BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,116,406	C→T	100%	M272I (ATG→ATA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,406	0	C	T	100.0%	325.7 / NA	102	M272I (ATG→ATA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (48/54); total (48/54)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 103 total aligned reads displayed.

TTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATA  >  NC_000913/4116261‑4116551

tttcatCGCGCAGGCCCTCAAGGGGTTCGCCTGCCATACACACCGCACCTTCCAATGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGtata                                                                                                                                                <  2:759391/149‑1 (MQ=255)

      cgcgCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCCGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAagcag                                                                                                                                          >  1:621091/1‑149 (MQ=255)

          cAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTGCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAg                                                                                                                                      <  1:225645/149‑1 (MQ=255)

           aGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGt                                                                                                                                     <  1:318230/149‑1 (MQ=255)

             ccACTGAAGGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGc                                                                                                                                   <  2:840459/149‑1 (MQ=255)

               ccTGAATGGATGCCCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCa                                                                                                                                 <  2:540691/148‑1 (MQ=255)

               aCTGAATGGATCGCCCTGCCATAAGCTCCGCCCCTTCCAACGCATAGTTCCCTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCa                                                                                                                                 <  1:731948/149‑1 (MQ=255)

                cTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCAt                                                                                                                                >  2:128350/1‑149 (MQ=255)

                   aaTGGAGGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAgg                                                                                                                             <  1:661889/149‑1 (MQ=255)

                    aTGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGgt                                                                                                                            <  1:804539/149‑1 (MQ=255)

                     tGGATGCGTCTGCCATACACACCGCACCTTCCAACGCATAGTCCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGgtg                                                                                                                           <  1:384666/149‑1 (MQ=255)

                        aTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGttc                                                                                                                        >  2:900806/1‑149 (MQ=255)

                        aTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGttc                                                                                                                        >  2:216432/1‑149 (MQ=255)

                        aTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGttc                                                                                                                        >  2:1271922/1‑149 (MQ=255)

                        aTGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGGGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGttc                                                                                                                        >  2:336455/1‑149 (MQ=255)

                           cgcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGttcttc                                                                                                                     <  1:179205/149‑1 (MQ=255)

                            gcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCg                                                                                                                    <  1:1049409/149‑1 (MQ=255)

                            gcCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCg                                                                                                                    <  1:271418/149‑1 (MQ=255)

                             ccTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCGTCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGc                                                                                                                   >  1:1114220/1‑149 (MQ=255)

                              cTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGca                                                                                                                  >  1:1184147/1‑148 (MQ=255)

                               tGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGGGTTCTTCGCCa                                                                                                                 >  1:72322/1‑149 (MQ=255)

                                 ccATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATc                                                                                                               <  1:866910/149‑1 (MQ=255)

                                   aTAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTCGTCCACACGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGCGTTCCTCAGTATAAAGCAGCCAGTGCCATAGGTGGTCTTCGCCATccc                                                                                                             >  2:211018/1‑149 (MQ=255)

                                      aaCACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCcttc                                                                                                          >  1:368464/1‑149 (MQ=255)

                                          ccGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCGGCAGGCCGGTTTCTGATTTCAACGCTTGCTCACCTGTGGTCATCAGTATAAAGCAGCCAGTGCCAGGGGTGTTCTTCGGCATCCATTCttta                                                                                                      >  1:643877/1‑148 (MQ=255)

                                           cGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCtttac                                                                                                     >  2:850919/1‑147 (MQ=255)

                                            gCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCAc                                                                                                    <  2:1150271/149‑1 (MQ=255)

                                                cgtCCATCGCATAGTTCACTTCGCCAGTCGGGCCGCCGGCGATGGTGGTCAGCCGTCCGTTTTCTGATTTCACCGCGTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGcac                                                                                                <  2:1106980/147‑1 (MQ=255)

                                                      aCGCATGGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACTCACAACTGa                                                                                          <  2:800224/149‑1 (MQ=255)

                                                      aCGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGCGCGCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGGACAACTGa                                                                                          <  1:946466/149‑1 (MQ=255)

                                                             gctCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACa                                                                                   <  1:135614/147‑1 (MQ=255)

                                                              ctCACTTCGCCAGTCGGGCCGGAGGCTATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATAAGTATAAAGCAGCCAGTGCCATAAGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAg                                                                                  <  1:461350/148‑1 (MQ=255)

                                                                 aCTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAgcgc                                                                               >  2:768457/1‑149 (MQ=255)

                                                                  cTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGcgcg                                                                              <  1:900806/149‑1 (MQ=255)

                                                                   ttCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCgg                                                                             >  1:683685/1‑149 (MQ=255)

                                                                    tCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGc                                                                            <  1:483999/149‑1 (MQ=255)

                                                                     cGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTCTCACGCACAACTGACCAAACAGCGCGGcc                                                                           <  1:912604/149‑1 (MQ=255)

                                                                       ccAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCctg                                                                         >  1:766617/1‑149 (MQ=255)

                                                                       ccAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCAGCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACACCCCGGCctg                                                                         >  2:233824/1‑149 (MQ=255)

                                                                        cAGGCGGGCCGCAGGCGATGGTGGGCGGCAGGCCGTTTTCTGATGTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCctgc                                                                        <  2:109492/149‑1 (MQ=255)

                                                                          gtcacgacGCAGGCTATGGTGGTCAGCAGGCCGTTTCCTGGTTTCACCGCCTACACGGCAGTGCTCATCAGTAACAAGCAGCCAGTGCAATACGTGTTCTTCGCCCTTCATTCTTTCCCGCACCACTGACCAAACAGCCCGGCccgctg                                                                      >  2:346282/8‑149 (MQ=255)

                                                                           tCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTgg                                                                     >  2:505473/1‑149 (MQ=255)

                                                                           tCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTgg                                                                     >  2:506099/1‑149 (MQ=255)

                                                                             gggCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCCCGGCCTGCTGGTc                                                                   >  2:1129954/1‑149 (MQ=255)

                                                                               gCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCAc                                                                 >  2:73365/1‑149 (MQ=255)

                                                                                 cGCAGGCGATGGTGGCCGGCCGGCCGTGTTCTGCTTTCACCGCTTTCTCACGATTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCGTTCCCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCg                                                               >  2:601765/1‑149 (MQ=255)

                                                                                   cAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGc                                                             >  1:1246571/1‑149 (MQ=255)

                                                                                        gATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGTTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTACACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATcc                                                        <  1:726022/149‑1 (MQ=255)

                                                                                        gATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATcc                                                        <  1:768457/149‑1 (MQ=255)

                                                                                         aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCGACTGGTCACGGGCGGTccc                                                       >  2:253831/1‑149 (MQ=255)

                                                                                         aTGGTGGTCAGCAGGCCGTTTTCTGATTTCACAGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATccc                                                       >  2:404935/1‑149 (MQ=255)

                                                                                           ggtggtCCGCCGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAACGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCGCACAACTGACCAAACTGCGCGGCCTGCTGGTCACCGGCGATCCCgg                                                     <  1:674622/149‑1 (MQ=255)

                                                                                           ggtggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCgg                                                     <  1:535883/149‑1 (MQ=255)

                                                                                            gtggtCAGCAGGCCGTTTTCTTGTTTCACCGCTTTCTCGCCAGTGTTCATCCGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCCCGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGga                                                    <  2:1148743/149‑1 (MQ=255)

                                                                                              ggtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGAGCCCGgaga                                                  >  1:417872/1‑149 (MQ=255)

                                                                                              ggtCAGCAGGCCGTTTTCTGATTTCACCGCTTGCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGgaga                                                  <  1:29827/149‑1 (MQ=255)

                                                                                               gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGTCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGAt                                                 >  2:651155/1‑149 (MQ=255)

                                                                                               gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGCCCAAACAGCGCGGCCTGCAGGTCACCGGCGATCGCGgagct                                                 >  2:924205/1‑147 (MQ=255)

                                                                                               gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGAt                                                 >  1:17961/1‑149 (MQ=255)

                                                                                               gtCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTACCCGGCGATCCCGgagac                                                 >  2:285823/1‑148 (MQ=255)

                                                                                                tCAGCAGGCCGTTTTCTGATTTCCCCGCTTTCTCGCCAGTGGTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGAtt                                                <  2:586512/149‑1 (MQ=255)

                                                                                                   gcagGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCTGCGATCCCGGAGATTGGa                                             >  2:832985/1‑149 (MQ=255)

                                                                                                   gcagGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGa                                             <  2:742512/149‑1 (MQ=255)

                                                                                                   gcagGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGa                                             <  2:1104620/149‑1 (MQ=255)

                                                                                                   gcagCCCGTTTTCTTATTTCACCGCTTTCTCCCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTCCTTCAACGCACAACTGTCCAAACAGCGCGTCCTGCTGGTCACCGGCGATCCCGGAGATTGGa                                             <  1:575455/149‑1 (MQ=255)

                                                                                                     agGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAt                                           <  1:212578/149‑1 (MQ=255)

                                                                                                         cGTTTTCTGATTTCCCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgc                                       <  1:818545/149‑1 (MQ=255)

                                                                                                         cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgc                                       >  1:919869/1‑149 (MQ=255)

                                                                                                         cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTCCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgc                                       >  1:919384/1‑149 (MQ=255)

                                                                                                          gTTTTCTGATTGCACCGCTTCCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATAcgcg                                      <  2:1053178/149‑1 (MQ=255)

                                                                                                           ttttCTGATTTCACCGCTTTCTCGCCAGTGTTCACGAGTCTCTAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGt                                     <  2:912663/149‑1 (MQ=255)

                                                                                                             ttCTGCTTTCTCTGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgc                                   <  2:525252/149‑1 (MQ=255)

                                                                                                              tCTGCTTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCCGCCGGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTAACCAACCCGCCCGGCCTGCGGGTCACCGGCGATCCCGGAGATTGGCATACGCGGgcc                                  >  2:152072/1‑149 (MQ=255)

                                                                                                              tCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgcc                                  >  1:107590/1‑149 (MQ=255)

                                                                                                              tCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGACGTTCAACGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTTCTGGGCACCGTCGATCCCGGAGATTGGAAGACGCGTgcc                                  >  1:1079414/1‑149 (MQ=255)

                                                                                                               cTGATGGCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccg                                 <  1:351929/149‑1 (MQ=255)

                                                                                                                tGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgc                                <  2:1096325/149‑1 (MQ=255)

                                                                                                                tGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgc                                <  1:962546/149‑1 (MQ=255)

                                                                                                                tGATTTCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGACATATGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTgccgc                                >  1:894497/1‑149 (MQ=255)

                                                                                                                   tttCACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGGTCTTCGCCATCCGTTCTTTCACGCACAACTGACCAGACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCtt                             <  1:175883/149‑1 (MQ=255)

                                                                                                                    ttgaCCGCTTTCTCCCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCttt                            <  2:643877/146‑1 (MQ=255)

                                                                                                                    ttCCCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCttt                            <  1:884731/149‑1 (MQ=255)

                                                                                                                      cACCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTgc                          >  1:109572/1‑149 (MQ=255)

                                                                                                                       aCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCCCAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGCTCCCGGAGCTTGGAATACGCGTGCCGCCtttcgc                         >  2:1148672/1‑146 (MQ=255)

                                                                                                                       aCCGCTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTgcc                         >  1:208745/1‑149 (MQ=255)

                                                                                                                         cggtttCTGGGCAGTGCTCATGAGTATAAAGCAGCCAGTGCCATAGCCGTTCTTCTCCATTTCATCTTTCACGCGCAACTGCCCAAACGGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTTGAATACGCGTGCCGCCTTTgccgc                       <  2:142423/146‑1 (MQ=255)

                                                                                                                           cTTTCTCGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGGCTTTGGCGCCa                     >  2:1024719/1‑149 (MQ=255)

                                                                                                                               ctcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGt                 >  1:413319/1‑149 (MQ=255)

                                                                                                                               ctcGCCAGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCCCGGCCTGCTTGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGt                 <  1:688567/149‑1 (MQ=255)

                                                                                                                                  gCCAGCGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAg              <  1:1031480/149‑1 (MQ=255)

                                                                                                                                   ccAGTGTTCATCAGTATAAAGCAGCCAGTGGCATAGGTGTTCTTAGGACAGCCGTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGt             <  1:940231/149‑1 (MQ=255)

                                                                                                                                     aGTGTTCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCt           <  1:521586/149‑1 (MQ=255)

                                                                                                                                         ttCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc       <  1:505473/149‑1 (MQ=255)

                                                                                                                                         ttCATCAGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc       <  1:506099/149‑1 (MQ=255)

                                                                                                                                         ttCATCAGTATAAAGCAGCCAATGCCATAAGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGAAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCGGAcc       >  1:919270/1‑149 (MQ=255)

                                                                                                                                            atcaGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGta    >  1:523548/1‑149 (MQ=255)

                                                                                                                                            atcaGTATAAAGCAGCCAGTGCCATAGGTCTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGta    >  1:914192/1‑149 (MQ=255)

                                                                                                                                             tcaGTATAAAGCCGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat   <  2:484149/149‑1 (MQ=255)

                                                                                                                                              caGTATAAAGCAGCCATTGCCATGCGTGTTCTTCGCCATCCCTTCGTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGCTCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtata  <  2:904242/149‑1 (MQ=255)

                                                                                                                                              caGTATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtaac  >  2:1098295/1‑147 (MQ=255)

TTTCATCGCGCAGCCACTGAATGGATGCGCCTGCCATAAACACCGCACCTTCCAACGCATAGTTCACTTCGCCAGTCGGGCCGCAGGCGATGGTGGTCAGCAGGCCGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATA  >  NC_000913/4116261‑4116551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`