAAGTCACTGGCAACCCAGGTACATTTCACCTGCATCCGTTTGCACATCTCGTTACCGAGATCGATATCAAAGCCAACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGT  >  NC_000913/2427395‑2427687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑