| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,182,928 | A→T | E562V (GAA→GTA) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,182,928 | 0 | A | T | 97.1% | 99.7 / NA | 34 | E562V (GAA→GTA) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (20/13); minor base C (0/1); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTG > NC_000913/4182783‑4183065 | tttATGGACCAGAACAACCCGCTGTCTGAGCTTACGCACAAACGTCGTATCTCCGCACGCGGCCCAGGCCGACTCACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCCCTCCGGTCGCGTATGTCCCATCGTAAc > 1:62274/1‑149 (MQ=255) ggACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTCAACCCGACGCACTACGGTCGCGCATGTCCAAGCGTAACCCCTg > 2:147212/1‑149 (MQ=255) gaccagaccaCCCCGCTGTCTGAGATTACGCACCAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGa < 1:155119/149‑1 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGa > 2:379819/1‑149 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTTCGGTCGCGTATGTCCAATCGTAACCCCTGAAgg < 1:287559/149‑1 (MQ=255) ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACAt > 1:202218/1‑149 (MQ=255) tGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCATAACCCCTGAAGGTCCGAACATCGGt < 1:92995/149‑1 (MQ=255) tCTGAGATTACGCACAAACGTCGTATCTCCGCCCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCt < 2:427602/149‑1 (MQ=255) gagaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCGGAt > 1:63002/1‑149 (MQ=255) aaagtcgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc > 2:99157/4‑149 (MQ=255) aaCGTTGTATCCCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTCCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc < 1:245887/149‑1 (MQ=255) aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAATATCGGTCTGATCAACTCTCTGTcc < 1:331972/149‑1 (MQ=255) tcgtATCTCCGCACTCGGACCAGACAGTCTGACCCGTCGACGTACAGGCTTCGAATTTCTAGGCGTACACCCGACTCACGACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt > 2:262063/1‑149 (MQ=255) tcgtATCTCAGCACTCGGCCCAGAAAGTCTGGCCAGTGAACGTACGGGCGTCGAGGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt > 2:262049/1‑149 (MQ=255) tcgtATCGCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCTAAGTTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTCAAGGTCCGAACATCGGTCTGACCGACTCTCAGTCCgtgt > 2:142395/1‑149 (MQ=255) tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca > 1:431876/1‑149 (MQ=255) tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcaca > 1:390149/1‑149 (MQ=255) tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGATCCGAACATCGGCCTGATCAACTCTCGGTCCGGGTACGCACAGACTAAc > 2:352112/1‑149 (MQ=255) gggcgTCTGACCCGGGAACGTGCAGGCTTCGAAGCTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg < 2:267987/145‑1 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTGCGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGCACGCACAGCCTACCGAATACGGCTTcc > 1:254748/1‑149 (MQ=255) gACCCGTGAACGGGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg < 2:447207/149‑1 (MQ=255) ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga > 2:422590/1‑149 (MQ=255) tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTGGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAAGGAATACGGCTTCCTTGGGACTc > 2:157076/1‑149 (MQ=255) tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTc > 1:374476/1‑149 (MQ=255) gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGAACAAATCTCTGTCCGTGTACGCACAGACTAACGAATACGGCGTCCTTGAGACTcc > 1:393532/1‑149 (MQ=255) tcgtCGAAGTTCGATACGTACACCCGTCTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa < 2:122776/146‑1 (MQ=255) tACACCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTa > 2:282884/1‑149 (MQ=255) acacCCGACTCACTACGGTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTaa < 1:363211/149‑1 (MQ=255) ggTGGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCGGTCCGTGTAGGCACAGACTAACGAATACGGCTGCCTTGCGACTCCCGATCTTAAAGTGAACGACGCTATTTTTACTGAGCAAATACCCTa > 2:47920/1‑149 (MQ=255) gTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAc < 1:435511/149‑1 (MQ=255) gTCGCGTATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTAc < 1:435507/149‑1 (MQ=255) cgcgTATGTCCAACCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCt < 1:319399/149‑1 (MQ=255) tATGTCCAATCGTAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATCCACTACCTGCCt > 2:168556/1‑149 (MQ=255) aTGTCCAATCGCAACCCCTGAAGGTCCGAACATCGGTCTGACCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTg < 1:2857/149‑1 (MQ=255) | TTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTG > NC_000913/4182783‑4183065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |