Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,182,928 A→T 100% E562V (GAA→GTA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9280AT100.0% 362.2 / ‑6.7 112E562V (GAA→GTA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (67/44);  minor base C (0/1);  total (67/45)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 112 total aligned reads displayed.

TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCG  >  NC_000913/4182783‑4183071
                                                                                                                                                 |                                                                                                                                               
tttATGGACGAGAACAACCCGCTGTCTGAGTTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAAc                                                                                                                                              <  1:820063/149‑1 (MQ=255)
tttATGGACCAGACCAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAAc                                                                                                                                              <  1:999160/149‑1 (MQ=255)
        ccAGACCAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGGAGGCTTCCAAGTTCGAGACGTACACCCGACTCACTACGGGCGCGTATGTCCAATCGTAACCCCTGAAg                                                                                                                                      >  1:1018837/1‑149 (MQ=255)
        ccAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAg                                                                                                                                      >  1:299158/1‑149 (MQ=255)
          aGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGATGTCAATGCCGGGGCCCCATTATATCTCGAAGTTCGAGACGTACACCGGACTCACTACGTTCGCGTATGTCCAATCGTAACCCCTGAAGGt                                                                                                                                    >  1:629133/1‑149 (MQ=255)
               aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCGGaa                                                                                                                               >  2:259594/1‑149 (MQ=255)
                 cccgtcgTCTGAGATTACGCACAAACGGCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACa                                                                                                                             <  2:945852/143‑1 (MQ=255)
                  ccGCTGTCTGAGATTACGCACAACCGTCGTATCGCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACAt                                                                                                                            <  2:653468/149‑1 (MQ=255)
                  ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAGTGGTAACCCCTGAAGGTCGGcacag                                                                                                                            >  2:778398/1‑148 (MQ=255)
                  ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACAt                                                                                                                            <  2:68458/149‑1 (MQ=255)
                  ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGATCCGGACAt                                                                                                                            >  2:617232/1‑149 (MQ=255)
                  ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGGCGCGGATGTCCAATCGTAACCCCTGAAGGTCCGAACAt                                                                                                                            >  1:798260/1‑149 (MQ=255)
                    gctggctgTGATTACGCACAAACGGCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCg                                                                                                                          <  2:1152289/149‑1 (MQ=255)
                      tGTCTGAGATTACGCACAAACGTCGTATCCCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGt                                                                                                                        <  1:697787/149‑1 (MQ=255)
                        tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCt                                                                                                                      >  1:1107003/1‑149 (MQ=255)
                        tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGGCt                                                                                                                      >  1:1130179/1‑149 (MQ=255)
                         cTGAGCTTACGCACAAACGTCGTATCTCCGCACTCGGCCCCGGAGGCCTGTCCCGTGACCGCGCAGGCTCCGAAGTTCGAGCAGTACACCCGTCTCACTACGGTTGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTg                                                                                                                     <  1:101879/149‑1 (MQ=255)
                               ttACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGCGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCGGAAGGTCCGGACATCGGTCTGAGCAAc                                                                                                               >  2:345327/1‑149 (MQ=255)
                                   gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc                                                                                                           >  1:1296889/1‑149 (MQ=255)
                                       aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                                                       >  2:934662/1‑149 (MQ=255)
                                        aaCGTCGTATCTCCGCACTCGGCCCAGGCGTTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTAGCGTATGTCCAATCGTAACCCCTGAAGGTCCGCAAAACGGCCGGATCAAccctcttgcc                                                                                                      >  1:673490/1‑145 (MQ=255)
                                        aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc                                                                                                      <  1:161271/149‑1 (MQ=255)
                                        aaCGGCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc                                                                                                      <  2:1296263/149‑1 (MQ=255)
                                          cgtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt                                                                                                    <  1:505629/149‑1 (MQ=255)
                                             cgtCTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAGGTTCGAGACGTACACCCGACTCACTCCGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa                                                                                                 <  2:489940/149‑1 (MQ=255)
                                             cgtATCTCCGCACTCCGCCCAGGCGGGCTGACCCGTGAACGTGCAGGCGTGGAAGTTCGAGACGTACACCCGACCCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTa                                                                                                 <  2:504171/149‑1 (MQ=255)
                                              gtATCTCCGCGCTCGGCCCAGGCCGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                                <  2:789585/149‑1 (MQ=255)
                                              gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                                >  1:1049164/1‑149 (MQ=255)
                                              gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                                                                >  1:118025/1‑149 (MQ=255)
                                               tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACg                                                                                               >  1:1015268/1‑149 (MQ=255)
                                                 tctcCCCACTCATCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAACGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                                                             <  1:1035728/149‑1 (MQ=255)
                                                       cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACt                                                                                       >  1:579014/1‑149 (MQ=255)
                                                            ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTTTGATAATCTCGCTGTCCGTGTACGCACAGACTAACGa                                                                                  <  1:956878/149‑1 (MQ=255)
                                                            ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa                                                                                  >  1:725614/1‑149 (MQ=255)
                                                            ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGa                                                                                  >  2:1011058/1‑149 (MQ=255)
                                                               cgcggCGGTCTGACCCGTGAGCGTGGAGGCTTCGAAGTGCGGGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTACCCCCTGAAGGTCCGAACAGCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATa                                                                               <  2:89701/146‑1 (MQ=255)
                                                                  ggCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                                                            <  1:1211501/149‑1 (MQ=255)
                                                                     ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt                                                                         >  1:1092699/1‑149 (MQ=255)
                                                                       tCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTCACGAATACGGCTTcc                                                                       >  1:1036591/1‑149 (MQ=255)
                                                                       tCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTcc                                                                       <  2:1015268/149‑1 (MQ=255)
                                                                         tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTGCGCGCAGACTAACGAATACGGCTTCCtt                                                                     >  1:323481/1‑149 (MQ=255)
                                                                         tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                     >  1:1041522/1‑149 (MQ=255)
                                                                         tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCtt                                                                     >  1:1113116/1‑149 (MQ=255)
                                                                           aCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga                                                                   >  1:476974/1‑149 (MQ=255)
                                                                           aCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga                                                                   >  1:926009/1‑149 (MQ=255)
                                                                            cccGTGAACGTGCAGGCTTCGAAGTTCGAGCCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCCCACACTAACGAATACGCCTTCCCTgag                                                                  >  2:296911/1‑149 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTCCGGTCGCGAATGTCCAATCGTAACCCCTGAAGGTCCGAACAGCGGTCTGATCAACTCTCTGTCCGGGTACGCACAGACTAACGAATCCAGCCTCCttagga                                                                 >  2:250286/1‑145 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTAgaga                                                                 >  1:436624/1‑149 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACGCTCTGTCCGTGTACGCACAGACTAACGAATACGGCGTCCCTgaga                                                                 >  2:428125/1‑149 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGAATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga                                                                 >  2:841519/1‑149 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGCCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGGACACCGCGAAGGTCGACTCTCTGTCCGTGTAGTCACAGACTAACGAAACCGGCTTCCATgccc                                                                 >  2:1082306/1‑146 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTAAACAAGACCCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAAGTCCGAACATCGGTCTCATCAACTCTCCATCCGTGTACGCACAGACCAACCAATCCCACTCCCTTgaga                                                                 >  2:339542/1‑149 (MQ=255)
                                                                             ccGTGAACGTGCAGGCTTCGAAGTTCGAGAAGTGCCCGCCACCCGCTACGTCCGCGTATGTACAATCGTAACCCCCGAACGTCCTAACAGCGGTCTGCTCAACTCCCTGTCCGTGTACGGACAGACTGACGAATACCGCTTCCTTgaga                                                                 >  2:339547/1‑149 (MQ=255)
                                                                              cGTGAACGTGTAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGAACAGACTAACGAATACGGCTTCCTTGAGAc                                                                >  2:1268141/1‑149 (MQ=255)
                                                                              cGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCttaggac                                                                >  2:40843/1‑144 (MQ=255)
                                                                              cGTGAACGAACAAGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGCGTACGCACAGACTAACGAATACGGCCTCCTTGAGAc                                                                >  2:801130/1‑149 (MQ=255)
                                                                               gTGAACGTGCAGGCTTCGAAGTTCGAGACGCACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACTTCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCCTGCGACt                                                               >  2:892220/1‑149 (MQ=255)
                                                                                tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGCCCGTGAACGCACAGACTAACGAATACGGCTTCCTTGAGACTc                                                              >  1:563407/1‑149 (MQ=255)
                                                                                      tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                        >  2:959494/1‑149 (MQ=255)
                                                                                      tGCAGCCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCTTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc                                                        <  2:965882/149‑1 (MQ=255)
                                                                                         aGGCTTCGAAGTTCGAGACGTACACCCGCCTCACCACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa                                                     <  1:753391/149‑1 (MQ=255)
                                                                                           ggttggAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   <  2:1207376/144‑1 (MQ=255)
                                                                                           gCTTGGAAGTTCGAGACGTACACGGGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   <  1:467403/149‑1 (MQ=255)
                                                                                           gCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGGCTCCGTATCGTaaa                                                   >  1:324455/1‑149 (MQ=255)
                                                                                           gCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   <  1:1292773/149‑1 (MQ=255)
                                                                                           gCTTCGAAGTTCGAGACGTACACCCAACTCGCTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaag                                                   >  1:671530/1‑148 (MQ=255)
                                                                                           gCTTCGAAGTTCGAGACGTACACCCAACTCGCTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   >  1:671685/1‑149 (MQ=255)
                                                                                           gCTTCGAAGGCCGGGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   <  1:77938/149‑1 (MQ=255)
                                                                                           ctttCGAAGTCCGAGACCTACACCCTACTCACTCCGTTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCGGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa                                                   <  2:1207519/147‑1 (MQ=255)
                                                                                             ttCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGCCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGt                                                 >  1:6650/1‑149 (MQ=255)
                                                                                                gAAGTTCGATACGCACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc                                              <  1:168130/149‑1 (MQ=255)
                                                                                                gAAGTTCGAGACGGACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGATTAACGTATACGGCTTCCTTGAGACTCCCTATCGTAAAGTGAc                                              >  1:915177/1‑149 (MQ=255)
                                                                                                    ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGGTCCACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGCGCCTCCGTCTCGTACAGTGACCAAc                                          >  2:109070/1‑149 (MQ=255)
                                                                                                    ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                          >  1:1211696/1‑149 (MQ=255)
                                                                                                    ttCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGCCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc                                          >  1:631200/1‑149 (MQ=255)
                                                                                                     tCGAGACGTACACCCGACTCACTACGGTCGCGTATGTACAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATAGTAAAGTGACCGACg                                         >  1:261641/1‑149 (MQ=255)
                                                                                                      cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACgg                                        <  2:1107003/149‑1 (MQ=255)
                                                                                                            gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTGCGTTGAGACTCCGTATCGTAAAGTGACCGACGGtgttgt                                  >  1:1208326/1‑149 (MQ=255)
                                                                                                             tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGACTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTa                                 >  1:1057857/1‑149 (MQ=255)
                                                                                                              ccacCCGACCCACCACGGTCGGGTATGTCCAACCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCACCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTaa                                <  2:689210/148‑1 (MQ=255)
                                                                                                              acacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTaa                                <  1:223741/149‑1 (MQ=255)
                                                                                                              acacCCGACTCACGACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTaa                                <  1:778398/149‑1 (MQ=255)
                                                                                                               cgcccGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAAc                               <  2:1296200/147‑1 (MQ=255)
                                                                                                               cacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAAc                               <  2:1296889/149‑1 (MQ=255)
                                                                                                                acCCGACTCACTACGGTCGCGTATGTCACTTCATCAGGATAGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGATTACGGCTTCCTTGAGACACCGTACCGTAAAGTGACCGACGGTGTTGTAACt                              >  1:1284148/1‑149 (MQ=255)
                                                                                                                          cTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTc                    >  2:1205092/1‑149 (MQ=255)
                                                                                                                          cTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAAGAAATTc                    >  1:980580/1‑149 (MQ=255)
                                                                                                                            aCGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCAc                  <  2:1165868/149‑1 (MQ=255)
                                                                                                                             cGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAAATCTCTGTCCGTGTACGCAACGACTACCGAATCCGGATTCCTTAACAATCCGGACCGTAAAATGACCGACGGTGTGGTACCTGACGAAATTCAcc                 >  2:354108/1‑148 (MQ=255)
                                                                                                                                tCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAcc              >  2:520698/1‑149 (MQ=255)
                                                                                                                                 cgcgTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCt             <  1:583749/149‑1 (MQ=255)
                                                                                                                                  gcgTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGCAATTCACTAcccg            >  2:628926/1‑147 (MQ=255)
                                                                                                                                     tATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCt         <  1:438238/149‑1 (MQ=255)
                                                                                                                                      aTGTCCAGTCGTAGCCCGTGACGGTCCGAACATCGGTCTGAGCAACTCTCTGTCCGTGTACGCACAGACTAACGAATTCGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTg        <  1:98856/149‑1 (MQ=255)
                                                                                                                                      aTGTACAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTg        <  1:1132824/149‑1 (MQ=255)
                                                                                                                                         tCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTa     >  2:1258839/1‑149 (MQ=255)
                                                                                                                                         tCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGGAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGCATGCTa     >  2:852970/1‑149 (MQ=255)
                                                                                                                                         gccAATCTCAACCCCCGAAGGTCCCAACATCGGTCCGATCAACGCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAACGCAACCTGCGGCTGGGGAACTGACGAAATTCACTACCTGTCTGCTa     <  1:313386/148‑1 (MQ=255)
                                                                                                                                          ccAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTAt    >  1:131197/1‑149 (MQ=255)
                                                                                                                                            aaTCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTCCGCACAGGCTAACCAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAGGAAATTCACTACCTGTCTGCTATCg  >  1:794296/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCG  >  NC_000913/4182783‑4183071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑